MX351354B - Método de expresión. - Google Patents

Método de expresión.

Info

Publication number
MX351354B
MX351354B MX2014009118A MX2014009118A MX351354B MX 351354 B MX351354 B MX 351354B MX 2014009118 A MX2014009118 A MX 2014009118A MX 2014009118 A MX2014009118 A MX 2014009118A MX 351354 B MX351354 B MX 351354B
Authority
MX
Mexico
Prior art keywords
protein
expression construct
aim
expression method
microorganism
Prior art date
Application number
MX2014009118A
Other languages
English (en)
Other versions
MX2014009118A (es
Inventor
Evers Stefan
Maurer Karl-Heinz
Bongaerts Johannes
Hellmuth Hendrik
Küppers Tobias
Steffen Victoria
Charlott Eichstädt Renée
Weber Thomas
O'connell Timothy
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47683703&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX351354(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Basf Se filed Critical Basf Se
Publication of MX2014009118A publication Critical patent/MX2014009118A/es
Publication of MX351354B publication Critical patent/MX351354B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a un método para producir una proteína por medio de un microorganismo que comprende; (a) introducir una construcción de expresión en un microorganismo que comprende un promotor y un ácido nucleico que codifica para la proteína; (b) expresar la proteína en el microorganismo, en donde el microorganismo pertenece a la especie Bacillus pumilus; y en donde el microorganismo es de esporulación inhibida como un resultado dela supresión del gen spoIV (yqfD) o partes del mismo.
MX2014009118A 2012-01-31 2013-01-29 Método de expresión. MX351354B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012201297A DE102012201297A1 (de) 2012-01-31 2012-01-31 Expressionsverfahren
PCT/EP2013/051656 WO2013113689A1 (de) 2012-01-31 2013-01-29 Expressionsverfahren

Publications (2)

Publication Number Publication Date
MX2014009118A MX2014009118A (es) 2015-03-03
MX351354B true MX351354B (es) 2017-10-11

Family

ID=47683703

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009118A MX351354B (es) 2012-01-31 2013-01-29 Método de expresión.

Country Status (15)

Country Link
US (1) US9725704B2 (es)
EP (1) EP2809816B9 (es)
JP (1) JP6335793B2 (es)
KR (1) KR20150000876A (es)
CN (1) CN104471066B (es)
BR (1) BR112014018534A8 (es)
CA (1) CA2862731A1 (es)
DE (1) DE102012201297A1 (es)
DK (1) DK2809816T3 (es)
ES (1) ES2703753T3 (es)
IN (1) IN2014DN07148A (es)
MX (1) MX351354B (es)
PL (1) PL2809816T3 (es)
RU (1) RU2644199C2 (es)
WO (1) WO2013113689A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (de) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
DE102012215107A1 (de) 2012-08-24 2014-02-27 Basf Se Festes Geschirrspülmittel mit verbesserter Proteaseleistung
CN105483100B (zh) * 2016-01-13 2019-08-13 福建农林大学 一种诱导海洋微生物发酵产κ-卡拉胶酶的组合诱导物
WO2020113365A1 (zh) * 2018-12-03 2020-06-11 清华大学 一种高产脂肽的基因工程菌及其应用
US20220186200A1 (en) * 2019-03-11 2022-06-16 Basf Se Amylases and methods for making and using them
WO2020190993A1 (en) 2019-03-19 2020-09-24 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health
CN111378596B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株耐酸且兼性厌氧的锰氧化细菌及其应用
CN111378597B (zh) * 2020-01-17 2022-07-12 合肥工业大学 一株可用于脱锰的锰氧化细菌及其应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787790B2 (ja) * 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 新規な遺伝子ｄｎａおよびアルカリ性プロテア−ゼの製造法
DK0493398T3 (da) 1989-08-25 2000-05-22 Henkel Research Corp Alkalisk, proteolytisk enzym og fremgangsmåde til fremstilling deraf
EP0501765A1 (en) * 1991-03-01 1992-09-02 Takeda Chemical Industries, Ltd. Method of producing D-ribose
EP0551408B2 (en) 1991-06-11 2012-08-15 Genencor International, Inc. Detergent compositions containing cellulase compositions deficient in cbh i type components
US20030054534A1 (en) * 1992-07-02 2003-03-20 Novozymes A/S DNA constructs and methods of producing cellulytic enzymes
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
DE69535736T2 (de) 1994-02-24 2009-04-30 Henkel Ag & Co. Kgaa Verbesserte enzyme und diese enthaltene detergentien
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
ATE315083T1 (de) 1995-03-17 2006-02-15 Novozymes As Neue endoglukanase
ES2525677T3 (es) 1995-10-17 2014-12-29 Ab Enzymes Oy Celulasas, genes que las codifican y usos de las mismas
CN101085985B (zh) 1996-09-17 2012-05-16 诺沃奇梅兹有限公司 纤维素酶变体
JP4068155B2 (ja) * 1996-11-18 2008-03-26 ノボザイムス，インコーポレイティド バシラス細胞のサーファクチン突然変異株におけるポリペプチドの製造方法
US5958728A (en) * 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
WO2000053778A1 (fr) * 1999-03-08 2000-09-14 Showa Sangyo Co., Ltd. Promoteurs
CN1234854C (zh) 1999-03-31 2006-01-04 诺维信公司 具有碱性α－淀粉酶活性的多肽以及编码该多肽的核酸
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
DE60143086D1 (de) 2000-08-04 2010-10-28 Genencor Int Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
ES2521615T3 (es) 2001-06-06 2014-11-13 Novozymes A/S Endo-beta-1,4-glucanasa
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
US20040072354A1 (en) * 2001-06-29 2004-04-15 Bedzyk Laura A. Natural promoters for gene expression and metabolic monitoring in bacillus species
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
KR100879908B1 (ko) * 2001-12-21 2009-01-21 노보자임스 바이오폴리머 에이/에스 재조합체 숙주 세포에서 히알루로난의 제조 방법
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
CN103333870A (zh) 2003-12-03 2013-10-02 丹尼斯科美国公司 过水解酶
EP2287179A3 (en) * 2004-01-09 2011-05-18 Novozymes A/S Increased bacillus YweA expression
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
CN100372937C (zh) * 2005-07-21 2008-03-05 复旦大学 短小芽孢杆菌表达系统
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
DE102006032507A1 (de) * 2006-07-12 2008-01-17 Henkel Kgaa UV-sensitive Sicherheitsstämme für die biotechnolgische Produktion
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
EP2196533B9 (en) * 2007-08-10 2014-10-29 Yoshichika Kameyama Novel microorganism having gastric juice promoting action and composition secreted by the same
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus
CN101918551B (zh) * 2008-01-04 2013-11-20 天野酶株式会社 草酸脱羧酶的生产中利用的重组表达质粒载体及重组菌、以及重组草酸脱羧酶的生产方法
DE102008052529A1 (de) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expressionsverstärkte Nukleinsäuren

Also Published As

Publication number Publication date
RU2014134728A (ru) 2016-03-27
ES2703753T3 (es) 2019-03-12
PL2809816T3 (pl) 2019-04-30
CN104471066B (zh) 2019-09-03
JP6335793B2 (ja) 2018-05-30
KR20150000876A (ko) 2015-01-05
US20140370569A1 (en) 2014-12-18
RU2644199C2 (ru) 2018-02-08
DK2809816T3 (en) 2019-01-28
WO2013113689A1 (de) 2013-08-08
JP2015505463A (ja) 2015-02-23
CA2862731A1 (en) 2013-08-08
EP2809816A1 (de) 2014-12-10
IN2014DN07148A (es) 2015-04-24
BR112014018534A2 (pt) 2018-09-04
EP2809816B1 (de) 2018-10-03
US9725704B2 (en) 2017-08-08
BR112014018534A8 (pt) 2019-01-29
DE102012201297A1 (de) 2013-08-01
CN104471066A (zh) 2015-03-25
EP2809816B9 (de) 2019-02-27
MX2014009118A (es) 2015-03-03

Similar Documents

Publication Publication Date Title
MX351354B (es) Método de expresión.
IN2014DN09575A (es)
MX2014003212A (es) Microorganismos y metodos para producir alquenos.
WO2012173659A3 (en) Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
MX2015001780A (es) Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
GB2492256A (en) Microorganism production of high-valve chemical products, and related compositions, methods and systems
WO2015010103A3 (en) Microorganisms and methods for the production of fatty acids and fatty acid derived products
EA201591043A1 (ru) Способ ферментации
MX357484B (es) Cepa con deficit de amilasa.
PH12017502240A1 (en) Recombinant microorganism for improved production of alanine
MX2018007325A (es) Promotor de catalasa modificado bidireccional de bacilo.
MY186947A (en) Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways
MY171760A (en) Microorganisms and methods for the production of caprolactone
MY172080A (en) Microorganisms having putrescine productivity and process for producing putrescine using the same
SA515360688B1 (ar) السلالة البكتيرية رودوكوكوس أيثوريفورنز VKM Ac-2610D المنتجة النيتريل هيدراتاز، طريقة لزراعتها وطريقة لإنتاج الأكريلاميد
MY182946A (en) Production of organic acids by fermentation at low ph
AR093330A1 (es) Metodo para la remocion de adn
MY180352A (en) Recombinant methanotrophic bacterium and a method of production of succinic acid from methane or biogas thereof
WO2012177983A3 (en) Microorganisms for producing ethylene glycol and methods related thereto
MY180565A (en) Apparatus and method for separating and refining product manufactured by microbial fermentation by using adsorbent
MY181342A (en) Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
MX348766B (es) Metodo de expresion.
MY193588A (en) Improved microorganisms for succinic acid production
WO2017051437A3 (en) A nucleotide sequence encoding 7-dehydrocholesterol reductase, recombinant constructs and recombinant organisms comprising said sequence
MX353828B (es) Composicion que comprende acidos nucleicos de sistemas biologicos parasiticos, patogenicos o de malezas para inhibir y/o controlar el crecimiento de dichos sistemas.

Legal Events

Date Code Title Description
FG Grant or registration