JP5777169B2 - 新規な蛍光色素およびその使用 - Google Patents

新規な蛍光色素およびその使用 Download PDF

Info

Publication number
JP5777169B2
JP5777169B2 JP2012520764A JP2012520764A JP5777169B2 JP 5777169 B2 JP5777169 B2 JP 5777169B2 JP 2012520764 A JP2012520764 A JP 2012520764A JP 2012520764 A JP2012520764 A JP 2012520764A JP 5777169 B2 JP5777169 B2 JP 5777169B2
Authority
JP
Japan
Prior art keywords
group
atom
dye
carbon
bond
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2012520764A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012533654A (ja
Inventor
ハシェム アカバン−タフティ，
ハシェム アカバン−タフティ，
シルバ， レヌーカ デ
シルバ， レヌーカ デ
グオピン ウォン，
グオピン ウォン，
ロバート エー． アイクホルト，
ロバート エー． アイクホルト，
ラビンダー ケイ． グプタ，
ラビンダー ケイ． グプタ，
ラクシュミ エス． カーヌマール，
ラクシュミ エス． カーヌマール，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of JP2012533654A publication Critical patent/JP2012533654A/ja
Application granted granted Critical
Publication of JP5777169B2 publication Critical patent/JP5777169B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/32—One oxygen, sulfur or nitrogen atom
    • C07D239/42—One nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B7/00—Indigoid dyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Thiazole And Isothizaole Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
JP2012520764A 2009-07-16 2010-07-15 新規な蛍光色素およびその使用 Active JP5777169B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22598509P 2009-07-16 2009-07-16
US61/225,985 2009-07-16
PCT/US2010/042063 WO2011008912A1 (en) 2009-07-16 2010-07-15 Novel fluorescent dyes and uses thereof

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015107038A Division JP2015165031A (ja) 2009-07-16 2015-05-27 新規な蛍光色素およびその使用

Publications (2)

Publication Number Publication Date
JP2012533654A JP2012533654A (ja) 2012-12-27
JP5777169B2 true JP5777169B2 (ja) 2015-09-09

Family

ID=43218117

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2012520764A Active JP5777169B2 (ja) 2009-07-16 2010-07-15 新規な蛍光色素およびその使用
JP2015107038A Withdrawn JP2015165031A (ja) 2009-07-16 2015-05-27 新規な蛍光色素およびその使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2015107038A Withdrawn JP2015165031A (ja) 2009-07-16 2015-05-27 新規な蛍光色素およびその使用

Country Status (12)

Country Link
US (2) US8367842B2 (enExample)
EP (1) EP2454329B1 (enExample)
JP (2) JP5777169B2 (enExample)
KR (1) KR101809758B1 (enExample)
CN (1) CN102498177B (enExample)
AU (1) AU2010273431B2 (enExample)
BR (1) BR112012001043A2 (enExample)
CA (1) CA2768352C (enExample)
IN (1) IN2012DN00454A (enExample)
NZ (1) NZ597512A (enExample)
SG (1) SG177676A1 (enExample)
WO (1) WO2011008912A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5905402B2 (ja) * 2010-03-11 2016-04-20 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ シアノベンゾチアゾール化合物を使用する生物発光アッセイ
CN102937576B (zh) * 2012-06-26 2015-10-21 广东德美精细化工股份有限公司 一种织物除油剂的防沾性能的评价方法
EP2719703A1 (en) * 2012-10-12 2014-04-16 Ecole Polytechnique Fédérale de Lausanne (EPFL) Precursor molecule for the synthesis of D-luciferin
CN102942537B (zh) * 2012-11-09 2014-07-02 浙江大学 一种用作pH荧光探针的苯并噻唑-苯胺类化合物及其制备方法
US9817929B1 (en) 2014-06-04 2017-11-14 Calypto Design Systems, Inc. Formal verification using microtransactions
EP4641204A2 (en) 2016-04-06 2025-10-29 Sony Group Corporation Ultra bright dimeric or polymeric dyes with spacing linker groups
US10400264B2 (en) 2016-12-01 2019-09-03 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
JP7563668B2 (ja) * 2018-06-05 2024-10-08 プロメガ コーポレイション 熱安定性ルシフェリンと共に生物発光アッセイに使用するためのルシフェラーゼ酵素
JP7580689B2 (ja) 2018-06-27 2024-11-12 ソニーグループ株式会社 デオキシリボースを含むリンカー基を有するポリマー色素
CN112399996A (zh) * 2018-07-13 2021-02-23 索尼公司 具有包含有机磷酸酯单元的主链的聚合的染料
CN110724114B (zh) * 2018-07-16 2022-10-21 北京工商大学 一种检测亚硫酸氢根及硫化氢的荧光探针
CN109633151B (zh) * 2018-12-26 2022-03-11 西北农林科技大学 一种肠炎沙门氏菌检测方法、试纸条及应用
CN110361378B (zh) * 2019-07-25 2021-04-02 浙江大学 基于荧光素乙酯的orac抗氧化活性评价方法及荧光指示剂
WO2021061057A2 (en) * 2019-09-26 2021-04-01 Vidyasirimedhi Institute Of Science And Technology Luciferin derivatives and a method for synthesis thereof
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
EP4310074A4 (en) 2021-03-19 2025-03-26 Kanto Denka Kogyo Co., Ltd. D-luciferin, d-luciferin derivative, precursor of these compounds and method for producing these compounds
CN116046735B (zh) * 2022-12-26 2025-08-22 南京大学 一种利用染料组成的传感器阵列检测全氟化合物的方法
CN119462681B (zh) * 2024-10-16 2025-09-30 聊城柏莱斯生物科技有限公司 一种荧光定量pcr参比染料及其制备方法与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3537877A1 (de) * 1985-10-24 1987-04-30 Geiger Reinhard Luciferin-derivate und immunoassays unter einsatz derartiger luciferin-derivate
DE68928412T2 (de) * 1988-07-08 1998-05-20 Jbl Scient Inc Herstellung und verwendung von fluoreszierenden benzothiazolderivaten
US6140051A (en) * 1997-07-21 2000-10-31 Promega Biosciences, Inc. Fluorescent dibenzazole derivatives and methods related thereto
US7413536B1 (en) * 1999-09-14 2008-08-19 Xenoport, Inc. Substrates and screening methods for transport proteins
JP4354280B2 (ja) * 2002-03-01 2009-10-28 イミューノメディクス、インコーポレイテッド Ｒｓ７抗体
US7541440B2 (en) * 2002-09-30 2009-06-02 Immunomedics, Inc. Chimeric, human and humanized anti-granulocyte antibodies and methods of use
CN100572555C (zh) * 2002-10-23 2009-12-23 犹他大学研究基金会 使用饱和染料的扩增子解链分析
DE10302787A1 (de) * 2003-01-24 2004-08-12 Schering Ag Hydrophile, Thiol-reaktive Cyaninfarbstoffe und deren Konjugate mit Biomolekülen für die Fluoreszenzdiagnostik
JP2006219381A (ja) * 2005-02-08 2006-08-24 Univ Of Electro-Communications 複素環化合物及び発光甲虫ルシフェラーゼ発光系用発光基質
EP3312292B1 (en) * 2005-05-31 2019-09-25 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US20100190180A1 (en) * 2005-07-06 2010-07-29 Heidi Kay Materials and Methods for Screening, Diagnosis and Prognosis of Conditions Associated With Stat Protein Expression
KR100704007B1 (ko) * 2006-01-17 2007-04-04 한국과학기술연구원 화학발광 및 형광을 이용한 신속진단키트 및 이의 사용방법
JP5194258B2 (ja) * 2006-03-27 2013-05-08 国立大学法人電気通信大学 複素環化合物及び発光方法
CN101784897A (zh) * 2007-05-23 2010-07-21 应用生物系统有限公司 通过从活化的化学发光的基质将能量转移至能量受体染料来检测生物分子的试剂、试剂盒和方法
CN101270105B (zh) * 2008-04-08 2011-09-14 杨得锁 用于酶活性分析和酶抑制剂筛选的香豆素类化合物及合成方法
US8551721B2 (en) * 2008-09-09 2013-10-08 Promega Corporation Bioluminescent detection of cyanohydroxy benzothiazole compounds

Also Published As

Publication number Publication date
US8772501B2 (en) 2014-07-08
HK1168122A1 (en) 2012-12-21
BR112012001043A2 (pt) 2016-03-15
AU2010273431A2 (en) 2012-03-01
US20110014599A1 (en) 2011-01-20
US8367842B2 (en) 2013-02-05
AU2010273431B2 (en) 2016-08-25
EP2454329B1 (en) 2018-04-18
EP2454329A1 (en) 2012-05-23
KR101809758B1 (ko) 2017-12-15
CN102498177A (zh) 2012-06-13
WO2011008912A1 (en) 2011-01-20
CN102498177B (zh) 2014-12-24
JP2012533654A (ja) 2012-12-27
SG177676A1 (en) 2012-02-28
KR20120037486A (ko) 2012-04-19
NZ597512A (en) 2014-03-28
CA2768352C (en) 2016-10-11
IN2012DN00454A (enExample) 2015-05-15
JP2015165031A (ja) 2015-09-17
CA2768352A1 (en) 2011-01-20
US20130131324A1 (en) 2013-05-23
AU2010273431A1 (en) 2012-02-02

Similar Documents

Publication Publication Date Title
JP5777169B2 (ja) 新規な蛍光色素およびその使用
US6133445A (en) Rigidized trimethine cyanine dyes
US6686145B1 (en) Rigidized trimethine cyanine dyes
JP4790598B2 (ja) メソ置換シアニン色素標識化試薬
JP2009510198A (ja) 紫色レーザー励起性色素及びその使用方法
US6750346B2 (en) Stable near-infrared (NIR) marker dyes based on benzopyrylium-polymethines
JP2003532745A (ja) エネルギー伝達アッセイ法および試薬
US5981747A (en) Monomethine cyanines rigidized by a two-carbon chain
JP4659213B2 (ja) エネルギー転移色素
US8604204B2 (en) Fluorescent dyes
JPH09100416A (ja) 固定化モノメチンシアニン染料
EP1163372A1 (en) Chemically reactive plane-rigidized cyanine dyes and their derivatives
HK1168122B (en) Novel fluorescent dyes and uses thereof
US20040143122A1 (en) Synthetic molecules for labeling histidine-rich proteins
US6967250B1 (en) Energy transfer dyes
US6979575B1 (en) Fluorochromes for labelling biomolecules and polymer particles and bioanalytical and screening methods based thereon

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130701

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20140527

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140529

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140829

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150130

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150527

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150604

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150701

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150702

R150 Certificate of patent or registration of utility model

Ref document number: 5777169

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250