JP7371119B2 - グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法 - Google Patents

グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法 Download PDF

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JP7371119B2
JP7371119B2 JP2021562185A JP2021562185A JP7371119B2 JP 7371119 B2 JP7371119 B2 JP 7371119B2 JP 2021562185 A JP2021562185 A JP 2021562185A JP 2021562185 A JP2021562185 A JP 2021562185A JP 7371119 B2 JP7371119 B2 JP 7371119B2
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amino acid
glutamine
polypeptide
microorganism
seq
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JP2022529297A (ja
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チン チェ，ス
リ，イムサン
キム，ヘヨン
ソ キム，ピョン
ウ リ，カン
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CJ CheilJedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021562185A 2020-03-04 2021-03-04 グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法 Active JP7371119B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023090522A JP2023110008A (ja) 2020-03-04 2023-05-31 グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2020-0027322 2020-03-04
KR1020200027322A KR102198072B1 (ko) 2020-03-04 2020-03-04 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법
PCT/KR2021/002652 WO2021177731A1 (ko) 2020-03-04 2021-03-04 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법

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JP2023090522A Division JP2023110008A (ja) 2020-03-04 2023-05-31 グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法

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JP2022529297A JP2022529297A (ja) 2022-06-20
JP7371119B2 true JP7371119B2 (ja) 2023-10-30

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JP2021562185A Active JP7371119B2 (ja) 2020-03-04 2021-03-04 グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法
JP2023090522A Pending JP2023110008A (ja) 2020-03-04 2023-05-31 グルタミンシンテターゼ変異型ポリペプチド及びそれを用いたｌ－グルタミン生産方法

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US (1) US12270061B2 (https=)
EP (1) EP3954767A4 (https=)
JP (2) JP7371119B2 (https=)
KR (1) KR102198072B1 (https=)
CN (1) CN114375332B (https=)
BR (1) BR112021022223B1 (https=)
WO (1) WO2021177731A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102198072B1 (ko) 2020-03-04 2021-01-04 씨제이제일제당 주식회사 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법
WO2022108383A1 (ko) 2020-11-20 2022-05-27 씨제이제일제당 (주) L-글루타민 생산능이 향상된 미생물 및 이를 이용한 l-글루타민 생산 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002300887A (ja) 2001-02-05 2002-10-15 Ajinomoto Co Inc 発酵法によるｌ−グルタミンの製造法及びｌ−グルタミン生産菌
JP2004283167A (ja) 2003-03-03 2004-10-14 Ajinomoto Co Inc 発酵法によるｌ−アルギニン又はｌ−リジンの製造法
CN1884501A (zh) 2006-06-29 2006-12-27 清华大学 谷氨酰胺合成酶及其专用表达工程菌与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060269975A1 (en) * 1999-07-16 2006-11-30 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis
RU2230114C2 (ru) * 2001-11-30 2004-06-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Мутантная глутаминсинтетаза, фрагмент днк, штамм escherichia coli - продуцент l-глутамина и способ получения l-аминокислот
JP2004187684A (ja) * 2002-11-26 2004-07-08 Ajinomoto Co Inc Ｌ−グルタミンの製造法及びｌ−グルタミン生産菌
EP1460128B1 (en) 2003-03-03 2016-11-30 Ajinomoto Co., Inc. Method for producing L-arginine or L-lysine by fermentation
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
JP5048996B2 (ja) 2006-11-10 2012-10-17 昭和電工株式会社 加工性に優れた耐摩耗性アルミニウム合金材およびその製造方法
PT2814965T (pt) * 2012-02-16 2018-05-23 Syngenta Participations Ag Proteínas pesticidas manipuladas
JP2015513406A (ja) * 2012-03-12 2015-05-14 ハンファ ケミカル コーポレーション 改変グルタミン合成酵素をコードするポリヌクレオチドを含む発現ベクターおよびこれを用いた標的タンパク質の製造方法
MY179341A (en) * 2014-04-30 2020-11-04 Evonik Operations Gmbh Method for producing l-amino acids using an alkaliphilic bacteria
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
MY186296A (en) 2016-08-31 2021-07-06 Cj Cheiljedang Corp Novel promoter and use thereof
KR102198072B1 (ko) 2020-03-04 2021-01-04 씨제이제일제당 주식회사 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002300887A (ja) 2001-02-05 2002-10-15 Ajinomoto Co Inc 発酵法によるｌ−グルタミンの製造法及びｌ−グルタミン生産菌
JP2004283167A (ja) 2003-03-03 2004-10-14 Ajinomoto Co Inc 発酵法によるｌ−アルギニン又はｌ−リジンの製造法
CN1884501A (zh) 2006-06-29 2006-12-27 清华大学 谷氨酰胺合成酶及其专用表达工程菌与应用

Also Published As

Publication number Publication date
JP2022529297A (ja) 2022-06-20
CN114375332B (zh) 2024-01-12
WO2021177731A1 (ko) 2021-09-10
CN114375332A (zh) 2022-04-19
JP2023110008A (ja) 2023-08-08
EP3954767A1 (en) 2022-02-16
US12270061B2 (en) 2025-04-08
BR112021022223B1 (pt) 2023-09-26
BR112021022223A2 (pt) 2022-09-20
KR102198072B1 (ko) 2021-01-04
US20220396815A1 (en) 2022-12-15
EP3954767A4 (en) 2022-08-03

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