KR20160005142A - 배아줄기세포 배양을 위한 자동화 방법 및 장치 - Google Patents

배아줄기세포 배양을 위한 자동화 방법 및 장치 Download PDF

Info

Publication number
KR20160005142A
KR20160005142A KR1020157036767A KR20157036767A KR20160005142A KR 20160005142 A KR20160005142 A KR 20160005142A KR 1020157036767 A KR1020157036767 A KR 1020157036767A KR 20157036767 A KR20157036767 A KR 20157036767A KR 20160005142 A KR20160005142 A KR 20160005142A
Authority
KR
South Korea
Prior art keywords
cells
stem cells
pluripotent stem
cell
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020157036767A
Other languages
English (en)
Korean (ko)
Inventor
나다니엘 비어즐리
파이트 베르겐달
메건 피츠제럴드
크리스틴 다이그
Original Assignee
셀룰러 다이내믹스 인터내셔널, 인코포레이티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 filed Critical 셀룰러 다이내믹스 인터내셔널, 인코포레이티드
Publication of KR20160005142A publication Critical patent/KR20160005142A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/07—Dosage or metering devices therefore
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Virology (AREA)
  • Transplantation (AREA)
KR1020157036767A 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치 Ceased KR20160005142A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94701307P 2007-06-29 2007-06-29
US60/947,013 2007-06-29
PCT/US2008/068814 WO2009006422A1 (en) 2007-06-29 2008-06-30 Automated method and apparatus for embryonic stem cell culture

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020107002003A Division KR20100059789A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치

Publications (1)

Publication Number Publication Date
KR20160005142A true KR20160005142A (ko) 2016-01-13

Family

ID=39672032

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020157036767A Ceased KR20160005142A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치
KR1020107002003A Ceased KR20100059789A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020107002003A Ceased KR20100059789A (ko) 2007-06-29 2008-06-30 배아줄기세포 배양을 위한 자동화 방법 및 장치

Country Status (8)

Country Link
US (2) US8815585B2 (https=)
EP (2) EP3293256B1 (https=)
JP (4) JP5991796B2 (https=)
KR (2) KR20160005142A (https=)
AU (1) AU2008272949B2 (https=)
CA (1) CA2691793A1 (https=)
DK (2) DK2173863T3 (https=)
WO (1) WO2009006422A1 (https=)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
DK2185693T3 (da) 2007-07-31 2019-09-23 Lifescan Inc Differentiering af humane embryoniske stamceller
JP5733986B2 (ja) 2008-02-21 2015-06-10 ヤンセン バイオテツク，インコーポレーテツド 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物
US8691569B2 (en) 2008-03-17 2014-04-08 Agency For Science, Technology And Research Microcarriers for stem cell culture
US9458431B2 (en) 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
JP2011514169A (ja) 2008-03-17 2011-05-06 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 幹細胞培養のためのマイクロキャリア
US8828720B2 (en) 2008-03-17 2014-09-09 Agency For Science, Technology And Research Microcarriers for stem cell culture
MX2010010165A (es) 2008-03-17 2010-11-25 Scripps Research Inst Procedimientos quimicos y geneticos combinados para generacion de celulas madre pluripotentes inducidas.
EP2942392B1 (en) 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CN102144027B (zh) * 2008-07-07 2016-04-06 宝生物工程株式会社 生产多能干细胞的方法
KR101687344B1 (ko) 2008-11-20 2016-12-16 얀센 바이오테크 인코포레이티드 평면 기재상의 세포 부착 및 배양을 위한 방법 및 조성물
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
WO2010077955A1 (en) 2008-12-17 2010-07-08 The Scripps Research Institute Generation and maintenance of stem cells
WO2010096746A1 (en) 2009-02-20 2010-08-26 Cellular Dynamics International, Inc. Methods and compositions for the differentiation of stem cells
JP2012520672A (ja) * 2009-03-20 2012-09-10 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ マイクロキャリアー上の多能性（ｐｌｕｒｉｐｏｔｅｎｔ）および多分化能（ｍｕｌｔｉｐｏｔｅｎｔ）細胞の培養
CN102482643B (zh) 2009-07-20 2016-06-29 詹森生物科技公司 人胚胎干细胞的分化
WO2011043077A1 (ja) * 2009-10-09 2011-04-14 川崎重工業株式会社 未分化多能性幹細胞の識別方法及び装置並びに自動培養方法及び装置
CA2777720C (en) * 2009-10-16 2020-10-06 The Scripps Research Institute Induction of pluripotent cells
WO2011056971A2 (en) * 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
US9018010B2 (en) 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
CA2725728C (en) * 2009-11-24 2014-01-28 University Of Connecticut Differentiation of human embryonic and induced pluripotent stem cells
SG10201408552YA (en) 2009-12-23 2015-02-27 Janssen Biotech Inc Differentiation of human embryonic stem cells
PH12012501686A1 (en) 2010-03-01 2020-10-19 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
CN102959076B (zh) 2010-03-31 2015-09-16 斯克里普斯研究所 重编程细胞
US20130071927A1 (en) * 2010-05-05 2013-03-21 Sydney Ivf Limited Media and methods for cell culture
EP2566951B1 (en) 2010-05-05 2020-03-04 Terumo BCT, Inc. Method of reseeding adherent cells grown in a hollow fiber bioreactor system
WO2011159726A2 (en) 2010-06-14 2011-12-22 The Scripps Research Institute Reprogramming of cells to a new fate
WO2011161962A1 (ja) 2010-06-25 2011-12-29 川崎重工業株式会社 多能性幹細胞コロニーの識別方法及び装置並びに多能性幹細胞の自動培養方法及び装置
EP3372672A1 (en) 2010-08-31 2018-09-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP6283223B2 (ja) 2010-09-07 2018-02-21 テクニオン リサーチ アンド ディベロップメント ファウンデーション リミテッド 多能性幹細胞を培養するための新規な方法および培養培地
EP2655601A4 (en) 2010-12-22 2014-09-10 Fate Therapeutics Inc CELL CULTURAL PLATFORM FOR INDIVIDUAL CELL SIZING AND IMPROVED RESOLUTION OF IPSCS
KR101293300B1 (ko) * 2011-04-05 2013-08-09 (주)로고스바이오시스템스 세포의 분리시기를 판정하는 방법과, 이를 이용한 세포의 계대 배양 방법 및 계대 배양 장치
WO2013058403A1 (ja) * 2011-10-21 2013-04-25 国立大学法人京都大学 層流による多能性維持単一分散細胞培養法
ES2773863T3 (es) 2011-12-01 2020-07-15 New York Stem Cell Found Inc Sistema automatizado para la producción de células madre pluripotentes inducidas o células diferenciadas
US10428309B2 (en) 2011-12-01 2019-10-01 New York Stem Cell Foundation, Inc. Systems and methods for producing stem cells and differentiated cells
FI2794857T3 (fi) 2011-12-22 2026-03-25 Janssen Biotech Inc Ihmisen alkion kantasolujen erilaistaminen yksittäisiksi hormonaalisiksi insuliinipositiivisiksi soluiksi
CN102604894B (zh) 2012-02-29 2014-07-30 中国科学院广州生物医药与健康研究院 用于制备神经干细胞的培养基及其用途
EP3450544A1 (en) 2012-06-08 2019-03-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endodrine cells
ES2837763T3 (es) 2012-12-31 2021-07-01 Janssen Biotech Inc Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas
DK2938723T3 (da) 2012-12-31 2023-02-20 Janssen Biotech Inc Differentiering af humane embryonale stamceller til pancreatiske endokrine celler under anvendelse af hb9-regulatorer
BR112015015714A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
US9790465B2 (en) 2013-04-30 2017-10-17 Corning Incorporated Spheroid cell culture well article and methods thereof
CA2917889C (en) 2013-07-12 2023-10-03 President And Fellows Of Harvard College Systems and methods for cell culture device interconnection and fluidic device interconnection
EP3033414A4 (en) * 2013-08-12 2017-03-22 Invivosciences Inc. Automated cell culture system and method
CA2941004A1 (en) 2014-03-04 2015-09-11 Peter Flynn Improved reprogramming methods and cell culture platforms
WO2015175307A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
AU2015305515B2 (en) 2014-08-19 2020-12-03 FUJIFILM Cellular Dynamics, Inc. Neural networks formed from cells derived from pluripotent stem cells
US20170296595A1 (en) 2014-09-18 2017-10-19 North Carolina State University Mammalian Lung Spheroids and Lung Spheroid Cells and Uses Thereof
JP2017532971A (ja) 2014-10-29 2017-11-09 コーニング インコーポレイテッド 細胞培養集合体を生成するためのマイクロウェル設計および製造
EP3212760A1 (en) 2014-10-29 2017-09-06 Corning Incorporated Perfusion bioreactor platform
EP3212759A1 (en) 2014-10-29 2017-09-06 Corning Incorporated Cell culture insert
EP3234109A4 (en) 2014-12-19 2018-06-27 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
JP7420334B2 (ja) * 2015-06-25 2024-01-23 株式会社カネカ 液体注入方法
WO2017007278A1 (ko) * 2015-07-09 2017-01-12 사회복지법인 삼성생명공익재단 자동 세포 배양기 및 그 배양기의 동작 방법
EP3344755A4 (en) 2015-08-31 2019-05-22 I Peace, Inc. PREPARATION SYSTEM FOR PLURIPOTENTE STEM CELLS AND METHOD FOR THE PRODUCTION OF INDUCED PLURIPOTENTAL STEM CELLS
IL299626A (en) 2015-10-01 2023-03-01 Berkeley Lights Inc Multi-well plate incubator
CN117737124A (zh) 2015-10-16 2024-03-22 菲特治疗公司 用于诱导和维护基态多能性的平台
US11674952B2 (en) 2016-02-24 2023-06-13 The Rockefeller University Embryonic cell-based therapeutic candidate screening systems, models for Huntington's Disease and uses thereof
CN108779424A (zh) * 2016-03-21 2018-11-09 通用电气公司 使用搅拌槽生物反应器的多能干细胞扩增和传代
US10420803B2 (en) 2016-04-14 2019-09-24 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
JP2018000130A (ja) * 2016-07-05 2018-01-11 株式会社Ihi 細胞培養装置
KR102536879B1 (ko) * 2016-07-19 2023-05-26 아셀라타 리미티드 현탁 내 만능줄기세포를 배양하기 위한 배양 배지
DE102016114043B3 (de) 2016-07-29 2017-08-10 Technische Universität Dresden Vorrichtung zur Isolierung von Stammzellen aus fötalen Geweben
CN115044471B (zh) 2016-08-27 2025-05-27 三维生物科技有限公司 生物反应器
CN109923588B (zh) 2016-11-10 2022-12-02 伯克顿迪金森公司 用于监测培养基方案的时间线系统
EP3548602B1 (en) 2016-12-01 2024-09-25 Bruker Cellular Analysis, Inc. Well-plate incubator
CA3053891C (en) * 2017-02-27 2023-05-23 I Peace, Inc. Somatic cell production system
CA3054272C (en) * 2017-02-27 2023-01-24 I Peace, Inc. Cell processing system and cell processing apparatus
JP7197557B2 (ja) 2017-07-14 2022-12-27 コーニング インコーポレイテッド 多孔性支持体を有する細胞培養槽
PL3652292T3 (pl) 2017-07-14 2025-09-01 Corning Incorporated Naczynie do hodowli komórkowej 3d i sposoby hodowli komórkowej 3d
US11857970B2 (en) 2017-07-14 2024-01-02 Corning Incorporated Cell culture vessel
JP7245222B2 (ja) 2017-07-14 2023-03-23 コーニング インコーポレイテッド 手動又は自動で培地を交換するための３ｄ細胞培養容器
KR102220125B1 (ko) * 2018-04-26 2021-02-25 (주)세포바이오 세포배양 자동화 장치 및 방법
WO2020013845A1 (en) 2018-07-13 2020-01-16 Corning Incorporated Cell culture vessels with stabilizer devices
CN111065725B (zh) 2018-07-13 2024-03-29 康宁股份有限公司 包括具有互联的壁的微板的流体装置
JP7171695B2 (ja) 2018-07-13 2022-11-15 コーニング インコーポレイテッド 液体培地送達面を含む側壁を有するマイクロキャビティ皿
US20230035127A1 (en) * 2021-07-21 2023-02-02 University Of Maryland, College Park Culture and differentiation of pluripotent stem cells

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352883A (en) 1979-03-28 1982-10-05 Damon Corporation Encapsulation of biological material
US4284412A (en) 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
JPS58155087A (ja) * 1982-03-12 1983-09-14 Olympus Optical Co Ltd 細胞の自動培養装置
US4498766A (en) 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4857451A (en) 1984-12-24 1989-08-15 Flow Cytometry Standards Corporation Method of compensating and calibrating a flow cytometer, and microbead standards kit therefor
US4774189A (en) 1984-12-24 1988-09-27 Flow Cytometry Standards Corp. Fluorescent calibration microbeads simulating stained cells
US4767206A (en) 1984-12-24 1988-08-30 Flow Cytometry Standards Corporation Calibration method for flow cytometry using fluorescent microbeads and synthesis thereof
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
US4714682A (en) 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US5160974A (en) 1990-06-25 1992-11-03 Flow Science, Inc. Closed sample cell for use in flow cytometry
US5478722A (en) 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
WO1994017178A1 (en) 1993-01-29 1994-08-04 New Brunswick Scientific Co., Inc. Method and apparatus for anchorage and suspension cell culture
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6325114B1 (en) 2000-02-01 2001-12-04 Incyte Genomics, Inc. Pipetting station apparatus
WO2003087292A2 (en) * 2002-04-08 2003-10-23 Millenium Biologix Inc. Automated tissue engineering system
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
ATE549395T1 (de) * 2004-09-08 2012-03-15 Wisconsin Alumni Res Found Medium und kultur von embryonalen stammzellen
US20060198827A1 (en) 2005-02-04 2006-09-07 Shulamit Levenberg Engineering vascularized muscle tissue
US20060199265A1 (en) 2005-03-02 2006-09-07 Wolf Michael F Seeding implantable medical devices with cells

Also Published As

Publication number Publication date
JP2014110817A (ja) 2014-06-19
US20130316445A1 (en) 2013-11-28
US8815585B2 (en) 2014-08-26
AU2008272949B2 (en) 2014-05-15
EP2173863B1 (en) 2018-10-10
DK2173863T3 (en) 2019-01-21
AU2008272949A1 (en) 2009-01-08
JP2017023158A (ja) 2017-02-02
JP5991796B2 (ja) 2016-09-14
WO2009006422A1 (en) 2009-01-08
CA2691793A1 (en) 2009-01-08
DK3293256T3 (da) 2019-08-12
US20090029462A1 (en) 2009-01-29
JP2010532173A (ja) 2010-10-07
EP2173863A1 (en) 2010-04-14
KR20100059789A (ko) 2010-06-04
EP3293256A1 (en) 2018-03-14
JP2016168056A (ja) 2016-09-23
EP3293256B1 (en) 2019-05-22

Similar Documents

Publication Publication Date Title
KR20160005142A (ko) 배아줄기세포 배양을 위한 자동화 방법 및 장치
JP7510247B2 (ja) 細胞凝集塊を解離させる方法
CN107267442A (zh) 微载体上的多能干细胞培养
JP2026062859A (ja) バイオリアクターにおける懸濁状態での幹細胞の増殖方法
JP2025179174A (ja) ヒト多能性幹細胞製造のためのエンドツーエンドプラットフォーム
CN119855898A (zh) iPSC在生物反应器中的分化
AU2014201955B2 (en) Automated method and apparatus for embryonic stem cell culture
Elseberg et al. The challenge of human mesenchymal stromal cell expansion: current and prospective answers
US20250122474A1 (en) Production method for natural killer cells
US20190359946A1 (en) Passaging and harvesting formulation for single-cell human pluripotent stem cells
EP4114919B1 (en) Automated method for preparing keratinocytes
Jing et al. Stem cell bioprocessing for regenerative medicine
WO2025062293A1 (en) Methods and materials for scalable 3d cellular redifferentiation
WO2025146565A1 (en) Scalable generation of human induced pluripotent stem cell derived cardiac organoids
JP2025541330A (ja) 細胞の再分化及び増殖のための材料、方法及びシステム
HK40119737A (en) Production method for natural killer cells
Tristan et al. Vukasin M. Jovanovic, Yeliz Gedik, Yogita Jethmalani, Charles Bonney, Elena Barnaeva, John Braisted, Sunil K. Mallanna, Dorjbal Dorjsuren, Michael J. Iannotti, Ty C. Voss, Sam Michael, Anton Simeonov, and Ilyas Singeç

Legal Events

Date Code Title Description
A107 Divisional application of patent
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20151228

Application number text: 1020107002003

Filing date: 20100128

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20160127

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20170816

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20171101

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20170816

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I