JP5991796B2 - 胚性幹細胞培養のための自動化された方法および装置 - Google Patents

胚性幹細胞培養のための自動化された方法および装置 Download PDF

Info

Publication number
JP5991796B2
JP5991796B2 JP2010515226A JP2010515226A JP5991796B2 JP 5991796 B2 JP5991796 B2 JP 5991796B2 JP 2010515226 A JP2010515226 A JP 2010515226A JP 2010515226 A JP2010515226 A JP 2010515226A JP 5991796 B2 JP5991796 B2 JP 5991796B2
Authority
JP
Japan
Prior art keywords
cells
cell
pluripotent
medium
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2010515226A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010532173A5 (https=
JP2010532173A (ja
Inventor
ナサニエル ベアーズリー，
ナサニエル ベアーズリー，
ベイト バーゲンダル，
ベイト バーゲンダル，
メーガン フィッツジェラルド，
メーガン フィッツジェラルド，
クリスティン ダイグ，
クリスティン ダイグ，
Original Assignee
セルラー ダイナミクス インターナショナル， インコーポレイテッド
セルラー ダイナミクス インターナショナル， インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セルラー ダイナミクス インターナショナル， インコーポレイテッド, セルラー ダイナミクス インターナショナル， インコーポレイテッド filed Critical セルラー ダイナミクス インターナショナル， インコーポレイテッド
Publication of JP2010532173A publication Critical patent/JP2010532173A/ja
Publication of JP2010532173A5 publication Critical patent/JP2010532173A5/ja
Application granted granted Critical
Publication of JP5991796B2 publication Critical patent/JP5991796B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
JP2010515226A 2007-06-29 2008-06-30 胚性幹細胞培養のための自動化された方法および装置 Active JP5991796B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94701307P 2007-06-29 2007-06-29
US60/947,013 2007-06-29
PCT/US2008/068814 WO2009006422A1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2014063625A Division JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Division JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置

Publications (3)

Publication Number Publication Date
JP2010532173A JP2010532173A (ja) 2010-10-07
JP2010532173A5 JP2010532173A5 (https=) 2012-07-26
JP5991796B2 true JP5991796B2 (ja) 2016-09-14

Family

ID=39672032

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2010515226A Active JP5991796B2 (ja) 2007-06-29 2008-06-30 胚性幹細胞培養のための自動化された方法および装置
JP2014063625A Pending JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Pending JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置
JP2016217785A Pending JP2017023158A (ja) 2007-06-29 2016-11-08 胚性幹細胞培養のための自動化された方法および装置

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2014063625A Pending JP2014110817A (ja) 2007-06-29 2014-03-26 胚性幹細胞培養のための自動化された方法および装置
JP2016125186A Pending JP2016168056A (ja) 2007-06-29 2016-06-24 胚性幹細胞培養のための自動化された方法および装置
JP2016217785A Pending JP2017023158A (ja) 2007-06-29 2016-11-08 胚性幹細胞培養のための自動化された方法および装置

Country Status (8)

Country Link
US (2) US8815585B2 (https=)
EP (2) EP2173863B1 (https=)
JP (4) JP5991796B2 (https=)
KR (2) KR20160005142A (https=)
AU (1) AU2008272949B2 (https=)
CA (1) CA2691793A1 (https=)
DK (2) DK3293256T3 (https=)
WO (1) WO2009006422A1 (https=)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007026353A2 (en) 2005-08-29 2007-03-08 Technion Research & Development Foundation Ltd. Media for culturing stem cells
EP2059586B1 (en) 2006-08-02 2016-07-20 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA3114827C (en) 2007-07-31 2023-09-05 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
KR101731474B1 (ko) 2008-02-21 2017-05-11 얀센 바이오테크 인코포레이티드 세포 부착, 배양 및 탈리를 위한 방법, 표면 개질 플레이트 및 조성물
US8691565B2 (en) 2008-03-05 2014-04-08 Terumo Bct, Inc. Method of reseeding adherent cells grown in a hollow fiber bioreactor system
US8828720B2 (en) 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
US9458431B2 (en) 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
US8691569B2 (en) 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
SG188918A1 (https=) 2008-03-17 2013-04-30 Agency Science Tech & Res
SG188904A1 (en) 2008-03-17 2013-04-30 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
PL2310492T3 (pl) 2008-06-30 2015-12-31 Janssen Biotech Inc Różnocowanie pluripotencjalnych komórek macierzystych
EP2295538B1 (en) * 2008-07-07 2015-10-21 Takara Bio Inc. Method for production of pluripotent stem cell
ES2584053T3 (es) 2008-11-20 2016-09-23 Janssen Biotech, Inc. Métodos y composiciones para la unión de células y cultivo en sustratos planos
ES2642070T3 (es) 2008-11-20 2017-11-15 Janssen Biotech, Inc. Cultivo de células madre pluripotentes en microportadores
BRPI0922572A2 (pt) 2008-12-17 2019-09-24 Scripps Research Inst método para cultivar células pluripotentes, cultura de células de mamífero pluripotentes, meio de cultura de célula, célula animal pluripotente isolada, e, método para aumentar a pluripotência de uma célula de mamífero.
EP2398897B1 (en) 2009-02-20 2017-06-28 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
US9150829B2 (en) 2009-03-20 2015-10-06 Agency For Science, Technoloy And Research Culture of pluripotent and multipotent cells on microcarriers
KR102058901B1 (ko) 2009-07-20 2019-12-24 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 분화
WO2011043077A1 (ja) * 2009-10-09 2011-04-14 川崎重工業株式会社 未分化多能性幹細胞の識別方法及び装置並びに自動培養方法及び装置
AU2010306627B2 (en) 2009-10-16 2014-07-17 The Scripps Research Institute Induction of pluripotent cells
ES2539487T3 (es) 2009-11-04 2015-07-01 Cellular Dynamics International, Inc. Reprogramación episómica con compuestos químicos
EP3633025B1 (en) 2009-11-12 2022-09-14 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
CA2725728C (en) * 2009-11-24 2014-01-28 University Of Connecticut Differentiation of human embryonic and induced pluripotent stem cells
SG181687A1 (en) 2009-12-23 2012-07-30 Centocor Ortho Biotech Inc Differentiation of human embryonic stem cells
EP2542667A4 (en) 2010-03-01 2013-07-31 Janssen Biotech Inc PROCESS FOR CLEANING CELLS OBTAINED FROM PLURIPOTENTIAL STEM CELLS
CA2800498C (en) 2010-03-31 2021-11-16 The Scripps Research Institute Reprogramming cells
AU2011250651B2 (en) * 2010-05-05 2015-10-08 Genea Ip Holdings Pty Limited Media and methods for cell culture
EP3399026B1 (en) 2010-06-14 2024-06-26 The Scripps Research Institute Reprogramming of cells to a new fate
JP5696144B2 (ja) 2010-06-25 2015-04-08 川崎重工業株式会社 多能性幹細胞コロニーの識別方法及び装置並びに多能性幹細胞の自動培養方法及び装置
CN103221536B (zh) 2010-08-31 2016-08-31 詹森生物科技公司 人胚胎干细胞的分化
CA2810488C (en) 2010-09-07 2025-05-06 Technion Research & Development Foundation Limited NEW PROCESSES AND CULTURE MEDIA FOR THE CULTURE OF MULTIPOTENTED STEM CELLS
WO2012087965A2 (en) 2010-12-22 2012-06-28 Fate Therapauetics, Inc. Cell culture platform for single cell sorting and enhanced reprogramming of ipscs
KR101293300B1 (ko) * 2011-04-05 2013-08-09 (주)로고스바이오시스템스 세포의 분리시기를 판정하는 방법과, 이를 이용한 세포의 계대 배양 방법 및 계대 배양 장치
WO2013058403A1 (ja) * 2011-10-21 2013-04-25 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
WO2013082509A1 (en) 2011-12-01 2013-06-06 The New York Stem Cell Foundation Automated system for producing induced pluripotent stem cells or differentiated cells
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
KR102203056B1 (ko) 2011-12-22 2021-01-14 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
AU2013271581B2 (en) 2012-06-08 2018-08-09 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
BR112015015770A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas
JP6557147B2 (ja) 2012-12-31 2019-08-07 ヤンセン バイオテツク，インコーポレーテツド Ｈｂ９調節物を用いたヒト胚性幹細胞の膵臓内分泌細胞への分化
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
US10465158B2 (en) 2013-07-12 2019-11-05 President And Fellows Of Harvard College Systems and methods for cell culture device interconnection and fluidic device interconnection
US10202568B2 (en) 2013-08-12 2019-02-12 Invivosciences Inc. Automated cell culture system and method
SG11201606934SA (en) 2014-03-04 2016-09-29 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
US10006006B2 (en) 2014-05-16 2018-06-26 Janssen Biotech, Inc. Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
WO2016028880A1 (en) 2014-08-19 2016-02-25 Cellular Dynamics International, Inc. Neural networks formed from cells derived from pluripotent stem cells
WO2016044681A1 (en) 2014-09-18 2016-03-24 North Carolina State University Mammalian lung spheroids and lung spheroid cells and uses thereof
EP3212759A1 (en) 2014-10-29 2017-09-06 Corning Incorporated Cell culture insert
WO2016069930A1 (en) 2014-10-29 2016-05-06 Corning Incorporated Microwell design and fabrication for generation of cell culture aggregates
JP6930914B2 (ja) 2014-10-29 2021-09-01 コーニング インコーポレイテッド 灌流バイオリアクタ・プラットフォーム
AU2015363008B2 (en) 2014-12-19 2021-12-09 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
EP3315604A4 (en) * 2015-06-25 2018-12-26 Kaneka Corporation Liquid injection method
WO2017007278A1 (ko) * 2015-07-09 2017-01-12 사회복지법인 삼성생명공익재단 자동 세포 배양기 및 그 배양기의 동작 방법
CN108138130A (zh) 2015-08-31 2018-06-08 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
AU2016331926B2 (en) 2015-10-01 2021-12-09 Berkeley Lights, Inc. Well-plate incubator
SG10202111851YA (en) 2015-10-16 2021-12-30 Fate Therapeutics Inc Platform for the Induction & Maintenance of Ground State Pluripotency
CN109069870B (zh) 2016-02-24 2022-04-29 洛克菲勒大学 基于胚胎细胞的用于亨廷顿氏病的治疗候选物筛选系统、模型及它们的应用
JP6943449B2 (ja) * 2016-03-21 2021-09-29 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代
DK3443073T3 (da) 2016-04-14 2024-09-02 Janssen Biotech Inc Differentiering af pluripotente stamceller til intestinale mellemtarmendodermceller
JP2018000130A (ja) * 2016-07-05 2018-01-11 株式会社Ihi 細胞培養装置
KR102711870B1 (ko) * 2016-07-19 2024-10-02 아셀라타 리미티드 현탁 내 만능줄기세포를 배양하기 위한 배양 배지
DE102016114043B3 (de) 2016-07-29 2017-08-10 Technische Universität Dresden Vorrichtung zur Isolierung von Stammzellen aus fötalen Geweben
WO2018044699A1 (en) 2016-08-27 2018-03-08 3D Biotek, Llc Bioreactor
EP3538644B1 (en) * 2016-11-10 2021-12-29 Becton, Dickinson and Company Timeline system for monitoring a culture media protocol
CA3045898A1 (en) * 2016-12-01 2018-06-07 Berkeley Lights, Inc. Well-plate incubator
US20190382706A1 (en) * 2017-02-27 2019-12-19 Koji Tanabe Somatic cell production system
CN115247129A (zh) * 2017-02-27 2022-10-28 田边刚士 细胞处理装置
EP3652290B1 (en) 2017-07-14 2022-05-04 Corning Incorporated 3d cell culture vessels for manual or automatic media exchange
JP7197557B2 (ja) 2017-07-14 2022-12-27 コーニング インコーポレイテッド 多孔性支持体を有する細胞培養槽
JP7195302B2 (ja) 2017-07-14 2022-12-23 コーニング インコーポレイテッド ３ｄ培養のための細胞培養容器及び３ｄ細胞の培養方法
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
KR102220125B1 (ko) * 2018-04-26 2021-02-25 (주)세포바이오 세포배양 자동화 장치 및 방법
WO2020013847A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Microcavity dishes with sidewall including liquid medium delivery surface
CN111065725B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 包括具有互联的壁的微板的流体装置
WO2020013845A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Cell culture vessels with stabilizer devices
US20230035127A1 (en) * 2021-07-21 2023-02-02 University Of Maryland, College Park Culture and differentiation of pluripotent stem cells

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
JPS58155087A (ja) * 1982-03-12 1983-09-14 Olympus Optical Co Ltd 細胞の自動培養装置
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
JP3246664B2 (ja) 1993-01-29 2002-01-15 ニュー ブルンズウイック サイエンティフィック カンパニー インコーポレイテッド 足場および懸濁細胞の培養方法並びにその装置
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6325114B1 (en) 2000-02-01 2001-12-04 Incyte Genomics, Inc. Pipetting station apparatus
WO2003087292A2 (en) * 2002-04-08 2003-10-23 Millenium Biologix Inc. Automated tissue engineering system
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
CN101052712A (zh) 2004-09-08 2007-10-10 威斯康星校友研究基金会 培养人胚胎干细胞
US20060198827A1 (en) * 2005-02-04 2006-09-07 Shulamit Levenberg Engineering vascularized muscle tissue
US20060199265A1 (en) * 2005-03-02 2006-09-07 Wolf Michael F Seeding implantable medical devices with cells

Also Published As

Publication number Publication date
US20130316445A1 (en) 2013-11-28
KR20100059789A (ko) 2010-06-04
DK2173863T3 (en) 2019-01-21
KR20160005142A (ko) 2016-01-13
JP2016168056A (ja) 2016-09-23
US20090029462A1 (en) 2009-01-29
DK3293256T3 (da) 2019-08-12
EP2173863B1 (en) 2018-10-10
EP3293256A1 (en) 2018-03-14
EP2173863A1 (en) 2010-04-14
JP2010532173A (ja) 2010-10-07
AU2008272949A1 (en) 2009-01-08
WO2009006422A1 (en) 2009-01-08
EP3293256B1 (en) 2019-05-22
US8815585B2 (en) 2014-08-26
JP2014110817A (ja) 2014-06-19
JP2017023158A (ja) 2017-02-02
CA2691793A1 (en) 2009-01-08
AU2008272949B2 (en) 2014-05-15

Similar Documents

Publication Publication Date Title
JP5991796B2 (ja) 胚性幹細胞培養のための自動化された方法および装置
JP7123412B2 (ja) オルガノイドを培養するための方法
JP6495281B2 (ja) オートメーション化された細胞培養システム及び方法
CN101611139B (zh) 利用微载体的产后来源的细胞的体外扩增
JP7510247B2 (ja) 細胞凝集塊を解離させる方法
EP2398897B1 (en) Methods and compositions for the differentiation of stem cells
US20100093083A1 (en) Large scale production of stem cells
CN107267442A (zh) 微载体上的多能干细胞培养
Reid [12] Cloning
JP7804327B2 (ja) バイオリアクターにおける懸濁状態での幹細胞の増殖方法
AU2014201955B2 (en) Automated method and apparatus for embryonic stem cell culture
US20250122474A1 (en) Production method for natural killer cells
JP7092309B2 (ja) 単一細胞ヒト多能性幹細胞の継代及び採取用配合物
WO2025062293A1 (en) Methods and materials for scalable 3d cellular redifferentiation
WO2025146565A1 (en) Scalable generation of human induced pluripotent stem cell derived cardiac organoids
Jing et al. Stem cell bioprocessing for regenerative medicine
HK40119737A (en) Production method for natural killer cells
CN115052966A (zh) 三维细胞培养

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110630

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20110630

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120530

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130611

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130808

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20131227

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140326

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20140326

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20140825

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20141224

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150203

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20150313

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160422

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20160524

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160624

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160816

R150 Certificate of patent or registration of utility model

Ref document number: 5991796

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D03

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D04

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250