CO6491119A2 - Método de producción de un polipéptido o virus de interés en un cultivo celular contínuo - Google Patents
Método de producción de un polipéptido o virus de interés en un cultivo celular contínuoInfo
- Publication number
- CO6491119A2 CO6491119A2 CO12020130A CO12020130A CO6491119A2 CO 6491119 A2 CO6491119 A2 CO 6491119A2 CO 12020130 A CO12020130 A CO 12020130A CO 12020130 A CO12020130 A CO 12020130A CO 6491119 A2 CO6491119 A2 CO 6491119A2
- Authority
- CO
- Colombia
- Prior art keywords
- cell culture
- cell
- culture system
- polypeptide
- interest
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/006—Cell injection or fusion devices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0056—Xeno-free medium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/00051—Methods of production or purification of viral material
- C12N2710/00052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
En la presente descripción se describe un sistema de cultivo celular continuo similar al quimiostato que combina ciertas ventajas de los sistemas abiertos de perfusión y sistemas abiertos de quimiostato para mejorar el cultivo de células de mamíferos, por ejemplo, células genéticamente modificadas, particularmente en medio libre de suero o químicamente definido. El sistema de cultivo continuo descrito en la presente descripción involucra cultivar células de mamíferos en un sistema de cultivo celular continuo, que comprende un dispositivo de retención celular, en donde el sistema de cultivo celular tiene una velocidad de dilución (D) de menos que aproximadamente 2 d-1, y una densidad celular de menos que aproximadamente 2X107 célula/ml. Además, se describe un método para producir un polipéptido y/o virus de interés en un cultivo celular continuo, el método comprende cultivar células de mamíferos que expresan el polipéptido y/o virus de interés en un sistema de cultivo celular continuo, que comprende un dispositivo de retención celular, en donde el sistema de cultivo celular tiene una velocidad de dilución (D) de menos que aproximadamente 2 d-1, y una densidad celular de menos que aproximadamente 2X107 célula/ml; y la recuperación del polipéptido y/o virus de interés a partir del medio del sistema de cultivo celular.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23031309P | 2009-07-31 | 2009-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6491119A2 true CO6491119A2 (es) | 2012-07-31 |
Family
ID=42668436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO12020130A CO6491119A2 (es) | 2009-07-31 | 2012-02-06 | Método de producción de un polipéptido o virus de interés en un cultivo celular contínuo |
Country Status (22)
| Country | Link |
|---|---|
| US (4) | US8580554B2 (es) |
| EP (2) | EP2459697B1 (es) |
| JP (2) | JP5808742B2 (es) |
| KR (2) | KR101885046B1 (es) |
| CN (1) | CN102549142B (es) |
| AU (1) | AU2010277490B2 (es) |
| BR (1) | BR112012002137B8 (es) |
| CA (1) | CA2769354A1 (es) |
| CO (1) | CO6491119A2 (es) |
| DK (1) | DK2459697T3 (es) |
| EA (1) | EA022219B1 (es) |
| ES (1) | ES2705206T3 (es) |
| HR (1) | HRP20182165T1 (es) |
| IN (1) | IN2012DN00899A (es) |
| MX (2) | MX2012001274A (es) |
| NZ (1) | NZ597742A (es) |
| PL (1) | PL2459697T3 (es) |
| PT (1) | PT2459697T (es) |
| SG (1) | SG178176A1 (es) |
| SI (1) | SI2459697T1 (es) |
| TR (1) | TR201900213T4 (es) |
| WO (1) | WO2011012725A1 (es) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL286298B (en) | 2010-07-08 | 2022-08-01 | Baxalta Inc | A method to generate high molecular weight substituted vwf in cell culture |
| HRP20211494T1 (hr) * | 2011-01-17 | 2021-12-24 | F. Hoffmann - La Roche Ag | Uređaj za odvajanje |
| RU2600886C2 (ru) | 2011-05-13 | 2016-10-27 | Октафарма Аг | Способ повышения продуктивности эукариотических клеток в продуцировании рекомбинантного fviii |
| LT2837680T (lt) * | 2011-07-01 | 2020-07-10 | Amgen Inc. | Žinduolių ląstelių kultūra |
| CN203129697U (zh) | 2013-02-05 | 2013-08-14 | 客贝利(厦门)休闲用品有限公司 | 一种帐篷架杆 |
| SI2782997T1 (en) | 2011-11-24 | 2018-04-30 | Genethon | A rolling lentiviral vector manufacturing system compatible with industrial pharmaceutical applications |
| US9574167B2 (en) | 2013-02-15 | 2017-02-21 | Pharyx, Inc. | Methods and apparatus for independent control of product and reactant concentrations |
| SG10201709131UA (en) | 2013-03-08 | 2017-12-28 | Genzyme Corp | Continuous purification of therapeutic proteins |
| US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
| WO2014146933A1 (en) * | 2013-03-19 | 2014-09-25 | Cmc Biologics A/S | A method for producing a product (e.g. polypeptide) in a continuous cell culture fermentation process. |
| JP6851827B2 (ja) * | 2013-08-30 | 2021-03-31 | グラクソスミスクライン バイオロジカルズ ソシエテ アノニム | 細胞培養中でのウイルスの大規模製造 |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| TWI709570B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析法及製法 |
| WO2015149143A2 (en) | 2014-04-01 | 2015-10-08 | Advantech Bioscience Farmacêutica Ltda. | Stable factor viii formulations with low sugar-glycine |
| CN106456720A (zh) | 2014-04-01 | 2017-02-22 | 爱德技术生物科学有限公司 | 在无钙作为赋形剂下因子viii的稳定性 |
| WO2015188224A1 (en) * | 2014-06-13 | 2015-12-17 | Csl Limited | Improved production of recombinant von willebrand factor in a bioreactor |
| CN108884428B (zh) * | 2016-01-26 | 2023-04-11 | 勃林格殷格翰国际公司 | 对连续流动搅拌釜反应器细胞培养系统的连接灌注 |
| SG11201809051YA (en) * | 2016-04-15 | 2018-11-29 | Boehringer Ingelheim Int | Cell retention device and method |
| SI3559203T1 (sl) * | 2016-12-21 | 2021-03-31 | F. Hoffmann-La Roche Ag | Nadzor rasti evkariontskih celic |
| MX2020011614A (es) | 2018-05-01 | 2020-12-09 | Amgen Inc | Anticuerpos con perfiles de glicanos modulados. |
| WO2019239780A1 (ja) * | 2018-06-15 | 2019-12-19 | 富士フイルム株式会社 | 細胞培養方法、細胞培養装置、及び生産物の製造方法 |
| CA3110666A1 (en) | 2018-08-31 | 2020-03-05 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| CN113355297A (zh) * | 2021-06-23 | 2021-09-07 | 吉林冠界生物技术有限公司 | 一种灌流式培养全悬浮mdck细胞生产重组禽流感病毒的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5019512A (en) | 1989-03-17 | 1991-05-28 | Baxter International Inc. | Spin filter for removing substantially cell-free culture medium from suspension cell culture system |
| US5626734A (en) | 1995-08-18 | 1997-05-06 | University Technologies International, Inc. | Filter for perfusion cultures of animal cells and the like |
| WO2002029083A2 (en) | 2000-10-02 | 2002-04-11 | Novo Nordisk A/S | Industrial-scale serum-free production of recombinant proteins in mammalian cells |
| WO2003045995A2 (en) * | 2001-11-28 | 2003-06-05 | Sandoz Gmbh | Cell culture process |
| TWI384069B (zh) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| PL1902141T3 (pl) | 2005-06-17 | 2012-12-31 | Baxalta Inc | Kompozycje o działaniu trombolitycznym zawierające ADAMTS13 |
| KR101423344B1 (ko) * | 2006-01-04 | 2014-07-30 | 백스터 인터내셔널 인코포레이티드 | 올리고펩티드-무함유 세포 배양 배지를 이용하여 단백질을 발현시키는 방법 |
| PT2235197T (pt) * | 2007-12-27 | 2017-10-11 | Baxalta Inc | Processos para cultura de células |
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2010
- 2010-07-30 US US12/847,921 patent/US8580554B2/en active Active
- 2010-08-02 PT PT10737916T patent/PT2459697T/pt unknown
- 2010-08-02 KR KR1020177017512A patent/KR101885046B1/ko active IP Right Grant
- 2010-08-02 SI SI201031835T patent/SI2459697T1/sl unknown
- 2010-08-02 PL PL10737916T patent/PL2459697T3/pl unknown
- 2010-08-02 TR TR2019/00213T patent/TR201900213T4/tr unknown
- 2010-08-02 MX MX2012001274A patent/MX2012001274A/es active IP Right Grant
- 2010-08-02 EP EP10737916.6A patent/EP2459697B1/en active Active
- 2010-08-02 AU AU2010277490A patent/AU2010277490B2/en active Active
- 2010-08-02 JP JP2012522190A patent/JP5808742B2/ja active Active
- 2010-08-02 IN IN899DEN2012 patent/IN2012DN00899A/en unknown
- 2010-08-02 DK DK10737916.6T patent/DK2459697T3/en active
- 2010-08-02 KR KR1020127004996A patent/KR101752789B1/ko active IP Right Grant
- 2010-08-02 WO PCT/EP2010/061191 patent/WO2011012725A1/en active Application Filing
- 2010-08-02 NZ NZ597742A patent/NZ597742A/en unknown
- 2010-08-02 EP EP18199855.0A patent/EP3480292A1/en not_active Withdrawn
- 2010-08-02 BR BR112012002137A patent/BR112012002137B8/pt active IP Right Grant
- 2010-08-02 ES ES10737916T patent/ES2705206T3/es active Active
- 2010-08-02 CA CA2769354A patent/CA2769354A1/en not_active Abandoned
- 2010-08-02 EA EA201270213A patent/EA022219B1/ru not_active IP Right Cessation
- 2010-08-02 CN CN201080036754.2A patent/CN102549142B/zh active Active
- 2010-08-02 SG SG2012006599A patent/SG178176A1/en unknown
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2012
- 2012-01-27 MX MX2014008515A patent/MX353610B/es unknown
- 2012-02-06 CO CO12020130A patent/CO6491119A2/es unknown
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2013
- 2013-10-10 US US14/050,710 patent/US9650612B2/en active Active
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2015
- 2015-01-14 JP JP2015004718A patent/JP6177260B2/ja active Active
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2017
- 2017-04-12 US US15/486,181 patent/US10221400B2/en active Active
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2018
- 2018-12-20 HR HRP20182165TT patent/HRP20182165T1/hr unknown
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2019
- 2019-01-18 US US16/252,216 patent/US10745672B2/en active Active
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