MX348766B - Metodo de expresion. - Google Patents

Metodo de expresion.

Info

Publication number
MX348766B
MX348766B MX2013011816A MX2013011816A MX348766B MX 348766 B MX348766 B MX 348766B MX 2013011816 A MX2013011816 A MX 2013011816A MX 2013011816 A MX2013011816 A MX 2013011816A MX 348766 B MX348766 B MX 348766B
Authority
MX
Mexico
Prior art keywords
protein
expression construct
aim
amino acid
acid sequence
Prior art date
Application number
MX2013011816A
Other languages
English (en)
Other versions
MX2013011816A (es
Inventor
Maksym Lukas
Knab Ramona
Evers Stefan
Maurer Karl-Heinz
Bongaerts Johannes
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of MX2013011816A publication Critical patent/MX2013011816A/es
Publication of MX348766B publication Critical patent/MX348766B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una método para preparar una proteína por medio de un microorganismo, caracterizado porque comprende las etapas de: (a) introducir en un microorganismo una primera construcción de expresión que codifica la proteína; (b) introducir en el microorganismo una segunda construcción de expresión que codifica una proteasa auxiliar que difiere de la proteína y que comprende una secuencia de aminoácidos que es por lo menos 90% idéntica a la secuencia de aminoácidos indicada en la SEC ID NO. 1, en donde la proteasa auxiliar tiene actividad proteolítica y en donde la segunda construcción de expresión se introduce en una secuencia de ácido nucleico cromosómico que codifica una proteasa del microorganismo de manera que la proteasa codificada por el cromosoma está funcionalmente inactiva; y (c) expresar la proteína y la proteasa auxiliar en el microorganismo, en donde la proteína es una enzima, particularmente una proteasa, amilasa, celulasa, hemicelulasa, mananasa, tanasa, xilanasa, xantanasa, xiloglucanasa, betaglucosida, pectinasa, carragenasa, perhidrolasa, oxidasa, oxidoreductasa o una lipasa.
MX2013011816A 2011-04-13 2012-04-05 Metodo de expresion. MX348766B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011007313A DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren
PCT/EP2012/056262 WO2012139964A1 (de) 2011-04-13 2012-04-05 Expressionsverfahren

Publications (2)

Publication Number Publication Date
MX2013011816A MX2013011816A (es) 2013-10-25
MX348766B true MX348766B (es) 2017-06-28

Family

ID=45952535

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013011816A MX348766B (es) 2011-04-13 2012-04-05 Metodo de expresion.

Country Status (14)

Country Link
US (1) US9663798B2 (es)
EP (1) EP2697376B1 (es)
JP (2) JP6099627B2 (es)
KR (1) KR20140019436A (es)
CN (1) CN103608457A (es)
BR (1) BR112013025951A2 (es)
CA (1) CA2831473A1 (es)
DE (1) DE102011007313A1 (es)
DK (1) DK2697376T3 (es)
ES (1) ES2639114T3 (es)
MX (1) MX348766B (es)
PL (1) PL2697376T3 (es)
RU (1) RU2642324C2 (es)
WO (1) WO2012139964A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
CN104059869B (zh) * 2014-06-20 2016-07-06 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用
CN111373039A (zh) 2017-11-29 2020-07-03 丹尼斯科美国公司 具有改善的稳定性的枯草杆菌蛋白酶变体
KR102112240B1 (ko) * 2018-09-28 2020-05-18 씨제이제일제당 주식회사 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법
WO2020112599A1 (en) 2018-11-28 2020-06-04 Danisco Us Inc Subtilisin variants having improved stability

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DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
WO1991002792A1 (en) 1989-08-25 1991-03-07 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
ATE469203T1 (de) 1991-06-11 2010-06-15 Genencor Int Cellulasezusammensetzungen mit einem defizit an komponenten des typs-cbh i enthaltende reinigungsmittelzusammensetzungen
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
DE69535736T2 (de) * 1994-02-24 2009-04-30 Henkel Ag & Co. Kgaa Verbesserte enzyme und diese enthaltene detergentien
NZ303162A (en) 1995-03-17 2000-01-28 Novo Nordisk As Enzyme preparations comprising an enzyme exhibiting endoglucanase activity appropriate for laundry compositions for textiles
WO1997014804A1 (en) 1995-10-17 1997-04-24 Röhn Enzyme Finland OY Cellulases, the genes encoding them and uses thereof
EP1726644A1 (en) 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
DK2011864T3 (en) 1999-03-31 2015-04-07 Novozymes As Polypeptides with alkaline alpha-amylase activity and nucleic acids encoding them
AU2452101A (en) 1999-12-23 2001-07-03 Pharmacia & Upjohn Company Assays and methods of diagnosis and treatment based on use of sodium channels astargets for amyloid beta or its aggregates
AU2001280920A1 (en) 2000-08-04 2002-02-18 Genencor International, Inc. Mutant trichoderma reesei egiii cellulases, dna encoding such egiii compositions and methods for obtaining same
MXPA03011194A (es) 2001-06-06 2004-02-26 Novozymes As Endo-beta-1,4-glucanasa.
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
EP1523553B1 (en) * 2002-01-16 2009-12-02 Genencor International, Inc. Multiply-substituted protease variants
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
DE10309555A1 (de) * 2003-03-04 2004-09-23 Henkel Kgaa Faktor PrfB (Peptide chain release factor B) aus Bacillus licheniformis und Steigerung der Proteinbildungsrate durch Erhöhung der PrfB-Aktivität
ES2361838T3 (es) 2003-12-03 2011-06-22 Danisco Us Inc. Perhidrolasa.
DE102004013843A1 (de) * 2004-03-20 2005-10-13 Degussa Ag Expression von Nitrilhydratasen im Zwei-Vektor-Expressionssystem
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
WO2006133457A2 (en) * 2005-06-09 2006-12-14 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Bone morphogenetic variants, compositions and methods of treatment
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
EP2013339B1 (en) * 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
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CN104232365A (zh) * 2006-07-18 2014-12-24 丹尼斯科美国公司 在宽温度范围内具有活性的蛋白酶变体
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
KR20100098652A (ko) * 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산

Also Published As

Publication number Publication date
CA2831473A1 (en) 2012-10-18
WO2012139964A1 (de) 2012-10-18
RU2642324C2 (ru) 2018-01-24
DK2697376T3 (en) 2017-09-18
PL2697376T3 (pl) 2017-10-31
CN103608457A (zh) 2014-02-26
MX2013011816A (es) 2013-10-25
US20140220628A1 (en) 2014-08-07
EP2697376A1 (de) 2014-02-19
JP6099627B2 (ja) 2017-03-22
JP2014511702A (ja) 2014-05-19
DE102011007313A1 (de) 2012-10-18
ES2639114T3 (es) 2017-10-25
US9663798B2 (en) 2017-05-30
RU2013150276A (ru) 2015-05-20
KR20140019436A (ko) 2014-02-14
JP2017079768A (ja) 2017-05-18
BR112013025951A2 (pt) 2017-08-01
EP2697376B1 (de) 2017-05-31

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