AU2010273431B2 - Novel fluorescent dyes and uses thereof - Google Patents

Novel fluorescent dyes and uses thereof Download PDF

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Publication number
AU2010273431B2
AU2010273431B2 AU2010273431A AU2010273431A AU2010273431B2 AU 2010273431 B2 AU2010273431 B2 AU 2010273431B2 AU 2010273431 A AU2010273431 A AU 2010273431A AU 2010273431 A AU2010273431 A AU 2010273431A AU 2010273431 B2 AU2010273431 B2 AU 2010273431B2
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AU
Australia
Prior art keywords
dye
carbon
bonds
group
formula
Prior art date
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Active
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AU2010273431A
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English (en)
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AU2010273431A1 (en
AU2010273431A2 (en
Inventor
Hashem Akhavan-Tafti
Renuka De Silva
Robert A. Eickholt
Ravinder K. Gupta
Lakshmi S. Kaanumalle
Guoping Wang
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Beckman Coulter Inc
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Beckman Coulter Inc
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Publication date
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Publication of AU2010273431A1 publication Critical patent/AU2010273431A1/en
Publication of AU2010273431A2 publication Critical patent/AU2010273431A2/en
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Publication of AU2010273431B2 publication Critical patent/AU2010273431B2/en
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Classifications

    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00Other synthetic dyes of known constitution
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D239/00Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/32One oxygen, sulfur or nitrogen atom
    • C07D239/42One nitrogen atom
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D417/00Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09BORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B7/00Indigoid dyes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/535Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Thiazole And Isothizaole Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
AU2010273431A 2009-07-16 2010-07-15 Novel fluorescent dyes and uses thereof Active AU2010273431B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US22598509P 2009-07-16 2009-07-16
US61/225,985 2009-07-16
PCT/US2010/042063 WO2011008912A1 (en) 2009-07-16 2010-07-15 Novel fluorescent dyes and uses thereof

Publications (3)

Publication Number Publication Date
AU2010273431A1 AU2010273431A1 (en) 2012-02-02
AU2010273431A2 AU2010273431A2 (en) 2012-03-01
AU2010273431B2 true AU2010273431B2 (en) 2016-08-25

Family

ID=43218117

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010273431A Active AU2010273431B2 (en) 2009-07-16 2010-07-15 Novel fluorescent dyes and uses thereof

Country Status (12)

Country Link
US (2) US8367842B2 (enExample)
EP (1) EP2454329B1 (enExample)
JP (2) JP5777169B2 (enExample)
KR (1) KR101809758B1 (enExample)
CN (1) CN102498177B (enExample)
AU (1) AU2010273431B2 (enExample)
BR (1) BR112012001043A2 (enExample)
CA (1) CA2768352C (enExample)
IN (1) IN2012DN00454A (enExample)
NZ (1) NZ597512A (enExample)
SG (1) SG177676A1 (enExample)
WO (1) WO2011008912A1 (enExample)

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JP5905402B2 (ja) * 2010-03-11 2016-04-20 プロメガ コーポレイションPromega Corporation シアノベンゾチアゾール化合物を使用する生物発光アッセイ
CN102937576B (zh) * 2012-06-26 2015-10-21 广东德美精细化工股份有限公司 一种织物除油剂的防沾性能的评价方法
EP2719703A1 (en) * 2012-10-12 2014-04-16 Ecole Polytechnique Fédérale de Lausanne (EPFL) Precursor molecule for the synthesis of D-luciferin
CN102942537B (zh) * 2012-11-09 2014-07-02 浙江大学 一种用作pH荧光探针的苯并噻唑-苯胺类化合物及其制备方法
US9817929B1 (en) 2014-06-04 2017-11-14 Calypto Design Systems, Inc. Formal verification using microtransactions
KR20180133890A (ko) 2016-04-06 2018-12-17 소니 주식회사 스페이싱 링커 그룹을 포함하는 초고명도 이량체성 또는 중합체성 염료
EP3548470B1 (en) 2016-12-01 2023-11-01 Promega Corporation 5,5-disubstituted luciferins and their use in luciferase-based assays
JP7563668B2 (ja) * 2018-06-05 2024-10-08 プロメガ コーポレイション 熱安定性ルシフェリンと共に生物発光アッセイに使用するためのルシフェラーゼ酵素
KR102854332B1 (ko) 2018-06-27 2025-09-03 소니그룹주식회사 디옥시리보오스를 포함하는 링커 군을 갖는 중합체성 염료
WO2020014634A1 (en) * 2018-07-13 2020-01-16 Sony Corporation Polymeric dyes having a backbone comprising organophosphate units
CN110724114B (zh) * 2018-07-16 2022-10-21 北京工商大学 一种检测亚硫酸氢根及硫化氢的荧光探针
CN109633151B (zh) * 2018-12-26 2022-03-11 西北农林科技大学 一种肠炎沙门氏菌检测方法、试纸条及应用
CN110361378B (zh) * 2019-07-25 2021-04-02 浙江大学 基于荧光素乙酯的orac抗氧化活性评价方法及荧光指示剂
JP7448993B2 (ja) * 2019-09-26 2024-03-13 ウィタヤシリメティ インスティチュート オブ サイエンス アンド テクノロジー ルシフェリン誘導体及びその合成方法
EP4256078A1 (en) 2020-12-07 2023-10-11 Sony Group Corporation Spacing linker group design for brightness enhancement in dimeric or polymeric dyes
WO2022196819A1 (ja) * 2021-03-19 2022-09-22 関東電化工業株式会社 D-ルシフェリン及びd-ルシフェリン誘導体、並びにこれら化合物の前駆体、並びにこれらの製造方法
CN116046735B (zh) * 2022-12-26 2025-08-22 南京大学 一种利用染料组成的传感器阵列检测全氟化合物的方法
CN119462681B (zh) * 2024-10-16 2025-09-30 聊城柏莱斯生物科技有限公司 一种荧光定量pcr参比染料及其制备方法与应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5098828A (en) * 1985-10-24 1992-03-24 Reinhard Geiger D-luciferin derivatives, their application and processes for the detection of ligands marked with an enzyme in the determination of biochemical substances
US5424440A (en) * 1988-07-08 1995-06-13 Jbl Scientific, Inc. Fluorescent benzothiazole derivatives
WO1999003849A1 (en) * 1997-07-21 1999-01-28 Promega Biosciences, Inc. Fluorescent dibenzazole derivatives and methods related thereto

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AU7703400A (en) * 1999-09-14 2001-04-17 Xenoport, Inc. Substrates and screening methods for transport proteins
ES2871905T3 (es) * 2002-03-01 2021-11-02 Immunomedics Inc Inmunoconjugado que comprende anticuerpos de RS7 humanizados
US7541440B2 (en) * 2002-09-30 2009-06-02 Immunomedics, Inc. Chimeric, human and humanized anti-granulocyte antibodies and methods of use
CN100572555C (zh) * 2002-10-23 2009-12-23 犹他大学研究基金会 使用饱和染料的扩增子解链分析
DE10302787A1 (de) * 2003-01-24 2004-08-12 Schering Ag Hydrophile, Thiol-reaktive Cyaninfarbstoffe und deren Konjugate mit Biomolekülen für die Fluoreszenzdiagnostik
JP2006219381A (ja) * 2005-02-08 2006-08-24 Univ Of Electro-Communications 複素環化合物及び発光甲虫ルシフェラーゼ発光系用発光基質
WO2006130551A2 (en) 2005-05-31 2006-12-07 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US20100190180A1 (en) * 2005-07-06 2010-07-29 Heidi Kay Materials and Methods for Screening, Diagnosis and Prognosis of Conditions Associated With Stat Protein Expression
KR100704007B1 (ko) * 2006-01-17 2007-04-04 한국과학기술연구원 화학발광 및 형광을 이용한 신속진단키트 및 이의 사용방법
WO2007116687A1 (ja) * 2006-03-27 2007-10-18 The University Of Electro-Communications 複素環化合物及び発光方法
ATE522811T1 (de) * 2007-05-23 2011-09-15 Life Technologies Corp Reagentien, kits und verfahren zum nachweis biologischer moleküle mittels energieübertragung von einem aktivierten chemilumineszenzsubstrat auf einen energieakzeptor-farbstoff
CN101270105B (zh) * 2008-04-08 2011-09-14 杨得锁 用于酶活性分析和酶抑制剂筛选的香豆素类化合物及合成方法
US8551721B2 (en) 2008-09-09 2013-10-08 Promega Corporation Bioluminescent detection of cyanohydroxy benzothiazole compounds

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US5098828A (en) * 1985-10-24 1992-03-24 Reinhard Geiger D-luciferin derivatives, their application and processes for the detection of ligands marked with an enzyme in the determination of biochemical substances
US5424440A (en) * 1988-07-08 1995-06-13 Jbl Scientific, Inc. Fluorescent benzothiazole derivatives
WO1999003849A1 (en) * 1997-07-21 1999-01-28 Promega Biosciences, Inc. Fluorescent dibenzazole derivatives and methods related thereto

Also Published As

Publication number Publication date
CA2768352A1 (en) 2011-01-20
WO2011008912A1 (en) 2011-01-20
CN102498177A (zh) 2012-06-13
US8772501B2 (en) 2014-07-08
CN102498177B (zh) 2014-12-24
JP2012533654A (ja) 2012-12-27
AU2010273431A1 (en) 2012-02-02
HK1168122A1 (en) 2012-12-21
KR20120037486A (ko) 2012-04-19
IN2012DN00454A (enExample) 2015-05-15
EP2454329B1 (en) 2018-04-18
NZ597512A (en) 2014-03-28
US8367842B2 (en) 2013-02-05
KR101809758B1 (ko) 2017-12-15
AU2010273431A2 (en) 2012-03-01
JP2015165031A (ja) 2015-09-17
CA2768352C (en) 2016-10-11
BR112012001043A2 (pt) 2016-03-15
US20130131324A1 (en) 2013-05-23
US20110014599A1 (en) 2011-01-20
JP5777169B2 (ja) 2015-09-09
SG177676A1 (en) 2012-02-28
EP2454329A1 (en) 2012-05-23

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