JP7237586B2 - 修飾ナノポア、それを含む組成物およびその使用 - Google Patents

修飾ナノポア、それを含む組成物およびその使用 Download PDF

Info

Publication number
JP7237586B2
JP7237586B2 JP2018530754A JP2018530754A JP7237586B2 JP 7237586 B2 JP7237586 B2 JP 7237586B2 JP 2018530754 A JP2018530754 A JP 2018530754A JP 2018530754 A JP2018530754 A JP 2018530754A JP 7237586 B2 JP7237586 B2 JP 7237586B2
Authority
JP
Japan
Prior art keywords
nanopore
amino acid
clya
dna
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018530754A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019507583A (ja
JP2019507583A5 (enExample
Inventor
マグリア，ジオバンニ
フランチェスキニ，ロレンゾ
ブラウンス，タイン
ジョン ヘロン，アンドリュー
ニシャンタ ジャヤシンゲ，ラクマル
ジェーン ウォーレス，エリザベス
Original Assignee
カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント filed Critical カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント
Publication of JP2019507583A publication Critical patent/JP2019507583A/ja
Publication of JP2019507583A5 publication Critical patent/JP2019507583A5/ja
Priority to JP2021198939A priority Critical patent/JP2022046503A/ja
Application granted granted Critical
Publication of JP7237586B2 publication Critical patent/JP7237586B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2018530754A 2015-12-08 2016-12-08 修飾ナノポア、それを含む組成物およびその使用 Active JP7237586B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2021198939A JP2022046503A (ja) 2015-12-08 2021-12-08 修飾ナノポア、それを含む組成物およびその使用

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562264709P 2015-12-08 2015-12-08
US62/264,709 2015-12-08
PCT/IB2016/001841 WO2017098322A1 (en) 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2021198939A Division JP2022046503A (ja) 2015-12-08 2021-12-08 修飾ナノポア、それを含む組成物およびその使用

Publications (3)

Publication Number Publication Date
JP2019507583A JP2019507583A (ja) 2019-03-22
JP2019507583A5 JP2019507583A5 (enExample) 2020-01-30
JP7237586B2 true JP7237586B2 (ja) 2023-03-13

Family

ID=58098655

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2018530754A Active JP7237586B2 (ja) 2015-12-08 2016-12-08 修飾ナノポア、それを含む組成物およびその使用
JP2021198939A Pending JP2022046503A (ja) 2015-12-08 2021-12-08 修飾ナノポア、それを含む組成物およびその使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2021198939A Pending JP2022046503A (ja) 2015-12-08 2021-12-08 修飾ナノポア、それを含む組成物およびその使用

Country Status (8)

Country Link
US (1) US10976300B2 (enExample)
EP (1) EP3387432B1 (enExample)
JP (2) JP7237586B2 (enExample)
KR (1) KR102837999B1 (enExample)
CN (1) CN109072295B (enExample)
AU (1) AU2016369071B2 (enExample)
CA (1) CA3011830C (enExample)
WO (1) WO2017098322A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
EP3137627B1 (en) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
EP4122949B1 (en) 2016-04-06 2024-06-05 Oxford Nanopore Technologies plc Mutant pore
US11531020B2 (en) * 2017-01-18 2022-12-20 Youhealth Biotech, Limited Methods and compositions for nanopore sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
WO2020010589A1 (en) * 2018-07-12 2020-01-16 Shenzhen Genorivision Technology Co., Ltd. An apparatus for biopolymer sequencing
WO2020241752A1 (en) 2019-05-28 2020-12-03 The University Of Tokyo Analyzing apparatus and method using a pore device
AU2020389020A1 (en) * 2019-11-19 2022-06-09 Rijksuniversiteit Groningen Artificial nanopores and uses and methods relating thereto
EP4070092B1 (en) * 2019-12-02 2023-08-30 Oxford Nanopore Technologies PLC Method of characterising a target polypeptide using a nanopore
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN115398008B (zh) * 2020-04-14 2025-05-09 成都今是科技有限公司 纳米孔制备和检测方法及其检测装置
US20230184767A1 (en) * 2020-05-20 2023-06-15 The Board Of Trustees Of The University Of Illinois Methods and compositions for rapid direct detection and differentiation of infectious from noninfectious virus
US20230295712A1 (en) * 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
CN112179955B (zh) * 2020-09-27 2024-05-10 西北工业大学 基于聚精氨酸和聚谷氨酸逐层修饰的纳米孔制备pH响应的纳米流体二极管方法
KR102309424B1 (ko) * 2020-09-28 2021-10-07 한국생명공학연구원 나노포어를 이용한 리보스위치-표적 물질의 스크리닝 방법
CN117255945A (zh) 2021-03-31 2023-12-19 伊鲁米纳公司 纳米孔传感器装置
GB2610380A (en) 2021-08-23 2023-03-08 Cambridge Entpr Ltd Nucleic acid detection
GB202113935D0 (en) 2021-09-29 2021-11-10 Cambridge Entpr Ltd Nucleic acid characterisation
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
CN114768530B (zh) * 2022-04-29 2023-03-28 中国工程物理研究院材料研究所 二硫化钼在电解分离氢同位素中的应用
CN120435657A (zh) 2022-10-28 2025-08-05 格罗宁根大学 蛋白质的基于纳米孔的分析
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
WO2026052817A1 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method
WO2026052811A2 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014153625A1 (en) 2013-03-25 2014-10-02 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
AU5631200A (en) 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
EP1208207A2 (de) 1999-08-31 2002-05-29 Michael Niederweis Verfahren zur herstellung eines kanalbildenden proteins
EP2083015B1 (en) 2000-02-11 2016-04-06 The Texas A & M University System Biosensor compositions and methods of use
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
EP2194123B1 (en) 2003-03-25 2012-08-22 Stratagene California DNA polymerase fusions and uses thereof
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
JP2005253427A (ja) 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
WO2007084103A2 (en) 2004-12-21 2007-07-26 The Texas A & M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP1963530B1 (en) 2005-12-22 2011-07-27 Pacific Biosciences of California, Inc. Active surface coupled polymerases
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
US8698481B2 (en) 2007-09-12 2014-04-15 President And Fellows Of Harvard College High-resolution molecular sensor
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
EP3269824A1 (en) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009143425A1 (en) 2008-05-22 2009-11-26 The Regents Of The University Of California Membrane precursors and membranes formed therefrom
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
EP2307540B1 (en) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20100148126A1 (en) 2008-11-26 2010-06-17 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010122293A1 (en) 2009-04-20 2010-10-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
WO2011106456A2 (en) 2010-02-23 2011-09-01 University Of Washington Artificial mycolic acid membranes
US9073990B2 (en) * 2010-04-05 2015-07-07 Bar-Llan University Protease-activatable pore-forming polypeptides
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
EP2673638B1 (en) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutant pores
EP3633370B1 (en) 2011-05-27 2024-05-01 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
US9580480B2 (en) 2011-05-31 2017-02-28 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
IN2014DN00221A (enExample) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
CA3113287C (en) 2011-09-23 2022-12-20 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (en) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013109970A1 (en) 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
EP2815235B1 (en) 2012-02-15 2020-04-15 Oxford Nanopore Technologies Limited Aptamer method
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
TWI655213B (zh) 2012-07-13 2019-04-01 Menicon Co., Ltd. 自我組織化肽衍生物的製造方法
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
CA2889664C (en) 2012-10-26 2020-12-29 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2954320B1 (en) 2013-02-07 2018-04-18 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. Hybrid nanopores and uses thereof for detection of analytes
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
WO2014153047A1 (en) 2013-03-14 2014-09-25 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
WO2015051378A1 (en) * 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
CN117947149A (zh) 2013-10-18 2024-04-30 牛津纳米孔科技公开有限公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
EP3137627B1 (en) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014153625A1 (en) 2013-03-25 2014-10-02 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ＦＲＡＮＣＥＳＣＨＩＮＩ， Ｌｏｒｅｎｚｏ ｅｔ ａｌ．，Ａ ＮＡＮＯＰＯＲＥ ＭＡＣＨＩＮＥ ＰＲＯＭＯＴＥＳ ＴＨＥ ＶＥＣＴＯＲＩＡＬ ＴＲＡＮＳＰＯＲＴ ＯＦ ＤＮＡ ＡＣＲＯＳＳ ＭＥＭＢＲＡＮＥＳ，ＮＡＴＵＲＥ ＣＯＭＭＵＮＩＣＡＴＩＯＮＳ，ＭＡＣＭＩＬＬＡＮ ＰＵＢＬＩＳＨＥＲＳ ＬＩＭＩＴＥＤ，２０１３年 ９月，Ｖｏｌ． ４， ２４１５，ｐｐ． １－８，ＵＲＬ，ｈｔｔｐｓ：／／ｗｗｗ．ｎａｔｕｒｅ．ｃｏｍ／ａｒｔｉｃｌｅｓ／ｎｃｏｍｍｓ３４１５

Also Published As

Publication number Publication date
US20180364214A1 (en) 2018-12-20
JP2019507583A (ja) 2019-03-22
WO2017098322A1 (en) 2017-06-15
EP3387432B1 (en) 2022-09-28
KR20180089499A (ko) 2018-08-08
CA3011830A1 (en) 2017-06-15
CA3011830C (en) 2024-11-12
EP3387432A1 (en) 2018-10-17
AU2016369071A1 (en) 2018-06-28
AU2016369071B2 (en) 2022-05-19
US10976300B2 (en) 2021-04-13
CN109072295A (zh) 2018-12-21
JP2022046503A (ja) 2022-03-23
CN109072295B (zh) 2022-05-31
KR102837999B1 (ko) 2025-07-24

Similar Documents

Publication Publication Date Title
JP2022046503A (ja) 修飾ナノポア、それを含む組成物およびその使用
EP3137490B1 (en) Mutant pores
KR102086182B1 (ko) 효소 방법
JP2024012307A (ja) 修飾ナノポア、それを含む組成物、およびそれらの使用
JP6226869B2 (ja) 酵素法
US9562887B2 (en) Methods of enhancing translocation of charged analytes through transmembrane protein pores
EP3259281B1 (en) Hetero-pores
KR102429381B1 (ko) 분석물을 막횡단 세공에 전달하는 방법
KR101963918B1 (ko) 커플링 방법
EP3204511B1 (en) Mutant pores
CN115698331A (zh) 使用检测器选择性地表征多核苷酸的方法
US20250137046A1 (en) Pore
CN115747211A (zh) 一种纳米孔测序用测序接头的设计及应用
EP3404113A1 (en) Method for detecting protein-dna interaction
EP4362028A1 (en) Mutant aerolysin and uses thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180809

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181025

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191209

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20191209

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201208

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210303

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20210810

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211208

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20211208

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20211216

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20211221

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20220114

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20220118

C22 Notice of designation (change) of administrative judge

Free format text: JAPANESE INTERMEDIATE CODE: C22

Effective date: 20221213

C23 Notice of termination of proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C23

Effective date: 20230124

C03 Trial/appeal decision taken

Free format text: JAPANESE INTERMEDIATE CODE: C03

Effective date: 20230221

C30A Notification sent

Free format text: JAPANESE INTERMEDIATE CODE: C3012

Effective date: 20230221

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20230301

R150 Certificate of patent or registration of utility model

Ref document number: 7237586

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250