MX2020013633A - Metodos para producir proteinas recombinantes. - Google Patents
Metodos para producir proteinas recombinantes.Info
- Publication number
- MX2020013633A MX2020013633A MX2020013633A MX2020013633A MX2020013633A MX 2020013633 A MX2020013633 A MX 2020013633A MX 2020013633 A MX2020013633 A MX 2020013633A MX 2020013633 A MX2020013633 A MX 2020013633A MX 2020013633 A MX2020013633 A MX 2020013633A
- Authority
- MX
- Mexico
- Prior art keywords
- sup
- cells
- perfusion
- culturing
- interest
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
En ciertas modalidades, esta descripción proporciona métodos novedosos para incrementar la densidad celular viable de un cultivo celular de biorreactor a gran escala N-1, que comprende cultivar una célula hospedera que expresa un polipéptido recombinante de interés en un sistema de cultivo celular no basado en perfusión, y en donde la densidad celular viable se incrementa en al menos 5 × 106 células/mL. En ciertas modalidades, la descripción proporciona métodos novedosos para la producción a gran escala de un polipéptido recombinante de interés, que comprende: (1) cultivar una célula hospedera que expresa un polipéptido recombinante de interés en una etapa N-1 en un sistema de cultivo celular no basado en perfusión, en donde la densidad celular viable se incrementa en al menos 5 × 106 células/mL; y (2) cultivar las células de producción de lote alimentado N en un medio enriquecido con densidad de alta siembra al menos 1.5 × 106 células/mL, en donde las células de producción por lote alimentado N son inoculadas a partir de la etapa N-1 en un sistema de cultivo celular no basado en perfusión.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862693606P | 2018-07-03 | 2018-07-03 | |
| PCT/US2019/040298 WO2020010080A1 (en) | 2018-07-03 | 2019-07-02 | Methods of producing recombinant proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020013633A true MX2020013633A (es) | 2021-03-25 |
Family
ID=67470657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020013633A MX2020013633A (es) | 2018-07-03 | 2019-07-02 | Metodos para producir proteinas recombinantes. |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US20210253996A1 (es) |
| EP (2) | EP3818078B1 (es) |
| JP (2) | JP7419273B2 (es) |
| KR (1) | KR20210027427A (es) |
| CN (1) | CN112839954A (es) |
| AU (1) | AU2019299358A1 (es) |
| BR (1) | BR112020025623A2 (es) |
| CA (1) | CA3104684A1 (es) |
| DK (1) | DK3818078T3 (es) |
| EA (1) | EA202092904A1 (es) |
| FI (1) | FI3818078T3 (es) |
| IL (1) | IL279782A (es) |
| LT (1) | LT3818078T (es) |
| MX (1) | MX2020013633A (es) |
| PL (1) | PL3818078T3 (es) |
| PT (1) | PT3818078T (es) |
| SG (1) | SG11202012582QA (es) |
| WO (1) | WO2020010080A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023242238A1 (en) * | 2022-06-15 | 2023-12-21 | UCB Biopharma SRL | Cell culture processes |
| EP4339274A1 (en) | 2022-09-13 | 2024-03-20 | Sartorius Stedim Biotech GmbH | Method for operating a bioprocess installation for production of a bioproduct |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| US5672347A (en) | 1984-07-05 | 1997-09-30 | Genentech, Inc. | Tumor necrosis factor antagonists and their use |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| DE68919361T2 (de) | 1988-06-21 | 1995-05-24 | Genentech Inc | Therapeutische zusammensetzungen für die behandlung von myocard-infarkten. |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| WO1993004173A1 (en) | 1991-08-14 | 1993-03-04 | Genentech, Inc. | Immunoglobulin variants for specific fc epsilon receptors |
| AU687755B2 (en) | 1992-08-21 | 1998-03-05 | Genentech Inc. | Method for treating an LFA-1-mediated disorder |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| JP3507852B2 (ja) | 1993-12-10 | 2004-03-15 | ジェネンテク，インコーポレイテッド | アレルギー診断法および抗アレルギー性治療剤のスクリーニング法 |
| WO1995019181A1 (en) | 1994-01-18 | 1995-07-20 | Genentech, Inc. | A METHOD OF TREATMENT OF PARASITIC INFECTION USING IgE ANTAGONISTS |
| CA2181787A1 (en) | 1994-03-03 | 1995-09-08 | Claire M. Doerschuk | Anti-il-8 monoclonal antibodies for treatment of inflammatory disorders |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| WO1996040210A1 (en) | 1995-06-07 | 1996-12-19 | Imclone Systems Incorporated | Antibody and antibody fragments for inhibiting the growth of tumors |
| ES2183131T3 (es) | 1996-01-23 | 2003-03-16 | Genentech Inc | Anticuerpos anti-cd18 utilizados contra el ictus cerebral. |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| PT941344E (pt) | 1996-11-27 | 2004-10-29 | Genentech Inc | Anticorpos anti-cd11a humanizados |
| ES2236634T3 (es) | 1997-04-07 | 2005-07-16 | Genentech, Inc. | Anticuerpos anti-vegf. |
| ES2293682T5 (es) | 1997-05-15 | 2011-11-17 | Genentech, Inc. | Anticuerpo anti-apo2. |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| EP1226177B1 (en) | 1999-10-29 | 2008-07-09 | Genentech, Inc. | Anti-prostate stem cell antigen (psca) antibody compositions and methods of use |
| TWI374935B (en) * | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
| NZ575328A (en) * | 2006-09-13 | 2012-06-29 | Abbott Lab | Cell culture improvements |
| PL2673296T3 (pl) * | 2011-02-07 | 2019-03-29 | Cerenis Therapeutics Holding Sa | Kompleksy lipoproteinowe i ich wytwarzanie oraz ich zastosowania |
| WO2015198451A1 (ja) | 2014-06-26 | 2015-12-30 | 楽天株式会社 | 情報処理装置、情報処理方法及び情報処理プログラム |
-
2019
- 2019-07-02 EP EP19745845.8A patent/EP3818078B1/en active Active
- 2019-07-02 SG SG11202012582QA patent/SG11202012582QA/en unknown
- 2019-07-02 CN CN201980044908.3A patent/CN112839954A/zh active Pending
- 2019-07-02 EP EP24153543.4A patent/EP4368699A2/en active Pending
- 2019-07-02 US US17/252,163 patent/US20210253996A1/en active Pending
- 2019-07-02 FI FIEP19745845.8T patent/FI3818078T3/fi active
- 2019-07-02 BR BR112020025623-4A patent/BR112020025623A2/pt unknown
- 2019-07-02 PT PT197458458T patent/PT3818078T/pt unknown
- 2019-07-02 PL PL19745845.8T patent/PL3818078T3/pl unknown
- 2019-07-02 AU AU2019299358A patent/AU2019299358A1/en active Pending
- 2019-07-02 KR KR1020217003056A patent/KR20210027427A/ko unknown
- 2019-07-02 WO PCT/US2019/040298 patent/WO2020010080A1/en unknown
- 2019-07-02 EA EA202092904A patent/EA202092904A1/ru unknown
- 2019-07-02 MX MX2020013633A patent/MX2020013633A/es unknown
- 2019-07-02 JP JP2020573229A patent/JP7419273B2/ja active Active
- 2019-07-02 LT LTEPPCT/US2019/040298T patent/LT3818078T/lt unknown
- 2019-07-02 CA CA3104684A patent/CA3104684A1/en active Pending
- 2019-07-02 DK DK19745845.8T patent/DK3818078T3/da active
-
2020
- 2020-12-25 IL IL279782A patent/IL279782A/en unknown
-
2024
- 2024-01-10 JP JP2024001657A patent/JP2024050590A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| PL3818078T3 (pl) | 2024-04-29 |
| EP3818078B1 (en) | 2024-02-28 |
| BR112020025623A2 (pt) | 2021-03-23 |
| EA202092904A1 (ru) | 2021-04-22 |
| JP7419273B2 (ja) | 2024-01-22 |
| DK3818078T3 (da) | 2024-05-21 |
| EP3818078A1 (en) | 2021-05-12 |
| SG11202012582QA (en) | 2021-01-28 |
| PT3818078T (pt) | 2024-04-18 |
| FI3818078T3 (fi) | 2024-05-14 |
| CN112839954A (zh) | 2021-05-25 |
| WO2020010080A1 (en) | 2020-01-09 |
| JP2024050590A (ja) | 2024-04-10 |
| IL279782A (en) | 2021-03-01 |
| AU2019299358A1 (en) | 2021-02-18 |
| LT3818078T (lt) | 2024-04-25 |
| US20210253996A1 (en) | 2021-08-19 |
| CA3104684A1 (en) | 2020-01-09 |
| EP4368699A2 (en) | 2024-05-15 |
| JP2021529527A (ja) | 2021-11-04 |
| KR20210027427A (ko) | 2021-03-10 |
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