AR093330A1 - METHOD FOR DNA REMOVAL - Google Patents

METHOD FOR DNA REMOVAL

Info

Publication number
AR093330A1
AR093330A1 ARP130104004A ARP130104004A AR093330A1 AR 093330 A1 AR093330 A1 AR 093330A1 AR P130104004 A ARP130104004 A AR P130104004A AR P130104004 A ARP130104004 A AR P130104004A AR 093330 A1 AR093330 A1 AR 093330A1
Authority
AR
Argentina
Prior art keywords
fermentation broth
dna removal
interest
protein
microorganism
Prior art date
Application number
ARP130104004A
Other languages
Spanish (es)
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47172448&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR093330(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes As filed Critical Novozymes As
Publication of AR093330A1 publication Critical patent/AR093330A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para la remoción del ADN residual de un caldo de fermentación que comprende una proteína de interés y un microorganismo que produce la proteína de interés, comprendiendo dicho método: a) agregar un cloruro de polialuminio al caldo de fermentación, y b) separar el microorganismo floculado del caldo de fermentación.A method for removing residual DNA from a fermentation broth comprising a protein of interest and a microorganism that produces the protein of interest, said method comprising: a) adding a polyaluminium chloride to the fermentation broth, and b) separating the microorganism flocculation of the fermentation broth.

ARP130104004A 2012-11-01 2013-11-01 METHOD FOR DNA REMOVAL AR093330A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12190937 2012-11-01

Publications (1)

Publication Number Publication Date
AR093330A1 true AR093330A1 (en) 2015-06-03

Family

ID=47172448

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130104004A AR093330A1 (en) 2012-11-01 2013-11-01 METHOD FOR DNA REMOVAL

Country Status (6)

Country Link
US (2) US20150291656A1 (en)
EP (1) EP2914611B1 (en)
CN (1) CN104797590A (en)
AR (1) AR093330A1 (en)
DK (1) DK2914611T3 (en)
WO (1) WO2014068083A1 (en)

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WO2008079280A1 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US20100190963A1 (en) 2008-12-16 2010-07-29 Millipore Corporation Stirred Tank Reactor And Method
BR112012028977B1 (en) 2010-05-17 2020-05-05 Emd Millipore Corp biomolecule purification method by means of stimulus-responsive polymers
SG11202101113YA (en) * 2018-08-30 2021-04-29 Glaxosmithkline Ip Dev Ltd Methods of detecting nucleic acid
EP3926039A1 (en) 2020-06-17 2021-12-22 AB Enzymes GmbH Use of a nuclease for reducing the viscosity and/or preventing an increase in viscosity of a fermentation broth
WO2023118565A1 (en) * 2021-12-23 2023-06-29 Novozymes A/S Reduction of residual dna in microbial fermentation products

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Also Published As

Publication number Publication date
US20150291656A1 (en) 2015-10-15
DK2914611T3 (en) 2018-12-10
EP2914611A1 (en) 2015-09-09
CN104797590A (en) 2015-07-22
EP2914611B1 (en) 2018-08-29
US20180030087A1 (en) 2018-02-01
WO2014068083A1 (en) 2014-05-08

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