KR101885046B1 - 연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법 - Google Patents

연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법 Download PDF

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KR101885046B1
KR101885046B1 KR1020177017512A KR20177017512A KR101885046B1 KR 101885046 B1 KR101885046 B1 KR 101885046B1 KR 1020177017512 A KR1020177017512 A KR 1020177017512A KR 20177017512 A KR20177017512 A KR 20177017512A KR 101885046 B1 KR101885046 B1 KR 101885046B1
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cell
cells
culture
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cell culture
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KR20170077286A (ko
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레오폴트 그릴베르거
만프레트 라이터
다니엘 플라이샨데를
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박스알타 인코퍼레이티드
박스앨타 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/006—Cell injection or fusion devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0056—Xeno-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/00051—Methods of production or purification of viral material
    • C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020177017512A 2009-07-31 2010-08-02 연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법 Active KR101885046B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23031309P 2009-07-31 2009-07-31
US61/230,313 2009-07-31
PCT/EP2010/061191 WO2011012725A1 (en) 2009-07-31 2010-08-02 Method of producing a polypeptide or virus of interest in a continuous cell culture

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KR1020127004996A Division KR101752789B1 (ko) 2009-07-31 2010-08-02 연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법

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KR20170077286A KR20170077286A (ko) 2017-07-05
KR101885046B1 true KR101885046B1 (ko) 2018-08-02

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KR1020127004996A Active KR101752789B1 (ko) 2009-07-31 2010-08-02 연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법
KR1020177017512A Active KR101885046B1 (ko) 2009-07-31 2010-08-02 연속 세포 배양에서 목적 폴리펩티드 또는 바이러스를 생산하는 방법

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US (4) US8580554B2 (enExample)
EP (2) EP2459697B1 (enExample)
JP (2) JP5808742B2 (enExample)
KR (2) KR101752789B1 (enExample)
CN (1) CN102549142B (enExample)
AU (1) AU2010277490B2 (enExample)
BR (1) BR112012002137B8 (enExample)
CA (1) CA2769354A1 (enExample)
CO (1) CO6491119A2 (enExample)
DK (1) DK2459697T3 (enExample)
EA (1) EA022219B1 (enExample)
ES (1) ES2705206T3 (enExample)
HR (1) HRP20182165T1 (enExample)
IN (1) IN2012DN00899A (enExample)
MX (2) MX2012001274A (enExample)
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PL (1) PL2459697T3 (enExample)
PT (1) PT2459697T (enExample)
SG (1) SG178176A1 (enExample)
SI (1) SI2459697T1 (enExample)
TR (1) TR201900213T4 (enExample)
WO (1) WO2011012725A1 (enExample)

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EP3327119A1 (en) 2011-11-24 2018-05-30 Genethon Scalable lentiviral vector production system compatible with industrial pharmaceutical applications
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DK2976418T3 (da) 2013-03-19 2021-01-11 Cmc Biologics As Fremgangsmåde til fremstilling af et produkt (f.eks. polypeptid) i en kontinuerlig cellekulturfermenteringsproces
CA2920934C (en) * 2013-08-30 2022-12-06 Glaxosmithkline Biologicals S.A. Large scale production of viruses in cell culture
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
AU2015240353A1 (en) 2014-04-01 2016-11-17 Advantech Bioscience Farmaceutica Ltda. Stable Factor VIII formulations with low sugar-glycine
WO2015149144A2 (en) 2014-04-01 2015-10-08 Advantech Bioscience Farmacêutica Ltda. Stabilization of factor viii without calcium as an excipient
WO2015188224A1 (en) * 2014-06-13 2015-12-17 Csl Limited Improved production of recombinant von willebrand factor in a bioreactor
KR20240173213A (ko) 2016-01-26 2024-12-10 베링거 인겔하임 인터내셔날 게엠베하 연결된 관류 대 연속-유동식 교반-탱크 반응기 세포 배양 시스템
KR102414290B1 (ko) * 2016-04-15 2022-06-29 베링거 인겔하임 인터내셔날 게엠베하 세포 보유 장치 및 방법
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CO6491119A2 (es) 2012-07-31
SG178176A1 (en) 2012-03-29
AU2010277490A1 (en) 2012-02-09
TR201900213T4 (tr) 2019-02-21
US20180051261A1 (en) 2018-02-22
BR112012002137B8 (pt) 2020-05-12
US20140038264A1 (en) 2014-02-06
JP2013500710A (ja) 2013-01-10
EA201270213A1 (ru) 2012-06-29
US8580554B2 (en) 2013-11-12
KR20170077286A (ko) 2017-07-05
US10221400B2 (en) 2019-03-05
PT2459697T (pt) 2019-01-21
US20190153400A1 (en) 2019-05-23
AU2010277490B2 (en) 2014-08-28
JP5808742B2 (ja) 2015-11-10
CN102549142A (zh) 2012-07-04
KR20120038531A (ko) 2012-04-23
WO2011012725A1 (en) 2011-02-03
ES2705206T3 (es) 2019-03-22
HRP20182165T1 (hr) 2019-03-08
JP2015062436A (ja) 2015-04-09
PL2459697T3 (pl) 2019-04-30
KR101752789B1 (ko) 2017-06-30
CA2769354A1 (en) 2011-02-03
EP3480292A1 (en) 2019-05-08
US20110086411A1 (en) 2011-04-14
EA022219B1 (ru) 2015-11-30
CN102549142B (zh) 2014-07-16
WO2011012725A8 (en) 2011-05-05
SI2459697T1 (sl) 2019-03-29
IN2012DN00899A (enExample) 2015-04-03
MX2012001274A (es) 2012-06-01
BR112012002137A2 (pt) 2015-09-15
NZ597742A (en) 2014-02-28
EP2459697A1 (en) 2012-06-06
BR112012002137B1 (pt) 2020-04-14
JP6177260B2 (ja) 2017-08-09
US10745672B2 (en) 2020-08-18
MX353610B (es) 2018-01-18
DK2459697T3 (en) 2019-01-28
EP2459697B1 (en) 2018-10-17
US9650612B2 (en) 2017-05-16

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