JP7448993B2 - ルシフェリン誘導体及びその合成方法 - Google Patents
ルシフェリン誘導体及びその合成方法 Download PDFInfo
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- JP7448993B2 JP7448993B2 JP2022519495A JP2022519495A JP7448993B2 JP 7448993 B2 JP7448993 B2 JP 7448993B2 JP 2022519495 A JP2022519495 A JP 2022519495A JP 2022519495 A JP2022519495 A JP 2022519495A JP 7448993 B2 JP7448993 B2 JP 7448993B2
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- luciferin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
- C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
- C07D277/62—Benzothiazoles
- C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
ルシフェリン誘導体は、以下の構造からなり、
ルシフェリン誘導体の合成方法は、
ベンゾキノンを得るため、熱安定性デハロゲナーゼ、ラジカル捕捉酵素群、ポリフェノールオキシダーゼ群、及びFADH2生成システムを使用することによってバッファー溶液中においてフェノール誘導体基質との間の第1の反応を行い、
該FADH2生成システムは、熱安定性デハロゲナーゼのFADH2基質を提供するステップ、並びにルシフェリン誘導体を得るためベンゾキノンとD-システインとの間の第2の反応を行うステップを含む。
直接の反応のためFADH2を含む、第1のFADH2生成システム、
NADH、FAD、及びフラビンレダクターゼ群を含み、NADHは、FADからFADH2を産生するための還元剤、かつフラビンレダクターゼ群の基質である、第2のFADH2生成システム、
G-6-PD、グルコース-6-ホスフェート、NAD+、フラビンレダクターゼ群、及びFADを含み、グルコース-6-ホスフェート及びNAD+はNADHを産生するためのG-6-PDの基質であり、NADHは後にFADH2を産生するための還元剤、かつフラビンレダクターゼ群の基質である、第3のFADH2生成システム、
GDH、グルコース、NAD+、フラビンレダクターゼ群、及びFADを含み、グルコース及びNAD+はNADHを産生するためのGDHの基質であり、さらに、還元剤、かつフラビンレダクターゼ群の基質であるNADHは、FADをFADH2に変換する、第4のFADH2生成システム、
FDH、ギ酸又はホルマート、NAD+、フラビンレダクターゼ群、及びFADを含み、ギ酸及びNAD+はNADHを産生するためのFDHの基質であり、そして、還元剤、かつフラビンレダクターゼ群の基質であるNADHは、フラビンレダクターゼの反応によってFADをFADH2に変換する、第5のFADH2生成システム、のシステムのいずれか1つから選択される。
1.ホタルルシフェラーゼと反応させたときのルシフェリン誘導体の生物発光/発光
本開示のルシフェリン誘導体とホタルルシフェラーゼとの反応によって光を放射することができる。発光波長は、分光蛍光光度計を生物発光モードで使用して、ルシフェリン誘導体がホタルルシフェラーゼの基質となることができるか否かについて生物発光特性を証明するため測定することができる。
QTOF質量分析計を使用することから、合成したルシフェリン誘導体である7’-ヨードルシフェリン(C11H7IN2O3S2)、4,7’-ジフルオロルシフェリン(C11H6F2N2O3S2)、及び7’-ブロモルシフェリン(C11H7BrN2O3S2)、及び4’,5’-ジメチルルシフェリン(C13H12N2O3S2)の分子質量はそれぞれ、406.9116、316.9979、358.9143、及び309.0374であり、これらは、図7に示す予想される化合物の分子質量を示す。
ルシフェリン誘導体である7’-ブロモルシフェリンの合成のための基質は、
- 0.2ミリモルの3-ブロモ-4-ニトロフェノール
- 100ミリモルのHEPESバッファー溶液
- 50マイクロモルの熱安定性デハロゲナーゼ(HadA G513T)
- 2.0マイクロモルのSOD
- 2.0マイクロモルのラッカーゼ
- 2.0マイクロモルのFDH、20ミリモルのギ酸、10.0マイクロモルのNAD+、2.0マイクロモルのC1である実施例1で使用するフラビン依存性レダクターゼ、及び4.0マイクロモルのFADを含む第5のFADH2生成システム
- 2ミリモルのD-システインである。
ルシフェリン誘導体である4’,7’-ジフルオロルシフェリンの合成のための基質は、
- 1.0ミリモルの2,5-ジフルオロ-4-ニトロフェノール
- 100ミリモルのMOPSバッファー溶液
- 50マイクロモルのHadA G513T
- 2.0マイクロモルのSOD
- 2.0マイクロモルのラッカーゼ
- 4.0マイクロモルのGDH、20.0ミリモルのグルコース、10.0マイクロモルのNAD+、2.0マイクロモルのフラビン依存性レダクターゼ(HadX)、4.0マイクロモルのFAD、及び2.0ミリモルのD-システインを含む第4のFADH2生成システムである。
ルシフェリン誘導体である4’,5’-ジメチルルシフェリンの合成のための基質は、
- 0.5ミリモルの2,3-ジメチル-4-ニトロフェノール
- 100ミリモルのリン酸ナトリウムバッファー溶液
- 100マイクロモルの熱安定性デハロゲナーゼ(HadA G513T)
- 5.0マイクロモルのSOD
- 2.0マイクロモルのチロシナーゼ
- NADH及びFADを含み、NADHは、FADからFADH2を産生するための還元剤、かつフラビンレダクターゼ群の基質である、第2のFADH2生成システム
- 5ミリモルのD-システインである。
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