JP6265905B2 - 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ - Google Patents

普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ Download PDF

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JP6265905B2
JP6265905B2 JP2014540506A JP2014540506A JP6265905B2 JP 6265905 B2 JP6265905 B2 JP 6265905B2 JP 2014540506 A JP2014540506 A JP 2014540506A JP 2014540506 A JP2014540506 A JP 2014540506A JP 6265905 B2 JP6265905 B2 JP 6265905B2
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molecule
mediator
region
detection
probe
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JP2014533107A (ja
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ロート，ギュンター
ファルティン，ベルント
シュテッテン，フェリックス フォン
シュテッテン，フェリックス フォン
ワドル，シモン
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Albert Ludwigs Universitaet Freiburg
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Albert Ludwigs Universitaet Freiburg
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
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  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
JP2014540506A 2011-11-10 2012-11-12 普遍的リアルタイム多分析物検出用の二官能性オリゴヌクレオチドプローブ Active JP6265905B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102011055247.2 2011-11-10
DE102011055247A DE102011055247A1 (de) 2011-11-10 2011-11-10 Multianalyt-Reportersystem
PCT/EP2012/072402 WO2013079307A1 (de) 2011-11-10 2012-11-12 Bifunktionale oligonukleotidsonde zur universellen echtzeit multianalytdetektion

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JP2014533107A JP2014533107A (ja) 2014-12-11
JP6265905B2 true JP6265905B2 (ja) 2018-01-24

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US (1) US11293053B2 (enExample)
EP (1) EP2776585B8 (enExample)
JP (1) JP6265905B2 (enExample)
CN (1) CN103946397B (enExample)
CA (1) CA2854427A1 (enExample)
DE (1) DE102011055247A1 (enExample)
ES (1) ES2698903T3 (enExample)
IN (1) IN2014DN04645A (enExample)
WO (1) WO2013079307A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012100824A1 (de) 2012-02-01 2013-09-05 Albert-Ludwigs-Universität Freiburg Gemultiplexte Digital PCR
CA2904545A1 (en) * 2012-03-20 2013-09-26 Universite Laval A nucleic acid detection method comprising target specific indexing probes (tsip) comprising a releasable segment to be detected via fluorescence when bound to a capture probe
US20140272959A1 (en) * 2013-03-14 2014-09-18 President And Fellows Of Harvard College Methods of Hybridizing Probes to Genomic DNA
EP3011056B1 (en) * 2013-06-19 2019-03-06 Luminex Corporation Real-time multiplexed hydrolysis probe assay
EP3034619B1 (de) * 2014-12-16 2018-10-17 microBIOMix GmbH Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme
WO2016134191A1 (en) * 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
KR101875662B1 (ko) * 2015-12-01 2018-08-02 에스디 바이오센서 주식회사 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법
CN107024583B (zh) * 2016-01-29 2019-03-08 中国科学院苏州纳米技术与纳米仿生研究所 硫黄素t适配体、其筛选方法及应用
CA3049833A1 (en) * 2016-12-23 2018-06-28 Albert-Ludwigs-Universitat Freiburg Two-piece mediator probe
CN108823287B (zh) * 2017-04-28 2019-04-12 厦门大学 一种检测靶核酸序列的方法
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN111100935B (zh) * 2018-10-26 2023-03-31 厦门大学 一种细菌耐药基因检测的方法
EP3992302A1 (en) * 2020-10-29 2022-05-04 Biocartis NV Generic cartridge and method for multiplex nucleic acid detection
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
EP4265735A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheid-baren signalstäken
WO2023203227A1 (de) 2022-04-22 2023-10-26 Hahn-Schickard-Gesellschaft Für Angewandte Forschung E. V. Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheidbaren signalstärken
EP4265734A1 (de) 2022-04-22 2023-10-25 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4511509A1 (de) 2022-04-22 2025-02-26 Hahn-Schickard-Gesellschaft für angewandte Forschung e. V. Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes
EP4372100A1 (en) 2022-11-15 2024-05-22 Stilla Technologies Data analysis method for multiplex digital pcr using color-combination
WO2025026366A1 (en) * 2023-07-31 2025-02-06 Coyote Bioscience Co., Ltd. Compositions and methods for nucleic acid detection
CN117144067A (zh) * 2023-10-31 2023-12-01 中国医学科学院北京协和医院 用于多重化核酸检测的组合物和方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4751177A (en) * 1985-06-13 1988-06-14 Amgen Methods and kits for performing nucleic acid hybridization assays
US5846708A (en) 1991-11-19 1998-12-08 Massachusetts Institiute Of Technology Optical and electrical methods and apparatus for molecule detection
US6099803A (en) 1994-07-07 2000-08-08 Nanogen, Inc. Advanced active electronic devices for molecular biological analysis and diagnostics
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
GB9615516D0 (en) * 1996-07-24 1996-09-04 Jsd Tech Ltd Bacillus cereus
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
AU2849800A (en) 1999-01-15 2000-08-01 Ljl Biosystems, Inc. Methods and apparatus for detecting polynucleotide hybridization
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
DE60040335D1 (de) * 1999-07-14 2008-11-06 Packard Bioscience Co Derivatisierte nukleinsäuren und deren verwendung
US6380377B1 (en) * 2000-07-14 2002-04-30 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
CA2426824A1 (en) * 2000-10-24 2002-07-25 The Board Of Trustees Of The Leland Stanford Junior University Direct multiplex characterization of genomic dna
US20040014078A1 (en) * 2002-02-06 2004-01-22 James Xia Compositions and methods for rolling circle amplification
GB0230238D0 (en) * 2002-12-28 2003-02-05 Fu Guoliang Combined exponential and linear amplification
DE10316159A1 (de) 2003-04-09 2004-10-28 Klapproth, Holger, Dr. 5`-3`-Exonuklease Assay zur Bestimmung der Konzentration von Nukleinsäuren
JP5520449B2 (ja) * 2007-03-06 2014-06-11 株式会社ビー・エム・エル 核酸の検出方法
CN102301000B (zh) * 2008-03-15 2015-04-29 豪洛捷公司 核酸分子扩增反应的分析组分及方法
WO2012096430A1 (en) 2011-01-11 2012-07-19 Seegene, Inc. Detection of target nucleic acid sequences by pto cleavage and extension assay

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Publication number Publication date
CA2854427A1 (en) 2013-06-06
EP2776585B1 (de) 2018-08-22
US11293053B2 (en) 2022-04-05
EP2776585A1 (de) 2014-09-17
DE102011055247A1 (de) 2013-05-16
IN2014DN04645A (enExample) 2015-05-08
CN103946397A (zh) 2014-07-23
CN103946397B (zh) 2021-03-16
ES2698903T3 (es) 2019-02-06
JP2014533107A (ja) 2014-12-11
US20140315747A1 (en) 2014-10-23
WO2013079307A1 (de) 2013-06-06
EP2776585B8 (de) 2019-04-10

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