JP5808742B2 - 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 - Google Patents

連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 Download PDF

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JP5808742B2
JP5808742B2 JP2012522190A JP2012522190A JP5808742B2 JP 5808742 B2 JP5808742 B2 JP 5808742B2 JP 2012522190 A JP2012522190 A JP 2012522190A JP 2012522190 A JP2012522190 A JP 2012522190A JP 5808742 B2 JP5808742 B2 JP 5808742B2
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cell
cells
culture
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cell culture
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JP2013500710A (ja
Inventor
レオポルト グリルベルガー，
レオポルト グリルベルガー，
マンフレート ライター，
マンフレート ライター，
ダニエル フライシャンデルル，
ダニエル フライシャンデルル，
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Baxter Healthcare SA
Baxter International Inc
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Baxter Healthcare SA
Baxter International Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/006—Cell injection or fusion devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/00051—Methods of production or purification of viral material
    • C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2012522190A 2009-07-31 2010-08-02 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 Active JP5808742B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23031309P 2009-07-31 2009-07-31
US61/230,313 2009-07-31
PCT/EP2010/061191 WO2011012725A1 (en) 2009-07-31 2010-08-02 Method of producing a polypeptide or virus of interest in a continuous cell culture

Related Child Applications (1)

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JP2015004718A Division JP6177260B2 (ja) 2009-07-31 2015-01-14 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法

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JP2013500710A JP2013500710A (ja) 2013-01-10
JP5808742B2 true JP5808742B2 (ja) 2015-11-10

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JP2012522190A Active JP5808742B2 (ja) 2009-07-31 2010-08-02 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法
JP2015004718A Active JP6177260B2 (ja) 2009-07-31 2015-01-14 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法

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US (4) US8580554B2 (enExample)
EP (2) EP3480292A1 (enExample)
JP (2) JP5808742B2 (enExample)
KR (2) KR101885046B1 (enExample)
CN (1) CN102549142B (enExample)
AU (1) AU2010277490B2 (enExample)
BR (1) BR112012002137B8 (enExample)
CA (1) CA2769354A1 (enExample)
CO (1) CO6491119A2 (enExample)
DK (1) DK2459697T3 (enExample)
EA (1) EA022219B1 (enExample)
ES (1) ES2705206T3 (enExample)
HR (1) HRP20182165T1 (enExample)
IN (1) IN2012DN00899A (enExample)
MX (2) MX2012001274A (enExample)
NZ (1) NZ597742A (enExample)
PL (1) PL2459697T3 (enExample)
PT (1) PT2459697T (enExample)
SG (1) SG178176A1 (enExample)
SI (1) SI2459697T1 (enExample)
TR (1) TR201900213T4 (enExample)
WO (1) WO2011012725A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE11738351T2 (hu) 2010-07-08 2019-03-28 Baxalta GmbH Eljárás rekombináns ADAMTS13 elõállítására sejttenyészetben
SI2665806T1 (sl) * 2011-01-17 2021-11-30 F. Hoffmann-La Roche Ag Aparat za separacijo
US9115381B2 (en) 2011-05-13 2015-08-25 Octapharma Ag Method of increasing the productivity of eucaryotic cells in the production of recombinant FVIII
KR20230114317A (ko) 2011-07-01 2023-08-01 암젠 인크 포유동물 세포 배양
CN203129697U (zh) 2013-02-05 2013-08-14 客贝利(厦门)休闲用品有限公司 一种帐篷架杆
JP6280869B2 (ja) 2011-11-24 2018-02-14 ジェネトン 工業的な医薬用途に適合する拡張可能なレンチウイルスベクター製造システム
US9574167B2 (en) 2013-02-15 2017-02-21 Pharyx, Inc. Methods and apparatus for independent control of product and reactant concentrations
EP2964663A2 (en) 2013-03-08 2016-01-13 Genzyme Corporation Continuous purification of therapeutic proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
EP2976418B1 (en) 2013-03-19 2020-09-30 CMC Biologics A/S A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process.
ES2778928T3 (es) * 2013-08-30 2020-08-12 Glaxosmithkline Biologicals Sa Producción a gran escala de virus en cultivos celulares
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
WO2015149143A2 (en) 2014-04-01 2015-10-08 Advantech Bioscience Farmacêutica Ltda. Stable factor viii formulations with low sugar-glycine
CN106456720A (zh) 2014-04-01 2017-02-22 爱德技术生物科学有限公司 在无钙作为赋形剂下因子viii的稳定性
EP3158055B1 (en) * 2014-06-13 2019-09-11 CSL Limited Improved production of recombinant von willebrand factor in a bioreactor
CN116083345A (zh) * 2016-01-26 2023-05-09 勃林格殷格翰国际公司 对连续流动搅拌釜反应器细胞培养系统的连接灌注
KR102414290B1 (ko) 2016-04-15 2022-06-29 베링거 인겔하임 인터내셔날 게엠베하 세포 보유 장치 및 방법
HRP20210163T1 (hr) * 2016-12-21 2021-03-19 F. Hoffmann - La Roche Ag Kontrola rasta eukariotskih stanica
IL321253A (en) 2018-05-01 2025-08-01 Amgen Inc Antibodies with a modulated glycan profile
WO2019239780A1 (ja) * 2018-06-15 2019-12-19 富士フイルム株式会社 細胞培養方法、細胞培養装置、及び生産物の製造方法
CA3110666A1 (en) 2018-08-31 2020-03-05 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
CN113355297A (zh) * 2021-06-23 2021-09-07 吉林冠界生物技术有限公司 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5019512A (en) 1989-03-17 1991-05-28 Baxter International Inc. Spin filter for removing substantially cell-free culture medium from suspension cell culture system
US5626734A (en) 1995-08-18 1997-05-06 University Technologies International, Inc. Filter for perfusion cultures of animal cells and the like
JP4361730B2 (ja) 2000-10-02 2009-11-11 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト ビタミンｋ−依存性タンパク質の生成方法
AU2002342922A1 (en) * 2001-11-28 2003-06-10 Sandoz Ag Cell culture process
US7294484B2 (en) 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
PL1902141T3 (pl) 2005-06-17 2012-12-31 Baxalta Inc Kompozycje o działaniu trombolitycznym zawierające ADAMTS13
CA2633306A1 (en) * 2006-01-04 2007-07-12 Baxter International Inc. Oligopeptide-free cell culture media
AU2008345231B2 (en) * 2007-12-27 2014-09-11 Takeda Pharmaceutical Company Limited Cell culture processes

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Publication number Publication date
EA022219B1 (ru) 2015-11-30
DK2459697T3 (en) 2019-01-28
US20190153400A1 (en) 2019-05-23
EP2459697B1 (en) 2018-10-17
EP2459697A1 (en) 2012-06-06
JP6177260B2 (ja) 2017-08-09
KR20170077286A (ko) 2017-07-05
MX2012001274A (es) 2012-06-01
US10221400B2 (en) 2019-03-05
BR112012002137B1 (pt) 2020-04-14
US10745672B2 (en) 2020-08-18
BR112012002137B8 (pt) 2020-05-12
CN102549142A (zh) 2012-07-04
MX353610B (es) 2018-01-18
KR101752789B1 (ko) 2017-06-30
EP3480292A1 (en) 2019-05-08
US20140038264A1 (en) 2014-02-06
US20180051261A1 (en) 2018-02-22
EA201270213A1 (ru) 2012-06-29
NZ597742A (en) 2014-02-28
PT2459697T (pt) 2019-01-21
JP2013500710A (ja) 2013-01-10
HRP20182165T1 (hr) 2019-03-08
US8580554B2 (en) 2013-11-12
SG178176A1 (en) 2012-03-29
CN102549142B (zh) 2014-07-16
CO6491119A2 (es) 2012-07-31
KR101885046B1 (ko) 2018-08-02
KR20120038531A (ko) 2012-04-23
ES2705206T3 (es) 2019-03-22
US20110086411A1 (en) 2011-04-14
IN2012DN00899A (enExample) 2015-04-03
WO2011012725A1 (en) 2011-02-03
CA2769354A1 (en) 2011-02-03
US9650612B2 (en) 2017-05-16
AU2010277490A1 (en) 2012-02-09
WO2011012725A8 (en) 2011-05-05
TR201900213T4 (tr) 2019-02-21
SI2459697T1 (sl) 2019-03-29
BR112012002137A2 (pt) 2015-09-15
PL2459697T3 (pl) 2019-04-30
JP2015062436A (ja) 2015-04-09
AU2010277490B2 (en) 2014-08-28

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