JP5782200B2 - ウシ及びブタ精原幹細胞の培養のためのフィーダーフリー法 - Google Patents

ウシ及びブタ精原幹細胞の培養のためのフィーダーフリー法 Download PDF

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JP5782200B2
JP5782200B2 JP2014557708A JP2014557708A JP5782200B2 JP 5782200 B2 JP5782200 B2 JP 5782200B2 JP 2014557708 A JP2014557708 A JP 2014557708A JP 2014557708 A JP2014557708 A JP 2014557708A JP 5782200 B2 JP5782200 B2 JP 5782200B2
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マイケル オートレイ,ジョン
マイケル オートレイ,ジョン
ジョーン オートレイ,メリッサ
ジョーン オートレイ,メリッサ
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ワシントン ステイト ユニバーシティ
ワシントン ステイト ユニバーシティ
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JP2014557708A 2012-02-14 2013-02-11 ウシ及びブタ精原幹細胞の培養のためのフィーダーフリー法 Expired - Fee Related JP5782200B2 (ja)

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US201261598437P 2012-02-14 2012-02-14
US61/598,437 2012-02-14
PCT/US2013/025537 WO2013122864A1 (en) 2012-02-14 2013-02-11 Feeder-free method for culture of bovine and porcine spermatogonial stem cells

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JP2015508653A JP2015508653A (ja) 2015-03-23
JP5782200B2 true JP5782200B2 (ja) 2015-09-24

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MX357280B (es) 2012-02-14 2018-07-03 Univ Washington State Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino.
US9670458B2 (en) * 2013-07-13 2017-06-06 Aggenetics, Inc. Methods for spermatogonial stem cell (SSC) transfer
KR101777160B1 (ko) * 2014-03-31 2017-09-11 강원대학교산학협력단 포유동물 유래 정원줄기세포의 분리방법
KR101618237B1 (ko) * 2014-07-09 2016-05-09 건국대학교 산학협력단 개의 정원줄기세포를 체외배양하는 방법
CN104830756A (zh) * 2015-05-07 2015-08-12 湖州师范学院 翘嘴红鲌精原干细胞分离培养方法
CN105316282B (zh) * 2015-12-09 2018-10-30 中国水产科学研究院长江水产研究所 一种达氏鲟精原细胞培养液及应用
US20170258495A1 (en) 2016-02-29 2017-09-14 The Curators Of The University Of Missouri Medium supplement to increase the efficiency of oocyte maturation and embryo culture in vitro
WO2018160920A1 (en) * 2017-03-02 2018-09-07 Orig3N, Inc. Systems and methods of assisted reproduction and prevention of genetic defects in offspring using induced pluripotent stem cells
CN109055306A (zh) * 2018-06-25 2018-12-21 佛山科学技术学院 一种无饲养层无血清培养小鼠精原干细胞的体系及方法
CN109182269B (zh) * 2018-07-26 2022-07-05 佛山科学技术学院 一种使精原干细胞高效向神经细胞分化的培养体系和方法
CN109402045A (zh) * 2018-10-15 2019-03-01 卢克焕 一种水牛精原干细胞样细胞的体外培养及传代方法
CN109385395A (zh) * 2018-10-15 2019-02-26 卢克焕 一种用于体外培养的水牛精原干细胞样细胞的纯化方法
US20210386792A1 (en) * 2018-12-19 2021-12-16 Dong Ha Bhang A method for increasing population of spermatogonial stem cells
US20230287340A1 (en) * 2020-07-27 2023-09-14 Washington State University Methods for spermatogonial culture
CN113957039A (zh) * 2021-09-06 2022-01-21 西南民族大学 一种冷冻保存的牛生精细胞体外培养方法
WO2024177532A1 (ru) * 2023-02-20 2024-08-29 Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" Тест-система для оценки секреторной активности модельных клеток
WO2024218715A1 (en) 2023-04-19 2024-10-24 Btl Healthcare Technologies A.S. Culture media based on protein hydrolysate and a process for preparing thereof
CN116904396A (zh) * 2023-08-31 2023-10-20 中国农业科学院兰州畜牧与兽药研究所 牦牛睾丸间质细胞的分离纯化方法
US12668774B2 (en) 2023-10-12 2026-06-30 Btl Healthcare Technologies A.S. Product, system and method of cell cultivation

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5858354A (en) 1991-12-06 1999-01-12 The Trustees Of The University Of Pennsylvania Repopulation of testicular Seminiferous tubules with foreign cells, corresponding resultant germ cells, and corresponding resultant animals and progeny
PT1572984E (pt) * 2002-12-16 2016-06-03 Technion Res & Dev Foundation Sistema de cultura para células estaminais embrionárias humanas sem células alimentadoras e sem xeno
EP1616944A4 (en) * 2003-04-15 2007-10-10 Univ Kyoto PROCESS FOR THE GROWING OF SPERMASTAMM CELLS IN VITRO, SPERMASTAMM CELLS TREATED WITH THIS METHOD AND MEDIATIVE ADDITIVE KIT TO BE USED IN VITRO SPERMASTAMM CELLS
AU2004318461B9 (en) * 2004-03-30 2009-02-26 Kyoto University Process for producing multipotential stem cell originating in testoid cell
WO2005105984A2 (en) * 2004-04-12 2005-11-10 The Trustees Of The University Of Pennsylvania Culture conditions and growth factors affecting fate determination, self-renewal and expansion of mouse spermatogonial stem cells
JP2006115767A (ja) * 2004-10-21 2006-05-11 Kyoto Univ フィーダー細胞の不在下で精原幹細胞を増殖させる方法
WO2006070370A2 (en) * 2004-12-29 2006-07-06 Hadasit Medical Research Services & Development Limited Stem cells culture systems
CA2694956C (en) * 2007-07-31 2017-12-19 Lifescan, Inc. Pluripotent stem cell differentiation by using human feeder cells
KR20100093513A (ko) * 2007-08-07 2010-08-25 프라임젠 바이오테크 엘엘씨 생식계 줄기 세포의 분리, 특성 규명 및 증식
CN102131919B (zh) * 2008-03-31 2017-05-03 东方酵母工业株式会社 一种增殖多能性干细胞的方法
US7939322B2 (en) * 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
KR20120120157A (ko) * 2009-11-05 2012-11-01 조니 융-치옹 초우 생식줄기세포 보관 시스템
CN101818127B (zh) * 2010-03-31 2011-12-28 安徽农业大学 一种小鼠精原干细胞分离培养方法
CN102031242A (zh) * 2010-11-19 2011-04-27 西北农林科技大学 用于提高动物精原干细胞增殖率的培养液体系和使用方法
CN102344909B (zh) * 2011-10-20 2013-09-11 上海交通大学医学院附属仁济医院 一种分离人精原干细胞的方法
MX357280B (es) * 2012-02-14 2018-07-03 Univ Washington State Método sin alimentadoras para cultivo de células madre espermatogonias de bovino y porcino.
AU2012100720A4 (en) * 2012-05-22 2012-06-14 Kaul, Gautam Dr Immunomagnetic microbeads for enrichment of CD9+ spermatogonial stem cells

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