JP5808742B2 - 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 - Google Patents

連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 Download PDF

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JP5808742B2
JP5808742B2 JP2012522190A JP2012522190A JP5808742B2 JP 5808742 B2 JP5808742 B2 JP 5808742B2 JP 2012522190 A JP2012522190 A JP 2012522190A JP 2012522190 A JP2012522190 A JP 2012522190A JP 5808742 B2 JP5808742 B2 JP 5808742B2
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cell
cells
culture
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cell culture
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JP2013500710A (ja
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レオポルト グリルベルガー，
レオポルト グリルベルガー，
マンフレート ライター，
マンフレート ライター，
ダニエル フライシャンデルル，
ダニエル フライシャンデルル，
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Baxter Healthcare SA
Baxter International Inc
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Baxter International Inc
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/006—Cell injection or fusion devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/00051—Methods of production or purification of viral material
    • C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2012522190A 2009-07-31 2010-08-02 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法 Active JP5808742B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23031309P 2009-07-31 2009-07-31
US61/230,313 2009-07-31
PCT/EP2010/061191 WO2011012725A1 (en) 2009-07-31 2010-08-02 Method of producing a polypeptide or virus of interest in a continuous cell culture

Related Child Applications (1)

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JP2015004718A Division JP6177260B2 (ja) 2009-07-31 2015-01-14 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法

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JP2013500710A JP2013500710A (ja) 2013-01-10
JP5808742B2 true JP5808742B2 (ja) 2015-11-10

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JP2012522190A Active JP5808742B2 (ja) 2009-07-31 2010-08-02 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法
JP2015004718A Active JP6177260B2 (ja) 2009-07-31 2015-01-14 連続細胞培養で対象となるポリペプチドまたはウイルスを生成する方法

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US (4) US8580554B2 (enExample)
EP (2) EP3480292A1 (enExample)
JP (2) JP5808742B2 (enExample)
KR (2) KR101752789B1 (enExample)
CN (1) CN102549142B (enExample)
AU (1) AU2010277490B2 (enExample)
BR (1) BR112012002137B8 (enExample)
CA (1) CA2769354A1 (enExample)
CO (1) CO6491119A2 (enExample)
DK (1) DK2459697T3 (enExample)
EA (1) EA022219B1 (enExample)
ES (1) ES2705206T3 (enExample)
HR (1) HRP20182165T1 (enExample)
IN (1) IN2012DN00899A (enExample)
MX (2) MX2012001274A (enExample)
NZ (1) NZ597742A (enExample)
PL (1) PL2459697T3 (enExample)
PT (1) PT2459697T (enExample)
SG (1) SG178176A1 (enExample)
SI (1) SI2459697T1 (enExample)
TR (1) TR201900213T4 (enExample)
WO (1) WO2011012725A1 (enExample)

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PT3626736T (pt) 2010-07-08 2025-08-11 Takeda Pharmaceuticals Co Método de produção de fvw recombinante de elevado peso molecular na cultura celular
SG191745A1 (en) * 2011-01-17 2013-08-30 Hoffmann La Roche Separation apparatus and use thereof
CA2835053C (en) 2011-05-13 2020-07-07 Octapharma Ag A method of increasing the productivity of eucaryotic cells in the production of recombinant fviii
KR101662412B1 (ko) * 2011-07-01 2016-10-04 암젠 인크 포유동물 세포 배양
CN203129697U (zh) 2013-02-05 2013-08-14 客贝利(厦门)休闲用品有限公司 一种帐篷架杆
CN104136605B (zh) 2011-11-24 2018-08-14 吉尼松公司 与工业制药应用相容的可放大的慢病毒载体生产系统
US9574167B2 (en) 2013-02-15 2017-02-21 Pharyx, Inc. Methods and apparatus for independent control of product and reactant concentrations
CN118388581A (zh) 2013-03-08 2024-07-26 建新公司 治疗性蛋白药物物质的整合连续制造
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
ES2842350T3 (es) 2013-03-19 2021-07-13 Cmc Biologics As Un procedimiento para la producción de un producto (por ejemplo, polipéptido) en un proceso de fermentación de cultivo celular continuo
CA2920934C (en) * 2013-08-30 2022-12-06 Glaxosmithkline Biologicals S.A. Large scale production of viruses in cell culture
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
KR20160146763A (ko) 2014-04-01 2016-12-21 어드밴텍 바이오사이언스 파마큐티카 엘티디에이 부형제로서 칼슘이 첨가되지 않은 factor viii의 안정화
JP2017509659A (ja) 2014-04-01 2017-04-06 アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ 低糖−低グリシンの安定な第ｖｉｉｉ因子製剤
CN106414719B (zh) * 2014-06-13 2020-02-14 杰特有限公司 生物反应器中重组von Willebrand因子的改进生产
KR20240173213A (ko) * 2016-01-26 2024-12-10 베링거 인겔하임 인터내셔날 게엠베하 연결된 관류 대 연속-유동식 교반-탱크 반응기 세포 배양 시스템
EP3443064A1 (en) * 2016-04-15 2019-02-20 Boehringer Ingelheim International GmbH Cell retention device and method
US20200063086A1 (en) * 2016-12-21 2020-02-27 Hoffmann-La Roche Inc. Growth control of eukaryotic cells
IL321253A (en) 2018-05-01 2025-08-01 Amgen Inc Antibodies with a modulated glycan profile
WO2019239780A1 (ja) * 2018-06-15 2019-12-19 富士フイルム株式会社 細胞培養方法、細胞培養装置、及び生産物の製造方法
BR112021003420A2 (pt) 2018-08-31 2021-05-18 Genzyme Corporation resina cromatográfica estéril e uso da mesma em processos de manufatura
CN113355297A (zh) * 2021-06-23 2021-09-07 吉林冠界生物技术有限公司 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法

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CA2769354A1 (en) 2011-02-03
US20190153400A1 (en) 2019-05-23
IN2012DN00899A (enExample) 2015-04-03
KR20120038531A (ko) 2012-04-23
WO2011012725A1 (en) 2011-02-03
BR112012002137A2 (pt) 2015-09-15
NZ597742A (en) 2014-02-28
JP6177260B2 (ja) 2017-08-09
US9650612B2 (en) 2017-05-16
TR201900213T4 (tr) 2019-02-21
HRP20182165T1 (hr) 2019-03-08
SG178176A1 (en) 2012-03-29
US10745672B2 (en) 2020-08-18
CN102549142B (zh) 2014-07-16
EP2459697B1 (en) 2018-10-17
US20140038264A1 (en) 2014-02-06
BR112012002137B8 (pt) 2020-05-12
SI2459697T1 (sl) 2019-03-29
PT2459697T (pt) 2019-01-21
US10221400B2 (en) 2019-03-05
EP2459697A1 (en) 2012-06-06
EA022219B1 (ru) 2015-11-30
MX353610B (es) 2018-01-18
EA201270213A1 (ru) 2012-06-29
PL2459697T3 (pl) 2019-04-30
AU2010277490A1 (en) 2012-02-09
EP3480292A1 (en) 2019-05-08
US20180051261A1 (en) 2018-02-22
KR101752789B1 (ko) 2017-06-30
CN102549142A (zh) 2012-07-04
US20110086411A1 (en) 2011-04-14
BR112012002137B1 (pt) 2020-04-14
CO6491119A2 (es) 2012-07-31
ES2705206T3 (es) 2019-03-22
KR101885046B1 (ko) 2018-08-02
US8580554B2 (en) 2013-11-12
DK2459697T3 (en) 2019-01-28
AU2010277490B2 (en) 2014-08-28
WO2011012725A8 (en) 2011-05-05
KR20170077286A (ko) 2017-07-05
JP2013500710A (ja) 2013-01-10
MX2012001274A (es) 2012-06-01
JP2015062436A (ja) 2015-04-09

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