BR112021022223A2 - Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina - Google Patents

Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina

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Publication number
BR112021022223A2
BR112021022223A2 BR112021022223A BR112021022223A BR112021022223A2 BR 112021022223 A2 BR112021022223 A2 BR 112021022223A2 BR 112021022223 A BR112021022223 A BR 112021022223A BR 112021022223 A BR112021022223 A BR 112021022223A BR 112021022223 A2 BR112021022223 A2 BR 112021022223A2
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BR
Brazil
Prior art keywords
glutamine
producing
modified polypeptide
polynucleotide
organism
Prior art date
Application number
BR112021022223A
Other languages
English (en)
Other versions
BR112021022223B1 (pt
Inventor
Jin Choi Su
Lee Imsang
KIM Heeyeong
Soo Kim Byeong
Woo Lee Kwang
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of BR112021022223A2 publication Critical patent/BR112021022223A2/pt
Publication of BR112021022223B1 publication Critical patent/BR112021022223B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
    • C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

POLIPEPTÍDEO MODIFICADO, POLINUCLEOTÍDEO, MICRO-ORGANISMO E MÉTODO PARA PRODUZIR L-GLUTAMINA. A presente invenção se refere a um polipeptídeo modificado de glutamina sintetase com atividade melhorada, e a um método para produzir L-glutamina usando o mesmo. Uma vez que a produção de L-glutamina pode ser aumentada usando o polipeptídeo modificado inédito sem uma diminuição na taxa de crescimento, comparado às cepas tipo selvagem com atividade de glutamina sintetase, o polipeptídeo modificado pode ser amplamente usado para produção em massa de L-glutamina.
BR112021022223-5A 2020-03-04 2021-03-04 Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina BR112021022223B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2020-0027322 2020-03-04
KR1020200027322A KR102198072B1 (ko) 2020-03-04 2020-03-04 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법
PCT/KR2021/002652 WO2021177731A1 (ko) 2020-03-04 2021-03-04 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법

Publications (2)

Publication Number Publication Date
BR112021022223A2 true BR112021022223A2 (pt) 2022-09-20
BR112021022223B1 BR112021022223B1 (pt) 2023-09-26

Family

ID=74127205

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021022223-5A BR112021022223B1 (pt) 2020-03-04 2021-03-04 Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina

Country Status (7)

Country Link
US (1) US20220396815A1 (pt)
EP (1) EP3954767A4 (pt)
JP (2) JP7371119B2 (pt)
KR (1) KR102198072B1 (pt)
CN (1) CN114375332B (pt)
BR (1) BR112021022223B1 (pt)
WO (1) WO2021177731A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102198072B1 (ko) * 2020-03-04 2021-01-04 씨제이제일제당 주식회사 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법
JP2023550131A (ja) 2020-11-20 2023-11-30 シージェイ チェイルジェダン コーポレーション Ｌ－グルタミン生産能が向上した微生物及びそれを用いたｌ－グルタミン生産方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060269975A1 (en) * 1999-07-16 2006-11-30 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis
JP4560998B2 (ja) * 2001-02-05 2010-10-13 味の素株式会社 発酵法によるｌ−グルタミンの製造法及びｌ−グルタミン生産菌
RU2230114C2 (ru) * 2001-11-30 2004-06-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Мутантная глутаминсинтетаза, фрагмент днк, штамм escherichia coli - продуцент l-глутамина и способ получения l-аминокислот
JP2004187684A (ja) * 2002-11-26 2004-07-08 Ajinomoto Co Inc Ｌ−グルタミンの製造法及びｌ−グルタミン生産菌
JP4576850B2 (ja) * 2003-03-03 2010-11-10 味の素株式会社 発酵法によるｌ−アルギニン又はｌ−リジンの製造法
EP1460128B1 (en) * 2003-03-03 2016-11-30 Ajinomoto Co., Inc. Method for producing L-arginine or L-lysine by fermentation
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
CN100392075C (zh) * 2006-06-29 2008-06-04 清华大学 谷氨酰胺合成酶及其专用表达工程菌与应用
JP5048996B2 (ja) 2006-11-10 2012-10-17 昭和電工株式会社 加工性に優れた耐摩耗性アルミニウム合金材およびその製造方法
BR112014020164B1 (pt) * 2012-02-16 2022-09-13 Syngenta Participations Ag Polipeptídeo pesticida vip3 manipulado, molécula de ácido nucleico, vetor e composição
EP2825641A4 (en) * 2012-03-12 2015-09-30 Hanwha Chemical Corp EXPRESSION VECTOR WITH A POLYNUCLEOTIDE CODING FOR A MODIFIED GLUTAMINE SYNTHETASE AND METHOD FOR PRODUCING A TARGET PROTEIN THEREFOR
MY179341A (en) * 2014-04-30 2020-11-04 Evonik Operations Gmbh Method for producing l-amino acids using an alkaliphilic bacteria
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
JP6679803B2 (ja) 2016-08-31 2020-04-15 シージェイ チェイルジェダン コーポレーション 新規プロモーター及びその用途
KR102198072B1 (ko) * 2020-03-04 2021-01-04 씨제이제일제당 주식회사 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법

Also Published As

Publication number Publication date
JP7371119B2 (ja) 2023-10-30
JP2023110008A (ja) 2023-08-08
US20220396815A1 (en) 2022-12-15
EP3954767A4 (en) 2022-08-03
CN114375332B (zh) 2024-01-12
CN114375332A (zh) 2022-04-19
WO2021177731A1 (ko) 2021-09-10
EP3954767A1 (en) 2022-02-16
KR102198072B1 (ko) 2021-01-04
BR112021022223B1 (pt) 2023-09-26
JP2022529297A (ja) 2022-06-20

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Legal Events

Date Code Title Description
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 04/03/2021, OBSERVADAS AS CONDICOES LEGAIS