JP6099627B2 - 発現方法 - Google Patents

発現方法 Download PDF

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Publication number
JP6099627B2
JP6099627B2 JP2014504258A JP2014504258A JP6099627B2 JP 6099627 B2 JP6099627 B2 JP 6099627B2 JP 2014504258 A JP2014504258 A JP 2014504258A JP 2014504258 A JP2014504258 A JP 2014504258A JP 6099627 B2 JP6099627 B2 JP 6099627B2
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Japan
Prior art keywords
bacillus
protease
microorganism
protein
acid sequence
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Expired - Fee Related
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JP2014504258A
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English (en)
Japanese (ja)
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JP2014511702A (ja
Inventor
マクシム ルーカス
マクシム ルーカス
クナープ ラモーナ
クナープ ラモーナ
エーファース シュテファン
エーファース シュテファン
マウラー カール−ハインツ
マウラー カール−ハインツ
ボンゲアツ ヨハネス
ボンゲアツ ヨハネス
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BASF SE
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BASF SE
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2014504258A 2011-04-13 2012-04-05 発現方法 Expired - Fee Related JP6099627B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102011007313A DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren
DE102011007313.2 2011-04-13
PCT/EP2012/056262 WO2012139964A1 (de) 2011-04-13 2012-04-05 Expressionsverfahren

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016243563A Division JP2017079768A (ja) 2011-04-13 2016-12-15 発現方法

Publications (2)

Publication Number Publication Date
JP2014511702A JP2014511702A (ja) 2014-05-19
JP6099627B2 true JP6099627B2 (ja) 2017-03-22

Family

ID=45952535

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2014504258A Expired - Fee Related JP6099627B2 (ja) 2011-04-13 2012-04-05 発現方法
JP2016243563A Withdrawn JP2017079768A (ja) 2011-04-13 2016-12-15 発現方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016243563A Withdrawn JP2017079768A (ja) 2011-04-13 2016-12-15 発現方法

Country Status (14)

Country Link
US (1) US9663798B2 (en22)
EP (1) EP2697376B1 (en22)
JP (2) JP6099627B2 (en22)
KR (1) KR20140019436A (en22)
CN (1) CN103608457A (en22)
BR (1) BR112013025951A2 (en22)
CA (1) CA2831473A1 (en22)
DE (1) DE102011007313A1 (en22)
DK (1) DK2697376T3 (en22)
ES (1) ES2639114T3 (en22)
MX (1) MX348766B (en22)
PL (1) PL2697376T3 (en22)
RU (1) RU2642324C2 (en22)
WO (1) WO2012139964A1 (en22)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
CN104059869B (zh) * 2014-06-20 2016-07-06 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
KR102112240B1 (ko) * 2018-09-28 2020-05-18 씨제이제일제당 주식회사 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
DE69033388T2 (de) 1989-08-25 2000-05-11 Henkel Research Corp Alkalisches proteolytisches enzym und verfahren zur herstellung
DE69131980T3 (de) 1991-06-11 2013-02-07 Genencor International, Inc. Cellulasezusammensetzungen mit einem defizit an komponenten des typs-cbh i enthaltende reinigungsmittelzusammensetzungen
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
ES2302330T3 (es) * 1994-02-24 2008-07-01 Henkel Kommanditgesellschaft Auf Aktien Enzimas mejoradas y detergentes que las contienen.
EP1683860B1 (en) 1995-03-17 2013-10-23 Novozymes A/S Novel endoglucanases
ES2525677T3 (es) 1995-10-17 2014-12-29 Ab Enzymes Oy Celulasas, genes que las codifican y usos de las mismas
WO1998012307A1 (en) 1996-09-17 1998-03-26 Novo Nordisk A/S Cellulase variants
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
ES2532606T3 (es) 1999-03-31 2015-03-30 Novozymes A/S Polipéptidos con actividad de alfa-amilasa alcalina y ácidos nucleicos que los codifican
AU2452101A (en) 1999-12-23 2001-07-03 Pharmacia & Upjohn Company Assays and methods of diagnosis and treatment based on use of sodium channels astargets for amyloid beta or its aggregates
CA2417857C (en) 2000-08-04 2012-03-06 Genencor International, Inc. Mutant egiii cellulase, dna encoding such egiii compositions and methods for obtaining same
WO2002099091A2 (en) 2001-06-06 2002-12-12 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
AU2003210552A1 (en) * 2002-01-16 2003-09-02 Genencor International, Inc. Multiply-substituted protease variants
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
DE10309555A1 (de) * 2003-03-04 2004-09-23 Henkel Kgaa Faktor PrfB (Peptide chain release factor B) aus Bacillus licheniformis und Steigerung der Proteinbildungsrate durch Erhöhung der PrfB-Aktivität
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
DE102004013843A1 (de) * 2004-03-20 2005-10-13 Degussa Ag Expression von Nitrilhydratasen im Zwei-Vektor-Expressionssystem
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
US20080199443A1 (en) * 2005-06-09 2008-08-21 The Govt. Of The U.S.A. As Represented By The Secretary, Dept. Of Health And Human Services Bone Morphogenetic Variants, Compositions and Methods of Treatment
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
EP2013339B1 (en) * 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
EP1873251A1 (en) * 2006-06-29 2008-01-02 Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus Expression vector(s) for enhanced expression of a protein of interest in eukaryotic or prokaryotic host cells
CN101511998A (zh) * 2006-07-18 2009-08-19 丹尼斯科美国公司 在宽温度范围内具有活性的蛋白酶变体
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus

Also Published As

Publication number Publication date
CN103608457A (zh) 2014-02-26
JP2014511702A (ja) 2014-05-19
RU2642324C2 (ru) 2018-01-24
MX348766B (es) 2017-06-28
US20140220628A1 (en) 2014-08-07
MX2013011816A (es) 2013-10-25
PL2697376T3 (pl) 2017-10-31
DK2697376T3 (en) 2017-09-18
DE102011007313A1 (de) 2012-10-18
CA2831473A1 (en) 2012-10-18
EP2697376A1 (de) 2014-02-19
JP2017079768A (ja) 2017-05-18
ES2639114T3 (es) 2017-10-25
KR20140019436A (ko) 2014-02-14
US9663798B2 (en) 2017-05-30
WO2012139964A1 (de) 2012-10-18
RU2013150276A (ru) 2015-05-20
BR112013025951A2 (pt) 2017-08-01
EP2697376B1 (de) 2017-05-31

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