ES2698903T3 - Oligonucleótido bifuncional para la detección de multianalitos universal en tiempo real - Google Patents
Oligonucleótido bifuncional para la detección de multianalitos universal en tiempo real Download PDFInfo
- Publication number
- ES2698903T3 ES2698903T3 ES12798176T ES12798176T ES2698903T3 ES 2698903 T3 ES2698903 T3 ES 2698903T3 ES 12798176 T ES12798176 T ES 12798176T ES 12798176 T ES12798176 T ES 12798176T ES 2698903 T3 ES2698903 T3 ES 2698903T3
- Authority
- ES
- Spain
- Prior art keywords
- molecule
- region
- probe
- mediator
- identification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102011055247A DE102011055247A1 (de) | 2011-11-10 | 2011-11-10 | Multianalyt-Reportersystem |
| PCT/EP2012/072402 WO2013079307A1 (de) | 2011-11-10 | 2012-11-12 | Bifunktionale oligonukleotidsonde zur universellen echtzeit multianalytdetektion |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2698903T3 true ES2698903T3 (es) | 2019-02-06 |
Family
ID=47324049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12798176T Active ES2698903T3 (es) | 2011-11-10 | 2012-11-12 | Oligonucleótido bifuncional para la detección de multianalitos universal en tiempo real |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US11293053B2 (enExample) |
| EP (1) | EP2776585B8 (enExample) |
| JP (1) | JP6265905B2 (enExample) |
| CN (1) | CN103946397B (enExample) |
| CA (1) | CA2854427A1 (enExample) |
| DE (1) | DE102011055247A1 (enExample) |
| ES (1) | ES2698903T3 (enExample) |
| IN (1) | IN2014DN04645A (enExample) |
| WO (1) | WO2013079307A1 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012100824A1 (de) | 2012-02-01 | 2013-09-05 | Albert-Ludwigs-Universität Freiburg | Gemultiplexte Digital PCR |
| EP2828407A4 (en) * | 2012-03-20 | 2016-02-10 | UNIVERSITé LAVAL | NUCLEINE ACID DETECTION METHOD WITH TARGET-SPECIFIC INDEXING SOLUTIONS WITH SOLVENT SEGMENT FOR DETECTING FLUORESCENCE ON BONDING TO A FISHING SUNDINE |
| US20140272959A1 (en) * | 2013-03-14 | 2014-09-18 | President And Fellows Of Harvard College | Methods of Hybridizing Probes to Genomic DNA |
| EP3521452B1 (en) * | 2013-06-19 | 2021-08-04 | Luminex Corporation | Real-time multiplexed hydrolysis probe assay |
| EP3034619B1 (de) * | 2014-12-16 | 2018-10-17 | microBIOMix GmbH | Modifizierte Oligonukleotide und Verfahren zum Einschleusen von Primern oder Kontrollmolekülen oder Sonden in geschlossene PCR-Systeme |
| WO2016134191A1 (en) * | 2015-02-18 | 2016-08-25 | Singular Bio, Inc. | Assays for single molecule detection and use thereof |
| KR101875662B1 (ko) * | 2015-12-01 | 2018-08-02 | 에스디 바이오센서 주식회사 | 등온 핵산 증폭을 위한 리포터 염료, 퀀처가 포함되는 등온 기반 이중 기능성 올리고뉴클레오타이드 및 이를 이용한 핵산 증폭과 측정 방법 |
| CN107024583B (zh) * | 2016-01-29 | 2019-03-08 | 中国科学院苏州纳米技术与纳米仿生研究所 | 硫黄素t适配体、其筛选方法及应用 |
| BR112019012925A2 (pt) * | 2016-12-23 | 2019-12-10 | Albert-Ludwigs-Universität Freiburg | sonda mediadora em duas partes |
| CN108823287B (zh) * | 2017-04-28 | 2019-04-12 | 厦门大学 | 一种检测靶核酸序列的方法 |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| CN111100935B (zh) * | 2018-10-26 | 2023-03-31 | 厦门大学 | 一种细菌耐药基因检测的方法 |
| KR20230098259A (ko) * | 2020-10-29 | 2023-07-03 | 비오까르띠 엔브이 | 멀티플렉스 핵산 검출을 위한 제네릭 카트리지 및 방법 |
| WO2023108014A1 (en) * | 2021-12-07 | 2023-06-15 | Luminex Corporation | Methods and compositions for nucleic acid analysis |
| CN116356075A (zh) * | 2021-12-27 | 2023-06-30 | 迈克生物股份有限公司 | 一种用于检测的引物探针、引物探针组及其应用 |
| EP4265734A1 (de) * | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Nukleinsäurenachweis in einer pcr mittels eines zielsequenz-unspezifischem modularer reporterkomplexes |
| EP4265735A1 (de) | 2022-04-22 | 2023-10-25 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheid-baren signalstäken |
| EP4511511A1 (de) | 2022-04-22 | 2025-02-26 | Hahn-Schickard-Gesellschaft für angewandte Forschung e. V. | Pcr-multiplexing durch zielsequenz-unabhängige-reportermoleküle mit unterscheidbaren signalstärken |
| US20250340928A1 (en) | 2022-04-22 | 2025-11-06 | Albert-Ludwigs-Universität Freiburg | Nucleic acid detection in a pcr by means of a target-sequence-unspecific modular reporter complex |
| EP4372100A1 (en) | 2022-11-15 | 2024-05-22 | Stilla Technologies | Data analysis method for multiplex digital pcr using color-combination |
| EP4754292A1 (en) * | 2023-07-31 | 2026-06-10 | Coyote Bioscience Co., Ltd. | Compositions and methods for nucleic acid detection |
| CN117144067A (zh) * | 2023-10-31 | 2023-12-01 | 中国医学科学院北京协和医院 | 用于多重化核酸检测的组合物和方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US5846708A (en) | 1991-11-19 | 1998-12-08 | Massachusetts Institiute Of Technology | Optical and electrical methods and apparatus for molecule detection |
| US6099803A (en) | 1994-07-07 | 2000-08-08 | Nanogen, Inc. | Advanced active electronic devices for molecular biological analysis and diagnostics |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| GB9615516D0 (en) * | 1996-07-24 | 1996-09-04 | Jsd Tech Ltd | Bacillus cereus |
| US6238869B1 (en) | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
| AU2849800A (en) | 1999-01-15 | 2000-08-01 | Ljl Biosystems, Inc. | Methods and apparatus for detecting polynucleotide hybridization |
| US6300070B1 (en) | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
| ATE409236T1 (de) * | 1999-07-14 | 2008-10-15 | Packard Bioscience Company | Derivatisierte nukleinsäuren und deren verwendung |
| US6380377B1 (en) * | 2000-07-14 | 2002-04-30 | Applied Gene Technologies, Inc. | Nucleic acid hairpin probes and uses thereof |
| DE60131903T2 (de) * | 2000-10-24 | 2008-11-27 | The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto | Direkte multiplex charakterisierung von genomischer dna |
| EP1478783A4 (en) * | 2002-02-06 | 2006-12-27 | Ge Healthcare Bio Sciences Ab | COMPOSITIONS AND METHODS OF ROLLING CIRCLE AMPLIFICATION |
| GB0230238D0 (en) * | 2002-12-28 | 2003-02-05 | Fu Guoliang | Combined exponential and linear amplification |
| DE10316159A1 (de) | 2003-04-09 | 2004-10-28 | Klapproth, Holger, Dr. | 5`-3`-Exonuklease Assay zur Bestimmung der Konzentration von Nukleinsäuren |
| JP5520449B2 (ja) | 2007-03-06 | 2014-06-11 | 株式会社ビー・エム・エル | 核酸の検出方法 |
| BRPI0908748A2 (pt) * | 2008-03-15 | 2015-07-28 | Hologic Inc | Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação |
| MX352460B (es) | 2011-01-11 | 2017-11-24 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
-
2011
- 2011-11-10 DE DE102011055247A patent/DE102011055247A1/de not_active Withdrawn
-
2012
- 2012-11-12 JP JP2014540506A patent/JP6265905B2/ja active Active
- 2012-11-12 US US14/357,574 patent/US11293053B2/en active Active
- 2012-11-12 IN IN4645DEN2014 patent/IN2014DN04645A/en unknown
- 2012-11-12 EP EP12798176.9A patent/EP2776585B8/de active Active
- 2012-11-12 CA CA2854427A patent/CA2854427A1/en not_active Abandoned
- 2012-11-12 WO PCT/EP2012/072402 patent/WO2013079307A1/de not_active Ceased
- 2012-11-12 ES ES12798176T patent/ES2698903T3/es active Active
- 2012-11-12 CN CN201280055550.2A patent/CN103946397B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103946397A (zh) | 2014-07-23 |
| JP6265905B2 (ja) | 2018-01-24 |
| WO2013079307A1 (de) | 2013-06-06 |
| IN2014DN04645A (enExample) | 2015-05-08 |
| EP2776585B8 (de) | 2019-04-10 |
| JP2014533107A (ja) | 2014-12-11 |
| US20140315747A1 (en) | 2014-10-23 |
| US11293053B2 (en) | 2022-04-05 |
| DE102011055247A1 (de) | 2013-05-16 |
| CA2854427A1 (en) | 2013-06-06 |
| EP2776585B1 (de) | 2018-08-22 |
| EP2776585A1 (de) | 2014-09-17 |
| CN103946397B (zh) | 2021-03-16 |
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