CA3011830C - Modified nanopores, compositions comprising the same, and uses thereof - Google Patents

Modified nanopores, compositions comprising the same, and uses thereof

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Publication number
CA3011830C
CA3011830C CA3011830A CA3011830A CA3011830C CA 3011830 C CA3011830 C CA 3011830C CA 3011830 A CA3011830 A CA 3011830A CA 3011830 A CA3011830 A CA 3011830A CA 3011830 C CA3011830 C CA 3011830C
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CA
Canada
Prior art keywords
clya
modified
mutant forms
compositions containing
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3011830A
Other languages
English (en)
French (fr)
Other versions
CA3011830A1 (en
Inventor
Giovanni Maglia
Lorenzo Franceschini
Tine Brouns
Andrew John Heron
Lakmal Nishantha Jayasinghe
Elizabeth Jayne Wallace
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Katholieke Universiteit Leuven
Original Assignee
Katholieke Universiteit Leuven
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Publication date
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Publication of CA3011830A1 publication Critical patent/CA3011830A1/en
Application granted granted Critical
Publication of CA3011830C publication Critical patent/CA3011830C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3011830A 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof Active CA3011830C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562264709P 2015-12-08 2015-12-08
US62/264,709 2015-12-08
PCT/IB2016/001841 WO2017098322A1 (en) 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof

Publications (2)

Publication Number Publication Date
CA3011830A1 CA3011830A1 (en) 2017-06-15
CA3011830C true CA3011830C (en) 2024-11-12

Family

ID=58098655

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3011830A Active CA3011830C (en) 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof

Country Status (8)

Country Link
US (1) US10976300B2 (enExample)
EP (1) EP3387432B1 (enExample)
JP (2) JP7237586B2 (enExample)
KR (1) KR102837999B1 (enExample)
CN (1) CN109072295B (enExample)
AU (1) AU2016369071B2 (enExample)
CA (1) CA3011830C (enExample)
WO (1) WO2017098322A1 (enExample)

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Also Published As

Publication number Publication date
JP2022046503A (ja) 2022-03-23
JP2019507583A (ja) 2019-03-22
CA3011830A1 (en) 2017-06-15
AU2016369071B2 (en) 2022-05-19
EP3387432B1 (en) 2022-09-28
AU2016369071A1 (en) 2018-06-28
WO2017098322A1 (en) 2017-06-15
CN109072295A (zh) 2018-12-21
CN109072295B (zh) 2022-05-31
US20180364214A1 (en) 2018-12-20
EP3387432A1 (en) 2018-10-17
KR102837999B1 (ko) 2025-07-24
KR20180089499A (ko) 2018-08-08
US10976300B2 (en) 2021-04-13
JP7237586B2 (ja) 2023-03-13

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