JP5882058B2 - 組合せ抗体ライブラリー及びその使用 - Google Patents
組合せ抗体ライブラリー及びその使用 Download PDFInfo
- Publication number
- JP5882058B2 JP5882058B2 JP2011535649A JP2011535649A JP5882058B2 JP 5882058 B2 JP5882058 B2 JP 5882058B2 JP 2011535649 A JP2011535649 A JP 2011535649A JP 2011535649 A JP2011535649 A JP 2011535649A JP 5882058 B2 JP5882058 B2 JP 5882058B2
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- germline
- chain
- human
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000004602 germ cell Anatomy 0.000 claims description 561
- 150000007523 nucleic acids Chemical class 0.000 claims description 540
- 102000039446 nucleic acids Human genes 0.000 claims description 408
- 108020004707 nucleic acids Proteins 0.000 claims description 408
- 108090000623 proteins and genes Proteins 0.000 claims description 351
- 238000000034 method Methods 0.000 claims description 268
- 241000282414 Homo sapiens Species 0.000 claims description 192
- 230000027455 binding Effects 0.000 claims description 154
- 238000009739 binding Methods 0.000 claims description 152
- 102000004169 proteins and genes Human genes 0.000 claims description 127
- 150000001413 amino acids Chemical group 0.000 claims description 124
- 239000000427 antigen Substances 0.000 claims description 117
- 102000036639 antigens Human genes 0.000 claims description 117
- 108091007433 antigens Proteins 0.000 claims description 117
- 108020004705 Codon Proteins 0.000 claims description 79
- 230000000694 effects Effects 0.000 claims description 66
- -1 IGHV2- 5 Proteins 0.000 claims description 57
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims description 56
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims description 56
- 239000012634 fragment Substances 0.000 claims description 51
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims description 46
- 239000000203 mixture Substances 0.000 claims description 44
- 108700028369 Alleles Proteins 0.000 claims description 43
- 230000037431 insertion Effects 0.000 claims description 36
- 238000003780 insertion Methods 0.000 claims description 36
- 238000006467 substitution reaction Methods 0.000 claims description 32
- 230000005714 functional activity Effects 0.000 claims description 30
- 238000012216 screening Methods 0.000 claims description 26
- 230000015572 biosynthetic process Effects 0.000 claims description 20
- 101001079285 Homo sapiens Immunoglobulin heavy joining 1 Proteins 0.000 claims description 19
- 102100028078 Immunoglobulin heavy joining 1 Human genes 0.000 claims description 19
- 101000998947 Homo sapiens Immunoglobulin heavy variable 1-46 Proteins 0.000 claims description 18
- 101001091242 Homo sapiens Immunoglobulin kappa joining 1 Proteins 0.000 claims description 18
- 102100036888 Immunoglobulin heavy variable 1-46 Human genes 0.000 claims description 18
- 102100034892 Immunoglobulin kappa joining 1 Human genes 0.000 claims description 18
- 239000000816 peptidomimetic Substances 0.000 claims description 18
- 102000005962 receptors Human genes 0.000 claims description 17
- 108020003175 receptors Proteins 0.000 claims description 17
- 239000007787 solid Substances 0.000 claims description 15
- 241000894007 species Species 0.000 claims description 12
- 101001134437 Homo sapiens Immunoglobulin lambda joining 1 Proteins 0.000 claims description 11
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims description 11
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims description 11
- 102100034172 Immunoglobulin lambda joining 1 Human genes 0.000 claims description 11
- 102100023752 Immunoglobulin lambda variable 8-61 Human genes 0.000 claims description 11
- 238000000126 in silico method Methods 0.000 claims description 10
- 239000003446 ligand Substances 0.000 claims description 10
- 101000978126 Homo sapiens Immunoglobulin lambda variable 8-61 Proteins 0.000 claims description 9
- 102100029416 Immunoglobulin heavy variable 6-1 Human genes 0.000 claims description 9
- 102000000844 Cell Surface Receptors Human genes 0.000 claims description 8
- 108010001857 Cell Surface Receptors Proteins 0.000 claims description 8
- 102100036509 Erythropoietin receptor Human genes 0.000 claims description 8
- 101000998950 Homo sapiens Immunoglobulin heavy variable 1-18 Proteins 0.000 claims description 8
- 101001138128 Homo sapiens Immunoglobulin kappa variable 1-12 Proteins 0.000 claims description 8
- 101000604674 Homo sapiens Immunoglobulin kappa variable 4-1 Proteins 0.000 claims description 8
- 102100036884 Immunoglobulin heavy variable 1-18 Human genes 0.000 claims description 8
- 102100020773 Immunoglobulin kappa variable 1-12 Human genes 0.000 claims description 8
- 102100038198 Immunoglobulin kappa variable 4-1 Human genes 0.000 claims description 8
- 102100033553 Delta-like protein 4 Human genes 0.000 claims description 7
- 101000989060 Homo sapiens Immunoglobulin heavy variable 6-1 Proteins 0.000 claims description 7
- 101001047629 Homo sapiens Immunoglobulin kappa variable 2-30 Proteins 0.000 claims description 7
- 102100022952 Immunoglobulin kappa variable 2-30 Human genes 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 102000001301 EGF receptor Human genes 0.000 claims description 6
- 108060006698 EGF receptor Proteins 0.000 claims description 6
- 101000843762 Homo sapiens Immunoglobulin heavy diversity 1-1 Proteins 0.000 claims description 6
- 101001037142 Homo sapiens Immunoglobulin heavy variable 3-20 Proteins 0.000 claims description 6
- 101001138089 Homo sapiens Immunoglobulin kappa variable 1-39 Proteins 0.000 claims description 6
- 102100030644 Immunoglobulin heavy diversity 1-1 Human genes 0.000 claims description 6
- 102100040218 Immunoglobulin heavy variable 3-20 Human genes 0.000 claims description 6
- 102100029414 Immunoglobulin heavy variable 5-51 Human genes 0.000 claims description 6
- 102100020910 Immunoglobulin kappa variable 1-39 Human genes 0.000 claims description 6
- 101000998953 Homo sapiens Immunoglobulin heavy variable 1-2 Proteins 0.000 claims description 5
- 101000998952 Homo sapiens Immunoglobulin heavy variable 1-3 Proteins 0.000 claims description 5
- 101000998948 Homo sapiens Immunoglobulin heavy variable 1-45 Proteins 0.000 claims description 5
- 101001002931 Homo sapiens Immunoglobulin heavy variable 1-58 Proteins 0.000 claims description 5
- 101001037147 Homo sapiens Immunoglobulin heavy variable 1-69 Proteins 0.000 claims description 5
- 101000998951 Homo sapiens Immunoglobulin heavy variable 1-8 Proteins 0.000 claims description 5
- 101001037138 Homo sapiens Immunoglobulin heavy variable 3-11 Proteins 0.000 claims description 5
- 101000839665 Homo sapiens Immunoglobulin heavy variable 3-43 Proteins 0.000 claims description 5
- 101000839663 Homo sapiens Immunoglobulin heavy variable 3-49 Proteins 0.000 claims description 5
- 101000839660 Homo sapiens Immunoglobulin heavy variable 3-53 Proteins 0.000 claims description 5
- 101000839658 Homo sapiens Immunoglobulin heavy variable 3-66 Proteins 0.000 claims description 5
- 101001037153 Homo sapiens Immunoglobulin heavy variable 3-7 Proteins 0.000 claims description 5
- 101000839659 Homo sapiens Immunoglobulin heavy variable 3-72 Proteins 0.000 claims description 5
- 101000839657 Homo sapiens Immunoglobulin heavy variable 3-73 Proteins 0.000 claims description 5
- 101000839687 Homo sapiens Immunoglobulin heavy variable 3-74 Proteins 0.000 claims description 5
- 101001037144 Homo sapiens Immunoglobulin heavy variable 3-9 Proteins 0.000 claims description 5
- 101000839664 Homo sapiens Probable non-functional immunoglobulin heavy variable 3-38 Proteins 0.000 claims description 5
- 102100036887 Immunoglobulin heavy variable 1-2 Human genes 0.000 claims description 5
- 102100036886 Immunoglobulin heavy variable 1-3 Human genes 0.000 claims description 5
- 102100036889 Immunoglobulin heavy variable 1-45 Human genes 0.000 claims description 5
- 102100020774 Immunoglobulin heavy variable 1-58 Human genes 0.000 claims description 5
- 102100040232 Immunoglobulin heavy variable 1-69 Human genes 0.000 claims description 5
- 102100036885 Immunoglobulin heavy variable 1-8 Human genes 0.000 claims description 5
- 102100040222 Immunoglobulin heavy variable 3-11 Human genes 0.000 claims description 5
- 102100028315 Immunoglobulin heavy variable 3-43 Human genes 0.000 claims description 5
- 102100028319 Immunoglobulin heavy variable 3-49 Human genes 0.000 claims description 5
- 102100028317 Immunoglobulin heavy variable 3-53 Human genes 0.000 claims description 5
- 102100027821 Immunoglobulin heavy variable 3-66 Human genes 0.000 claims description 5
- 102100040231 Immunoglobulin heavy variable 3-7 Human genes 0.000 claims description 5
- 102100027820 Immunoglobulin heavy variable 3-72 Human genes 0.000 claims description 5
- 102100027822 Immunoglobulin heavy variable 3-73 Human genes 0.000 claims description 5
- 102100028305 Immunoglobulin heavy variable 3-74 Human genes 0.000 claims description 5
- 102100040234 Immunoglobulin heavy variable 3-9 Human genes 0.000 claims description 5
- 102100028306 Immunoglobulin heavy variable 4-34 Human genes 0.000 claims description 5
- 102100028312 Immunoglobulin heavy variable 4-39 Human genes 0.000 claims description 5
- 102100028405 Immunoglobulin heavy variable 4-59 Human genes 0.000 claims description 5
- 102100029419 Immunoglobulin heavy variable 4-61 Human genes 0.000 claims description 5
- 206010028980 Neoplasm Diseases 0.000 claims description 5
- 108010070047 Notch Receptors Proteins 0.000 claims description 5
- 102100028318 Probable non-functional immunoglobulin heavy variable 3-38 Human genes 0.000 claims description 5
- 201000011510 cancer Diseases 0.000 claims description 5
- 230000019491 signal transduction Effects 0.000 claims description 5
- 102000000905 Cadherin Human genes 0.000 claims description 4
- 108050007957 Cadherin Proteins 0.000 claims description 4
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 claims description 4
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 claims description 4
- 101500026735 Homo sapiens Brain natriuretic peptide 32 Proteins 0.000 claims description 4
- 101000998949 Homo sapiens Immunoglobulin heavy variable 1-24 Proteins 0.000 claims description 4
- 101001037151 Homo sapiens Immunoglobulin heavy variable 2-70 Proteins 0.000 claims description 4
- 101001037137 Homo sapiens Immunoglobulin heavy variable 3-13 Proteins 0.000 claims description 4
- 101001037136 Homo sapiens Immunoglobulin heavy variable 3-15 Proteins 0.000 claims description 4
- 101001037141 Homo sapiens Immunoglobulin heavy variable 3-21 Proteins 0.000 claims description 4
- 101001037139 Homo sapiens Immunoglobulin heavy variable 3-30 Proteins 0.000 claims description 4
- 101001037143 Homo sapiens Immunoglobulin heavy variable 3-33 Proteins 0.000 claims description 4
- 101000839662 Homo sapiens Immunoglobulin heavy variable 3-48 Proteins 0.000 claims description 4
- 101000839661 Homo sapiens Immunoglobulin heavy variable 3-64 Proteins 0.000 claims description 4
- 101000839683 Homo sapiens Immunoglobulin heavy variable 4-28 Proteins 0.000 claims description 4
- 101000989062 Homo sapiens Immunoglobulin heavy variable 5-51 Proteins 0.000 claims description 4
- 101001138126 Homo sapiens Immunoglobulin kappa variable 1-16 Proteins 0.000 claims description 4
- 101001138125 Homo sapiens Immunoglobulin kappa variable 1-17 Proteins 0.000 claims description 4
- 101001138123 Homo sapiens Immunoglobulin kappa variable 1-27 Proteins 0.000 claims description 4
- 101001138121 Homo sapiens Immunoglobulin kappa variable 1-33 Proteins 0.000 claims description 4
- 101001138133 Homo sapiens Immunoglobulin kappa variable 1-5 Proteins 0.000 claims description 4
- 101001138130 Homo sapiens Immunoglobulin kappa variable 1-9 Proteins 0.000 claims description 4
- 101001008259 Homo sapiens Immunoglobulin kappa variable 1D-12 Proteins 0.000 claims description 4
- 101001008261 Homo sapiens Immunoglobulin kappa variable 1D-13 Proteins 0.000 claims description 4
- 101001008333 Homo sapiens Immunoglobulin kappa variable 1D-16 Proteins 0.000 claims description 4
- 101001008329 Homo sapiens Immunoglobulin kappa variable 1D-33 Proteins 0.000 claims description 4
- 101001008313 Homo sapiens Immunoglobulin kappa variable 1D-39 Proteins 0.000 claims description 4
- 101001008255 Homo sapiens Immunoglobulin kappa variable 1D-8 Proteins 0.000 claims description 4
- 101001047626 Homo sapiens Immunoglobulin kappa variable 2-24 Proteins 0.000 claims description 4
- 101001047627 Homo sapiens Immunoglobulin kappa variable 2-28 Proteins 0.000 claims description 4
- 101001047628 Homo sapiens Immunoglobulin kappa variable 2-29 Proteins 0.000 claims description 4
- 101001047625 Homo sapiens Immunoglobulin kappa variable 2-40 Proteins 0.000 claims description 4
- 101001008321 Homo sapiens Immunoglobulin kappa variable 2D-26 Proteins 0.000 claims description 4
- 101001008293 Homo sapiens Immunoglobulin kappa variable 2D-40 Proteins 0.000 claims description 4
- 101001047618 Homo sapiens Immunoglobulin kappa variable 3-15 Proteins 0.000 claims description 4
- 101001008263 Homo sapiens Immunoglobulin kappa variable 3D-15 Proteins 0.000 claims description 4
- 101001008315 Homo sapiens Immunoglobulin kappa variable 3D-20 Proteins 0.000 claims description 4
- 101000605181 Homo sapiens Immunoglobulin kappa variable 5-2 Proteins 0.000 claims description 4
- 101001090250 Homo sapiens Immunoglobulin kappa variable 6-21 Proteins 0.000 claims description 4
- 101001008317 Homo sapiens Immunoglobulin kappa variable 6D-21 Proteins 0.000 claims description 4
- 101001054844 Homo sapiens Immunoglobulin lambda variable 1-36 Proteins 0.000 claims description 4
- 101001054843 Homo sapiens Immunoglobulin lambda variable 1-40 Proteins 0.000 claims description 4
- 101001054838 Homo sapiens Immunoglobulin lambda variable 1-44 Proteins 0.000 claims description 4
- 101001054837 Homo sapiens Immunoglobulin lambda variable 1-47 Proteins 0.000 claims description 4
- 101001054839 Homo sapiens Immunoglobulin lambda variable 1-51 Proteins 0.000 claims description 4
- 101000578462 Homo sapiens Immunoglobulin lambda variable 10-54 Proteins 0.000 claims description 4
- 101000956884 Homo sapiens Immunoglobulin lambda variable 2-11 Proteins 0.000 claims description 4
- 101000956888 Homo sapiens Immunoglobulin lambda variable 2-18 Proteins 0.000 claims description 4
- 101000956889 Homo sapiens Immunoglobulin lambda variable 2-23 Proteins 0.000 claims description 4
- 101000956887 Homo sapiens Immunoglobulin lambda variable 2-8 Proteins 0.000 claims description 4
- 101001005360 Homo sapiens Immunoglobulin lambda variable 3-1 Proteins 0.000 claims description 4
- 101001005361 Homo sapiens Immunoglobulin lambda variable 3-10 Proteins 0.000 claims description 4
- 101001005363 Homo sapiens Immunoglobulin lambda variable 3-16 Proteins 0.000 claims description 4
- 101001005364 Homo sapiens Immunoglobulin lambda variable 3-19 Proteins 0.000 claims description 4
- 101001005365 Homo sapiens Immunoglobulin lambda variable 3-21 Proteins 0.000 claims description 4
- 101001005336 Homo sapiens Immunoglobulin lambda variable 3-25 Proteins 0.000 claims description 4
- 101001005337 Homo sapiens Immunoglobulin lambda variable 3-27 Proteins 0.000 claims description 4
- 101001005329 Homo sapiens Immunoglobulin lambda variable 3-9 Proteins 0.000 claims description 4
- 101001005330 Homo sapiens Immunoglobulin lambda variable 4-3 Proteins 0.000 claims description 4
- 101001005331 Homo sapiens Immunoglobulin lambda variable 4-60 Proteins 0.000 claims description 4
- 101001005332 Homo sapiens Immunoglobulin lambda variable 4-69 Proteins 0.000 claims description 4
- 101001005333 Homo sapiens Immunoglobulin lambda variable 5-37 Proteins 0.000 claims description 4
- 101001005334 Homo sapiens Immunoglobulin lambda variable 5-39 Proteins 0.000 claims description 4
- 101001005335 Homo sapiens Immunoglobulin lambda variable 5-45 Proteins 0.000 claims description 4
- 101000978130 Homo sapiens Immunoglobulin lambda variable 5-52 Proteins 0.000 claims description 4
- 101000978127 Homo sapiens Immunoglobulin lambda variable 7-46 Proteins 0.000 claims description 4
- 101001037135 Homo sapiens Probable non-functional immunoglobulin heavy variable 3-16 Proteins 0.000 claims description 4
- 101001008319 Homo sapiens Probable non-functional immunoglobulin kappa variable 2D-24 Proteins 0.000 claims description 4
- 101001047520 Homo sapiens Probable non-functional immunoglobulin kappa variable 3-7 Proteins 0.000 claims description 4
- 101001009875 Homo sapiens Probable non-functional immunoglobulin kappa variable 6D-41 Proteins 0.000 claims description 4
- 101000978120 Homo sapiens Probable non-functional immunoglobulin lambda variable 11-55 Proteins 0.000 claims description 4
- 101000956613 Homo sapiens Probable non-functional immunoglobulin lambda variable 2-33 Proteins 0.000 claims description 4
- 101001005338 Homo sapiens Probable non-functional immunoglobulin lambda variable 3-32 Proteins 0.000 claims description 4
- 101001138120 Homo sapiens Probable non-functional immunoglobulinn kappa variable 1-37 Proteins 0.000 claims description 4
- 101001008331 Homo sapiens Probable non-functional immunoglobulinn kappa variable 1D-37 Proteins 0.000 claims description 4
- 102100036890 Immunoglobulin heavy variable 1-24 Human genes 0.000 claims description 4
- 102100040233 Immunoglobulin heavy variable 2-70 Human genes 0.000 claims description 4
- 102100040221 Immunoglobulin heavy variable 3-13 Human genes 0.000 claims description 4
- 102100040224 Immunoglobulin heavy variable 3-15 Human genes 0.000 claims description 4
- 102100040217 Immunoglobulin heavy variable 3-21 Human genes 0.000 claims description 4
- 102100040219 Immunoglobulin heavy variable 3-30 Human genes 0.000 claims description 4
- 102100040236 Immunoglobulin heavy variable 3-33 Human genes 0.000 claims description 4
- 102100028320 Immunoglobulin heavy variable 3-48 Human genes 0.000 claims description 4
- 102100028321 Immunoglobulin heavy variable 3-64 Human genes 0.000 claims description 4
- 102100028311 Immunoglobulin heavy variable 4-28 Human genes 0.000 claims description 4
- 102100028308 Immunoglobulin heavy variable 4-4 Human genes 0.000 claims description 4
- 102100020946 Immunoglobulin kappa variable 1-16 Human genes 0.000 claims description 4
- 102100020945 Immunoglobulin kappa variable 1-17 Human genes 0.000 claims description 4
- 102100020902 Immunoglobulin kappa variable 1-27 Human genes 0.000 claims description 4
- 102100020901 Immunoglobulin kappa variable 1-33 Human genes 0.000 claims description 4
- 102100020769 Immunoglobulin kappa variable 1-5 Human genes 0.000 claims description 4
- 102100020770 Immunoglobulin kappa variable 1-9 Human genes 0.000 claims description 4
- 102100027412 Immunoglobulin kappa variable 1D-12 Human genes 0.000 claims description 4
- 102100027411 Immunoglobulin kappa variable 1D-13 Human genes 0.000 claims description 4
- 102100027462 Immunoglobulin kappa variable 1D-16 Human genes 0.000 claims description 4
- 102100027464 Immunoglobulin kappa variable 1D-33 Human genes 0.000 claims description 4
- 102100027404 Immunoglobulin kappa variable 1D-39 Human genes 0.000 claims description 4
- 102100027406 Immunoglobulin kappa variable 1D-8 Human genes 0.000 claims description 4
- 102100022947 Immunoglobulin kappa variable 2-24 Human genes 0.000 claims description 4
- 102100022950 Immunoglobulin kappa variable 2-28 Human genes 0.000 claims description 4
- 102100022949 Immunoglobulin kappa variable 2-29 Human genes 0.000 claims description 4
- 102100022948 Immunoglobulin kappa variable 2-40 Human genes 0.000 claims description 4
- 102100027460 Immunoglobulin kappa variable 2D-26 Human genes 0.000 claims description 4
- 102100027409 Immunoglobulin kappa variable 2D-40 Human genes 0.000 claims description 4
- 102100022965 Immunoglobulin kappa variable 3-15 Human genes 0.000 claims description 4
- 102100027410 Immunoglobulin kappa variable 3D-15 Human genes 0.000 claims description 4
- 102100027403 Immunoglobulin kappa variable 3D-20 Human genes 0.000 claims description 4
- 102100038241 Immunoglobulin kappa variable 5-2 Human genes 0.000 claims description 4
- 102100034806 Immunoglobulin kappa variable 6-21 Human genes 0.000 claims description 4
- 102100027402 Immunoglobulin kappa variable 6D-21 Human genes 0.000 claims description 4
- 102100026916 Immunoglobulin lambda variable 1-36 Human genes 0.000 claims description 4
- 102100026911 Immunoglobulin lambda variable 1-40 Human genes 0.000 claims description 4
- 102100026921 Immunoglobulin lambda variable 1-44 Human genes 0.000 claims description 4
- 102100026809 Immunoglobulin lambda variable 1-47 Human genes 0.000 claims description 4
- 102100026922 Immunoglobulin lambda variable 1-51 Human genes 0.000 claims description 4
- 102100027972 Immunoglobulin lambda variable 10-54 Human genes 0.000 claims description 4
- 102100038432 Immunoglobulin lambda variable 2-11 Human genes 0.000 claims description 4
- 102100038431 Immunoglobulin lambda variable 2-18 Human genes 0.000 claims description 4
- 102100038430 Immunoglobulin lambda variable 2-23 Human genes 0.000 claims description 4
- 102100038428 Immunoglobulin lambda variable 2-8 Human genes 0.000 claims description 4
- 102100025921 Immunoglobulin lambda variable 3-1 Human genes 0.000 claims description 4
- 102100025919 Immunoglobulin lambda variable 3-10 Human genes 0.000 claims description 4
- 102100025936 Immunoglobulin lambda variable 3-16 Human genes 0.000 claims description 4
- 102100025937 Immunoglobulin lambda variable 3-19 Human genes 0.000 claims description 4
- 102100025934 Immunoglobulin lambda variable 3-21 Human genes 0.000 claims description 4
- 102100025876 Immunoglobulin lambda variable 3-25 Human genes 0.000 claims description 4
- 102100025874 Immunoglobulin lambda variable 3-27 Human genes 0.000 claims description 4
- 102100025864 Immunoglobulin lambda variable 3-9 Human genes 0.000 claims description 4
- 102100025865 Immunoglobulin lambda variable 4-3 Human genes 0.000 claims description 4
- 102100025862 Immunoglobulin lambda variable 4-60 Human genes 0.000 claims description 4
- 102100025863 Immunoglobulin lambda variable 4-69 Human genes 0.000 claims description 4
- 102100025856 Immunoglobulin lambda variable 5-37 Human genes 0.000 claims description 4
- 102100025857 Immunoglobulin lambda variable 5-39 Human genes 0.000 claims description 4
- 102100025855 Immunoglobulin lambda variable 5-45 Human genes 0.000 claims description 4
- 102100023753 Immunoglobulin lambda variable 5-52 Human genes 0.000 claims description 4
- 102100023751 Immunoglobulin lambda variable 7-46 Human genes 0.000 claims description 4
- 108090000542 Lymphotoxin-alpha Proteins 0.000 claims description 4
- 102000004083 Lymphotoxin-alpha Human genes 0.000 claims description 4
- 102100040223 Probable non-functional immunoglobulin heavy variable 3-16 Human genes 0.000 claims description 4
- 102100027461 Probable non-functional immunoglobulin kappa variable 2D-24 Human genes 0.000 claims description 4
- 102100022959 Probable non-functional immunoglobulin kappa variable 3-7 Human genes 0.000 claims description 4
- 102100030885 Probable non-functional immunoglobulin kappa variable 6D-41 Human genes 0.000 claims description 4
- 102100023451 Probable non-functional immunoglobulin lambda variable 11-55 Human genes 0.000 claims description 4
- 102100038489 Probable non-functional immunoglobulin lambda variable 2-33 Human genes 0.000 claims description 4
- 102100025868 Probable non-functional immunoglobulin lambda variable 3-32 Human genes 0.000 claims description 4
- 102100020904 Probable non-functional immunoglobulinn kappa variable 1-37 Human genes 0.000 claims description 4
- 102100027463 Probable non-functional immunoglobulinn kappa variable 1D-37 Human genes 0.000 claims description 4
- 102100040247 Tumor necrosis factor Human genes 0.000 claims description 4
- 230000035755 proliferation Effects 0.000 claims description 4
- 102100032912 CD44 antigen Human genes 0.000 claims description 3
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 claims description 3
- 101001037152 Homo sapiens Immunoglobulin heavy variable 2-26 Proteins 0.000 claims description 3
- 101000839682 Homo sapiens Immunoglobulin heavy variable 4-34 Proteins 0.000 claims description 3
- 101000839679 Homo sapiens Immunoglobulin heavy variable 4-39 Proteins 0.000 claims description 3
- 101000839781 Homo sapiens Immunoglobulin heavy variable 4-59 Proteins 0.000 claims description 3
- 101000989076 Homo sapiens Immunoglobulin heavy variable 4-61 Proteins 0.000 claims description 3
- 101001008323 Homo sapiens Immunoglobulin kappa variable 2D-28 Proteins 0.000 claims description 3
- 101001008325 Homo sapiens Immunoglobulin kappa variable 2D-29 Proteins 0.000 claims description 3
- 101001005362 Homo sapiens Immunoglobulin lambda variable 3-12 Proteins 0.000 claims description 3
- 101001037134 Homo sapiens Probable non-functional immunoglobulin heavy variable 3-35 Proteins 0.000 claims description 3
- 101001009876 Homo sapiens Probable non-functional immunoglobulin kappa variable 1D-42 Proteins 0.000 claims description 3
- 101001054840 Homo sapiens Probable non-functional immunoglobulin lambda variable 1-50 Proteins 0.000 claims description 3
- 101000611183 Homo sapiens Tumor necrosis factor Proteins 0.000 claims description 3
- 102100040230 Immunoglobulin heavy variable 2-26 Human genes 0.000 claims description 3
- 102100027459 Immunoglobulin kappa variable 2D-28 Human genes 0.000 claims description 3
- 102100027458 Immunoglobulin kappa variable 2D-29 Human genes 0.000 claims description 3
- 102100025920 Immunoglobulin lambda variable 3-12 Human genes 0.000 claims description 3
- 102100040226 Probable non-functional immunoglobulin heavy variable 3-35 Human genes 0.000 claims description 3
- 102100030884 Probable non-functional immunoglobulin kappa variable 1D-42 Human genes 0.000 claims description 3
- 102100026912 Probable non-functional immunoglobulin lambda variable 1-50 Human genes 0.000 claims description 3
- 102000009524 Vascular Endothelial Growth Factor A Human genes 0.000 claims description 3
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 claims description 3
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims description 2
- BJHCYTJNPVGSBZ-YXSASFKJSA-N 1-[4-[6-amino-5-[(Z)-methoxyiminomethyl]pyrimidin-4-yl]oxy-2-chlorophenyl]-3-ethylurea Chemical compound CCNC(=O)Nc1ccc(Oc2ncnc(N)c2\C=N/OC)cc1Cl BJHCYTJNPVGSBZ-YXSASFKJSA-N 0.000 claims description 2
- 102100029822 B- and T-lymphocyte attenuator Human genes 0.000 claims description 2
- 102100022005 B-lymphocyte antigen CD20 Human genes 0.000 claims description 2
- 102100040843 C-type lectin domain family 4 member M Human genes 0.000 claims description 2
- 102100024217 CAMPATH-1 antigen Human genes 0.000 claims description 2
- 102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 claims description 2
- 108050005493 CD3 protein, epsilon/gamma/delta subunit Proteins 0.000 claims description 2
- 108010065524 CD52 Antigen Proteins 0.000 claims description 2
- 108010021064 CTLA-4 Antigen Proteins 0.000 claims description 2
- 229940045513 CTLA4 antagonist Drugs 0.000 claims description 2
- 102000016289 Cell Adhesion Molecules Human genes 0.000 claims description 2
- 108010067225 Cell Adhesion Molecules Proteins 0.000 claims description 2
- 102100039498 Cytotoxic T-lymphocyte protein 4 Human genes 0.000 claims description 2
- 101710102442 Erythropoietin receptor Proteins 0.000 claims description 2
- 108010011459 Exenatide Proteins 0.000 claims description 2
- 108010008177 Fd immunoglobulins Proteins 0.000 claims description 2
- 101710198884 GATA-type zinc finger protein 1 Proteins 0.000 claims description 2
- 108060003199 Glucagon Proteins 0.000 claims description 2
- 102400000321 Glucagon Human genes 0.000 claims description 2
- DTHNMHAUYICORS-KTKZVXAJSA-N Glucagon-like peptide 1 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1N=CNC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 DTHNMHAUYICORS-KTKZVXAJSA-N 0.000 claims description 2
- 102400000326 Glucagon-like peptide 2 Human genes 0.000 claims description 2
- 101800000221 Glucagon-like peptide 2 Proteins 0.000 claims description 2
- 102100039622 Granulocyte colony-stimulating factor receptor Human genes 0.000 claims description 2
- 101710142125 Granulocyte colony-stimulating factor receptor Proteins 0.000 claims description 2
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 claims description 2
- 102100039620 Granulocyte-macrophage colony-stimulating factor Human genes 0.000 claims description 2
- 101000864344 Homo sapiens B- and T-lymphocyte attenuator Proteins 0.000 claims description 2
- 101000897405 Homo sapiens B-lymphocyte antigen CD20 Proteins 0.000 claims description 2
- 101000749311 Homo sapiens C-type lectin domain family 4 member M Proteins 0.000 claims description 2
- 101000839686 Homo sapiens Immunoglobulin heavy variable 4-4 Proteins 0.000 claims description 2
- 101001138132 Homo sapiens Immunoglobulin kappa variable 1-6 Proteins 0.000 claims description 2
- 101001005366 Homo sapiens Immunoglobulin lambda variable 3-22 Proteins 0.000 claims description 2
- 101000978133 Homo sapiens Immunoglobulin lambda variable 6-57 Proteins 0.000 claims description 2
- 101000978129 Homo sapiens Immunoglobulin lambda variable 9-49 Proteins 0.000 claims description 2
- 101001057504 Homo sapiens Interferon-stimulated gene 20 kDa protein Proteins 0.000 claims description 2
- 101001055144 Homo sapiens Interleukin-2 receptor subunit alpha Proteins 0.000 claims description 2
- 101001001487 Homo sapiens Phosphatidylinositol-glycan biosynthesis class F protein Proteins 0.000 claims description 2
- 101000595923 Homo sapiens Placenta growth factor Proteins 0.000 claims description 2
- 101000989063 Homo sapiens Probable non-functional immunoglobulin heavy variable 7-81 Proteins 0.000 claims description 2
- 102100020768 Immunoglobulin kappa variable 1-6 Human genes 0.000 claims description 2
- 102100025935 Immunoglobulin lambda variable 3-22 Human genes 0.000 claims description 2
- 102100023747 Immunoglobulin lambda variable 6-57 Human genes 0.000 claims description 2
- 102100023754 Immunoglobulin lambda variable 9-49 Human genes 0.000 claims description 2
- 102100026878 Interleukin-2 receptor subunit alpha Human genes 0.000 claims description 2
- 108010002616 Interleukin-5 Proteins 0.000 claims description 2
- 229940124761 MMP inhibitor Drugs 0.000 claims description 2
- 108010061593 Member 14 Tumor Necrosis Factor Receptors Proteins 0.000 claims description 2
- 108010004684 Pituitary adenylate cyclase-activating polypeptide Proteins 0.000 claims description 2
- 102100035194 Placenta growth factor Human genes 0.000 claims description 2
- 102100033237 Pro-epidermal growth factor Human genes 0.000 claims description 2
- 102100040918 Pro-glucagon Human genes 0.000 claims description 2
- 102100029413 Probable non-functional immunoglobulin heavy variable 7-81 Human genes 0.000 claims description 2
- 102000001253 Protein Kinase Human genes 0.000 claims description 2
- 108091005682 Receptor kinases Proteins 0.000 claims description 2
- 101710113649 Thyroid peroxidase Proteins 0.000 claims description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims description 2
- 102100028785 Tumor necrosis factor receptor superfamily member 14 Human genes 0.000 claims description 2
- 102100033732 Tumor necrosis factor receptor superfamily member 1A Human genes 0.000 claims description 2
- 101710187743 Tumor necrosis factor receptor superfamily member 1A Proteins 0.000 claims description 2
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 claims description 2
- 108010073923 Vascular Endothelial Growth Factor C Proteins 0.000 claims description 2
- 108010073919 Vascular Endothelial Growth Factor D Proteins 0.000 claims description 2
- 108010053096 Vascular Endothelial Growth Factor Receptor-1 Proteins 0.000 claims description 2
- 108010053099 Vascular Endothelial Growth Factor Receptor-2 Proteins 0.000 claims description 2
- 102100038232 Vascular endothelial growth factor C Human genes 0.000 claims description 2
- 102100038234 Vascular endothelial growth factor D Human genes 0.000 claims description 2
- 102100033178 Vascular endothelial growth factor receptor 1 Human genes 0.000 claims description 2
- 102100033177 Vascular endothelial growth factor receptor 2 Human genes 0.000 claims description 2
- 230000006907 apoptotic process Effects 0.000 claims description 2
- 230000004709 cell invasion Effects 0.000 claims description 2
- 230000004663 cell proliferation Effects 0.000 claims description 2
- JUFFVKRROAPVBI-PVOYSMBESA-N chembl1210015 Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(=O)N[C@H]1[C@@H]([C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@]3(O[C@@H](C[C@H](O)[C@H](O)CO)[C@H](NC(C)=O)[C@@H](O)C3)C(O)=O)O2)O)[C@@H](CO)O1)NC(C)=O)C(=O)NCC(=O)NCC(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CO)C(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C(C)C)C1=CC=CC=C1 JUFFVKRROAPVBI-PVOYSMBESA-N 0.000 claims description 2
- 230000035605 chemotaxis Effects 0.000 claims description 2
- 102000003675 cytokine receptors Human genes 0.000 claims description 2
- 108010057085 cytokine receptors Proteins 0.000 claims description 2
- 230000003511 endothelial effect Effects 0.000 claims description 2
- 229960001519 exenatide Drugs 0.000 claims description 2
- MASNOZXLGMXCHN-ZLPAWPGGSA-N glucagon Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 MASNOZXLGMXCHN-ZLPAWPGGSA-N 0.000 claims description 2
- 229960004666 glucagon Drugs 0.000 claims description 2
- TWSALRJGPBVBQU-PKQQPRCHSA-N glucagon-like peptide 2 Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(O)=O)[C@@H](C)CC)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)[C@@H](C)CC)C1=CC=CC=C1 TWSALRJGPBVBQU-PKQQPRCHSA-N 0.000 claims description 2
- 108010044426 integrins Proteins 0.000 claims description 2
- 102000006495 integrins Human genes 0.000 claims description 2
- 230000008611 intercellular interaction Effects 0.000 claims description 2
- 108010082117 matrigel Proteins 0.000 claims description 2
- 108010092408 Eosinophil Peroxidase Proteins 0.000 claims 2
- 101150029707 ERBB2 gene Proteins 0.000 claims 1
- 102100028471 Eosinophil peroxidase Human genes 0.000 claims 1
- 102100021866 Hepatocyte growth factor Human genes 0.000 claims 1
- 101000872077 Homo sapiens Delta-like protein 4 Proteins 0.000 claims 1
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims 1
- 101001037140 Homo sapiens Immunoglobulin heavy variable 3-23 Proteins 0.000 claims 1
- 101001047617 Homo sapiens Immunoglobulin kappa variable 3-11 Proteins 0.000 claims 1
- 101001047619 Homo sapiens Immunoglobulin kappa variable 3-20 Proteins 0.000 claims 1
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims 1
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims 1
- 102000009786 Immunoglobulin Constant Regions Human genes 0.000 claims 1
- 108010009817 Immunoglobulin Constant Regions Proteins 0.000 claims 1
- 102100040220 Immunoglobulin heavy variable 3-23 Human genes 0.000 claims 1
- 102100022955 Immunoglobulin kappa variable 3-11 Human genes 0.000 claims 1
- 102100022964 Immunoglobulin kappa variable 3-20 Human genes 0.000 claims 1
- 102000014736 Notch Human genes 0.000 claims 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims 1
- 102400000979 Pituitary adenylate cyclase-activating polypeptide 38 Human genes 0.000 claims 1
- 101710098940 Pro-epidermal growth factor Proteins 0.000 claims 1
- 101710100969 Receptor tyrosine-protein kinase erbB-3 Proteins 0.000 claims 1
- 102100033733 Tumor necrosis factor receptor superfamily member 1B Human genes 0.000 claims 1
- 101710187830 Tumor necrosis factor receptor superfamily member 1B Proteins 0.000 claims 1
- 108010073925 Vascular Endothelial Growth Factor B Proteins 0.000 claims 1
- 108010053100 Vascular Endothelial Growth Factor Receptor-3 Proteins 0.000 claims 1
- 102100038217 Vascular endothelial growth factor B Human genes 0.000 claims 1
- 102100033179 Vascular endothelial growth factor receptor 3 Human genes 0.000 claims 1
- UFTCZKMBJOPXDM-XXFCQBPRSA-N pituitary adenylate cyclase-activating polypeptide Chemical compound C([C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(N)=O)C1=CN=CN1 UFTCZKMBJOPXDM-XXFCQBPRSA-N 0.000 claims 1
- 230000002194 synthesizing effect Effects 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 description 250
- 239000002773 nucleotide Substances 0.000 description 245
- 108090000765 processed proteins & peptides Proteins 0.000 description 156
- 235000001014 amino acid Nutrition 0.000 description 121
- 229940024606 amino acid Drugs 0.000 description 115
- 102000004196 processed proteins & peptides Human genes 0.000 description 111
- 108091028043 Nucleic acid sequence Proteins 0.000 description 107
- 229920001184 polypeptide Polymers 0.000 description 88
- 239000013598 vector Substances 0.000 description 85
- 210000004027 cell Anatomy 0.000 description 67
- 235000018102 proteins Nutrition 0.000 description 61
- 230000014509 gene expression Effects 0.000 description 59
- 102000053602 DNA Human genes 0.000 description 47
- 108020004414 DNA Proteins 0.000 description 46
- 125000003275 alpha amino acid group Chemical group 0.000 description 43
- 238000005215 recombination Methods 0.000 description 36
- 230000006798 recombination Effects 0.000 description 35
- 108091034117 Oligonucleotide Proteins 0.000 description 34
- 125000005647 linker group Chemical group 0.000 description 31
- 108091008146 restriction endonucleases Proteins 0.000 description 29
- 238000012217 deletion Methods 0.000 description 27
- 230000037430 deletion Effects 0.000 description 27
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 27
- 230000006870 function Effects 0.000 description 26
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 25
- 230000008569 process Effects 0.000 description 25
- 102000040430 polynucleotide Human genes 0.000 description 24
- 108091033319 polynucleotide Proteins 0.000 description 24
- 239000002157 polynucleotide Substances 0.000 description 24
- 238000004422 calculation algorithm Methods 0.000 description 23
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 22
- 229910052739 hydrogen Inorganic materials 0.000 description 22
- 229920002477 rna polymer Polymers 0.000 description 22
- 238000003786 synthesis reaction Methods 0.000 description 22
- 230000008859 change Effects 0.000 description 20
- 230000035772 mutation Effects 0.000 description 19
- 238000000746 purification Methods 0.000 description 19
- 108060003951 Immunoglobulin Proteins 0.000 description 18
- 102000018358 immunoglobulin Human genes 0.000 description 18
- 239000013612 plasmid Substances 0.000 description 18
- 238000003752 polymerase chain reaction Methods 0.000 description 16
- 238000011282 treatment Methods 0.000 description 16
- 201000010099 disease Diseases 0.000 description 15
- 238000004519 manufacturing process Methods 0.000 description 15
- 230000004048 modification Effects 0.000 description 15
- 238000012986 modification Methods 0.000 description 15
- 239000013615 primer Substances 0.000 description 15
- 102100028310 Immunoglobulin heavy variable 4-31 Human genes 0.000 description 14
- 125000000539 amino acid group Chemical group 0.000 description 14
- 230000001850 reproductive effect Effects 0.000 description 14
- 108700010070 Codon Usage Proteins 0.000 description 13
- 230000006820 DNA synthesis Effects 0.000 description 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 13
- 101000839684 Homo sapiens Immunoglobulin heavy variable 4-31 Proteins 0.000 description 12
- 239000000523 sample Substances 0.000 description 12
- 102000035195 Peptidases Human genes 0.000 description 11
- 108091005804 Peptidases Proteins 0.000 description 11
- 108091093037 Peptide nucleic acid Proteins 0.000 description 11
- 238000007792 addition Methods 0.000 description 11
- 238000005304 joining Methods 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 230000001225 therapeutic effect Effects 0.000 description 11
- 238000010367 cloning Methods 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 10
- 208000035475 disorder Diseases 0.000 description 10
- 239000013604 expression vector Substances 0.000 description 10
- 238000002864 sequence alignment Methods 0.000 description 10
- 208000024891 symptom Diseases 0.000 description 10
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 description 9
- 101150097493 D gene Proteins 0.000 description 9
- 108020004511 Recombinant DNA Proteins 0.000 description 9
- 238000003491 array Methods 0.000 description 9
- 230000000295 complement effect Effects 0.000 description 9
- 239000011159 matrix material Substances 0.000 description 9
- 239000002245 particle Substances 0.000 description 9
- 108090000394 Erythropoietin Proteins 0.000 description 8
- 101150117115 V gene Proteins 0.000 description 8
- 230000004075 alteration Effects 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 230000003993 interaction Effects 0.000 description 8
- 230000001788 irregular Effects 0.000 description 8
- 108091035707 Consensus sequence Proteins 0.000 description 7
- 102000003951 Erythropoietin Human genes 0.000 description 7
- 101150008942 J gene Proteins 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 7
- 239000000556 agonist Substances 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 210000003719 b-lymphocyte Anatomy 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 238000001823 molecular biology technique Methods 0.000 description 7
- 239000011347 resin Substances 0.000 description 7
- 229920005989 resin Polymers 0.000 description 7
- 101710112728 Delta-like protein 4 Proteins 0.000 description 6
- 108010075944 Erythropoietin Receptors Proteins 0.000 description 6
- 241000700605 Viruses Species 0.000 description 6
- 230000004913 activation Effects 0.000 description 6
- 238000001994 activation Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 6
- 239000011324 bead Substances 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 239000012636 effector Substances 0.000 description 6
- 229940105423 erythropoietin Drugs 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 230000001976 improved effect Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 238000002703 mutagenesis Methods 0.000 description 6
- 231100000350 mutagenesis Toxicity 0.000 description 6
- 230000008520 organization Effects 0.000 description 6
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 229910052720 vanadium Inorganic materials 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 239000004471 Glycine Substances 0.000 description 5
- 241000282412 Homo Species 0.000 description 5
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 5
- 238000004590 computer program Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 238000001976 enzyme digestion Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000010348 incorporation Methods 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 150000008300 phosphoramidites Chemical class 0.000 description 5
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 5
- 125000006239 protecting group Chemical group 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 230000014616 translation Effects 0.000 description 5
- 102000005650 Notch Receptors Human genes 0.000 description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical group O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 238000000137 annealing Methods 0.000 description 4
- 239000005557 antagonist Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 230000004071 biological effect Effects 0.000 description 4
- 229920001222 biopolymer Polymers 0.000 description 4
- 239000012707 chemical precursor Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000005547 deoxyribonucleotide Substances 0.000 description 4
- 210000003527 eukaryotic cell Anatomy 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000001390 forced Rayleigh scattering spectroscopy Methods 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 229940072221 immunoglobulins Drugs 0.000 description 4
- 238000007726 management method Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 4
- 210000001236 prokaryotic cell Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 229940124597 therapeutic agent Drugs 0.000 description 4
- 238000004809 thin layer chromatography Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 101001138131 Homo sapiens Immunoglobulin kappa variable 1-8 Proteins 0.000 description 3
- 101001009877 Homo sapiens Immunoglobulin kappa variable 1D-43 Proteins 0.000 description 3
- 101001008257 Homo sapiens Immunoglobulin kappa variable 3D-11 Proteins 0.000 description 3
- 101000956885 Homo sapiens Immunoglobulin lambda variable 2-14 Proteins 0.000 description 3
- 101000978132 Homo sapiens Immunoglobulin lambda variable 7-43 Proteins 0.000 description 3
- 102000003839 Human Proteins Human genes 0.000 description 3
- 108090000144 Human Proteins Proteins 0.000 description 3
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 3
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 3
- 102100020771 Immunoglobulin kappa variable 1-8 Human genes 0.000 description 3
- 102100030883 Immunoglobulin kappa variable 1D-43 Human genes 0.000 description 3
- 102100027405 Immunoglobulin kappa variable 3D-11 Human genes 0.000 description 3
- 102100038429 Immunoglobulin lambda variable 2-14 Human genes 0.000 description 3
- 102100023746 Immunoglobulin lambda variable 7-43 Human genes 0.000 description 3
- 102100039688 Insulin-like growth factor 1 receptor Human genes 0.000 description 3
- 101710184277 Insulin-like growth factor 1 receptor Proteins 0.000 description 3
- 150000008575 L-amino acids Chemical class 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 108700005078 Synthetic Genes Proteins 0.000 description 3
- 230000000890 antigenic effect Effects 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 230000004186 co-expression Effects 0.000 description 3
- 230000021615 conjugation Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 230000002998 immunogenetic effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 238000013507 mapping Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 230000003278 mimic effect Effects 0.000 description 3
- 239000006072 paste Substances 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 239000002987 primer (paints) Substances 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 230000006641 stabilisation Effects 0.000 description 3
- 238000011105 stabilization Methods 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- 238000001308 synthesis method Methods 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 230000003612 virological effect Effects 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- OJHZNMVJJKMFGX-RNWHKREASA-N (4r,4ar,7ar,12bs)-9-methoxy-3-methyl-1,2,4,4a,5,6,7a,13-octahydro-4,12-methanobenzofuro[3,2-e]isoquinoline-7-one;2,3-dihydroxybutanedioic acid Chemical compound OC(=O)C(O)C(O)C(O)=O.O=C([C@@H]1O2)CC[C@H]3[C@]4([H])N(C)CC[C@]13C1=C2C(OC)=CC=C1C4 OJHZNMVJJKMFGX-RNWHKREASA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 2
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 2
- 238000012270 DNA recombination Methods 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 101001037145 Homo sapiens Immunoglobulin heavy variable 2-5 Proteins 0.000 description 2
- 101001008253 Homo sapiens Immunoglobulin kappa variable 3D-7 Proteins 0.000 description 2
- 101001005499 Homo sapiens Probable non-functional immunoglobulin lambda variable 5-48 Proteins 0.000 description 2
- 101000911753 Homo sapiens Protein FAM107B Proteins 0.000 description 2
- 101001012157 Homo sapiens Receptor tyrosine-protein kinase erbB-2 Proteins 0.000 description 2
- 101150024642 IGHV4-31 gene Proteins 0.000 description 2
- 101150062520 IGHV4-34 gene Proteins 0.000 description 2
- 101150047109 IGHV4-39 gene Proteins 0.000 description 2
- 101150049792 IGHV4-4 gene Proteins 0.000 description 2
- 101150071874 IGHV4-59 gene Proteins 0.000 description 2
- 101150068285 IGHV4-61 gene Proteins 0.000 description 2
- 101150101036 IGHV5-51 gene Proteins 0.000 description 2
- 101150024404 IGHV6-1 gene Proteins 0.000 description 2
- 101150025860 IGHV7-81 gene Proteins 0.000 description 2
- 101150037605 IGLV8-61 gene Proteins 0.000 description 2
- 101150035487 IGLV9-49 gene Proteins 0.000 description 2
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 2
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 2
- 102100040235 Immunoglobulin heavy variable 2-5 Human genes 0.000 description 2
- 102100027407 Immunoglobulin kappa variable 3D-7 Human genes 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 102000016979 Other receptors Human genes 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 102100025167 Probable non-functional immunoglobulin lambda variable 5-48 Human genes 0.000 description 2
- 102100026983 Protein FAM107B Human genes 0.000 description 2
- 108020005067 RNA Splice Sites Proteins 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 102100030086 Receptor tyrosine-protein kinase erbB-2 Human genes 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000007720 allelic exclusion Effects 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 229940124691 antibody therapeutics Drugs 0.000 description 2
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 210000000234 capsid Anatomy 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013500 data storage Methods 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 208000037765 diseases and disorders Diseases 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 206010014599 encephalitis Diseases 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 235000013882 gravy Nutrition 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229940022353 herceptin Drugs 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- 239000012678 infectious agent Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000002777 nucleoside Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 150000002894 organic compounds Chemical class 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000008506 pathogenesis Effects 0.000 description 2
- 238000002823 phage display Methods 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000001742 protein purification Methods 0.000 description 2
- 238000001243 protein synthesis Methods 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000002708 random mutagenesis Methods 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 229960004641 rituximab Drugs 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000007781 signaling event Effects 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 230000005945 translocation Effects 0.000 description 2
- 230000017105 transposition Effects 0.000 description 2
- 239000013638 trimer Substances 0.000 description 2
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 2
- 229940045145 uridine Drugs 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 description 1
- 125000001731 2-cyanoethyl group Chemical group [H]C([H])(*)C([H])([H])C#N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- CKTSBUTUHBMZGZ-ULQXZJNLSA-N 4-amino-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-tritiopyrimidin-2-one Chemical compound O=C1N=C(N)C([3H])=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-ULQXZJNLSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010032595 Antibody Binding Sites Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101710158075 Bucky ball Proteins 0.000 description 1
- 101150111062 C gene Proteins 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- XMWRBQBLMFGWIX-UHFFFAOYSA-N C60 fullerene Chemical compound C12=C3C(C4=C56)=C7C8=C5C5=C9C%10=C6C6=C4C1=C1C4=C6C6=C%10C%10=C9C9=C%11C5=C8C5=C8C7=C3C3=C7C2=C1C1=C2C4=C6C4=C%10C6=C9C9=C%11C5=C5C8=C3C3=C7C1=C1C2=C4C6=C2C9=C5C3=C12 XMWRBQBLMFGWIX-UHFFFAOYSA-N 0.000 description 1
- 102100024153 Cadherin-15 Human genes 0.000 description 1
- 101100366043 Caenorhabditis elegans sms-2 gene Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 206010061818 Disease progression Diseases 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
- 241000372837 Fumana Species 0.000 description 1
- 102000003688 G-Protein-Coupled Receptors Human genes 0.000 description 1
- 108090000045 G-Protein-Coupled Receptors Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- BCCRXDTUTZHDEU-VKHMYHEASA-N Gly-Ser Chemical compound NCC(=O)N[C@@H](CO)C(O)=O BCCRXDTUTZHDEU-VKHMYHEASA-N 0.000 description 1
- 241000282575 Gorilla Species 0.000 description 1
- 102100031547 HLA class II histocompatibility antigen, DO alpha chain Human genes 0.000 description 1
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 1
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 1
- 101000762242 Homo sapiens Cadherin-15 Proteins 0.000 description 1
- 101000714553 Homo sapiens Cadherin-3 Proteins 0.000 description 1
- 101000866278 Homo sapiens HLA class II histocompatibility antigen, DO alpha chain Proteins 0.000 description 1
- 101100018617 Homo sapiens IGLL1 gene Proteins 0.000 description 1
- 101000989058 Homo sapiens Immunoglobulin heavy variable 1-69-2 Proteins 0.000 description 1
- 101000989065 Homo sapiens Immunoglobulin heavy variable 7-4-1 Proteins 0.000 description 1
- 101000839685 Homo sapiens Probable non-functional immunoglobulin heavy variable 1-38-4 Proteins 0.000 description 1
- 101000693970 Homo sapiens Scavenger receptor class A member 3 Proteins 0.000 description 1
- 101150050798 IGKV1-39 gene Proteins 0.000 description 1
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 description 1
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 description 1
- 102100029422 Immunoglobulin heavy variable 1-69-2 Human genes 0.000 description 1
- 102100029420 Immunoglobulin heavy variable 7-4-1 Human genes 0.000 description 1
- 102100029616 Immunoglobulin lambda-like polypeptide 1 Human genes 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000000743 Interleukin-5 Human genes 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 102000004310 Ion Channels Human genes 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108700005084 Multigene Family Proteins 0.000 description 1
- 108010021466 Mutant Proteins Proteins 0.000 description 1
- 102000008300 Mutant Proteins Human genes 0.000 description 1
- 108091007491 NSP3 Papain-like protease domains Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 102000002808 Pituitary adenylate cyclase-activating polypeptide Human genes 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 102100028309 Probable non-functional immunoglobulin heavy variable 1-38-4 Human genes 0.000 description 1
- 108010029485 Protein Isoforms Proteins 0.000 description 1
- 102000001708 Protein Isoforms Human genes 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 241000282849 Ruminantia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 102100027188 Thyroid peroxidase Human genes 0.000 description 1
- 101710120037 Toxin CcdB Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 208000009956 adenocarcinoma Diseases 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000009824 affinity maturation Effects 0.000 description 1
- 150000001371 alpha-amino acids Chemical class 0.000 description 1
- 235000008206 alpha-amino acids Nutrition 0.000 description 1
- 230000001668 ameliorated effect Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- 229940125644 antibody drug Drugs 0.000 description 1
- 229940125752 antibody drug candidate Drugs 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 210000004507 artificial chromosome Anatomy 0.000 description 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 238000012550 audit Methods 0.000 description 1
- 229940120638 avastin Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 229960000397 bevacizumab Drugs 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000002843 carboxylic acid group Chemical group 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000001193 catalytic steam reforming Methods 0.000 description 1
- 238000000423 cell based assay Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000004182 chemical digestion Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000000039 congener Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000000412 dendrimer Substances 0.000 description 1
- 229920000736 dendritic polymer Polymers 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011033 desalting Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000005750 disease progression Effects 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 125000002228 disulfide group Chemical group 0.000 description 1
- 238000013104 docking experiment Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000002825 functional assay Methods 0.000 description 1
- 210000002980 germ line cell Anatomy 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000003966 growth inhibitor Substances 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000002169 hydrotherapy Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 229960000598 infliximab Drugs 0.000 description 1
- 230000010365 information processing Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000007854 ligation-mediated PCR Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 238000002824 mRNA display Methods 0.000 description 1
- 230000005291 magnetic effect Effects 0.000 description 1
- 210000000723 mammalian artificial chromosome Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000000302 molecular modelling Methods 0.000 description 1
- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 238000004801 process automation Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 229940116176 remicade Drugs 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000002702 ribosome display Methods 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000010845 search algorithm Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000037432 silent mutation Effects 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000012306 spectroscopic technique Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003883 substance clean up Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 210000001179 synovial fluid Anatomy 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 150000003536 tetrazoles Chemical class 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 229960000575 trastuzumab Drugs 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2869—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against hormone receptors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2884—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD44
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/515—Complete light chain, i.e. VL + CL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/73—Inducing cell death, e.g. apoptosis, necrosis or inhibition of cell proliferation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/74—Inducing cell proliferation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Endocrinology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Neurology (AREA)
- Structural Engineering (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Oncology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19876408P | 2008-11-07 | 2008-11-07 | |
| US61/198,764 | 2008-11-07 | ||
| US21120409P | 2009-03-25 | 2009-03-25 | |
| US61/211,204 | 2009-03-25 | ||
| PCT/US2009/063299 WO2010054007A1 (en) | 2008-11-07 | 2009-11-04 | Combinatorial antibody libraries and uses thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016018531A Division JP2016127850A (ja) | 2008-11-07 | 2016-02-03 | 組合せ抗体ライブラリー及びその使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2012508016A JP2012508016A (ja) | 2012-04-05 |
| JP2012508016A5 JP2012508016A5 (enExample) | 2013-01-24 |
| JP5882058B2 true JP5882058B2 (ja) | 2016-03-09 |
Family
ID=41508157
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011535649A Expired - Fee Related JP5882058B2 (ja) | 2008-11-07 | 2009-11-04 | 組合せ抗体ライブラリー及びその使用 |
| JP2011535651A Pending JP2012508017A (ja) | 2008-11-07 | 2009-11-04 | 抗dll4抗体及びその使用 |
| JP2015157842A Pending JP2016040246A (ja) | 2008-11-07 | 2015-08-10 | 抗dll4抗体及びその使用 |
| JP2016018531A Pending JP2016127850A (ja) | 2008-11-07 | 2016-02-03 | 組合せ抗体ライブラリー及びその使用 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011535651A Pending JP2012508017A (ja) | 2008-11-07 | 2009-11-04 | 抗dll4抗体及びその使用 |
| JP2015157842A Pending JP2016040246A (ja) | 2008-11-07 | 2015-08-10 | 抗dll4抗体及びその使用 |
| JP2016018531A Pending JP2016127850A (ja) | 2008-11-07 | 2016-02-03 | 組合せ抗体ライブラリー及びその使用 |
Country Status (7)
| Country | Link |
|---|---|
| US (4) | US9221902B2 (enExample) |
| EP (2) | EP2356270B1 (enExample) |
| JP (4) | JP5882058B2 (enExample) |
| AU (4) | AU2009313560B2 (enExample) |
| CA (2) | CA2742968C (enExample) |
| DK (1) | DK2356270T3 (enExample) |
| WO (2) | WO2010054010A1 (enExample) |
Families Citing this family (203)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9567396B2 (en) | 2006-03-07 | 2017-02-14 | Evonik Degussa Gmbh | Notch inhibition in the prevention of vein graft failure |
| WO2011053822A2 (en) | 2009-11-01 | 2011-05-05 | The Brigham And Women's Hospital, Inc. | Notch inhibition in the treatment and prevention of obesity and metabolic syndrome |
| HUE028379T2 (en) | 2006-09-29 | 2016-12-28 | Oncomed Pharm Inc | Preparations and procedures for the diagnosis and treatment of cancer |
| US8420779B2 (en) | 2007-05-22 | 2013-04-16 | Amgen Inc. | Compositions and methods for producing bioactive fusion proteins |
| KR20100126811A (ko) | 2008-03-14 | 2010-12-02 | 바이오콘 리미티드 | 모노클로날 항체 및 그것의 방법 |
| JP5882058B2 (ja) | 2008-11-07 | 2016-03-09 | ファブラス エルエルシー | 組合せ抗体ライブラリー及びその使用 |
| EP3385279B1 (en) | 2009-03-20 | 2020-02-26 | Amgen Inc. | Carrier immunoglobulins and uses thereof |
| WO2010121370A1 (en) * | 2009-04-20 | 2010-10-28 | University Health Network | Prognostic gene expression signature for squamous cell carcinoma of the lung |
| ES2511051T3 (es) | 2009-05-29 | 2014-10-22 | Morphosys Ag | Colección de anticuerpos sintéticos para tratar enfermedades |
| TWI513465B (zh) * | 2009-06-25 | 2015-12-21 | Regeneron Pharma | 以dll4拮抗劑與化學治療劑治療癌症之方法 |
| TR201818814T4 (tr) | 2009-10-16 | 2019-01-21 | Oncomed Pharm Inc | Dll4 antagonist antikorlarının ve anti-hipertansif ajanların terapötik kombinasyonu ve kullanımı. |
| EP2496600A1 (en) | 2009-11-04 | 2012-09-12 | Fabrus LLC | Methods for affinity maturation-based antibody optimization |
| UA109888C2 (uk) | 2009-12-07 | 2015-10-26 | ІЗОЛЬОВАНЕ АНТИТІЛО АБО ЙОГО ФРАГМЕНТ, ЩО ЗВ'ЯЗУЄТЬСЯ З β-КЛОТО, РЕЦЕПТОРАМИ FGF І ЇХНІМИ КОМПЛЕКСАМИ | |
| US10016620B2 (en) * | 2010-02-04 | 2018-07-10 | Procept Biorobotics Incorporation | Tissue sampling and cancer treatment apparatus |
| US20130045492A1 (en) | 2010-02-08 | 2013-02-21 | Regeneron Pharmaceuticals, Inc. | Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain |
| US20120021409A1 (en) * | 2010-02-08 | 2012-01-26 | Regeneron Pharmaceuticals, Inc. | Common Light Chain Mouse |
| US9796788B2 (en) | 2010-02-08 | 2017-10-24 | Regeneron Pharmaceuticals, Inc. | Mice expressing a limited immunoglobulin light chain repertoire |
| NZ631363A (en) | 2010-02-08 | 2016-05-27 | Regeneron Pharma | Common light chain mouse |
| US9354228B2 (en) | 2010-07-16 | 2016-05-31 | Adimab, Llc | Antibody libraries |
| CA2806076A1 (en) | 2010-07-22 | 2012-01-26 | The Regents Of The University Of California | Anti-tumor antigen antibodies and methods of use |
| US8551479B2 (en) | 2010-09-10 | 2013-10-08 | Oncomed Pharmaceuticals, Inc. | Methods for treating melanoma |
| KR20130110169A (ko) | 2010-09-22 | 2013-10-08 | 암젠 인크 | 담체 면역글로뷸린 및 이것의 용도 |
| SG189950A1 (en) | 2010-11-19 | 2013-06-28 | Morphosys Ag | A collection and methods for its use |
| US20160272697A2 (en) * | 2011-04-28 | 2016-09-22 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Neutralizing Antibodies to Nipah and Hendra Virus |
| CA3078215A1 (en) * | 2011-04-28 | 2012-11-01 | Department Of Veterans Affairs | Identification of polynucleotides associated with a sample |
| CA3025762C (en) * | 2011-05-04 | 2021-11-09 | Omeros Corporation | Compositions for inhibiting masp-2 dependent complement activation |
| CN103596984B (zh) * | 2011-06-15 | 2016-04-13 | 霍夫曼-拉罗奇有限公司 | 抗-人epo受体的抗体及使用方法 |
| DK2739740T3 (da) | 2011-08-05 | 2019-12-09 | Regeneron Pharma | Universelle, humaniserede letkæde-mus |
| DK2744931T3 (en) * | 2011-08-18 | 2018-08-06 | Affinity Biosciences Pty Ltd | SOLUBLE POLYPEPTIDES |
| ES2707580T3 (es) | 2011-09-23 | 2019-04-04 | Oncomed Pharm Inc | Agentes de unión a VEGF/DLL4 y usos de los mismos |
| WO2013067055A1 (en) | 2011-11-01 | 2013-05-10 | Bionomics, Inc. | Methods of blocking cancer stem cell growth |
| EP2773665A1 (en) | 2011-11-01 | 2014-09-10 | Bionomics, Inc. | Antibodies and methods of treating cancer |
| AU2013208003B2 (en) | 2012-01-09 | 2017-12-14 | The Scripps Research Institute | Ultralong complementarity determining regions and uses thereof |
| EP2802601B1 (en) | 2012-01-09 | 2019-11-13 | The Scripps Research Institute | Humanized antibodies with ultralong cdr3s |
| US9150648B2 (en) * | 2012-01-31 | 2015-10-06 | Regeneron Pharmaceuticals, Inc. | Anti-ASIC1 antibodies and uses thereof |
| WO2013131074A1 (en) * | 2012-03-02 | 2013-09-06 | Diogenix, Inc. | Methods and reagents for evaluating autoimmune disease and determining antibody repertoire |
| WO2013134743A1 (en) | 2012-03-08 | 2013-09-12 | Halozyme, Inc. | Conditionally active anti-epidermal growth factor receptor antibodies and methods of use thereof |
| CA2875543A1 (en) * | 2012-06-21 | 2013-12-27 | Sorrento Therapeutics, Inc. | Antigen binding proteins that bind igf1r |
| KR101535341B1 (ko) * | 2012-07-02 | 2015-07-13 | 한화케미칼 주식회사 | Dll4에 특이적으로 결합하는 신규한 단일클론항체 및 이의 용도 |
| US9777067B2 (en) | 2012-09-27 | 2017-10-03 | Massachusetts Institute Of Technology | HER2- and VEGF-A-binding proteins with enhanced stability |
| US9309318B2 (en) * | 2012-10-17 | 2016-04-12 | Amgen, Inc. | Compositions relating to anti-IL-21 receptor antibodies |
| WO2014071018A1 (en) * | 2012-10-31 | 2014-05-08 | Oncomed Pharmaceuticals, Inc. | Methods and monitoring of treatment with a dll4 antagonist |
| WO2014074528A2 (en) * | 2012-11-06 | 2014-05-15 | Medimmune, Llc | Combination therapies using anti-pseudomonas psl and pcrv binding molecules |
| WO2014085821A2 (en) * | 2012-11-30 | 2014-06-05 | The Regents Of The University Of California | Fully human antibodies and fragments recognizing human c-met |
| LT2928921T (lt) | 2012-12-05 | 2021-04-26 | Novartis Ag | Kompozicijos ir būdai, skirti antikūnams nukreipti į epo |
| WO2014116051A1 (ko) | 2013-01-24 | 2014-07-31 | 스크립스코리아 항체연구원 | 단백질 조합 기반의 Fv 라이브러리 및 이의 제조 방법 |
| US9896685B2 (en) | 2013-04-25 | 2018-02-20 | Sutro Biopharma, Inc. | Use of LAMBDA-GAM protein in ribosomal display technology |
| US20160069897A1 (en) * | 2013-04-25 | 2016-03-10 | Suthro Biopharma, Inc. | Selection of fab fragments using ribosomal display technology |
| RU2539752C2 (ru) * | 2013-05-23 | 2015-01-27 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | ГУМАНИЗИРОВАННОЕ АНТИТЕЛО И АНТИГЕНСВЯЗЫВАЮЩИЙ ФРАГМЕНТ (Fab), СВЯЗЫВАЮЩИЕСЯ С ИНТЕРФЕРОНОМ- γ ЧЕЛОВЕКА, ФРАГМЕНТЫ ДНК, КОДИРУЮЩИЕ УКАЗАННОЕ АНТИТЕЛО И АНТИГЕНСВЯЗЫВАЮЩИЙ ФРАГМЕНТ, КЛЕТКА, ТРАНСФОРМИРОВАННАЯ ФРАГМЕНТОМ ДНК, И СПОСОБ ПОЛУЧЕНИЯ УКАЗАННОГО АНТИТЕЛА И АНТИГЕНСВЯЗЫВАЮЩЕГО ФРАГМЕНТА |
| US10208125B2 (en) * | 2013-07-15 | 2019-02-19 | University of Pittsburgh—of the Commonwealth System of Higher Education | Anti-mucin 1 binding agents and uses thereof |
| WO2015017146A2 (en) | 2013-07-18 | 2015-02-05 | Fabrus, Inc. | Antibodies with ultralong complementarity determining regions |
| CA2918370A1 (en) | 2013-07-18 | 2015-01-22 | Fabrus, Inc. | Humanized antibodies with ultralong complementarity determining regions |
| PL3052192T3 (pl) * | 2013-10-02 | 2021-04-06 | Medimmune, Llc | Przeciwciała neutralizujące przeciwko grypie A i ich zastosowania |
| WO2015057906A1 (en) * | 2013-10-16 | 2015-04-23 | Janssen Biotech, Inc. | Cd200 receptor 1 agonists |
| GB201403775D0 (en) * | 2014-03-04 | 2014-04-16 | Kymab Ltd | Antibodies, uses & methods |
| ES2897765T3 (es) * | 2014-03-14 | 2022-03-02 | Hoffmann La Roche | Procedimientos y composiciones para la secreción de polipéptidos heterólogos |
| KR102601491B1 (ko) | 2014-03-21 | 2023-11-13 | 리제너론 파마슈티칼스 인코포레이티드 | 단일 도메인 결합 단백질을 생산하는 비-인간 동물 |
| CN106459213B (zh) | 2014-04-03 | 2020-12-01 | Igm生物科学股份有限公司 | 修饰的j链 |
| EP3126397B1 (en) | 2014-04-04 | 2020-01-29 | Bionomics, Inc. | Humanized antibodies that bind lgr5 |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| EP3160566B1 (en) * | 2014-06-24 | 2020-03-11 | Procept Biorobotics Corporation | Tissue sampling and cancer treatment apparatus |
| CN113563462B (zh) | 2014-07-15 | 2024-08-13 | 免疫医疗有限责任公司 | 中和抗乙型流感抗体及其用途 |
| EP3185897A4 (en) * | 2014-08-29 | 2018-05-02 | Sorrento Therapeutics, Inc. | Antibody therapeutics that bind oprf and opri |
| GB201419094D0 (en) | 2014-10-27 | 2014-12-10 | Agency Science Tech & Res | Anti-TIM-3-antibodies |
| JP6709215B2 (ja) | 2014-10-27 | 2020-06-10 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | 抗tim−3抗体 |
| WO2016070051A2 (en) | 2014-10-31 | 2016-05-06 | Oncomed Pharmaceuticals, Inc. | Combination therapy for treatment of disease |
| TW201625692A (zh) | 2014-11-14 | 2016-07-16 | 諾華公司 | 抗體藥物結合物 |
| EP3026060A1 (en) * | 2014-11-26 | 2016-06-01 | Miltenyi Biotec GmbH | Humanized antibody or fragment thereof specific for CD45R0 |
| SG11201704549UA (en) | 2014-12-05 | 2017-07-28 | Memorial Sloan Kettering Cancer Center | Chimeric antigen receptors targeting b-cell maturation antigen and uses thereof |
| CN113388036A (zh) | 2014-12-05 | 2021-09-14 | 纪念斯隆-凯特琳癌症中心 | 靶向b-细胞成熟抗原的抗体及其用途 |
| EP3246434A1 (en) * | 2015-01-13 | 2017-11-22 | Ewha University-Industry Collaboration Foundation | Method for preparing novel antibody library and library prepared thereby |
| MX2017011600A (es) | 2015-03-10 | 2018-06-13 | Sorrento Therapeutics Inc | Terapeuticos de anticuerpo que enlazan antigeno de membrana especifico de prostata. |
| CA2979702A1 (en) | 2015-03-19 | 2016-09-22 | Regeneron Pharmaceuticals, Inc. | Non-human animals that select for light chain variable regions that bind antigen |
| EP3280443B1 (en) | 2015-04-08 | 2022-08-24 | Sorrento Therapeutics, Inc. | Antibody therapeutics that bind cd38 |
| AR104309A1 (es) | 2015-04-17 | 2017-07-12 | Bayer Pharma AG | Construcciones de anticuerpos biespecíficos para cdh3 y cd3 |
| KR20180015650A (ko) | 2015-05-07 | 2018-02-13 | 아게누스 인코포레이티드 | 항-ox40 항체 및 이의 사용 방법 |
| US10787517B2 (en) | 2015-05-18 | 2020-09-29 | Agensys, Inc. | Antibodies that bind to AXL proteins |
| EP3297662A4 (en) | 2015-05-18 | 2019-03-13 | Agensys, Inc. | ANTIBODIES BINDING TO AXL PROTEINS |
| LT3303384T (lt) | 2015-06-01 | 2021-10-11 | Medimmune, Llc | Neutralizuojančios surišančios molekulės prieš gripą ir jų naudojimas |
| US20180155411A1 (en) * | 2015-07-08 | 2018-06-07 | The Board Of Trustees Of The Leland Stanford Junior University | Heterotypic antibodies specific for human rotavirus |
| CN107922975B (zh) | 2015-08-12 | 2022-06-28 | 诺华股份有限公司 | 治疗眼科病症的方法 |
| JP6967003B2 (ja) | 2015-09-23 | 2021-11-17 | メレオ バイオファーマ 5 インコーポレイテッド | がんの処置のための方法および組成物 |
| DK3356401T3 (da) | 2015-09-30 | 2020-09-07 | Igm Biosciences Inc | Bindingsmolekyler med modificeret j-kæde |
| EP3824903A1 (en) | 2015-09-30 | 2021-05-26 | IGM Biosciences Inc. | Binding molecules with modified j-chain |
| TWI752920B (zh) | 2015-10-12 | 2022-01-21 | 美商再生元醫藥公司 | 活化瘦素受體的抗原結合蛋白 |
| EP3184547A1 (en) * | 2015-10-29 | 2017-06-28 | F. Hoffmann-La Roche AG | Anti-tpbg antibodies and methods of use |
| HUE050312T2 (hu) | 2015-11-10 | 2020-11-30 | Medimmune Llc | ASCT2-RE specifikus kötõmolekulák és alkalmazásaik |
| EP3383914A4 (en) * | 2015-12-02 | 2019-10-30 | Agenus Inc. | ANTI-OX40 ANTIBODIES AND METHOD OF USE THEREOF |
| CA3007233A1 (en) | 2015-12-02 | 2017-06-08 | Agenus Inc. | Antibodies and methods of use thereof |
| WO2017096182A1 (en) * | 2015-12-03 | 2017-06-08 | Agenus Inc. | Anti-ox40 antibodies and methods of use thereof |
| CN106939047B (zh) * | 2016-01-04 | 2021-08-31 | 江苏怀瑜药业有限公司 | 一种pd-l1抗体及其制备方法 |
| CN105693821A (zh) * | 2016-01-08 | 2016-06-22 | 中国药科大学 | 与人血管内皮生长因子受体-3(vegfr-3)蛋白特异性结合的多肽,其筛选方法,鉴定和用途 |
| US11136407B2 (en) * | 2016-01-08 | 2021-10-05 | Aimm Therapeutics B.V. | Therapeutic anti-CD9 antibody |
| IL304940A (en) | 2016-01-13 | 2023-10-01 | Medimmune Llc | A method for treating type A influenza |
| JP7022067B2 (ja) * | 2016-01-14 | 2022-02-17 | メモリアル スローン ケタリング キャンサー センター | Foxp3由来のペプチドに特異的なt細胞受容体様抗体 |
| KR20180138205A (ko) | 2016-03-22 | 2018-12-28 | 바이오노믹스 리미티드 | 항-lgr5 단클론성 항체의 투여 |
| US11197937B2 (en) * | 2016-04-15 | 2021-12-14 | The Trustees Of The University Of Pennsylvania | Compositions for treatment of wet age-related macular degeneration |
| MX2018015757A (es) * | 2016-06-14 | 2019-05-30 | Merck Sharp & Dohme | Anticuerpos antifactor de la coagulacion xi. |
| IL263834B2 (en) | 2016-06-20 | 2024-01-01 | Kymab Ltd | Anti-pd-l1 antibodies |
| JP6987134B2 (ja) | 2016-06-27 | 2021-12-22 | ジュノー セラピューティクス インコーポレイテッド | ペプチドエピトープを同定する方法、そのようなエピトープに結合する分子および関連用途 |
| MA45491A (fr) | 2016-06-27 | 2019-05-01 | Juno Therapeutics Inc | Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés |
| EP3510046A4 (en) * | 2016-09-07 | 2020-05-06 | The Regents of the University of California | ANTIBODIES AGAINST OXIDATION-SPECIFIC EPITOPES |
| CN118005799A (zh) * | 2016-09-23 | 2024-05-10 | 马伦戈治疗公司 | 包含λ轻链和κ轻链的多特异性抗体分子 |
| US10738338B2 (en) | 2016-10-18 | 2020-08-11 | The Research Foundation for the State University | Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate |
| US11667697B2 (en) | 2016-10-19 | 2023-06-06 | Vanderbilt University | Human orthopoxvirus antibodies and methods of use therefor |
| WO2018081754A1 (en) * | 2016-10-29 | 2018-05-03 | University Of Miami | Anti-zika virus antibodies |
| WO2018083248A1 (en) | 2016-11-03 | 2018-05-11 | Kymab Limited | Antibodies, combinations comprising antibodies, biomarkers, uses & methods |
| ES3040144T3 (en) | 2016-11-08 | 2025-10-28 | Regeneron Pharma | Antigen-binding proteins that antagonize leptin receptor |
| WO2018089628A1 (en) * | 2016-11-09 | 2018-05-17 | Agenus Inc. | Anti-ox40 antibodies, anti-gitr antibodies, and methods of use thereof |
| TW201832778A (zh) * | 2016-11-10 | 2018-09-16 | 美商麥迪紐有限責任公司 | 對asct2具有特異性的結合分子及其用途 |
| BR112019011186A2 (pt) | 2016-12-01 | 2019-10-08 | Regeneron Pharmaceuticals, Inc. | conjugado de anticorpo radiomarcado, composto, e, métodos de imageamento e para tratamento de um tumor. |
| SI3555132T1 (sl) * | 2016-12-19 | 2024-04-30 | Medimmune Limited | Protitelesa proti LIF in njihove uporabe |
| KR20190118172A (ko) | 2017-02-08 | 2019-10-17 | 드래곤플라이 쎄라퓨틱스, 인크. | 천연 킬러 세포의 활성화를 위한 다중-특이적 결합 단백질 및 암 치료에서의 그의 치료적 용도 |
| CN108456250B (zh) * | 2017-02-17 | 2025-11-28 | 恺兴生命科技(上海)有限公司 | 靶向il-13ra2的抗体及其应用 |
| CA3052779A1 (en) | 2017-02-17 | 2018-08-23 | Fred Hutchinson Cancer Research Center | Combination therapies for treatment of bcma-related cancers and autoimmune disorders |
| EP4273258A3 (en) | 2017-02-20 | 2024-01-17 | Dragonfly Therapeutics, Inc. | Proteins binding her2, nkg2d and cd16 |
| CN110637031B (zh) * | 2017-03-04 | 2024-04-16 | 湘潭腾华生物科技有限公司 | 程序性死亡蛋白1(pd-1)的重组抗体及其用途 |
| JOP20180021A1 (ar) * | 2017-03-16 | 2019-01-30 | Janssen Biotech Inc | الأجسام المضادة لتكتلات خيوط بروتين تاو (tau) الحلزونية المزدوجة واستخداماتها |
| US11879014B2 (en) | 2017-03-17 | 2024-01-23 | Tusk Therapeutics Ltd. | Method of treating cancer or depleting regulatory T cells in a subject by administering a human IGG1 anti-CD25 antibody |
| US10296425B2 (en) | 2017-04-20 | 2019-05-21 | Bank Of America Corporation | Optimizing data processing across server clusters and data centers using checkpoint-based data replication |
| US11976121B2 (en) | 2017-07-20 | 2024-05-07 | H. Lee Moffitt Cancer Center And Research Institute, Inc. | CD123-binding chimeric antigen receptors |
| IL316357A (en) * | 2017-07-31 | 2024-12-01 | Trishula Therapeutics Inc | Anti-cd39 antibodies, compositions comprising anti-cd39 antibodies and methods of using anti-cd39 antibodies |
| CA3072816A1 (en) * | 2017-08-18 | 2019-02-21 | Gritstone Oncology, Inc. | Antigen-binding proteins targeting shared antigens |
| KR20200074997A (ko) | 2017-11-01 | 2020-06-25 | 주노 쎄러퓨티크스 인코퍼레이티드 | B-세포 성숙 항원에 특이적인 항체 및 키메릭 항원 수용체 |
| DK3703750T3 (da) | 2017-11-01 | 2025-02-10 | Juno Therapeutics Inc | Kimæriske antigenreceptorer, der er specifikke for b-cellemodningsantigen, og som koder for polynukleotider |
| KR20200099137A (ko) | 2017-11-06 | 2020-08-21 | 주노 쎄러퓨티크스 인코퍼레이티드 | 세포 요법 및 감마 세크레타제 억제제의 조합 |
| JP7291700B2 (ja) | 2017-11-20 | 2023-06-15 | ナントバイオ,インコーポレイテッド | mRNAディスプレイ抗体ライブラリー及び方法 |
| US11034951B2 (en) | 2017-11-20 | 2021-06-15 | Nantbio, Inc. | mRNA display antibody library and methods |
| KR20200100089A (ko) | 2017-12-18 | 2020-08-25 | 리제너론 파마슈티칼스 인코포레이티드 | 렙틴 수용체 및/또는 gp130에 결합하는 이중 특이적 항원 결합 분자 및 이의 사용 방법 |
| AU2018393074B2 (en) | 2017-12-19 | 2025-10-23 | Surrozen Operating, Inc. | Anti-frizzled antibodies and methods of use |
| US11746150B2 (en) | 2017-12-19 | 2023-09-05 | Surrozen Operating, Inc. | Anti-LRP5/6 antibodies and methods of use |
| CN111727203B (zh) | 2017-12-19 | 2024-04-26 | 瑟罗泽恩奥普瑞汀公司 | Wnt替代分子和其用途 |
| WO2019143884A1 (en) * | 2018-01-19 | 2019-07-25 | Vanderbilt University | Conserved hiv antibody clonotypes and methods of use |
| WO2019157366A1 (en) | 2018-02-08 | 2019-08-15 | Dragonfly Therapeutics, Inc. | Antibody variable domains targeting the nkg2d receptor |
| MX2020008333A (es) | 2018-02-08 | 2020-09-21 | Dragonfly Therapeutics Inc | Terapia de combinacion del cancer que incluye proteinas de union multiespecificas que activan a las celulas asesinas naturales. |
| MX2020008684A (es) * | 2018-02-20 | 2020-12-07 | Dragonfly Therapeutics Inc | Proteinas de union multiespecificas que se unen a cd33, nkg2d y cd16 y metodos de uso. |
| AU2019232631A1 (en) | 2018-03-05 | 2020-09-24 | Janssen Pharmaceutica Nv | Anti-PHF-tau antibodies and uses thereof |
| TW201940515A (zh) * | 2018-03-13 | 2019-10-16 | 英商塔斯克療法有限公司 | 抗-cd25抗體藥劑 |
| WO2019195796A1 (en) | 2018-04-06 | 2019-10-10 | Regeneron Pharmaceuticals, Inc. | A leptin receptor agonist antibody for use in treating a metabolic dysfunction or hypoleptinemia |
| EP3553082A1 (en) * | 2018-04-12 | 2019-10-16 | Bayer Aktiengesellschaft | Brain natriuretic peptide engrafted antibodies |
| EP3553081A1 (en) | 2018-04-12 | 2019-10-16 | Bayer Aktiengesellschaft | Atrial natriuretic peptide engrafted antibodies |
| EP3553079A1 (en) * | 2018-04-12 | 2019-10-16 | Bayer Aktiengesellschaft | C-type natriuretic peptide engrafted antibodies |
| WO2020010235A1 (en) * | 2018-07-05 | 2020-01-09 | H. Lee Moffitt Cancer Center And Research Institute Inc. | Car t cells that target b-cell antigens |
| WO2020014143A1 (en) | 2018-07-08 | 2020-01-16 | Specifica Inc. | Antibody libraries with maximized antibody developability characteristics |
| CN110713537B (zh) * | 2018-07-13 | 2023-04-21 | 江苏怀瑜药业有限公司 | 一种sema4d抗体及其制备方法和应用 |
| EA202091888A1 (ru) | 2018-08-08 | 2020-10-23 | Драгонфлай Терапьютикс, Инк. | Вариабельные домены антител, нацеленные на рецептор nkg2d |
| MX2021001527A (es) | 2018-08-08 | 2021-06-15 | Dragonfly Therapeutics Inc | Proteínas de unión a nkg2d, cd16 y a un antígeno asociado a tumor. |
| CA3108427A1 (en) | 2018-08-08 | 2020-02-13 | Dragonfly Therapeutics, Inc. | Multi-specific binding proteins that bind bcma, nkg2d and cd16, and methods of use |
| EP3844266A1 (en) | 2018-08-28 | 2021-07-07 | Fred Hutchinson Cancer Research Center | Methods and compositions for adoptive t cell therapy incorporating induced notch signaling |
| EP3873943A2 (en) | 2018-11-01 | 2021-09-08 | Juno Therapeutics, Inc. | Methods for treatment using chimeric antigen receptors specific for b-cell maturation antigen |
| CN109553680B (zh) * | 2018-12-12 | 2021-06-01 | 广州市第八人民医院 | 一种单克隆抗体ZKns4B8及其应用 |
| BR112021012569A2 (pt) * | 2018-12-28 | 2021-09-14 | Kyowa Kirin Co., Ltd. | Anticorpo biespecífico de ligação ao tfr |
| JP7550770B2 (ja) * | 2019-01-31 | 2024-09-13 | ナントバイオ,インコーポレイテッド | mRNAディスプレイ抗体ライブラリー及び方法 |
| SG11202109057XA (en) | 2019-03-05 | 2021-09-29 | Nkarta Inc | Cd19-directed chimeric antigen receptors and uses thereof in immunotherapy |
| US12479908B2 (en) | 2019-03-29 | 2025-11-25 | H. Lee Moffitt Cancer Center And Research Institute, Inc. | Antibodies against programmed cell death protein 1 (PD1) and uses thereof |
| EP3959242A4 (en) * | 2019-04-24 | 2023-09-13 | Magenta Therapeutics, Inc. | ANTRACYCLINE ANTIBODY-DRUG CONJUGATES AND THEIR USES |
| GB2600274B (en) | 2019-06-07 | 2024-11-27 | Scribe Therapeutics Inc | Engineered CasX systems |
| WO2020247883A2 (en) * | 2019-06-07 | 2020-12-10 | Scribe Therapeutics Inc. | Deep mutational evolution of biomolecules |
| US12448462B2 (en) | 2019-06-14 | 2025-10-21 | Dana-Farber Cancer Institute, Inc. | Antibodies against MUC1 |
| AU2020300618A1 (en) * | 2019-07-02 | 2022-01-27 | Surrozen Operating, Inc. | Monospecific anti-frizzled antibodies and methods of use |
| EP3999532A2 (en) * | 2019-07-16 | 2022-05-25 | Sanofi | Neutralizing anti-amyloid beta antibodies for the treatment of alzheimer's disease |
| CN115003697A (zh) | 2019-09-23 | 2022-09-02 | 特韦斯特生物科学公司 | Crth2的变异核酸文库 |
| US12173282B2 (en) * | 2019-09-23 | 2024-12-24 | Twist Bioscience, Inc. | Antibodies that bind CD3 epsilon |
| BR112022007720A2 (pt) * | 2019-10-24 | 2022-08-23 | Prometheus Biosciences Inc | Anticorpos humanizados para ligante 1a tnf-like (tl1a) e seus usos |
| WO2021202863A1 (en) * | 2020-04-02 | 2021-10-07 | Promab Biotechnologies, Inc. | Human ror-1 antibody and anti-ror-1-car-t cells |
| JP2023523336A (ja) * | 2020-04-27 | 2023-06-02 | ツイスト バイオサイエンス コーポレーション | コロナウイルス用の変異体核酸ライブラリー |
| EP4146271A4 (en) | 2020-05-06 | 2024-09-04 | Dragonfly Therapeutics, Inc. | PROTEINS THAT BIND NKG2D, CD16 AND CLEC12A |
| AU2021277373A1 (en) * | 2020-05-20 | 2023-01-05 | The Trustees Of Columbia University In The City Of New York | Potent neutralizing antibodies against SARS-CoV-2, generation and uses thereof |
| CN111748033B (zh) * | 2020-05-27 | 2021-02-02 | 江苏省疾病预防控制中心(江苏省公共卫生研究院) | 一种与新型冠状病毒np蛋白结合的分离抗体、包含其的检测试剂盒 |
| IL298510A (en) | 2020-06-12 | 2023-01-01 | Nkarta Inc | Genetically modified natural killer cells for cd70-directed cancer immunotherapy |
| TWI806088B (zh) * | 2020-06-30 | 2023-06-21 | 大陸商和鉑醫藥(上海)有限責任公司 | 具有H2L2與HCAb結構的結合蛋白 |
| AU2021324615A1 (en) * | 2020-08-10 | 2023-03-09 | Integral Molecular, Inc. | Methods and compositions for making antibody libraries and antibodies isolated from the same |
| US20230391856A1 (en) * | 2020-10-23 | 2023-12-07 | Icahn School Of Medicine At Mount Sinai | Sars-cov-2 antibodies and uses thereof |
| EP4256054A1 (en) | 2020-12-03 | 2023-10-11 | Scribe Therapeutics Inc. | Engineered class 2 type v crispr systems |
| JP2023552762A (ja) * | 2020-12-04 | 2023-12-19 | エクイリウム,インコーポレイテッド | Cd6high細胞を選択的に標的化してteff細胞の活性を低下する方法 |
| CN117203228A (zh) * | 2021-01-11 | 2023-12-08 | 阿迪马布有限责任公司 | 被工程化为优先进行链配对的变体ch1结构域和变体cl结构域以及包括所述变体ch1结构域和所述变体cl结构域的多特异性抗体 |
| EP4301774A4 (en) | 2021-03-03 | 2025-08-13 | Dragonfly Therapeutics Inc | METHODS OF TREATING CANCER USING MULTI-SPECIFIC BINDING PROTEINS THAT BIND TO NKG2D, CD16, AND A TUMOR-ASSOCIATED ANTIGEN |
| US20240327503A1 (en) * | 2021-03-12 | 2024-10-03 | Jimmy Dale GOLLIHAR | Sars-cov-2 neutralizing antibodies and uses thereof |
| WO2022204316A2 (en) | 2021-03-24 | 2022-09-29 | Twist Bioscience Corporation | Variant nucleic acid libraries for cd3 |
| WO2022266383A1 (en) * | 2021-06-17 | 2022-12-22 | Atreca, Inc. | Anti-csp antibodies |
| JP2024526324A (ja) * | 2021-07-12 | 2024-07-17 | ンカルタ・インコーポレイテッド | Bcma指向性細胞免疫療法の組成物および方法 |
| CA3227150A1 (en) * | 2021-07-23 | 2023-01-26 | The Trustees Of Columbia University In The City Of New York | Characterization of potent and broadly neutralizing monoclonal antibodies against sars-cov-2, its variants, and related coronaviruses and methods of use |
| JP2024527029A (ja) | 2021-07-29 | 2024-07-19 | セファロン リミティド ライアビリティ カンパニー | 抗pacap抗体の組成物及び方法 |
| US11987641B2 (en) | 2021-08-27 | 2024-05-21 | Janssen Biotech, Inc. | Anti-PSMA antibodies and uses thereof |
| AU2022335002A1 (en) * | 2021-08-27 | 2024-04-11 | Janssen Biotech, Inc. | Anti-psma radioconjugates and uses thereof |
| CN118234750A (zh) * | 2021-09-02 | 2024-06-21 | 纪念斯隆-凯特琳癌症中心 | 抗cd33抗体和其用途 |
| AU2022340644A1 (en) * | 2021-09-02 | 2024-02-29 | Memorial Hospital For Cancer And Allied Diseases | Antigen recognizing receptors targeting cd33 and uses thereof |
| WO2023034950A2 (en) * | 2021-09-03 | 2023-03-09 | Icahn School Of Medicine At Mount Sinai | Anti-hcmv antibodies and antigen-binding fragments thereof |
| EP4466284A2 (en) * | 2021-10-27 | 2024-11-27 | Twist Bioscience Corporation | Multispecific sars-cov-2 antibodies and methods of use |
| CN116333123A (zh) * | 2021-12-22 | 2023-06-27 | 宝船生物医药科技(上海)有限公司 | 抗tnfr2抗体及其用途 |
| EP4486382A2 (en) * | 2022-03-04 | 2025-01-08 | Coagulant Therapeutics Corporation | Human antibodies against activated protein c and uses thereof |
| JP2025534161A (ja) * | 2022-10-04 | 2025-10-14 | ミンク セラピューティクス, インコーポレイテッド | 線維芽細胞活性化タンパク質(fap)carインバリアントナチュラルキラーt細胞及びその使用 |
| AU2024240129A1 (en) | 2023-03-17 | 2025-10-09 | Oxitope Pharma B.V. | Anti-phosphocholine antibodies and methods of use thereof |
| CN116514998B (zh) * | 2023-05-12 | 2023-09-15 | 再少年(北京)生物科技有限公司 | 一种嵌合抗原受体、嵌合抗原受体-自然杀伤细胞及其在制备抗肿瘤药物中的应用 |
| WO2025010375A2 (en) * | 2023-07-03 | 2025-01-09 | Uct Bioscience Co., Ltd. | Anti-grp78 antibodies and their uses |
| WO2025015320A2 (en) * | 2023-07-12 | 2025-01-16 | Phenomic Ai | Anti-cthrc1 t cell engagers and methods of using the same |
| TW202525855A (zh) * | 2023-08-31 | 2025-07-01 | 美商艾克塞里克斯公司 | Ilt2結合劑及其用途 |
| TW202515919A (zh) * | 2023-09-01 | 2025-04-16 | 比利時商艾托斯比利時公司 | 抗trem2抗體及使用方法 |
| WO2025081163A1 (en) * | 2023-10-13 | 2025-04-17 | GenCART, Inc. | Compositions for the treatment of cancer |
| US20250255980A1 (en) * | 2024-02-12 | 2025-08-14 | Mbrace Therapeutics, Inc. | Antibody-Drug Conjugates Against the Glucose-regulating Protein 78 (GRP78) |
| WO2025184137A1 (en) * | 2024-02-27 | 2025-09-04 | Adimab, Llc | Influenza antibodies and methods of use thereof |
| CN117986346B (zh) * | 2024-04-07 | 2024-07-26 | 中国人民解放军军事科学院军事医学研究院 | 一种tpo模拟肽及其应用 |
| CN120399078A (zh) * | 2025-05-08 | 2025-08-01 | 上海天泽云泰生物医药有限公司 | 靶向cd117的纳米抗体及其应用 |
Family Cites Families (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751180A (en) | 1985-03-28 | 1988-06-14 | Chiron Corporation | Expression using fused genes providing for protein product |
| US4935233A (en) | 1985-12-02 | 1990-06-19 | G. D. Searle And Company | Covalently linked polypeptide cell modulators |
| DE68913658T3 (de) | 1988-11-11 | 2005-07-21 | Stratagene, La Jolla | Klonierung von Immunglobulin Sequenzen aus den variablen Domänen |
| JP3127158B2 (ja) | 1989-10-05 | 2001-01-22 | オプテイン,インコーポレイティド | 新規の遺伝子及びポリペチドの無細胞合成並びに単離 |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| CA2095633C (en) | 1990-12-03 | 2003-02-04 | Lisa J. Garrard | Enrichment method for variant proteins with altered binding properties |
| US20030036092A1 (en) | 1991-11-15 | 2003-02-20 | Board Of Regents, The University Of Texas System | Directed evolution of enzymes and antibodies |
| US5866344A (en) | 1991-11-15 | 1999-02-02 | Board Of Regents, The University Of Texas System | Antibody selection methods using cell surface expressed libraries |
| DK1024191T3 (da) | 1991-12-02 | 2008-12-08 | Medical Res Council | Fremstilling af autoantistoffer fremvist på fag-overflader ud fra antistofsegmentbiblioteker |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| DK1143006T3 (da) | 1995-08-18 | 2008-07-14 | Morphosys Ip Gmbh | Vektorer/DNA-sekvenser fra humane kombinatoriske antistofbiblioteker |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| GB9703369D0 (en) | 1997-02-18 | 1997-04-09 | Lindqvist Bjorn H | Process |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| US20060122373A1 (en) | 1997-04-04 | 2006-06-08 | Millennium Pharmaceuticals, Inc. | Delta3, FTHMA-070, Tango85, Tango77, SPOIL,NEOKINE, Tango129 and integrin alpha subunit protein and nucleic acid molecules and uses thereof |
| AU6952498A (en) | 1997-04-04 | 1998-10-30 | Millenium Pharmaceuticals, Inc. | Novel human delta3 compositions and therapeutic and diagnostic uses therefor |
| DK1004669T3 (da) | 1997-05-14 | 2007-08-27 | Asahi Chemical Ind | Hidtil ukendte differentieringsinhibitor |
| CA2337492A1 (en) | 1998-07-27 | 2000-02-10 | Amgen Inc. | Delta-related polypeptides |
| EP1105427A2 (en) | 1998-08-17 | 2001-06-13 | Abgenix, Inc. | Generation of modified molecules with increased serum half-lives |
| US20020102613A1 (en) | 1999-05-18 | 2002-08-01 | Hoogenboom Hendricus Renerus Jacobus Mattheus | Novel Fab fragment libraries and methods for their use |
| GB0008419D0 (en) | 2000-04-05 | 2000-05-24 | Bioinvent Int Ab | A method for invitro molecular evolution of antibody function |
| WO2001097850A2 (en) | 2000-06-23 | 2001-12-27 | Schering Aktiengesellschaft | Combinations and compositions which interfere with vegf/vegf and angiopoietin/tie receptor function and their use (ii) |
| FR2816319B1 (fr) | 2000-11-08 | 2004-09-03 | Millegen | Utilisation d'adn polymerase mutagene pour la creation de mutations aleatoires |
| US20040253242A1 (en) * | 2000-12-05 | 2004-12-16 | Bowdish Katherine S. | Rationally designed antibodies |
| US7396917B2 (en) | 2000-12-05 | 2008-07-08 | Alexion Pharmaceuticals, Inc. | Rationally designed antibodies |
| US20030022240A1 (en) | 2001-04-17 | 2003-01-30 | Peizhi Luo | Generation and affinity maturation of antibody library in silico |
| EP1438402A2 (en) | 2001-08-22 | 2004-07-21 | Galapagos Genomics N.V. | Modulators of bone homeostasis identified in a high-throughput screen |
| WO2003035842A2 (en) | 2001-10-24 | 2003-05-01 | Dyax Corporation | Hybridization control of sequence variation |
| CA2485732A1 (en) * | 2002-05-20 | 2003-12-04 | Abmaxis, Inc. | Generation and selection of protein library in silico |
| EP1513879B1 (en) | 2002-06-03 | 2018-08-22 | Genentech, Inc. | Synthetic antibody phage libraries |
| US20050079574A1 (en) | 2003-01-16 | 2005-04-14 | Genentech, Inc. | Synthetic antibody phage libraries |
| US20120202710A1 (en) * | 2003-09-09 | 2012-08-09 | Integrigen, Inc. | Methods and compositions for generation of germline human antibody genes |
| WO2005092925A2 (en) | 2004-03-24 | 2005-10-06 | Xencor, Inc. | Immunoglobulin variants outside the fc region |
| US8048418B2 (en) | 2004-10-29 | 2011-11-01 | Regeneron Pharmaceuticals, Inc. | Therapeutic methods for inhibiting tumor growth with combination of Dll4 antagonists and VEGF antagonists |
| EP1848672A4 (en) | 2005-02-01 | 2009-11-04 | Morphosys Ag | LIBRARIES AND METHOD FOR ISOLATING ANTIBODIES |
| US8716195B2 (en) | 2005-11-14 | 2014-05-06 | Bioren, Inc. | Antibody ultrahumanization by predicted mature CDR blasting and cohort library generation and screening |
| CA2630839C (en) | 2005-12-16 | 2017-01-17 | Regeneron Pharmaceuticals, Inc. | Therapeutic methods for inhibiting tumor growth with dll4 antagonists |
| WO2007137616A1 (en) | 2006-05-30 | 2007-12-06 | Millegen | Highly diversified antibody libraries |
| CL2007001623A1 (es) | 2006-06-06 | 2008-01-18 | Genentech Inc | Anticuerpo anti-dll4; polinucleotido que lo codifica; vector y celula huesped que comprenden dicho polinucleotido; metodo para elaborar el anticuerpo e inmunojugado; metodo de deteccion de dll4 y metodo diagnostico de un trastorno asociado a dll4; composicion que comprende al anticuerpo. |
| JP2009539870A (ja) * | 2006-06-06 | 2009-11-19 | ジェネンテック・インコーポレーテッド | 血管発生の調節ための組成物および方法 |
| CN101500605B (zh) | 2006-08-07 | 2014-04-30 | 瑞泽恩制药公司 | Dll4拮抗剂在治疗局部缺血性损伤中的用途 |
| HUE028379T2 (en) * | 2006-09-29 | 2016-12-28 | Oncomed Pharm Inc | Preparations and procedures for the diagnosis and treatment of cancer |
| US20080100597A1 (en) | 2006-10-25 | 2008-05-01 | Macrovision Corporation | Method and apparatus to improve playability in overscan areas of a TV display |
| RU2448979C2 (ru) | 2006-12-14 | 2012-04-27 | Ридженерон Фармасьютикалз, Инк. | Антитела человека к дельта-подобному лиганду-4 человека |
| JP2010517944A (ja) | 2007-01-26 | 2010-05-27 | バイオインヴェント インターナショナル アーベー | Dll4シグナリング阻害薬およびその使用 |
| GB0709333D0 (en) | 2007-05-15 | 2007-06-20 | Smart Targeting Ltd | Binding protein |
| JP5882058B2 (ja) | 2008-11-07 | 2016-03-09 | ファブラス エルエルシー | 組合せ抗体ライブラリー及びその使用 |
-
2009
- 2009-11-04 JP JP2011535649A patent/JP5882058B2/ja not_active Expired - Fee Related
- 2009-11-04 JP JP2011535651A patent/JP2012508017A/ja active Pending
- 2009-11-04 WO PCT/US2009/063303 patent/WO2010054010A1/en not_active Ceased
- 2009-11-04 CA CA2742968A patent/CA2742968C/en not_active Expired - Fee Related
- 2009-11-04 AU AU2009313560A patent/AU2009313560B2/en not_active Ceased
- 2009-11-04 US US13/128,219 patent/US9221902B2/en active Active
- 2009-11-04 DK DK09748671.6T patent/DK2356270T3/da active
- 2009-11-04 AU AU2009313551A patent/AU2009313551B2/en not_active Ceased
- 2009-11-04 WO PCT/US2009/063299 patent/WO2010054007A1/en not_active Ceased
- 2009-11-04 US US13/128,236 patent/US9403904B2/en not_active Expired - Fee Related
- 2009-11-04 EP EP09748671.6A patent/EP2356270B1/en active Active
- 2009-11-04 EP EP09748672A patent/EP2356146A1/en not_active Withdrawn
- 2009-11-04 CA CA2742969A patent/CA2742969A1/en not_active Abandoned
-
2015
- 2015-08-10 JP JP2015157842A patent/JP2016040246A/ja active Pending
- 2015-12-04 US US14/959,940 patent/US10774138B2/en active Active
-
2016
- 2016-02-03 JP JP2016018531A patent/JP2016127850A/ja active Pending
- 2016-07-07 AU AU2016204719A patent/AU2016204719A1/en not_active Abandoned
-
2018
- 2018-03-06 AU AU2018201622A patent/AU2018201622A1/en not_active Abandoned
-
2020
- 2020-08-25 US US17/002,749 patent/US20200399359A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2742969A1 (en) | 2010-05-14 |
| CA2742968A1 (en) | 2010-05-14 |
| AU2009313551A1 (en) | 2010-05-14 |
| US20160194627A1 (en) | 2016-07-07 |
| US20200399359A1 (en) | 2020-12-24 |
| JP2016040246A (ja) | 2016-03-24 |
| WO2010054010A1 (en) | 2010-05-14 |
| US20120058906A1 (en) | 2012-03-08 |
| WO2010054007A1 (en) | 2010-05-14 |
| JP2016127850A (ja) | 2016-07-14 |
| US10774138B2 (en) | 2020-09-15 |
| AU2016204719A1 (en) | 2016-07-28 |
| EP2356146A1 (en) | 2011-08-17 |
| US9403904B2 (en) | 2016-08-02 |
| US20110318339A1 (en) | 2011-12-29 |
| US9221902B2 (en) | 2015-12-29 |
| JP2012508017A (ja) | 2012-04-05 |
| DK2356270T3 (da) | 2016-12-12 |
| EP2356270B1 (en) | 2016-08-24 |
| EP2356270A1 (en) | 2011-08-17 |
| AU2009313560A1 (en) | 2010-05-14 |
| AU2018201622A1 (en) | 2018-03-29 |
| AU2009313551B2 (en) | 2015-12-17 |
| AU2009313560B2 (en) | 2016-04-14 |
| JP2012508016A (ja) | 2012-04-05 |
| CA2742968C (en) | 2020-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5882058B2 (ja) | 組合せ抗体ライブラリー及びその使用 | |
| JP6007420B2 (ja) | 親和性成熟に基づく抗体最適化方法 | |
| US9644021B2 (en) | Bovine fusion antibodies | |
| CN105814074B (zh) | 具有超长互补决定区的人源化抗体 | |
| JP2018095645A (ja) | ヒト化抗体 | |
| CN104004094A (zh) | 使用通用抗体构架进行的兔抗体的人源化 | |
| CN104520321A (zh) | 超长互补决定区及其用途 | |
| JP6903083B2 (ja) | 汎用性抗体フレームワークを用いたイエウサギ抗体のヒト化 | |
| Klagge | Generation and Screening of the Synthetic Human Combinatorial Antibody Library HuCAL GOLD |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20121105 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20121105 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130111 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140422 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20140714 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20140722 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150417 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150703 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151019 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20151203 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20160104 |
|
| R155 | Notification before disposition of declining of application |
Free format text: JAPANESE INTERMEDIATE CODE: R155 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20160203 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5882058 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313113 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| S533 | Written request for registration of change of name |
Free format text: JAPANESE INTERMEDIATE CODE: R313533 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |