CA2831473A1 - Expression method - Google Patents

Expression method Download PDF

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Publication number
CA2831473A1
CA2831473A1 CA2831473A CA2831473A CA2831473A1 CA 2831473 A1 CA2831473 A1 CA 2831473A1 CA 2831473 A CA2831473 A CA 2831473A CA 2831473 A CA2831473 A CA 2831473A CA 2831473 A1 CA2831473 A1 CA 2831473A1
Authority
CA
Canada
Prior art keywords
microorganism
protein
protease
bacillus
auxiliary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2831473A
Other languages
English (en)
French (fr)
Inventor
Lukas MAKSYM
Ramona KNAB
Stefan Evers
Karl-Heinz Maurer
Johannes Bongaerts
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Publication of CA2831473A1 publication Critical patent/CA2831473A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
CA2831473A 2011-04-13 2012-04-05 Expression method Abandoned CA2831473A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102011007313.2 2011-04-13
DE102011007313A DE102011007313A1 (de) 2011-04-13 2011-04-13 Expressionsverfahren
PCT/EP2012/056262 WO2012139964A1 (de) 2011-04-13 2012-04-05 Expressionsverfahren

Publications (1)

Publication Number Publication Date
CA2831473A1 true CA2831473A1 (en) 2012-10-18

Family

ID=45952535

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2831473A Abandoned CA2831473A1 (en) 2011-04-13 2012-04-05 Expression method

Country Status (14)

Country Link
US (1) US9663798B2 (en22)
EP (1) EP2697376B1 (en22)
JP (2) JP6099627B2 (en22)
KR (1) KR20140019436A (en22)
CN (1) CN103608457A (en22)
BR (1) BR112013025951A2 (en22)
CA (1) CA2831473A1 (en22)
DE (1) DE102011007313A1 (en22)
DK (1) DK2697376T3 (en22)
ES (1) ES2639114T3 (en22)
MX (1) MX348766B (en22)
PL (1) PL2697376T3 (en22)
RU (1) RU2642324C2 (en22)
WO (1) WO2012139964A1 (en22)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104059869A (zh) * 2014-06-20 2014-09-24 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用
US9803183B2 (en) 2011-05-31 2017-10-31 Basf Se Expression vectors for an improved protein secretion

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
KR102112240B1 (ko) * 2018-09-28 2020-05-18 씨제이제일제당 주식회사 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability

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DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
EP0493398B1 (en) 1989-08-25 1999-12-08 Henkel Research Corporation Alkaline proteolytic enzyme and method of production
ES2144401T5 (es) 1991-06-11 2012-11-27 Genencor International, Inc. Composiciones detergentes que contienen composiciones de celulasa deficientes en componentes de tipo CBH I
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
DE69535736T2 (de) * 1994-02-24 2009-04-30 Henkel Ag & Co. Kgaa Verbesserte enzyme und diese enthaltene detergentien
CN1182451A (zh) 1995-03-17 1998-05-20 诺沃挪第克公司 新的内切葡聚糖酶
DK0857216T3 (en) 1995-10-17 2014-12-15 Ab Enzymes Oy Cellulases, GENES ENCODING THEM AND USES THEREOF
CN100362100C (zh) 1996-09-17 2008-01-16 诺沃奇梅兹有限公司 纤维素酶变体
DE19713852A1 (de) 1997-04-04 1998-10-08 Henkel Kgaa Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln
ES2532606T3 (es) 1999-03-31 2015-03-30 Novozymes A/S Polipéptidos con actividad de alfa-amilasa alcalina y ácidos nucleicos que los codifican
EP1240525A2 (en) 1999-12-23 2002-09-18 PHARMACIA & UPJOHN COMPANY Sodium channels as targets for amyloid beta
DK1305432T3 (da) 2000-08-04 2010-12-06 Genencor Int Muterede trichoderma reesei EGIII-cellulaser, DNA, der koder for sådanne EGIII-sammensætninger og fremgangsmåder til at frembringe samme
CN1633496A (zh) 2001-06-06 2005-06-29 诺和酶股份有限公司 内切－β－1,4－葡聚糖酶
DE10131441A1 (de) 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
DE10163748A1 (de) 2001-12-21 2003-07-17 Henkel Kgaa Neue Glykosylhydrolasen
DE60330346D1 (de) 2002-01-16 2010-01-14 Genencor Int Mehrfachsubstituierte proteasevarianten
DE10260903A1 (de) 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
DE10309555A1 (de) * 2003-03-04 2004-09-23 Henkel Kgaa Faktor PrfB (Peptide chain release factor B) aus Bacillus licheniformis und Steigerung der Proteinbildungsrate durch Erhöhung der PrfB-Aktivität
MXPA06005652A (es) 2003-12-03 2006-08-17 Genencor Int Perhidrolasa.
DE102004013843A1 (de) * 2004-03-20 2005-10-13 Degussa Ag Expression von Nitrilhydratasen im Zwei-Vektor-Expressionssystem
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
US20080199443A1 (en) 2005-06-09 2008-08-21 The Govt. Of The U.S.A. As Represented By The Secretary, Dept. Of Health And Human Services Bone Morphogenetic Variants, Compositions and Methods of Treatment
DE102006038448A1 (de) 2005-12-28 2008-02-21 Henkel Kgaa Enzym-haltiges Reinigungsmittel
JP2009534022A (ja) * 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ 卵のシミに対する洗浄能力が向上したサビナーゼ変異体
DE102006022224A1 (de) 2006-05-11 2007-11-15 Henkel Kgaa Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin
EP1873251A1 (en) * 2006-06-29 2008-01-02 Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus Expression vector(s) for enhanced expression of a protein of interest in eukaryotic or prokaryotic host cells
US20080090747A1 (en) * 2006-07-18 2008-04-17 Pieter Augustinus Protease variants active over a broad temperature range
DE102007003143A1 (de) 2007-01-16 2008-07-17 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP5629584B2 (ja) 2007-12-21 2014-11-19 ダニスコ・ユーエス・インク バチルス内での促進されたタンパク質生成

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9803183B2 (en) 2011-05-31 2017-10-31 Basf Se Expression vectors for an improved protein secretion
US10494622B2 (en) 2011-05-31 2019-12-03 Basf Se Expression vectors with promoter and nucleic acid
US11046961B2 (en) 2011-05-31 2021-06-29 Basf Se Expression vectors with promoter and nucleic acid
CN104059869A (zh) * 2014-06-20 2014-09-24 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用
CN104059869B (zh) * 2014-06-20 2016-07-06 淮海工学院 一株海洋解淀粉芽孢杆菌及其培养方法和应用

Also Published As

Publication number Publication date
MX2013011816A (es) 2013-10-25
EP2697376B1 (de) 2017-05-31
RU2013150276A (ru) 2015-05-20
RU2642324C2 (ru) 2018-01-24
ES2639114T3 (es) 2017-10-25
JP6099627B2 (ja) 2017-03-22
WO2012139964A1 (de) 2012-10-18
KR20140019436A (ko) 2014-02-14
US20140220628A1 (en) 2014-08-07
PL2697376T3 (pl) 2017-10-31
BR112013025951A2 (pt) 2017-08-01
DK2697376T3 (en) 2017-09-18
JP2017079768A (ja) 2017-05-18
JP2014511702A (ja) 2014-05-19
MX348766B (es) 2017-06-28
DE102011007313A1 (de) 2012-10-18
EP2697376A1 (de) 2014-02-19
US9663798B2 (en) 2017-05-30
CN103608457A (zh) 2014-02-26

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170403

FZDE Discontinued

Effective date: 20200831