IN2014DN07148A - - Google Patents

Info

Publication number
IN2014DN07148A
IN2014DN07148A IN7148DEN2014A IN2014DN07148A IN 2014DN07148 A IN2014DN07148 A IN 2014DN07148A IN 7148DEN2014 A IN7148DEN2014 A IN 7148DEN2014A IN 2014DN07148 A IN2014DN07148 A IN 2014DN07148A
Authority
IN
India
Prior art keywords
protein
expression construct
aim
microorganism
promoter
Prior art date
Application number
Inventor
Tobias Küppers
Victoria Steffen
Renée Charlott Eichstädt
Stefan Evers
Karl Heinz Maurer
Johannes Bongaerts
Hendrik Hellmuth
Thomas Weber
Timothy OCONNELL
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47683703&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IN2014DN07148(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Basf Se filed Critical Basf Se
Publication of IN2014DN07148A publication Critical patent/IN2014DN07148A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The aim of the invention is to increase the product yield of protein in a microbial fermentation process. Said aim is achieved by means of a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which expression construct comprises a promoter and a nucleic acid that codes for the protein and the protein is expressed in said expression construct.
IN7148DEN2014 2012-01-31 2013-01-29 IN2014DN07148A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102012201297A DE102012201297A1 (en) 2012-01-31 2012-01-31 expression methods
PCT/EP2013/051656 WO2013113689A1 (en) 2012-01-31 2013-01-29 Expression method

Publications (1)

Publication Number Publication Date
IN2014DN07148A true IN2014DN07148A (en) 2015-04-24

Family

ID=47683703

Family Applications (1)

Application Number Title Priority Date Filing Date
IN7148DEN2014 IN2014DN07148A (en) 2012-01-31 2013-01-29

Country Status (15)

Country Link
US (1) US9725704B2 (en)
EP (1) EP2809816B9 (en)
JP (1) JP6335793B2 (en)
KR (1) KR20150000876A (en)
CN (1) CN104471066B (en)
BR (1) BR112014018534A8 (en)
CA (1) CA2862731A1 (en)
DE (1) DE102012201297A1 (en)
DK (1) DK2809816T3 (en)
ES (1) ES2703753T3 (en)
IN (1) IN2014DN07148A (en)
MX (1) MX351354B (en)
PL (1) PL2809816T3 (en)
RU (1) RU2644199C2 (en)
WO (1) WO2013113689A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102011118032A1 (en) 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expression vectors for improved protein secretion
DE102012215107A1 (en) 2012-08-24 2014-02-27 Basf Se Solid dishwashing detergent with improved protease performance
CN105483100B (en) * 2016-01-13 2019-08-13 福建农林大学 A kind of induction marine microorganism fermentation produces the combination inducer of kappa-carrageenan enzyme
WO2020113365A1 (en) * 2018-12-03 2020-06-11 清华大学 Genetically engineered bacterium producing high yield of lipopeptides and use thereof
US20220186200A1 (en) * 2019-03-11 2022-06-16 Basf Se Amylases and methods for making and using them
WO2020190993A1 (en) 2019-03-19 2020-09-24 Bayer Cropscience Lp Fusion proteins, recombinant bacteria, and exosporium fragments for pest control and plant health
CN111378596B (en) * 2020-01-17 2022-07-12 合肥工业大学 Acid-resistant and facultative anaerobic manganese oxidizing bacterium and application thereof
CN111378597B (en) * 2020-01-17 2022-07-12 合肥工业大学 Manganese oxidizing bacterium capable of being used for demanganization and application thereof

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787790B2 (en) * 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 METHOD FOR PRODUCING NOVEL GENE DNA AND ALKALINE PROTEASE
DK0493398T3 (en) 1989-08-25 2000-05-22 Henkel Research Corp Alkaline, proteolytic enzyme and process for its preparation
EP0501765A1 (en) * 1991-03-01 1992-09-02 Takeda Chemical Industries, Ltd. Method of producing D-ribose
EP0551408B2 (en) 1991-06-11 2012-08-15 Genencor International, Inc. Detergent compositions containing cellulase compositions deficient in cbh i type components
US20030054534A1 (en) * 1992-07-02 2003-03-20 Novozymes A/S DNA constructs and methods of producing cellulytic enzymes
GB2279955B (en) * 1993-07-15 1998-02-18 Solvay Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
DE69535736T2 (en) 1994-02-24 2009-04-30 Henkel Ag & Co. Kgaa IMPROVED ENZYMES AND DETERGENTS CONTAINED THEREOF
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
ATE315083T1 (en) 1995-03-17 2006-02-15 Novozymes As NEW ENDOGLUCANASE
ES2525677T3 (en) 1995-10-17 2014-12-29 Ab Enzymes Oy Cellulases, genes that encode them and their uses
CN101085985B (en) 1996-09-17 2012-05-16 诺沃奇梅兹有限公司 Cellulase variants
JP4068155B2 (en) * 1996-11-18 2008-03-26 ノボザイムス，インコーポレイティド Method for producing a polypeptide in a surfactin mutant of Bacillus cells
US5958728A (en) * 1996-11-18 1999-09-28 Novo Nordiskbiotech, Inc. Methods for producing polypeptides in mutants of bacillus cells
DE19713852A1 (en) 1997-04-04 1998-10-08 Henkel Kgaa Activators for peroxygen compounds in detergents and cleaning agents
US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
WO2000053778A1 (en) * 1999-03-08 2000-09-14 Showa Sangyo Co., Ltd. Promoters
CN1234854C (en) 1999-03-31 2006-01-04 诺维信公司 Polypeptides having alkaline alpha-amylase activity and uncleic acids encoding same
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
DE60143086D1 (en) 2000-08-04 2010-10-28 Genencor Int MUTATED TRICHODERMA REESEI EGIII CELLULASES, DNA CORDATING THEREOF, AND METHOD FOR THE PRODUCTION THEREOF
DE10121463A1 (en) * 2001-05-02 2003-02-27 Henkel Kgaa New alkaline protease variants and washing and cleaning agents containing these new alkaline protease variants
ES2521615T3 (en) 2001-06-06 2014-11-13 Novozymes A/S Endo-beta-1,4-glucanase
DE10131441A1 (en) 2001-06-29 2003-01-30 Henkel Kgaa A new group of alpha amylases and a method for identifying and obtaining new alpha amylases
US20040072354A1 (en) * 2001-06-29 2004-04-15 Bedzyk Laura A. Natural promoters for gene expression and metabolic monitoring in bacillus species
DE10163748A1 (en) 2001-12-21 2003-07-17 Henkel Kgaa New glycosyl hydrolases
KR100879908B1 (en) * 2001-12-21 2009-01-21 노보자임스 바이오폴리머 에이/에스 Methods for producing hyaluronan in a recombinant host cell
DE10260903A1 (en) 2002-12-20 2004-07-08 Henkel Kgaa New perhydrolases
CN103333870A (en) 2003-12-03 2013-10-02 丹尼斯科美国公司 Perhydrolase enzyme
EP2287179A3 (en) * 2004-01-09 2011-05-18 Novozymes A/S Increased bacillus YweA expression
DE102004029475A1 (en) 2004-06-18 2006-01-26 Henkel Kgaa New enzymatic bleaching system
CN100372937C (en) * 2005-07-21 2008-03-05 复旦大学 Bacillus pumilus expression system
DE102006038448A1 (en) 2005-12-28 2008-02-21 Henkel Kgaa Enzyme-containing cleaning agent
DE102006022224A1 (en) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin from Bacillus pumilus and detergents and cleaners containing this new subtilisin
DE102006032507A1 (en) * 2006-07-12 2008-01-17 Henkel Kgaa UV-sensitive safety strains for biotechnological production
DE102007003143A1 (en) 2007-01-16 2008-07-17 Henkel Kgaa New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease
EP2196533B9 (en) * 2007-08-10 2014-10-29 Yoshichika Kameyama Novel microorganism having gastric juice promoting action and composition secreted by the same
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus
CN101918551B (en) * 2008-01-04 2013-11-20 天野酶株式会社 Recombinant expression plasmid vector and recombinant strain to be used in producing oxalate decarboxylase, and method of producing recombinant oxalate decarboxylase
DE102008052529A1 (en) * 2008-10-21 2010-04-22 Henkel Ag & Co. Kgaa Expression-enhanced nucleic acids

Also Published As

Publication number Publication date
RU2014134728A (en) 2016-03-27
ES2703753T3 (en) 2019-03-12
PL2809816T3 (en) 2019-04-30
CN104471066B (en) 2019-09-03
JP6335793B2 (en) 2018-05-30
KR20150000876A (en) 2015-01-05
US20140370569A1 (en) 2014-12-18
RU2644199C2 (en) 2018-02-08
DK2809816T3 (en) 2019-01-28
WO2013113689A1 (en) 2013-08-08
JP2015505463A (en) 2015-02-23
CA2862731A1 (en) 2013-08-08
EP2809816A1 (en) 2014-12-10
MX351354B (en) 2017-10-11
BR112014018534A2 (en) 2018-09-04
EP2809816B1 (en) 2018-10-03
US9725704B2 (en) 2017-08-08
BR112014018534A8 (en) 2019-01-29
DE102012201297A1 (en) 2013-08-01
CN104471066A (en) 2015-03-25
EP2809816B9 (en) 2019-02-27
MX2014009118A (en) 2015-03-03

Similar Documents

Publication Publication Date Title
IN2014DN07148A (en)
MX2015001780A (en) Microorganisms and methods for the production of fatty acids and fatty acid derived products.
MX2014003212A (en) Microorganisms and methods for producing alkenes.
IN2014DN09575A (en)
GB2492256A (en) Microorganism production of high-valve chemical products, and related compositions, methods and systems
WO2015010103A3 (en) Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2012173659A3 (en) Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
MX356002B (en) Method and system for processing of aquatic species.
EA201591043A1 (en) METHOD OF ENZYMATION
MX357484B (en) Amylase-deficient strain.
MY171760A (en) Microorganisms and methods for the production of caprolactone
MY186947A (en) Method for the production of l-serine using genetically engineered microorganisms deficient in serine degradation pathways
MX2018007325A (en) Modified bidirectional catalase promoter from bacillus.
SA515360688B1 (en) Bacterial strain rhodococcus aetherivorans VKM Ac-2610D producing nitrile hydratase, method of its cultivation and method for producing acrylamide
MX348547B (en) A novel process of cultivating bacteria for yield improvement of capsular polyoses.
MY172080A (en) Microorganisms having putrescine productivity and process for producing putrescine using the same
EA201400076A1 (en) METHOD FOR CREATING ORGANIC MATERIAL
MY193225A (en) Methods for continuous production of products from microorganisms
AR093330A1 (en) METHOD FOR DNA REMOVAL
MY180565A (en) Apparatus and method for separating and refining product manufactured by microbial fermentation by using adsorbent
WO2012177983A3 (en) Microorganisms for producing ethylene glycol and methods related thereto
MY181342A (en) Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
IN2014CN00793A (en)
MX348766B (en) Expression method.
MX2016008850A (en) Method of increasing biomass and lipid content in a micro-organism and a genetically modified micro-organism exhibiting enhanced autophagy.