BR112018009729A8 - método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico - Google Patents
método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleicoInfo
- Publication number
- BR112018009729A8 BR112018009729A8 BR112018009729A BR112018009729A BR112018009729A8 BR 112018009729 A8 BR112018009729 A8 BR 112018009729A8 BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A BR112018009729 A BR 112018009729A BR 112018009729 A8 BR112018009729 A8 BR 112018009729A8
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant polypeptide
- filamentous fungus
- producing
- nucleic acid
- myceliophthora thermophila
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/2488—Mannanases
- C12N9/2491—Beta-mannosidase (3.2.1.25), i.e. mannanase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
- C12Y301/03008—3-Phytase (3.1.3.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01025—Beta-mannosidase (3.2.1.25), i.e. mannanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21063—Oryzin (3.4.21.63)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
a presente invenção diz respeito a um método de produção de um polipeptídeo recombinante em um fungo filamentoso que é geneticamente modificado para diminuir ou eliminar a atividade do regulador da celulase 2 (clr2) e para expressar o referido polipeptídeo recombinante. o método diz respeito ainda a um fungo filamentoso myceliophthora thermophila que é geneticamente modificado para diminuir ou eliminar a atividade do clr2 e ao uso deste fungo filamentoso na produção de um polipeptídeo recombinante.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15197497 | 2015-12-02 | ||
PCT/EP2016/079526 WO2017093450A1 (en) | 2015-12-02 | 2016-12-02 | Method of producing proteins in filamentous fungi with decreased clr2 activity |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112018009729A2 BR112018009729A2 (pt) | 2018-11-21 |
BR112018009729A8 true BR112018009729A8 (pt) | 2019-02-26 |
Family
ID=54838169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112018009729A BR112018009729A8 (pt) | 2015-12-02 | 2016-12-02 | método para produzir um polipeptídeo recombinante, fungo filamentoso myceliophthora thermophila e uso de uma construção de ácido nucleico |
Country Status (9)
Country | Link |
---|---|
US (1) | US11299522B2 (pt) |
EP (1) | EP3384002A1 (pt) |
JP (1) | JP2019503168A (pt) |
KR (1) | KR20180084135A (pt) |
CN (1) | CN108291189A (pt) |
BR (1) | BR112018009729A8 (pt) |
CA (1) | CA3004990A1 (pt) |
MX (1) | MX2018006535A (pt) |
WO (1) | WO2017093450A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20180084135A (ko) | 2015-12-02 | 2018-07-24 | 바스프 에스이 | 감소된 clr2 활성을 갖는 사상 진균에서 단백질을 생산하는 방법 |
CN108291191A (zh) | 2015-12-02 | 2018-07-17 | 巴斯夫欧洲公司 | 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法 |
CA3073646A1 (en) | 2017-08-21 | 2019-02-28 | Dyadic International Inc. | Production of flu vaccine in myceliophthora thermophila |
CN110042116A (zh) * | 2019-04-30 | 2019-07-23 | 贵州大学 | 一种稻瘟菌生物钟控制基因MoCCG4的重组载体和表达方法 |
CN112813091A (zh) * | 2021-02-03 | 2021-05-18 | 山东农业大学 | Nat1基因作为筛选标记在卵菌遗传转化中的应用 |
CN113151264A (zh) * | 2021-03-11 | 2021-07-23 | 上海中溶科技有限公司 | 一种利用正向调控基因构建高产纤维素酶菌株的方法及应用 |
WO2024107812A1 (en) | 2022-11-15 | 2024-05-23 | Basf Corporation | Methods of reducing conidiation in cell culture |
Family Cites Families (28)
Publication number | Priority date | Publication date | Assignee | Title |
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EP0635574B1 (en) | 1993-07-23 | 2003-04-23 | Dsm N.V. | Selection marker gene free recombinant strains, a method for obtaining them and the use of these strains |
WO1998046772A2 (en) | 1997-04-11 | 1998-10-22 | Dsm N.V. | Gene conversion as a tool for the construction of recombinant industrial filamentous fungi |
KR100618495B1 (ko) | 1998-10-06 | 2006-08-31 | 마크 아론 에말파브 | 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 |
EP2102366A4 (en) | 2006-12-10 | 2010-01-27 | Dyadic International Inc | EXPRESSION AND HIGH-DROP SCREENING OF COMPLEX EXPRESSED DNA LIBRARIES IN FADENED MUSHROOMS |
WO2010107303A2 (en) * | 2009-03-16 | 2010-09-23 | Dyadic Nederland B.V. | New fungal production system |
US20120005812A1 (en) | 2010-07-06 | 2012-01-12 | Paul Corzatt | Insect protective garment |
WO2012048334A2 (en) | 2010-10-08 | 2012-04-12 | Dyadic International (Usa) Inc. | Novel fungal proteases |
WO2013022594A1 (en) | 2011-07-21 | 2013-02-14 | The Regents Of The University Of California | Transcription factors for the production of cellulose degrading enzymes |
WO2013048661A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
WO2014202620A2 (en) * | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
CN105683378B (zh) | 2013-09-25 | 2021-06-29 | 巴斯夫欧洲公司 | 用于精细化学品的改进生产的重组微生物 |
KR102416059B1 (ko) | 2013-12-09 | 2022-07-01 | 바스프 에스이 | 에레모테시움의 발효를 통한 리보플라빈의 더 높은 생성을 위한 베타-산화 경로의 효소를 엔코딩하는 유전자 및 지방산 수송체 유전자의 과-발현 |
CN113481217A (zh) | 2013-12-13 | 2021-10-08 | 巴斯夫欧洲公司 | 用于精细化学品的改进生产的重组微生物 |
WO2015092576A1 (en) | 2013-12-17 | 2015-06-25 | Basf Se | Improved process for the production of gamma-aminobutyric acid (gaba) |
WO2015092599A1 (en) | 2013-12-17 | 2015-06-25 | Basf Se | Improved process for the production of gamma-aminobutyric acid (gaba) |
BR112016018195A2 (pt) | 2014-02-07 | 2018-02-20 | Basf Se | micro-organismo modificado, método de produção de um composto orgânico e uso de um micro-organismo modificado |
BR112016018193B1 (pt) | 2014-02-07 | 2023-04-11 | Basf Se | Micro-organismo modificado, método de produção de ácido succínico e uso de um micro-organismo modificado |
MY191429A (en) | 2014-02-07 | 2022-06-27 | Basf Se | Modified microorganism with improved biomass separation behaviour |
WO2015135980A1 (en) | 2014-03-11 | 2015-09-17 | Basf Se | The use of polymers to improve the rheology of base suspensions during fermentation |
CA2942990A1 (en) | 2014-03-19 | 2015-09-24 | Basf Se | The use of glycerol with limited feed of carbohydrates for fermentation |
KR102308323B1 (ko) | 2014-05-08 | 2021-10-06 | 바스프 에스이 | 수크로스 상의 정밀 화학물질의 개선된 생산을 위한 유전자 변형된 미생물 |
JP2017515494A (ja) | 2014-05-21 | 2017-06-15 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | コルネキシスチン及びヒドロキシコルネキシスチンの生合成のための遺伝子クラスター |
WO2016030373A1 (en) | 2014-08-28 | 2016-03-03 | Basf Se | Modified microorganism for improved production of fine chemicals on sucrose |
WO2016091980A1 (en) | 2014-12-10 | 2016-06-16 | Basf Se | Method of removing dna from biotechnological products |
TW201707740A (zh) | 2015-06-03 | 2017-03-01 | 賽諾菲阿凡提斯德意志有限公司 | 藥物輸送裝置(四) |
KR20180011313A (ko) | 2015-06-04 | 2018-01-31 | 바스프 에스이 | 정제 화학약품의 개선된 제조를 위한 재조합 미생물 |
KR20180084135A (ko) | 2015-12-02 | 2018-07-24 | 바스프 에스이 | 감소된 clr2 활성을 갖는 사상 진균에서 단백질을 생산하는 방법 |
CN108291191A (zh) | 2015-12-02 | 2018-07-17 | 巴斯夫欧洲公司 | 在具有降低的clr1活性的丝状真菌中生产蛋白质的方法 |
-
2016
- 2016-12-02 KR KR1020187017936A patent/KR20180084135A/ko not_active Application Discontinuation
- 2016-12-02 EP EP16808597.5A patent/EP3384002A1/en active Pending
- 2016-12-02 US US15/779,595 patent/US11299522B2/en active Active
- 2016-12-02 CN CN201680069639.2A patent/CN108291189A/zh active Pending
- 2016-12-02 CA CA3004990A patent/CA3004990A1/en not_active Abandoned
- 2016-12-02 MX MX2018006535A patent/MX2018006535A/es unknown
- 2016-12-02 JP JP2018527754A patent/JP2019503168A/ja active Pending
- 2016-12-02 BR BR112018009729A patent/BR112018009729A8/pt not_active IP Right Cessation
- 2016-12-02 WO PCT/EP2016/079526 patent/WO2017093450A1/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
KR20180084135A (ko) | 2018-07-24 |
MX2018006535A (es) | 2018-08-15 |
CA3004990A1 (en) | 2017-06-08 |
WO2017093450A1 (en) | 2017-06-08 |
US20200308235A1 (en) | 2020-10-01 |
JP2019503168A (ja) | 2019-02-07 |
BR112018009729A2 (pt) | 2018-11-21 |
EP3384002A1 (en) | 2018-10-10 |
US11299522B2 (en) | 2022-04-12 |
CN108291189A (zh) | 2018-07-17 |
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