JP6176922B2 - 基質特異性が改変されたアマドリアーゼ - Google Patents

基質特異性が改変されたアマドリアーゼ Download PDF

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JP6176922B2
JP6176922B2 JP2012527775A JP2012527775A JP6176922B2 JP 6176922 B2 JP6176922 B2 JP 6176922B2 JP 2012527775 A JP2012527775 A JP 2012527775A JP 2012527775 A JP2012527775 A JP 2012527775A JP 6176922 B2 JP6176922 B2 JP 6176922B2
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amino acid
acid sequence
amadoriase
seq
substitution
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JPWO2012018094A1 (ja
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敦 一柳
敦 一柳
浩三 廣川
浩三 廣川
康子 田鍋
康子 田鍋
陽介 鉞
陽介 鉞
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Kikkoman Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012527775A 2010-08-06 2011-08-04 基質特異性が改変されたアマドリアーゼ Active JP6176922B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2010176967 2010-08-06
JP2010176967 2010-08-06
JP2010213070 2010-09-24
JP2010213070 2010-09-24
PCT/JP2011/067898 WO2012018094A1 (ja) 2010-08-06 2011-08-04 基質特異性が改変されたアマドリアーゼ

Related Child Applications (1)

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JP2017081507A Division JP6538101B2 (ja) 2010-08-06 2017-04-17 基質特異性が改変されたアマドリアーゼ

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JPWO2012018094A1 JPWO2012018094A1 (ja) 2013-10-03
JP6176922B2 true JP6176922B2 (ja) 2017-08-09

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US (1) US9062286B2 (enExample)
EP (1) EP2602318B1 (enExample)
JP (2) JP6176922B2 (enExample)
KR (1) KR102070990B1 (enExample)
CN (1) CN103080308B (enExample)
WO (1) WO2012018094A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017121261A (ja) * 2010-08-06 2017-07-13 キッコーマン株式会社 基質特異性が改変されたアマドリアーゼ

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013100006A1 (ja) 2011-12-28 2013-07-04 キッコーマン株式会社 熱安定性が向上したアマドリアーゼ、その遺伝子および組換えｄｎａならびに熱安定性が向上したアマドリアーゼの製造法
JP6068916B2 (ja) * 2012-02-02 2017-01-25 キッコーマン株式会社 糖化ヘモグロビン測定用試薬組成物および糖化ヘモグロビン測定方法
EP3508577A3 (en) 2012-04-27 2019-09-04 Kikkoman Corporation Modified amadoriase capable of acting on fructosyl hexapeptide
CN103937763B (zh) * 2013-01-22 2016-01-06 清华大学 R112w型酮胺氧化酶及其编码基因和应用
JP6363327B2 (ja) * 2013-03-25 2018-07-25 キッコーマン株式会社 改変アマドリアーゼ
EP3031914B1 (en) 2013-08-09 2020-12-23 Kikkoman Corporation Modified amadoriase and method for producing the same, agent for improving surfactant resistance of amadoriase and composition for measuring hba1c using the same
WO2015060431A1 (ja) 2013-10-25 2015-04-30 キッコーマン株式会社 糖化ペプチドに作用するアマドリアーゼを用いたＨｂＡ１ｃ測定法
EP3061819B1 (en) * 2013-10-25 2023-05-24 Kikkoman Corporation HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT
JP6749745B2 (ja) * 2014-02-27 2020-09-02 キッコーマン株式会社 アマドリアーゼ含有組成物の安定化方法、熱安定性が向上したアマドリアーゼ含有組成物及びこれを用いた糖化ヘモグロビン測定用組成物
KR102159807B1 (ko) * 2014-10-24 2020-09-25 기꼬만 가부시키가이샤 디히드로게나아제 활성이 향상된 아마도리아제
WO2016072520A1 (ja) * 2014-11-07 2016-05-12 キッコーマン株式会社 アニオン性界面活性剤耐性が向上したアマドリアーゼ
WO2016199898A1 (ja) * 2015-06-10 2016-12-15 公立大学法人 富山県立大学 活性型変異酵素の製造方法および新規活性型変異酵素、並びに可溶性化変異タンパク質の製造方法
KR102596402B1 (ko) * 2016-04-22 2023-10-31 기꼬만 가부시키가이샤 HbA1c 디히드로게나아제
EP3336179B1 (en) 2016-12-13 2020-02-05 Industrial Technology Research Institute Recombinant protein and preparation method and application thereof
US11142778B2 (en) * 2017-07-24 2021-10-12 Riken Decarboxylase and method for producing unsaturated hydrocarbon compound using same
WO2019045052A1 (ja) 2017-08-31 2019-03-07 キッコーマン株式会社 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
JP6818718B2 (ja) * 2018-06-28 2021-01-20 キッコーマン株式会社 改変アマドリアーゼ
CN118369420A (zh) 2022-01-27 2024-07-19 株式会社普欧威盖特 果糖基氨基酸氧化酶、果糖基氨基酸氧化酶的制造方法、糖化蛋白传感器和糖化蛋白的测定方法

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017121261A (ja) * 2010-08-06 2017-07-13 キッコーマン株式会社 基質特異性が改変されたアマドリアーゼ

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Publication number Publication date
JP6538101B2 (ja) 2019-07-03
EP2602318B1 (en) 2015-09-30
KR102070990B1 (ko) 2020-01-29
KR20130105614A (ko) 2013-09-25
JPWO2012018094A1 (ja) 2013-10-03
CN103080308B (zh) 2015-08-05
EP2602318A1 (en) 2013-06-12
JP2017121261A (ja) 2017-07-13
CN103080308A (zh) 2013-05-01
WO2012018094A1 (ja) 2012-02-09
US9062286B2 (en) 2015-06-23
EP2602318A4 (en) 2014-02-26
US20130267007A1 (en) 2013-10-10

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