BR112021022223B1 - Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina - Google Patents
Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina Download PDFInfo
- Publication number
- BR112021022223B1 BR112021022223B1 BR112021022223-5A BR112021022223A BR112021022223B1 BR 112021022223 B1 BR112021022223 B1 BR 112021022223B1 BR 112021022223 A BR112021022223 A BR 112021022223A BR 112021022223 B1 BR112021022223 B1 BR 112021022223B1
- Authority
- BR
- Brazil
- Prior art keywords
- amino acid
- glutamine
- polypeptide
- microorganism
- seq
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2020-0027322 | 2020-03-04 | ||
| KR1020200027322A KR102198072B1 (ko) | 2020-03-04 | 2020-03-04 | 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법 |
| PCT/KR2021/002652 WO2021177731A1 (ko) | 2020-03-04 | 2021-03-04 | 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112021022223A2 BR112021022223A2 (pt) | 2022-09-20 |
| BR112021022223B1 true BR112021022223B1 (pt) | 2023-09-26 |
Family
ID=74127205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112021022223-5A BR112021022223B1 (pt) | 2020-03-04 | 2021-03-04 | Polipeptídeo modificado, polinucleotídeo, micro-organismo e método para produzir l-glutamina |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US12270061B2 (https=) |
| EP (1) | EP3954767A4 (https=) |
| JP (2) | JP7371119B2 (https=) |
| KR (1) | KR102198072B1 (https=) |
| CN (1) | CN114375332B (https=) |
| BR (1) | BR112021022223B1 (https=) |
| WO (1) | WO2021177731A1 (https=) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102198072B1 (ko) | 2020-03-04 | 2021-01-04 | 씨제이제일제당 주식회사 | 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법 |
| WO2022108383A1 (ko) | 2020-11-20 | 2022-05-27 | 씨제이제일제당 (주) | L-글루타민 생산능이 향상된 미생물 및 이를 이용한 l-글루타민 생산 방법 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060269975A1 (en) * | 1999-07-16 | 2006-11-30 | Basf Aktiengesellschaft | Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis |
| JP4560998B2 (ja) | 2001-02-05 | 2010-10-13 | 味の素株式会社 | 発酵法によるｌ−グルタミンの製造法及びｌ−グルタミン生産菌 |
| RU2230114C2 (ru) * | 2001-11-30 | 2004-06-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Мутантная глутаминсинтетаза, фрагмент днк, штамм escherichia coli - продуцент l-глутамина и способ получения l-аминокислот |
| JP2004187684A (ja) * | 2002-11-26 | 2004-07-08 | Ajinomoto Co Inc | Ｌ−グルタミンの製造法及びｌ−グルタミン生産菌 |
| JP4576850B2 (ja) | 2003-03-03 | 2010-11-10 | 味の素株式会社 | 発酵法によるｌ−アルギニン又はｌ−リジンの製造法 |
| EP1460128B1 (en) | 2003-03-03 | 2016-11-30 | Ajinomoto Co., Inc. | Method for producing L-arginine or L-lysine by fermentation |
| KR100620092B1 (ko) | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| CN100392075C (zh) | 2006-06-29 | 2008-06-04 | 清华大学 | 谷氨酰胺合成酶及其专用表达工程菌与应用 |
| JP5048996B2 (ja) | 2006-11-10 | 2012-10-17 | 昭和電工株式会社 | 加工性に優れた耐摩耗性アルミニウム合金材およびその製造方法 |
| PT2814965T (pt) * | 2012-02-16 | 2018-05-23 | Syngenta Participations Ag | Proteínas pesticidas manipuladas |
| JP2015513406A (ja) * | 2012-03-12 | 2015-05-14 | ハンファ ケミカル コーポレーション | 改変グルタミン合成酵素をコードするポリヌクレオチドを含む発現ベクターおよびこれを用いた標的タンパク質の製造方法 |
| MY179341A (en) * | 2014-04-30 | 2020-11-04 | Evonik Operations Gmbh | Method for producing l-amino acids using an alkaliphilic bacteria |
| KR101632642B1 (ko) | 2015-01-29 | 2016-06-22 | 씨제이제일제당 주식회사 | 신규한 프로모터 및 그의 용도 |
| MY186296A (en) | 2016-08-31 | 2021-07-06 | Cj Cheiljedang Corp | Novel promoter and use thereof |
| KR102198072B1 (ko) | 2020-03-04 | 2021-01-04 | 씨제이제일제당 주식회사 | 글루타민 신테타아제 변이형 폴리펩티드 및 이를 이용한 l-글루타민 생산 방법 |
-
2020
- 2020-03-04 KR KR1020200027322A patent/KR102198072B1/ko active Active
-
2021
- 2021-03-04 JP JP2021562185A patent/JP7371119B2/ja active Active
- 2021-03-04 US US17/604,896 patent/US12270061B2/en active Active
- 2021-03-04 WO PCT/KR2021/002652 patent/WO2021177731A1/ko not_active Ceased
- 2021-03-04 BR BR112021022223-5A patent/BR112021022223B1/pt active IP Right Grant
- 2021-03-04 EP EP21764687.6A patent/EP3954767A4/en active Pending
- 2021-03-04 CN CN202180004868.7A patent/CN114375332B/zh active Active
-
2023
- 2023-05-31 JP JP2023090522A patent/JP2023110008A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022529297A (ja) | 2022-06-20 |
| CN114375332B (zh) | 2024-01-12 |
| WO2021177731A1 (ko) | 2021-09-10 |
| CN114375332A (zh) | 2022-04-19 |
| JP2023110008A (ja) | 2023-08-08 |
| EP3954767A1 (en) | 2022-02-16 |
| US12270061B2 (en) | 2025-04-08 |
| JP7371119B2 (ja) | 2023-10-30 |
| BR112021022223A2 (pt) | 2022-09-20 |
| KR102198072B1 (ko) | 2021-01-04 |
| US20220396815A1 (en) | 2022-12-15 |
| EP3954767A4 (en) | 2022-08-03 |
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| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
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| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
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