EA022219B1 - Способ получения полипептида или вируса, представляющих интерес, в непрерывной клеточной культуре - Google Patents
Способ получения полипептида или вируса, представляющих интерес, в непрерывной клеточной культуре Download PDFInfo
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- EA022219B1 EA022219B1 EA201270213A EA201270213A EA022219B1 EA 022219 B1 EA022219 B1 EA 022219B1 EA 201270213 A EA201270213 A EA 201270213A EA 201270213 A EA201270213 A EA 201270213A EA 022219 B1 EA022219 B1 EA 022219B1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C12N2710/00011—Details
- C12N2710/00051—Methods of production or purification of viral material
- C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23031309P | 2009-07-31 | 2009-07-31 | |
| PCT/EP2010/061191 WO2011012725A1 (en) | 2009-07-31 | 2010-08-02 | Method of producing a polypeptide or virus of interest in a continuous cell culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201270213A1 EA201270213A1 (ru) | 2012-06-29 |
| EA022219B1 true EA022219B1 (ru) | 2015-11-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201270213A EA022219B1 (ru) | 2009-07-31 | 2010-08-02 | Способ получения полипептида или вируса, представляющих интерес, в непрерывной клеточной культуре |
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| EP (2) | EP3480292A1 (enExample) |
| JP (2) | JP5808742B2 (enExample) |
| KR (2) | KR101885046B1 (enExample) |
| CN (1) | CN102549142B (enExample) |
| AU (1) | AU2010277490B2 (enExample) |
| BR (1) | BR112012002137B8 (enExample) |
| CA (1) | CA2769354A1 (enExample) |
| CO (1) | CO6491119A2 (enExample) |
| DK (1) | DK2459697T3 (enExample) |
| EA (1) | EA022219B1 (enExample) |
| ES (1) | ES2705206T3 (enExample) |
| HR (1) | HRP20182165T1 (enExample) |
| IN (1) | IN2012DN00899A (enExample) |
| MX (2) | MX2012001274A (enExample) |
| NZ (1) | NZ597742A (enExample) |
| PL (1) | PL2459697T3 (enExample) |
| PT (1) | PT2459697T (enExample) |
| SG (1) | SG178176A1 (enExample) |
| SI (1) | SI2459697T1 (enExample) |
| TR (1) | TR201900213T4 (enExample) |
| WO (1) | WO2011012725A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HUE11738351T2 (hu) | 2010-07-08 | 2019-03-28 | Baxalta GmbH | Eljárás rekombináns ADAMTS13 elõállítására sejttenyészetben |
| SI2665806T1 (sl) * | 2011-01-17 | 2021-11-30 | F. Hoffmann-La Roche Ag | Aparat za separacijo |
| US9115381B2 (en) | 2011-05-13 | 2015-08-25 | Octapharma Ag | Method of increasing the productivity of eucaryotic cells in the production of recombinant FVIII |
| KR20230114317A (ko) | 2011-07-01 | 2023-08-01 | 암젠 인크 | 포유동물 세포 배양 |
| CN203129697U (zh) | 2013-02-05 | 2013-08-14 | 客贝利(厦门)休闲用品有限公司 | 一种帐篷架杆 |
| JP6280869B2 (ja) | 2011-11-24 | 2018-02-14 | ジェネトン | 工業的な医薬用途に適合する拡張可能なレンチウイルスベクター製造システム |
| US9574167B2 (en) | 2013-02-15 | 2017-02-21 | Pharyx, Inc. | Methods and apparatus for independent control of product and reactant concentrations |
| EP2964663A2 (en) | 2013-03-08 | 2016-01-13 | Genzyme Corporation | Continuous purification of therapeutic proteins |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| EP2976418B1 (en) | 2013-03-19 | 2020-09-30 | CMC Biologics A/S | A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process. |
| ES2778928T3 (es) * | 2013-08-30 | 2020-08-12 | Glaxosmithkline Biologicals Sa | Producción a gran escala de virus en cultivos celulares |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| TWI671312B (zh) | 2014-01-17 | 2019-09-11 | 美商健臻公司 | 無菌層析法及製法 |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| WO2015149143A2 (en) | 2014-04-01 | 2015-10-08 | Advantech Bioscience Farmacêutica Ltda. | Stable factor viii formulations with low sugar-glycine |
| CN106456720A (zh) | 2014-04-01 | 2017-02-22 | 爱德技术生物科学有限公司 | 在无钙作为赋形剂下因子viii的稳定性 |
| EP3158055B1 (en) * | 2014-06-13 | 2019-09-11 | CSL Limited | Improved production of recombinant von willebrand factor in a bioreactor |
| CN116083345A (zh) * | 2016-01-26 | 2023-05-09 | 勃林格殷格翰国际公司 | 对连续流动搅拌釜反应器细胞培养系统的连接灌注 |
| KR102414290B1 (ko) | 2016-04-15 | 2022-06-29 | 베링거 인겔하임 인터내셔날 게엠베하 | 세포 보유 장치 및 방법 |
| HRP20210163T1 (hr) * | 2016-12-21 | 2021-03-19 | F. Hoffmann - La Roche Ag | Kontrola rasta eukariotskih stanica |
| IL321253A (en) | 2018-05-01 | 2025-08-01 | Amgen Inc | Antibodies with a modulated glycan profile |
| WO2019239780A1 (ja) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | 細胞培養方法、細胞培養装置、及び生産物の製造方法 |
| CA3110666A1 (en) | 2018-08-31 | 2020-03-05 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| CN113355297A (zh) * | 2021-06-23 | 2021-09-07 | 吉林冠界生物技术有限公司 | 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5019512A (en) | 1989-03-17 | 1991-05-28 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| US5626734A (en) | 1995-08-18 | 1997-05-06 | University Technologies International, Inc. | Filter for perfusion cultures of animal cells and the like |
| JP4361730B2 (ja) | 2000-10-02 | 2009-11-11 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | ビタミンｋ−依存性タンパク質の生成方法 |
| AU2002342922A1 (en) * | 2001-11-28 | 2003-06-10 | Sandoz Ag | Cell culture process |
| US7294484B2 (en) | 2004-08-27 | 2007-11-13 | Wyeth Research Ireland Limited | Production of polypeptides |
| PL1902141T3 (pl) | 2005-06-17 | 2012-12-31 | Baxalta Inc | Kompozycje o działaniu trombolitycznym zawierające ADAMTS13 |
| CA2633306A1 (en) * | 2006-01-04 | 2007-07-12 | Baxter International Inc. | Oligopeptide-free cell culture media |
| AU2008345231B2 (en) * | 2007-12-27 | 2014-09-11 | Takeda Pharmaceutical Company Limited | Cell culture processes |
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2010
- 2010-07-30 US US12/847,921 patent/US8580554B2/en active Active
- 2010-08-02 KR KR1020177017512A patent/KR101885046B1/ko active Active
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- 2010-08-02 PL PL10737916T patent/PL2459697T3/pl unknown
- 2010-08-02 KR KR1020127004996A patent/KR101752789B1/ko active Active
- 2010-08-02 CN CN201080036754.2A patent/CN102549142B/zh active Active
- 2010-08-02 JP JP2012522190A patent/JP5808742B2/ja active Active
- 2010-08-02 ES ES10737916T patent/ES2705206T3/es active Active
- 2010-08-02 DK DK10737916.6T patent/DK2459697T3/en active
- 2010-08-02 CA CA2769354A patent/CA2769354A1/en not_active Abandoned
- 2010-08-02 WO PCT/EP2010/061191 patent/WO2011012725A1/en not_active Ceased
- 2010-08-02 IN IN899DEN2012 patent/IN2012DN00899A/en unknown
- 2010-08-02 EP EP18199855.0A patent/EP3480292A1/en not_active Withdrawn
- 2010-08-02 SI SI201031835T patent/SI2459697T1/sl unknown
- 2010-08-02 PT PT10737916T patent/PT2459697T/pt unknown
- 2010-08-02 NZ NZ597742A patent/NZ597742A/en unknown
- 2010-08-02 HR HRP20182165TT patent/HRP20182165T1/hr unknown
- 2010-08-02 SG SG2012006599A patent/SG178176A1/en unknown
- 2010-08-02 TR TR2019/00213T patent/TR201900213T4/tr unknown
- 2010-08-02 EP EP10737916.6A patent/EP2459697B1/en active Active
- 2010-08-02 EA EA201270213A patent/EA022219B1/ru not_active IP Right Cessation
- 2010-08-02 BR BR112012002137A patent/BR112012002137B8/pt active IP Right Grant
- 2010-08-02 MX MX2012001274A patent/MX2012001274A/es active IP Right Grant
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2012
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2013
- 2013-10-10 US US14/050,710 patent/US9650612B2/en active Active
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2015
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2019
- 2019-01-18 US US16/252,216 patent/US10745672B2/en active Active
Non-Patent Citations (10)
| Title |
|---|
| ANTOINE G. ET AL. "ADAMTS13 gene defects in two brothers with constitutional thrombotic thrombocytopenic purpura and normalization of von Willebrand factor-cleaving protease activity by recombinant human ADAMTS13". BRITISH JOURNAL OF HAEMATOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB LNKD-D0I:10.1046/J.1365-2141.2003.04183.X, vol. 120, no. 5, 1 March 2003 (2003-03-01), pages 821-824, XP002282271, ISSN: 0007-1048, the whole document * |
| BANIK GAUTAM G. ET AL. "Hybridoma growth and antibody production as a function of cell density and specific growth rate in perfusion culture". BIOTECHNOLOGY AND BIOENGINEERING, vol. 48, no. 3, 1995, pages 289-300, XP002599809, ISSN: 0006-3592, the whole document * |
| BATT B.C. ET AL. "INCLINED SEDIMENTATION FOR SELECTIVE RETENTION OF VIABLE HYBRIDOMAS IN A CONTINUOUS SUSPENSION BIOREACTOR". BIOTECHNOLOGY PROGRESS, AMERICAN INSTITUTE OF CHEMICAL ENGINEERS, US LNKD-D0I:10.1021/BP00006A600, vol. 6, 1 January 1990 (1990-01-01), pages 458-464, XP001147277, ISSN: 8756-7938, the whole document * |
| CASTILHO L.R. ET AL. "CELL RETENTION DEVICES FOR SUSPENDED-CELL PERFUSION CULTURES". ADVANCES IN BIOCHEMICAL ENGINEERING, BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 74, 1 January 2002 (2002-01-01), pages 129-169, XP009038515, ISSN: 0724-6145, the whole document * |
| GORENFLO VOLKER M. ET AL. "Optimization of an acoustic cell filter with a novel air-backflush system". BIOTECHNOLOGY PROGRESS, vol. 19, no. 1, January 2003 (2003-01), pages 30-36, XP9138351, ISSN: 8756-7938, the whole document * |
| HILLER G.W. ET AL. "Cell retention-chemostat studies of hybridoma cells: Analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium". BIOTECHNOLOGY AND BIOENGINEERING, vol. 42, no. 2, 1993, pages 185-195, XP002599810, ISSN: 0006-3592, the whole document * |
| PLAIMAUER BARBARA ET AL. "Expression and characterization of recombinant human ADAMTS13". SEMINARS IN HEMATOLOGY, vol. 41, no. 1, January 2004 (2004-01), pages 24-33, XP009138417, ISSN: 0037-1963, the whole document * |
| ROBINSON D.K. EP AL. "Effect of specific growth rates on productivity in continuous open and partial cell retention animal cell bioreactors". JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL LNKD-DOI:10.1016/0168-1656(92)90131-R, vol. 22, no. 1-2, 1 January 1992 (1992-01-01), pages 41-50, XP023944261, ISSN: 0168-1656 [retrieved on 1992-01-01], the whole document * |
| SCHMID G. ET AL. "Continuous hybridoma suspension cultures with and without cell retention: kinetics of growth, metabolism and product formation". JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL LNKD-D0I:10.1016/0168-1656(92)90130-2, vol. 22, no. 1-2, 1 January 1992 (1992-01-01), pages 31-40 XP023944260, ISSN: 0168-1656 [retrieved on 1992-01-01], the whole document * |
| VOISARD D. ET AL. "Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells". BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US LNKD-D0I:10.1002/BIT.10629, vol. 82, no. 7, 30 June 2003 (2003-06-30), pages 751-765, XP002317548, ISSN: 0006-3592, tables * |
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