DE102011007313A1 - Expressionsverfahren - Google Patents

Expressionsverfahren Download PDF

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Publication number
DE102011007313A1
DE102011007313A1 DE102011007313A DE102011007313A DE102011007313A1 DE 102011007313 A1 DE102011007313 A1 DE 102011007313A1 DE 102011007313 A DE102011007313 A DE 102011007313A DE 102011007313 A DE102011007313 A DE 102011007313A DE 102011007313 A1 DE102011007313 A1 DE 102011007313A1
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Germany
Prior art keywords
microorganism
protein
protease
bacillus
auxiliary
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Withdrawn
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DE102011007313A
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German (de)
English (en)
Inventor
Lukas Maksym
Knab Ramona
Stefan Evers
Dr. Maurer Karl-Heinz
Dr. Bongaerts Johannes
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BASF SE
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Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Priority to DE102011007313A priority Critical patent/DE102011007313A1/de
Priority to DK12713714.9T priority patent/DK2697376T3/en
Priority to ES12713714.9T priority patent/ES2639114T3/es
Priority to BR112013025951A priority patent/BR112013025951A2/pt
Priority to CA2831473A priority patent/CA2831473A1/en
Priority to MX2013011816A priority patent/MX348766B/es
Priority to RU2013150276A priority patent/RU2642324C2/ru
Priority to CN201280028836.1A priority patent/CN103608457A/zh
Priority to PCT/EP2012/056262 priority patent/WO2012139964A1/de
Priority to EP12713714.9A priority patent/EP2697376B1/de
Priority to KR1020137029683A priority patent/KR20140019436A/ko
Priority to PL12713714T priority patent/PL2697376T3/pl
Priority to US14/110,724 priority patent/US9663798B2/en
Priority to JP2014504258A priority patent/JP6099627B2/ja
Publication of DE102011007313A1 publication Critical patent/DE102011007313A1/de
Priority to JP2016243563A priority patent/JP2017079768A/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE102011007313A 2011-04-13 2011-04-13 Expressionsverfahren Withdrawn DE102011007313A1 (de)

Priority Applications (15)

Application Number Priority Date Filing Date Title
DE102011007313A DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren
CN201280028836.1A CN103608457A (zh) 2011-04-13 2012-04-05 表达方法
PCT/EP2012/056262 WO2012139964A1 (de) 2011-04-13 2012-04-05 Expressionsverfahren
BR112013025951A BR112013025951A2 (pt) 2011-04-13 2012-04-05 método para fabricação de uma proteína através de um micro-organismo, micro-organismo e uso
CA2831473A CA2831473A1 (en) 2011-04-13 2012-04-05 Expression method
MX2013011816A MX348766B (es) 2011-04-13 2012-04-05 Metodo de expresion.
RU2013150276A RU2642324C2 (ru) 2011-04-13 2012-04-05 Способ экспрессии
DK12713714.9T DK2697376T3 (en) 2011-04-13 2012-04-05 EXPRESSION PROCEDURE WITH A HELP PROTASE
ES12713714.9T ES2639114T3 (es) 2011-04-13 2012-04-05 Procedimiento de expresión con una proteasa auxiliar
EP12713714.9A EP2697376B1 (de) 2011-04-13 2012-04-05 Expressionsverfahren mit einer hilfsprotease
KR1020137029683A KR20140019436A (ko) 2011-04-13 2012-04-05 발현 방법
PL12713714T PL2697376T3 (pl) 2011-04-13 2012-04-05 Sposób ekspresji z pomocą proteazy pomocniczej
US14/110,724 US9663798B2 (en) 2011-04-13 2012-04-05 Expression method
JP2014504258A JP6099627B2 (ja) 2011-04-13 2012-04-05 発現方法
JP2016243563A JP2017079768A (ja) 2011-04-13 2016-12-15 発現方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102011007313A DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren

Publications (1)

Publication Number Publication Date
DE102011007313A1 true DE102011007313A1 (de) 2012-10-18

Family

ID=45952535

Family Applications (1)

Application Number Title Priority Date Filing Date
DE102011007313A Withdrawn DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren

Country Status (14)

Country Link
US (1) US9663798B2 (en22)
EP (1) EP2697376B1 (en22)
JP (2) JP6099627B2 (en22)
KR (1) KR20140019436A (en22)
CN (1) CN103608457A (en22)
BR (1) BR112013025951A2 (en22)
CA (1) CA2831473A1 (en22)
DE (1) DE102011007313A1 (en22)
DK (1) DK2697376T3 (en22)
ES (1) ES2639114T3 (en22)
MX (1) MX348766B (en22)
PL (1) PL2697376T3 (en22)
RU (1) RU2642324C2 (en22)
WO (1) WO2012139964A1 (en22)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
CN104059869B (zh) * 2014-06-20 2016-07-06 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
KR102112240B1 (ko) * 2018-09-28 2020-05-18 씨제이제일제당 주식회사 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability

Citations (19)

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Publication number Priority date Publication date Assignee Title
WO1991002792A1 (en) 1989-08-25 1991-03-07 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
WO1992006165A1 (en) 1991-06-11 1992-04-16 Genencor International, Inc. Detergent compositions containing cellulase compositions deficient in cbh i type components
WO1996029397A1 (en) 1995-03-17 1996-09-26 Novo Nordisk A/S Novel endoglucanases
WO1997014804A1 (en) 1995-10-17 1997-04-24 Röhn Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
WO1998012307A1 (en) 1996-09-17 1998-03-26 Novo Nordisk A/S Cellulase variants
WO1998045398A1 (de) 1997-04-04 1998-10-15 Henkel Kommanditgesellschaft Auf Aktien Aktivatoren für persauerstoffverbindungen in wasch- und reinigungsmitteln
WO2000060060A2 (en) 1999-03-31 2000-10-12 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
WO2002099091A2 (en) 2001-06-06 2002-12-12 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
WO2003002711A2 (de) 2001-06-29 2003-01-09 Henkel Kommanditgesellschaft Auf Aktien EINE NEUE GRUPPE VON α-AMYLASEN SOWIE EIN VERFAHREN ZUR IDENTIFIZIERUNG UND GEWINNUNG NEUER α-AMYLASEN
EP1305432A2 (en) 2000-08-04 2003-05-02 Genencor International, Inc. Mutant trichoderma reesei egiii cellulases, dna encoding such egiii compositions and methods for obtaining same
WO2003054177A2 (de) 2001-12-21 2003-07-03 Henkel Kommanditgesellschaft Auf Aktien Neue glykosylhydrolasen
WO2004058961A1 (de) 2002-12-20 2004-07-15 Henkel Kommanditgesellschaft Auf Aktien Subtilisin-varianten, bei denen die perhydrolase-aktivität erhöht wurde
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
WO2005124012A1 (de) 2004-06-18 2005-12-29 Henkel Kommanditgesellschaft Auf Aktien Neues enzymatisches bleichsystem
WO2007079938A2 (de) 2005-12-28 2007-07-19 Henkel Ag & Co. Kgaa Wasch- oder reinigungsmittel mit spezieller amylase
WO2007131656A1 (de) 2006-05-11 2007-11-22 Henkel Ag & Co. Kgaa Subtilisin aus bacillus pumilus und wasch- und reinigungsmittel enthaltend dieses neue subtilisin
WO2008086916A1 (de) 2007-01-16 2008-07-24 Henkel Ag & Co. Kgaa Neue alkalische protease aus bacillus gibsonii und wasch- und reinigungsmittel enthaltend diese neue alkalische protease
EP1727902B1 (en) * 2004-03-20 2008-09-03 BRAIN Biotechnology Research and Information Network Aktiengesellschaft Expression of nitrile hydratases in a two-vector expression system

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
ES2302330T3 (es) * 1994-02-24 2008-07-01 Henkel Kommanditgesellschaft Auf Aktien Enzimas mejoradas y detergentes que las contienen.
AU2003210552A1 (en) * 2002-01-16 2003-09-02 Genencor International, Inc. Multiply-substituted protease variants
DE10309555A1 (de) * 2003-03-04 2004-09-23 Henkel Kgaa Faktor PrfB (Peptide chain release factor B) aus Bacillus licheniformis und Steigerung der Proteinbildungsrate durch Erhöhung der PrfB-Aktivität
US20080199443A1 (en) * 2005-06-09 2008-08-21 The Govt. Of The U.S.A. As Represented By The Secretary, Dept. Of Health And Human Services Bone Morphogenetic Variants, Compositions and Methods of Treatment
EP2013339B1 (en) * 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
EP1873251A1 (en) * 2006-06-29 2008-01-02 Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus Expression vector(s) for enhanced expression of a protein of interest in eukaryotic or prokaryotic host cells
CN101511998A (zh) * 2006-07-18 2009-08-19 丹尼斯科美国公司 在宽温度范围内具有活性的蛋白酶变体
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002792A1 (en) 1989-08-25 1991-03-07 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
WO1992006165A1 (en) 1991-06-11 1992-04-16 Genencor International, Inc. Detergent compositions containing cellulase compositions deficient in cbh i type components
WO1996029397A1 (en) 1995-03-17 1996-09-26 Novo Nordisk A/S Novel endoglucanases
WO1997014804A1 (en) 1995-10-17 1997-04-24 Röhn Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
WO1998012307A1 (en) 1996-09-17 1998-03-26 Novo Nordisk A/S Cellulase variants
WO1998045398A1 (de) 1997-04-04 1998-10-15 Henkel Kommanditgesellschaft Auf Aktien Aktivatoren für persauerstoffverbindungen in wasch- und reinigungsmitteln
WO2000060060A2 (en) 1999-03-31 2000-10-12 Novozymes A/S Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
EP1305432A2 (en) 2000-08-04 2003-05-02 Genencor International, Inc. Mutant trichoderma reesei egiii cellulases, dna encoding such egiii compositions and methods for obtaining same
WO2002099091A2 (en) 2001-06-06 2002-12-12 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
WO2003002711A2 (de) 2001-06-29 2003-01-09 Henkel Kommanditgesellschaft Auf Aktien EINE NEUE GRUPPE VON α-AMYLASEN SOWIE EIN VERFAHREN ZUR IDENTIFIZIERUNG UND GEWINNUNG NEUER α-AMYLASEN
WO2003054177A2 (de) 2001-12-21 2003-07-03 Henkel Kommanditgesellschaft Auf Aktien Neue glykosylhydrolasen
WO2004058961A1 (de) 2002-12-20 2004-07-15 Henkel Kommanditgesellschaft Auf Aktien Subtilisin-varianten, bei denen die perhydrolase-aktivität erhöht wurde
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
EP1727902B1 (en) * 2004-03-20 2008-09-03 BRAIN Biotechnology Research and Information Network Aktiengesellschaft Expression of nitrile hydratases in a two-vector expression system
WO2005124012A1 (de) 2004-06-18 2005-12-29 Henkel Kommanditgesellschaft Auf Aktien Neues enzymatisches bleichsystem
WO2007079938A2 (de) 2005-12-28 2007-07-19 Henkel Ag & Co. Kgaa Wasch- oder reinigungsmittel mit spezieller amylase
WO2007131656A1 (de) 2006-05-11 2007-11-22 Henkel Ag & Co. Kgaa Subtilisin aus bacillus pumilus und wasch- und reinigungsmittel enthaltend dieses neue subtilisin
WO2008086916A1 (de) 2007-01-16 2008-07-24 Henkel Ag & Co. Kgaa Neue alkalische protease aus bacillus gibsonii und wasch- und reinigungsmittel enthaltend diese neue alkalische protease

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Altschul, Stephan F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Hheng Zhang, Webb Miller, and David J. Lipman (1997): "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs"; Nucleic Acids Res., 25, S. 3389-3402
Bernhard et al., J. Bacteriol. (133), S. 897 ff, (1978)
Chenna et al. (2003): Multiple sequence alignment with the Clustal series of programs. Nucleic Acid Research 31, 3497-3500
Coexpression of Proteins in Bacteria Using T7-Based Expression Plasmids: Expression of Heterodimeric Cell-Cycle and Transcriptional Regulatrry Complexes *
Del Mar et al., 1979
Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases *
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Tenside, Band 7 (1970), S. 125-132

Also Published As

Publication number Publication date
CN103608457A (zh) 2014-02-26
JP2014511702A (ja) 2014-05-19
RU2642324C2 (ru) 2018-01-24
JP6099627B2 (ja) 2017-03-22
MX348766B (es) 2017-06-28
US20140220628A1 (en) 2014-08-07
MX2013011816A (es) 2013-10-25
PL2697376T3 (pl) 2017-10-31
DK2697376T3 (en) 2017-09-18
CA2831473A1 (en) 2012-10-18
EP2697376A1 (de) 2014-02-19
JP2017079768A (ja) 2017-05-18
ES2639114T3 (es) 2017-10-25
KR20140019436A (ko) 2014-02-14
US9663798B2 (en) 2017-05-30
WO2012139964A1 (de) 2012-10-18
RU2013150276A (ru) 2015-05-20
BR112013025951A2 (pt) 2017-08-01
EP2697376B1 (de) 2017-05-31

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