JP6310394B2 - 抗体を精製する方法 - Google Patents

抗体を精製する方法 Download PDF

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Publication number
JP6310394B2
JP6310394B2 JP2014535821A JP2014535821A JP6310394B2 JP 6310394 B2 JP6310394 B2 JP 6310394B2 JP 2014535821 A JP2014535821 A JP 2014535821A JP 2014535821 A JP2014535821 A JP 2014535821A JP 6310394 B2 JP6310394 B2 JP 6310394B2
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Prior art keywords
antibody
unnatural
amino acid
antibodies
heavy chain
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Expired - Fee Related
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Japanese (ja)
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JP2015500002A5 (enExample
JP2015500002A (ja
Inventor
バーネット，マシュー，ジェイ．
ムーア，グレゴリー，エル．
デスジャルレイス，ジョン
ラシッド，ルマナ
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ゼンコア インコーポレイテッド
ゼンコア インコーポレイテッド
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Publication of JP2015500002A5 publication Critical patent/JP2015500002A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/522—CH1 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/524—CH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/72—Increased effector function due to an Fc-modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2014535821A 2011-10-10 2012-10-10 抗体を精製する方法 Expired - Fee Related JP6310394B2 (ja)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161545498P 2011-10-10 2011-10-10
US61/545,498 2011-10-10
US201261593846P 2012-02-01 2012-02-01
US61/593,846 2012-02-01
US201261598686P 2012-02-14 2012-02-14
US61/598,686 2012-02-14
US201213568028A 2012-08-06 2012-08-06
US13/568,028 2012-08-06
PCT/US2012/059582 WO2013055809A1 (en) 2011-10-10 2012-10-10 A method for purifying antibodies

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018049804A Division JP6667562B2 (ja) 2011-10-10 2018-03-16 抗体を精製する方法

Publications (3)

Publication Number Publication Date
JP2015500002A JP2015500002A (ja) 2015-01-05
JP2015500002A5 JP2015500002A5 (enExample) 2015-12-03
JP6310394B2 true JP6310394B2 (ja) 2018-04-11

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Family Applications (3)

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JP2014535821A Expired - Fee Related JP6310394B2 (ja) 2011-10-10 2012-10-10 抗体を精製する方法
JP2018049804A Active JP6667562B2 (ja) 2011-10-10 2018-03-16 抗体を精製する方法
JP2020029547A Active JP7022162B2 (ja) 2011-10-10 2020-02-25 抗体を精製する方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2018049804A Active JP6667562B2 (ja) 2011-10-10 2018-03-16 抗体を精製する方法
JP2020029547A Active JP7022162B2 (ja) 2011-10-10 2020-02-25 抗体を精製する方法

Country Status (6)

Country Link
EP (2) EP2766392B1 (enExample)
JP (3) JP6310394B2 (enExample)
AU (4) AU2012323287B2 (enExample)
CA (2) CA3182462A1 (enExample)
DK (1) DK2766392T3 (enExample)
WO (1) WO2013055809A1 (enExample)

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