BR112017020561A2 - transaminase mutante, proteína, ácido nucleico, célula hospedeira, método de produção de amina e uso de transaminase mutante - Google Patents

transaminase mutante, proteína, ácido nucleico, célula hospedeira, método de produção de amina e uso de transaminase mutante

Info

Publication number
BR112017020561A2
BR112017020561A2 BR112017020561A BR112017020561A BR112017020561A2 BR 112017020561 A2 BR112017020561 A2 BR 112017020561A2 BR 112017020561 A BR112017020561 A BR 112017020561A BR 112017020561 A BR112017020561 A BR 112017020561A BR 112017020561 A2 BR112017020561 A2 BR 112017020561A2
Authority
BR
Brazil
Prior art keywords
transaminase
mutant transaminase
mutant
protein
host cell
Prior art date
Application number
BR112017020561A
Other languages
English (en)
Other versions
BR112017020561B1 (pt
Inventor
Wirz Beat
Iding Hans
Pavlidis Ioannis
Steffen Weiss Martin
Spurr Paul
Paul Hanlon Steven
Bornscheuer Uwe
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR112017020561A2 publication Critical patent/BR112017020561A2/pt
Publication of BR112017020561B1 publication Critical patent/BR112017020561B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Abstract

a presente invenção refere-se a transaminase mutante com atividade de transaminase aprimorada com relação à transaminase do tipo selvagem, proteína de fusão que compreende a transaminase, polinucleotídeo que codifica a transaminase, célula hospedeira que compreende o polinucleotídeo, transaminase mutante e/ou proteína de fusão, método de produção de amina com a transaminase mutante ou proteína de fusão e uso da transaminase mutante ou proteína de fusão para a produção de amina.
BR112017020561-0A 2015-04-16 2016-04-13 Transaminase mutante, proteína de fusão, método de produção de amina e uso da transaminase mutante BR112017020561B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15001113 2015-04-16
EP15001113.8 2015-04-16
PCT/EP2016/058051 WO2016166120A1 (en) 2015-04-16 2016-04-13 Mutant transaminases as well as methods and uses relating thereto

Publications (2)

Publication Number Publication Date
BR112017020561A2 true BR112017020561A2 (pt) 2018-07-17
BR112017020561B1 BR112017020561B1 (pt) 2024-01-16

Family

ID=52997803

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017020561-0A BR112017020561B1 (pt) 2015-04-16 2016-04-13 Transaminase mutante, proteína de fusão, método de produção de amina e uso da transaminase mutante

Country Status (13)

Country Link
US (1) US10017749B2 (pt)
EP (1) EP3283622B1 (pt)
JP (1) JP6782708B2 (pt)
KR (1) KR102593068B1 (pt)
CN (1) CN107466322B (pt)
AU (1) AU2016249780B2 (pt)
BR (1) BR112017020561B1 (pt)
CA (1) CA2980407C (pt)
ES (1) ES2744623T3 (pt)
IL (1) IL254708B (pt)
MX (1) MX2017013136A (pt)
PL (1) PL3283622T3 (pt)
WO (1) WO2016166120A1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016166120A1 (en) * 2015-04-16 2016-10-20 F. Hoffmann-La Roche Ag Mutant transaminases as well as methods and uses relating thereto
US11359183B2 (en) 2017-06-14 2022-06-14 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
EP3450566A1 (en) * 2017-09-01 2019-03-06 Vito NV Method for producing chiral amines
JP7022819B2 (ja) * 2017-11-06 2022-02-18 アシムケム ライフ サイエンス （ティエンジン） カンパニー リミテッド トランスアミナーゼ突然変異体及びその使用
CN109957554B (zh) * 2017-12-26 2021-05-07 宁波酶赛生物工程有限公司 工程化转氨酶多肽及其应用
CN109370998B (zh) * 2018-11-30 2020-08-04 江南大学 一种催化效率提高的ω-转氨酶突变体I215F
CN109468297B (zh) * 2018-11-30 2020-11-03 江南大学 一种能够催化西他沙星五元环中间体的ω-转氨酶突变体
CN110904066B (zh) * 2019-12-18 2021-08-27 浙江工业大学 重组r型转氨酶、突变体及其应用
CN111363732B (zh) * 2020-03-12 2023-05-23 卡柔恩赛生物技术湖北有限公司 来源于土曲霉菌nih2624的转氨酶突变体及其应用
CN113373126B (zh) * 2021-06-17 2022-08-23 天津法莫西生物医药科技有限公司 一种转氨酶突变体及其编码基因和应用
WO2023012819A1 (en) * 2021-07-31 2023-02-09 Quantumzyme Llp Recombinant transaminase polypeptides
CN115975969A (zh) * 2021-10-14 2023-04-18 弈柯莱生物科技(上海)股份有限公司 转氨酶及用于制备西他列汀或其中间体的用途
CN115873819B (zh) * 2023-01-05 2023-05-02 山东省计算中心（国家超级计算济南中心） 基于超级计算辅助获得d-氨基酸转氨酶突变体及其应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2448816T3 (es) * 2009-02-26 2014-03-17 Codexis, Inc. Biocatalizadores de transaminasa
EP2593556B1 (en) * 2010-07-14 2017-08-30 DPx Holdings B.V. Enzymatic method for (r)-selective amination
KR101525549B1 (ko) * 2013-07-12 2015-06-04 연세대학교 산학협력단 오메가 트랜스아미나제의 촉매 작용을 이용한 비천연 아미노산의 제조방법
ES2734579T3 (es) * 2013-11-26 2019-12-10 Asymchem Lab Tianjin Co Ltd Transaminasa y utilización de la misma
KR20160089492A (ko) * 2013-11-26 2016-07-27 아심켐 래보러토리즈 (톈진) 컴퍼니, 리미티드 R형 ω-트랜스아미나제 및 그 응용
WO2016166120A1 (en) * 2015-04-16 2016-10-20 F. Hoffmann-La Roche Ag Mutant transaminases as well as methods and uses relating thereto

Also Published As

Publication number Publication date
JP2018511340A (ja) 2018-04-26
EP3283622A1 (en) 2018-02-21
US20160304843A1 (en) 2016-10-20
IL254708A0 (en) 2017-11-30
KR20170137103A (ko) 2017-12-12
ES2744623T3 (es) 2020-02-25
AU2016249780B2 (en) 2021-07-22
BR112017020561B1 (pt) 2024-01-16
CN107466322B (zh) 2022-04-15
WO2016166120A1 (en) 2016-10-20
MX2017013136A (es) 2018-02-21
CA2980407C (en) 2023-10-03
JP6782708B2 (ja) 2020-11-11
US10017749B2 (en) 2018-07-10
PL3283622T3 (pl) 2019-11-29
AU2016249780A1 (en) 2017-10-19
KR102593068B1 (ko) 2023-10-23
IL254708B (en) 2019-10-31
EP3283622B1 (en) 2019-07-03
AU2016249780A9 (en) 2021-05-27
CN107466322A (zh) 2017-12-12
CA2980407A1 (en) 2016-10-20

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Legal Events

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/04/2016, OBSERVADAS AS CONDICOES LEGAIS