BR112018009729A2 - método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico - Google Patents
método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleicoInfo
- Publication number
- BR112018009729A2 BR112018009729A2 BR112018009729A BR112018009729A BR112018009729A2 BR 112018009729 A2 BR112018009729 A2 BR 112018009729A2 BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A2 BR112018009729 A2 BR 112018009729A2
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant polypeptide
- filamentous fungus
- producing
- nucleic acid
- myceliophthora thermophila
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/2488—Mannanases
- C12N9/2491—Beta-mannosidase (3.2.1.25), i.e. mannanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03008—3-Phytase (3.1.3.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21063—Oryzin (3.4.21.63)
Abstract
a presente invenção diz respeito a um método de produção de um polipeptídeo recombinante em um fungo filamentoso que é geneticamente modificado para diminuir ou eliminar a atividade do regulador da celulase 2 (clr2) e para expressar o referido polipeptídeo recombinante. o método diz respeito ainda a um fungo filamentoso myceliophthora thermophila que é geneticamente modificado para diminuir ou eliminar a atividade do clr2 e ao uso deste fungo filamentoso na produção de um polipeptídeo recombinante.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15197497 | 2015-12-02 | ||
PCT/EP2016/079526 WO2017093450A1 (en) | 2015-12-02 | 2016-12-02 | Method of producing proteins in filamentous fungi with decreased clr2 activity |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112018009729A2 true BR112018009729A2 (pt) | 2018-11-21 |
BR112018009729A8 BR112018009729A8 (pt) | 2019-02-26 |
Family
ID=54838169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018009729A BR112018009729A8 (pt) | 2015-12-02 | 2016-12-02 | método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico |
Country Status (9)
Country | Link |
---|---|
US (1) | US11299522B2 (pt) |
EP (1) | EP3384002A1 (pt) |
JP (1) | JP2019503168A (pt) |
KR (1) | KR20180084135A (pt) |
CN (1) | CN108291189A (pt) |
BR (1) | BR112018009729A8 (pt) |
CA (1) | CA3004990A1 (pt) |
MX (1) | MX2018006535A (pt) |
WO (1) | WO2017093450A1 (pt) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3384002A1 (en) | 2015-12-02 | 2018-10-10 | Basf Se | Method of producing proteins in filamentous fungi with decreased clr2 activity |
BR112018010310A2 (pt) | 2015-12-02 | 2018-12-04 | Basf Se | método de produção de polipeptídeos recombinantes, fungo filamentoso, uso de construção de ácido nucleico e uso de fungo filamentoso |
WO2019038623A1 (en) | 2017-08-21 | 2019-02-28 | Dyadic International Inc. | PRODUCTION OF AN INFLUENZA VACCINE IN MYCELIOPHTHORA THERMOPHILA |
CN110042116A (zh) * | 2019-04-30 | 2019-07-23 | 贵州大学 | 一种稻瘟菌生物钟控制基因MoCCG4的重组载体和表达方法 |
CN112813091A (zh) * | 2021-02-03 | 2021-05-18 | 山东农业大学 | Nat1基因作为筛选标记在卵菌遗传转化中的应用 |
CN113151264A (zh) * | 2021-03-11 | 2021-07-23 | 上海中溶科技有限公司 | 一种利用正向调控基因构建高产纤维素酶菌株的方法及应用 |
Family Cites Families (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0635574T3 (da) | 1993-07-23 | 2003-08-11 | Dsm Ip Assets Bv | Selektionsmarkørgenfri rekombinantstammer, en fremgangsmåde til fremstilling af disse og anvendelse af disse stammer |
PL336345A1 (en) | 1997-04-11 | 2000-06-19 | Dsm Nv | Genic conversion as a tool for constructing recombined filiform fungi |
AU771539C (en) | 1998-10-06 | 2005-01-13 | Dyadic International (Usa), Inc. | Transformation system in the field of filamentous fungal hosts |
EP2505651A3 (en) * | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
DK2408910T3 (en) * | 2009-03-16 | 2018-07-30 | Danisco Us Inc | CHRYSOSPORIUM LUCKNOWENSE PROTEIN MANUFACTURING SYSTEM |
US20120005812A1 (en) | 2010-07-06 | 2012-01-12 | Paul Corzatt | Insect protective garment |
WO2012048334A2 (en) | 2010-10-08 | 2012-04-12 | Dyadic International (Usa) Inc. | Novel fungal proteases |
BR112014001452A2 (pt) * | 2011-07-21 | 2017-02-21 | Univ California | fatores de transcrição para a produção de enzima de degradação de celulose |
WO2013048661A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
WO2014202620A2 (en) * | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
MY181068A (en) | 2013-09-25 | 2020-12-17 | Basf Se | Recombinant microorganism for improved production of fine chemicals |
BR112016012430A2 (pt) | 2013-12-09 | 2017-09-26 | Basf Se | métodos de produção de riboflavina e de organismo que acumula riboflavina, organismo que acumula riboflavina, usos de gene e de um organismo, e, produto de riboflavina |
KR20210133323A (ko) | 2013-12-13 | 2021-11-05 | 바스프 에스이 | 정밀 화학물질의 개선된 생산을 위한 재조합 미생물 |
WO2015092599A1 (en) | 2013-12-17 | 2015-06-25 | Basf Se | Improved process for the production of gamma-aminobutyric acid (gaba) |
WO2015092576A1 (en) | 2013-12-17 | 2015-06-25 | Basf Se | Improved process for the production of gamma-aminobutyric acid (gaba) |
CN106164249A (zh) | 2014-02-07 | 2016-11-23 | 巴斯夫欧洲公司 | 用于基于蔗糖的改善精细化学品产生的修饰微生物 |
KR102304834B1 (ko) | 2014-02-07 | 2021-09-27 | 바스프 에스이 | 숙신산 생산을 위한 개선된 미생물 |
WO2015118111A2 (en) | 2014-02-07 | 2015-08-13 | Basf Se | Modified microorganism with improved biomass separation behaviour |
WO2015135980A1 (en) | 2014-03-11 | 2015-09-17 | Basf Se | The use of polymers to improve the rheology of base suspensions during fermentation |
CA2942990A1 (en) | 2014-03-19 | 2015-09-24 | Basf Se | The use of glycerol with limited feed of carbohydrates for fermentation |
WO2015169920A1 (en) | 2014-05-08 | 2015-11-12 | Basf Se | Genetically modified microorganism for improved production of fine chemicals on sucrose |
WO2015177674A1 (en) | 2014-05-21 | 2015-11-26 | Basf Se | Gene cluster for biosynthesis of cornexistin and hydroxycornexistin |
WO2016030373A1 (en) | 2014-08-28 | 2016-03-03 | Basf Se | Modified microorganism for improved production of fine chemicals on sucrose |
WO2016091980A1 (en) | 2014-12-10 | 2016-06-16 | Basf Se | Method of removing dna from biotechnological products |
TW201707740A (zh) | 2015-06-03 | 2017-03-01 | 賽諾菲阿凡提斯德意志有限公司 | 藥物輸送裝置(四) |
KR20180011313A (ko) | 2015-06-04 | 2018-01-31 | 바스프 에스이 | 정제 화학약품의 개선된 제조를 위한 재조합 미생물 |
EP3384002A1 (en) | 2015-12-02 | 2018-10-10 | Basf Se | Method of producing proteins in filamentous fungi with decreased clr2 activity |
BR112018010310A2 (pt) | 2015-12-02 | 2018-12-04 | Basf Se | método de produção de polipeptídeos recombinantes, fungo filamentoso, uso de construção de ácido nucleico e uso de fungo filamentoso |
-
2016
- 2016-12-02 EP EP16808597.5A patent/EP3384002A1/en active Pending
- 2016-12-02 CA CA3004990A patent/CA3004990A1/en not_active Abandoned
- 2016-12-02 MX MX2018006535A patent/MX2018006535A/es unknown
- 2016-12-02 US US15/779,595 patent/US11299522B2/en active Active
- 2016-12-02 JP JP2018527754A patent/JP2019503168A/ja active Pending
- 2016-12-02 BR BR112018009729A patent/BR112018009729A8/pt not_active IP Right Cessation
- 2016-12-02 CN CN201680069639.2A patent/CN108291189A/zh active Pending
- 2016-12-02 WO PCT/EP2016/079526 patent/WO2017093450A1/en active Application Filing
- 2016-12-02 KR KR1020187017936A patent/KR20180084135A/ko not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
US11299522B2 (en) | 2022-04-12 |
KR20180084135A (ko) | 2018-07-24 |
MX2018006535A (es) | 2018-08-15 |
JP2019503168A (ja) | 2019-02-07 |
BR112018009729A8 (pt) | 2019-02-26 |
US20200308235A1 (en) | 2020-10-01 |
CN108291189A (zh) | 2018-07-17 |
EP3384002A1 (en) | 2018-10-10 |
WO2017093450A1 (en) | 2017-06-08 |
CA3004990A1 (en) | 2017-06-08 |
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Legal Events
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B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
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B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2699 DE 27-09-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |