ATE409236T1 - Derivatisierte nukleinsäuren und deren verwendung - Google Patents

Derivatisierte nukleinsäuren und deren verwendung

Info

Publication number
ATE409236T1
ATE409236T1 AT00950341T AT00950341T ATE409236T1 AT E409236 T1 ATE409236 T1 AT E409236T1 AT 00950341 T AT00950341 T AT 00950341T AT 00950341 T AT00950341 T AT 00950341T AT E409236 T1 ATE409236 T1 AT E409236T1
Authority
AT
Austria
Prior art keywords
nucleic acids
derivatized nucleic
sample
nucleic acid
derivatized
Prior art date
Application number
AT00950341T
Other languages
English (en)
Inventor
David Englert
Original Assignee
Packard Bioscience Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Packard Bioscience Company filed Critical Packard Bioscience Company
Application granted granted Critical
Publication of ATE409236T1 publication Critical patent/ATE409236T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT00950341T 1999-07-14 2000-07-14 Derivatisierte nukleinsäuren und deren verwendung ATE409236T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14380499P 1999-07-14 1999-07-14

Publications (1)

Publication Number Publication Date
ATE409236T1 true ATE409236T1 (de) 2008-10-15

Family

ID=22505724

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00950341T ATE409236T1 (de) 1999-07-14 2000-07-14 Derivatisierte nukleinsäuren und deren verwendung

Country Status (6)

Country Link
EP (1) EP1194592B1 (de)
AT (1) ATE409236T1 (de)
AU (1) AU6345700A (de)
CA (1) CA2379132C (de)
DE (1) DE60040335D1 (de)
WO (1) WO2001006012A1 (de)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
EP1395805A4 (de) * 2001-06-11 2005-03-09 Illumina Inc Multiplex-detektionsverfahren
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7208295B2 (en) 2001-11-19 2007-04-24 Affymetrix, Inc. Multiplex oligonucleotide addition and target amplification
EP1319718A1 (de) * 2001-12-14 2003-06-18 Keygene N.V. Hochdurchsatzanalyse und Detektion von mehreren Zielsequenzen
US20030235827A1 (en) * 2002-06-25 2003-12-25 Orchid Biosciences, Inc. Methods and compositions for monitoring primer extension and polymorphism detection reactions
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
CA2499360A1 (en) 2002-09-19 2004-04-01 Applera Corporation Methods and compositions for detecting targets
CN1318606C (zh) * 2002-09-19 2007-05-30 阿普里拉股份有限公司 检测靶序列的方法和组合物
WO2004065628A1 (en) * 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
AU2003202671A1 (en) * 2003-01-21 2004-08-13 Guoliang Fu Quantitative multiplex detection of nucleic acids
WO2005026389A2 (en) * 2003-08-29 2005-03-24 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Ligation-based method of analysis of single nucleotide polymorphisms on genomic dna
US8808991B2 (en) 2003-09-02 2014-08-19 Keygene N.V. Ola-based methods for the detection of target nucleic avid sequences
US7531306B2 (en) 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US20060019289A1 (en) * 2004-06-30 2006-01-26 Applera Corporation Compositions and methods for gene expression analysis
US20060029954A1 (en) * 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
WO2012108499A1 (ja) * 2011-02-10 2012-08-16 三菱レイヨン株式会社 核酸の検出方法
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP4219741A3 (de) 2011-09-06 2023-08-23 Gen-Probe Incorporated Geschlossene nukleinsäurestrukturen
DE102011055247A1 (de) * 2011-11-10 2013-05-16 Albert-Ludwigs-Universität Freiburg Multianalyt-Reportersystem
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
CN105473744B (zh) 2013-06-25 2020-06-19 普罗格诺西斯生物科学公司 采用微流控装置的空间编码生物分析
GB2536446B (en) * 2015-03-17 2020-06-03 Salisbury Nhs Found Trust PCR method
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
WO2018186930A1 (en) * 2017-04-06 2018-10-11 MyOmicsDx, Inc Method and kit for constructing nucleic acid library
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894592A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von räumlichen anordnungen mit gradienten
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
ES2946357T3 (es) * 2019-12-23 2023-07-17 10X Genomics Inc Métodos para el análisis espacial usando ligación con molde de ARN
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4549582A3 (de) 2020-04-22 2025-06-18 10x Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
EP4553168A3 (de) 2020-05-22 2025-08-27 10x Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555B1 (de) 2020-12-21 2026-02-25 10X Genomics, Inc. Verfahren zur erfassung von sonden und/oder barcodes
CN114717298A (zh) * 2021-01-06 2022-07-08 上海慧众同康生物科技有限公司 一种多重扩增方法
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
EP4347879B1 (de) 2021-06-03 2025-02-19 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4482979B1 (de) 2022-11-09 2025-10-01 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
WO1996015271A1 (en) * 1994-11-16 1996-05-23 Abbott Laboratories Multiplex ligations-dependent amplification
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
EP1736554B1 (de) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Bestimmung von Unterschieden in der Nukleinsäuresequenz mittels einer Kombination von Ligasebestimmung und Polymerasekettenreaktion
US5935793A (en) * 1996-09-27 1999-08-10 The Chinese University Of Hong Kong Parallel polynucleotide sequencing method using tagged primers
WO1998035058A2 (en) * 1997-02-07 1998-08-13 Ribozyme Pharmaceuticals, Inc. Improved process for detection and quantification of nucleic acid molecules

Also Published As

Publication number Publication date
WO2001006012A1 (en) 2001-01-25
DE60040335D1 (de) 2008-11-06
EP1194592B1 (de) 2008-09-24
CA2379132A1 (en) 2001-01-25
EP1194592A4 (de) 2004-12-08
AU6345700A (en) 2001-02-05
CA2379132C (en) 2011-05-31
EP1194592A1 (de) 2002-04-10

Similar Documents

Publication Publication Date Title
ATE409236T1 (de) Derivatisierte nukleinsäuren und deren verwendung
EP0777750A4 (de) Verfahren mit einer hohen umsatzrate für das auffinden von sequenzen oder genetischen veränderungen in nukleinsäuren
BR9709495A (pt) Sonda para análise de ácidos nucleicos
DE59609524D1 (de) Verfahren zur Genexpressionsanalyse
ATE549415T1 (de) Arrays und verfahren zur deren verwendung
DE69931085D1 (de) System und Verfahren zum Separieren von biologischen Zellen
ATE330032T1 (de) Verfahren zur erhaltung der dns-integrität
DE60125715D1 (de) Verfahren und testsysteme zur identifizierung von elite event gat-zm1 in biologischen proben
ATE263377T1 (de) Vorrichtung für klinische analysen
DK1175511T3 (da) Fremgangsmåde og testsæt til opsamling og opbevaring af biologiske pröver
ATE397752T1 (de) Verfahren und verwendung von anordungen von proteinfixierungsmitteln
BRPI0108364B8 (pt) “receptáculo para receber amostras, e, método para isolar ácido nucleico de sangue total
PT990047E (pt) Metodos e composicoes para a analise de acidos nucleicos por espectroscopia de massa.
ATE420204T1 (de) Verfahren zur detektion von erkrankungen
NO996425L (no) Metode for sekvensanalyse
FI20011877L (fi) Menetelmä ja bioanturi analyysiä varten
DE60000583D1 (de) Verfahren zur identifizierung und/oder quantifizierung einer zielverbindung
FI950487A7 (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamis eksi ja monistamiseksi
AU2002359090A1 (en) High throughput analysis and detection of multiple target sequences
ATE264922T1 (de) Verfahren zur erkennung von klonalen populationen von transformierten zellen in einer genomisch heterogenen zellulären probe
GB2377272A (en) Method and apparatus for DNA sequencing
ATE239274T1 (de) Proteom-analyse zum charakterisieren von auf- und abgeregelten proteinen in biologischen proben
ES2183399T3 (es) Metodo para la determinacion del funcionamiento del higado.
AU4775497A (en) Method for purifying and eventually analyzing nucleic acids from biological test samples
IL143486A0 (en) Array and method for analysing nucleic acid sequences

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties