JP5879254B2 - イムノグロブリンa分泌促進剤 - Google Patents
イムノグロブリンa分泌促進剤 Download PDFInfo
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- JP5879254B2 JP5879254B2 JP2012503020A JP2012503020A JP5879254B2 JP 5879254 B2 JP5879254 B2 JP 5879254B2 JP 2012503020 A JP2012503020 A JP 2012503020A JP 2012503020 A JP2012503020 A JP 2012503020A JP 5879254 B2 JP5879254 B2 JP 5879254B2
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- iga
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- iga antibody
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- 210000000813 small intestine Anatomy 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/48—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/30—Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/32—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds
- A23G1/48—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G4/00—Chewing gum
- A23G4/06—Chewing gum characterised by the composition containing organic or inorganic compounds
- A23G4/068—Chewing gum characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/42—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/66—Papaveraceae (Poppy family), e.g. bloodroot
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Natural Medicines & Medicinal Plants (AREA)
- Veterinary Medicine (AREA)
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- Pharmacology & Pharmacy (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
ヒナゲシ花100gを粉砕した後、試料の重量に対して10倍量の水を加えて70℃で2時間抽出した。不溶物を濾過した後、抽出液を凍結乾燥して熱水抽出物37.5gを得た。
マウス(BALB/c系、雌、6週齢)の小腸を摘出し、パイエル板を採取して2%ウシ胎児血清(FCS)を含むRPMI1620培地に浸した。ハサミでパイエル板を裁断し、セルストレーナー(BD Biosciences製、孔径70μm)でリンパ球細胞を回収した。回収した細胞懸濁液を遠心分離し、上清を吸引除去した後、complete mediumを加えてリンパ球細胞懸濁液の細胞濃度を4×106 cells/mlに調整した。complete mediumには10% FCS、50μM 2−メルカプトエタノール、1mMピルビン酸ナトリウム、100U/mlペニシリン、100μg/mlストレプトマイシンを含むRPMI1620培地を用いた。96穴プレートの各ウェルにリンパ球細胞懸濁液と試料を溶解した培養培地(試料は終濃度で100ppmとなるように調整)を0.05mlずつ加え、37℃、5%CO2の条件で1週間培養した。培養液を回収して遠心分離した後、上清中の総IgA抗体量をサンドイッチELISA法により測定した。試料を添加せずに培養した対照の総IgA抗体量を1とし、試料を添加して培養した場合の総IgA抗体量を相対値として算出した。
上記サンドイッチELISA法による測定は以下の手順により行った。
普通飼料(MF, オリエンタル酵母)にヒナゲシ(花)熱水抽出物を5%(w/w)混合した試験飼料を調製した。7週齢のマウス(BALB/c系)6匹を対照群と試験群(各群3匹)に分け、対照群には普通飼料、試験群には試験飼料を自由摂取させて6週間飼育した。飼育開始から1週間毎に糞便を回収し、それぞれ100mg/mlの濃度となるようにリン酸緩衝液に懸濁させ、遠心分離した後、上清中の総IgA抗体量を実施例2と同様にサンドイッチELISA法で測定した。
普通飼料(MF, オリエンタル酵母)にヒナゲシ(花)熱水抽出物を5%(w/w)混合した試験飼料を調製した。7週齢のマウス(BALB/c系)12匹を対照群と試験群(各群6匹)に分け、対照群には普通飼料、試験群には試験飼料を自由摂取させて飼育した。4週目に50μgのホスホリルコリン−keyhole limpet hemocyanin(PC−KLH、細菌に共通の抗原)を経鼻投与して免疫した。その後も1週間毎に経鼻免疫し、3回目の経鼻免疫から1週間後に鼻腔粘液(100〜200μl/匹)及び鼻腔粘膜固有層リンパ球細胞(1×105〜5×105cells/匹)を回収し、総IgA抗体量及び総IgA抗体産生細胞数、PC特異的IgA抗体価及びPC特異的IgA抗体産生細胞数を測定した。また、糞便を回収し、100mg/mlの濃度となるようにリン酸緩衝液に懸濁させ、遠心分離した後、上清中の総IgA抗体量を測定した。総IgA抗体量及びPC特異的IgA抗体価の測定はサンドイッチELISA法で、PC特異的IgA抗体産生細胞数の測定はenzyme−linked immunospot(ELISPOT)法で行った。尚、各動物から採取できる鼻腔粘膜固有層リンパ球細胞の数は非常に少ないため、3匹分の細胞を一つにまとめて2連でIgA抗体産生細胞数の測定を行った。
上記総IgA抗体量の測定は実施例2と同様にサンドイッチELISA法により行った。また、上記PC特異的IgA抗体量の測定は、最初にELISAプレートに吸着させる抗マウスイムノグロブリン抗体の代わりにPC−BSAを用いたほかは実施例2と同様にしてサンドイッチELISA法により行った。
上記ELISPOT法によるPC特異的IgA抗体産生細胞数測定は以下の手順により行った。
CO2の条件下で4時間インキュベートした。PBSで洗浄後、100ngのペルオキシダーゼ結合抗マウスIgA抗体を加え、4℃で一晩反応させた。PBSで洗浄後、発色基質(3−amino−9−ethylcarbazole)液を加えて室温、遮光条件で30分発色させた。水で洗浄し乾燥させた後に、spotを抗体産生細胞として実体顕微鏡下にて計測した。
糞便を回収し、100mg/mlの濃度となるようにPBSに懸濁させ、遠心分離した後、上清の総IgA量を実施例2と同様にサンドイッチELISA法で測定した。
普通飼料(MF, オリエンタル酵母)にヒナゲシ(花)熱水抽出物を5%(w/w)混合した試験飼料を調製した。7週齢のマウス(BALB/c系)20匹を対照群と試験群(各群10匹)に分け、実施例4と同様の方法で鼻腔粘液の総IgA抗体量及びPC特異的IgA抗体価、糞便の総IgA抗体量を測定した。
7週齢のマウス(BALB/c系)を1群4匹として対照群と投与群に分け、普通飼料(MF, オリエンタル酵母)で飼育した。投与群には飼育開始時からヒナゲシ(花)熱水抽出物を4mg/dayの用量で水溶液として毎日舌下投与し、対照群は同量の水のみを舌下投与した。4週目に50μgのPC−KLHを経鼻投与して免疫し、その後も1週間毎に経鼻免疫して、3回目の経鼻免疫から1週間後に鼻腔粘液及び鼻腔粘膜固有層リンパ球細胞を回収し、総IgA抗体量及び総IgA抗体産生細胞数を測定した。総IgA抗体量の測定は実施例2と同様にサンドイッチELISA法で、IgA抗体産生細胞数の測定は実施例4と同様にenzyme−linked immunospot(ELISPOT)法で行った。尚、各動物から採取できる鼻腔粘膜固有層リンパ球細胞の数は非常に少ないので、4匹分の細胞をまとめて、2連でIgA抗体産生細胞数の測定を行った。
7週齢のマウス(BALB/c系)を1群15匹として対照群と投与群に分け、普通飼料(MF, オリエンタル酵母)で飼育した。投与群には飼育開始時からヒナゲシ(花)熱水抽出物を4mg/dayの用量で水溶液として毎日舌下投与し、対照群は同量の水のみを舌下投与した。4週目に50μgのPC−KLHを経鼻投与して免疫し、その後も1週間毎に経鼻免疫して、3回目の経鼻免疫から1週間後に鼻腔粘液及び鼻腔粘膜固有層リンパ球細胞を回収し、総IgA抗体量及び総IgA抗体産生細胞数、PC特異的IgA抗体価及びPC特異的IgA抗体産生細胞数を測定した。また、糞便を回収してリン酸緩衝液に懸濁させ、遠心分離した後、上清中の総IgA抗体量を測定した。総IgA抗体量及びPC特異的IgA抗体価の測定は実施例2と同様にサンドイッチELISA法で、IgA抗体産生細胞数の測定は実施例4と同様にenzyme−linked immunospot(ELISPOT)法で行った。尚、各動物から採取できる鼻腔粘膜固有層リンパ球細胞の数は非常に少ないので、3匹分の細胞をまとめて、2連でIgA抗体産生細胞数の測定を行った。
7週齢のマウス(BALB/c系)を1群6匹として対照群と投与群に分け、普通飼料(MF, オリエンタル酵母)で飼育した。投与群には飼育開始時からヒナゲシ(花)熱水抽出物を2mg/dayの用量で水溶液として毎日経鼻投与し、対照群は同量の水のみを経鼻投与した。4週目に50μgのPC−KLHを経鼻投与して免疫し、その後も1週間毎に経鼻免疫して、3回目の経鼻免疫から1週間後に鼻腔粘液及び鼻腔粘膜固有層リンパ球細胞を回収し、総IgA抗体量及び総IgA抗体産生細胞数を測定した。総IgA抗体量の測定は実施例2と同様にサンドイッチELISA法で、IgA抗体産生細胞数の測定は実施例4と同様にenzyme−linked immunospot(ELISPOT)法で行った。尚、各動物から採取できる鼻腔粘膜固有層リンパ球細胞の数は非常に少ないので、3匹分の細胞をまとめて、2連でIgA抗体産生細胞数の測定を行った。
5〜6ヶ月齢のマウス(BALB/c系)を1群5匹として対照群と投与群に分け、普通飼料(MF, オリエンタル酵母)で飼育した。投与群には飼育開始時からヒナゲシ(花)熱水抽出物を2mg/dayの用量で水溶液として毎日経鼻投与し、対照群は同量の水のみを経鼻投与した。4週目に50μgのPC−KLHを経鼻投与して免疫し、その後も1週間毎に経鼻免疫して、3回目の経鼻免疫から1週間後に鼻腔粘液及び鼻腔粘膜固有層リンパ球細胞を回収し、総IgA抗体量及び総IgA抗体産生細胞数を測定した。総IgA抗体量の測定は実施例2と同様にサンドイッチELISA法で、IgA抗体産生細胞数の測定は実施例4と同様にenzyme−linked immunospot(ELISPOT)法で行った。尚、各動物から採取できる鼻腔粘膜固有層リンパ球細胞の数は非常に少ないので、2〜3匹分の細胞をまとめて、2連でIgA抗体産生細胞数の測定を行った。
D−マンニトール 35.0%
乳糖 27.5
結晶セルロース 12.5
ヒドロキシプロピルセルロース 5.0
実施例1のヒナゲシ抽出物 20.0
100.0%
乳糖 54.5%
トウモロコシデンプン 38.5
ステアリン酸マグネシウム 2.0
実施例1のヒナゲシ抽出物 5.0
100.0%
乳糖 8.0%
ステアリン酸マグネシウム 2.0
実施例1のヒナゲシ抽出物 90.0
100.0%
乳糖 40.0%
馬鈴薯でんぷん 15.0
実施例1のヒナゲシ抽出物 45.0
100.0%
エタノール 5.0%
実施例1のヒナゲシ抽出物 0.5
L−メントール 1.0
水 93.5
100.0%
サリチル酸メチル 0.03g
マレイン酸クロルフェニラミン 0.3
dl―塩酸メチルエフェドリン 0.3
ポリソルベート80 0.2
塩化ベンザルコニウム 0.01
塩化ナトリウム 0.6
1N水酸化ナトリウム 適量
実施例1のヒナゲシ抽出物 0.01
水 適量
100.0ml
(pH6.8)
ガムベース 20.0%
砂糖 52.0
グルコース 14.0
水飴 13.0
香料 0.5
実施例1のヒナゲシ抽出物 0.5
100.0%
ガムベース 20.0%
砂糖 53.0
グルコース 15.0
水飴 10.5
香料 0.5
実施例1のヒナゲシ抽出物 1.0
100.0%
ガムベース 20.0%
砂糖 54.0
グルコース 14.0
水飴 9.3
香料 0.7
実施例1のヒナゲシ抽出物 2.0
100.0%
砂糖 45.0%
水飴 40.0
香料 0.5
L−メントール 0.3
実施例1のヒナゲシ抽出物 0.2
水 14.0
100.0%
砂糖 50.0%
水飴 33.0
クエン酸 1.0
香料 0.6
実施例1のヒナゲシ抽出物 1.0
水 14.4
100.0%
カカオビター 20.0%
全脂粉乳 20.0
カカオバター 17.0
粉糖 41.7
レシチン 0.5
香料 0.2
実施例1のヒナゲシ抽出物 0.6
100.0%
砂糖 31.0%
小麦粉 26.5
片栗粉 26.5
バター 4.0
卵 10.5
重曹 0.3
実施例1のヒナゲシ抽出物 1.2
100.0%
ポリデキストロース水溶液 38.0%
ソルビトール水溶液 8.0
パラチノース水溶液 9.0
マルトース水溶液 20.0
トレハロース水溶液 11.0
ゼラチン 10.0
酒石酸 1.0
実施例1のヒナゲシ抽出物 3.0
100.0%
砂糖 76.0%
グルコース 20.0
ショ糖脂肪酸エステル 0.2
香料 0.1
実施例1のヒナゲシ抽出物 0.5
水 3.2
100.0%
砂糖 72.0%
乳糖 19.8
ショ糖脂肪酸エステル 0.2
実施例1のヒナゲシ抽出物 5.0
水 3.0
100.0%
砂糖 28.8%
ショ糖脂肪酸エステル 0.2
実施例1のヒナゲシ抽出物 70.0
水 1.0
100.0%
卵黄 11.0%
砂糖 13.5
牛乳 37.0
生クリーム 37.0
バニラビーンズ 0.5
実施例1のヒナゲシ抽出物 1.0
100.0%
オレンジ果汁 20.0%
砂糖 29.5
実施例1のヒナゲシ抽出物 0.5
水 50.0
100.0%
オレンジ果汁 40.0%
異性化糖 15.3
クエン酸 0.1
ビタミンC 0.05
香料 0.1
実施例1のヒナゲシ抽出物 0.05
水 44.4
100.0%
グレープ果汁 70.0%
異性化糖 12.0
クエン酸 0.1
ビタミンC 0.05
香料 0.1
実施例1のヒナゲシ抽出物 0.1
水 適量
100.0%
Claims (9)
- ヒナゲシの花を、70℃の抽出温度にて、0.5〜5時間の抽出時間で、水で抽出した抽出物を、抽出後に濾過し、凍結乾燥工程を経ることにより得られた抽出物を含有し、錠剤、カプセル剤、散剤、シロップ剤、ドリンク剤から選択されるいずれかの形態であることを特徴とする、IgA分泌促進剤の製造方法。
- 前記抽出時間が2時間であることを特徴とする、請求項1に記載のIgA分泌促進剤の製造方法。
- 請求項1または2に記載のIgA分泌促進剤を乾燥重量に換算して0.01%〜90%含有することを特徴とする医薬組成物の製造方法。
- 経口摂取されることを特徴とする請求項3に記載の医薬組成物の製造方法。
- 舌下投与により摂取されることを特徴とする請求項3に記載の医薬組成物の製造方法。
- 経鼻投与により摂取されることを特徴とする請求項3に記載の医薬組成物の製造方法。
- 請求項1または2に記載のIgA分泌促進剤を含有することを特徴とする医薬組成物の製造方法。
- IgA分泌促進効果を有する、70℃の抽出温度にて、0.5〜5時間の抽出時間で、水を用いて抽出し、抽出後に濾過し、凍結乾燥工程を経ることにより得られる、ヒナゲシの花の抽出物の製造方法。
- 前記抽出時間が2時間であることを特徴とする、請求項8に記載の抽出物の製造方法。
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JP (1) | JP5879254B2 (ja) |
KR (1) | KR101896123B1 (ja) |
CN (1) | CN102781457B (ja) |
BR (1) | BR112012022203A2 (ja) |
HK (1) | HK1176546A1 (ja) |
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JP7313519B1 (ja) * | 2022-07-08 | 2023-07-24 | 日本甜菜製糖株式会社 | IgA産生促進剤及びIgA産生促進用の飼料組成物 |
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Also Published As
Publication number | Publication date |
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EP2543381A1 (en) | 2013-01-09 |
TW201141499A (en) | 2011-12-01 |
KR101896123B1 (ko) | 2018-09-07 |
WO2011108275A1 (ja) | 2011-09-09 |
HK1176546A1 (en) | 2013-08-02 |
BR112012022203A2 (pt) | 2016-07-05 |
CN102781457B (zh) | 2015-01-14 |
KR20130037672A (ko) | 2013-04-16 |
JPWO2011108275A1 (ja) | 2013-06-20 |
EP2543381A4 (en) | 2013-09-18 |
CN102781457A (zh) | 2012-11-14 |
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