JP5657560B2 - 新規加水分解酵素タンパク質 - Google Patents

新規加水分解酵素タンパク質 Download PDF

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Publication number
JP5657560B2
JP5657560B2 JP2011539573A JP2011539573A JP5657560B2 JP 5657560 B2 JP5657560 B2 JP 5657560B2 JP 2011539573 A JP2011539573 A JP 2011539573A JP 2011539573 A JP2011539573 A JP 2011539573A JP 5657560 B2 JP5657560 B2 JP 5657560B2
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amino acid
seq
acid sequence
protein
hydrolase protein
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JPWO2012029819A1 (ja
Inventor
潤 川端
潤 川端
良磨 三宅
良磨 三宅
久仁子 朝田
久仁子 朝田
良平 加藤
良平 加藤
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API Corp
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API Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
    • C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2011539573A 2010-08-31 2011-08-31 新規加水分解酵素タンパク質 Active JP5657560B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2011539573A JP5657560B2 (ja) 2010-08-31 2011-08-31 新規加水分解酵素タンパク質

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2010194630 2010-08-31
JP2010194630 2010-08-31
PCT/JP2011/069680 WO2012029819A1 (ja) 2010-08-31 2011-08-31 新規加水分解酵素タンパク質
JP2011539573A JP5657560B2 (ja) 2010-08-31 2011-08-31 新規加水分解酵素タンパク質

Publications (2)

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JPWO2012029819A1 JPWO2012029819A1 (ja) 2013-10-31
JP5657560B2 true JP5657560B2 (ja) 2015-01-21

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US (2) US9029107B2 (OSRAM)
EP (1) EP2612913B1 (OSRAM)
JP (1) JP5657560B2 (OSRAM)
CN (1) CN102834515B (OSRAM)
IN (1) IN2013CN00757A (OSRAM)
SG (1) SG187652A1 (OSRAM)
WO (1) WO2012029819A1 (OSRAM)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019189724A1 (ja) 2018-03-30 2019-10-03 株式会社エーピーアイ コーポレーション 新規加水分解酵素及びそれを利用した（１ｓ，２ｓ）－１－アルコキシカルボニル－２－ビニルシクロプロパンカルボン酸の製造方法
KR20190138651A (ko) * 2017-04-07 2019-12-13 듀폰 뉴트리션 바이오사이언시즈 에이피에스 p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5657560B2 (ja) 2010-08-31 2015-01-21 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質
JP6158168B2 (ja) * 2012-03-14 2017-07-05 積水メディカル株式会社 光学活性２−ビニルシクロプロパン−１，１−ジカルボン酸エステルの製造法
IN2015DN02424A (OSRAM) 2012-12-20 2015-09-04 Api Corp
US9540622B2 (en) 2013-01-18 2017-01-10 Codexis, Inc. Engineered biocatalysts useful for carbapenem synthesis
WO2014129459A1 (ja) 2013-02-19 2014-08-28 株式会社エーピーアイ コーポレーション Ｌ－リジン水酸化酵素およびそれを利用したヒドロキシ－ｌ－リジンの製造法およびヒドロキシ－ｌ－ピペコリン酸の製造法
TWI758313B (zh) * 2016-10-12 2022-03-21 美商陶氏農業科學公司 一種用於製備(1r,3r)-及(1s,3s)-2,2-二鹵基-3-(經取代之苯基)環丙烷甲酸的方法
CN110283804A (zh) * 2019-07-18 2019-09-27 江苏省农业科学院 酯酶基因及编码蛋白与应用
EP4067497A4 (en) * 2019-11-29 2024-03-13 MicroBiopharm Japan Co., Ltd. Method for enzymatically producing maytansinol
CN114480343B (zh) * 2020-10-27 2023-07-04 湖北大学 具提升活性的pet水解酶
CN115109765B (zh) * 2022-06-24 2023-10-13 浙江工业大学 酯酶pnbA-BS突变体及其在制备l-薄荷醇中的应用
CN116555222A (zh) * 2023-05-17 2023-08-08 南京师范大学 2-羟乙基对苯二甲酸二酯水解酶突变体及编码基因、重组载体、重组菌株、酶制剂和应用
CN116987653A (zh) * 2023-08-03 2023-11-03 南京工业大学 一种表达牛乳铁蛋白的解淀粉芽孢杆菌及其构建方法与应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006269A1 (fr) * 1986-04-16 1987-10-22 Sumitomo Chemical Company, Limited Procede pour preparer des acides cyclopropanecarboxyliques optiquement actifs
JP2009502184A (ja) * 2005-08-05 2009-01-29 ヘンケル・アクチェンゲゼルシャフト・ウント・コムパニー・コマンディットゲゼルシャフト・アウフ・アクチェン プラスチック分離へのエステラーゼの利用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57134500A (en) 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
US5750380A (en) 1981-06-30 1998-05-12 City Of Hope Research Institute DNA polymerase mediated synthesis of double stranded nucleic acids
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
JPS5867679A (ja) 1981-09-30 1983-04-22 アメリカン・サイアナミド・カンパニ− イソシアン酸を三量化してシアヌル酸をつくる方法
JPS5877895A (ja) 1981-11-02 1983-05-11 Ajinomoto Co Inc プラスミドｐｈｍ１５１９
JPS58192900A (ja) 1982-05-04 1983-11-10 Ajinomoto Co Inc 複合プラスミド
US5180671A (en) 1986-04-16 1993-01-19 Sumitomo Chemical Company, Limited Method for producing optically active cyclopropane carboxylic acid
JPH0679557B2 (ja) 1986-04-16 1994-10-12 住友化学工業株式会社 光学活性なシス−シクロプロパンカルボン酸の製造方法
JPH01191686A (ja) 1988-01-26 1989-08-01 Mitsubishi Petrochem Co Ltd 複合プラスミド
JP2522538B2 (ja) 1989-01-31 1996-08-07 株式会社紀文食品 耐熱性の高いヌクレア―ゼ画分の製造方法
JP2973446B2 (ja) 1990-01-11 1999-11-08 三菱化学株式会社 新規プラスミドベクター
US5468632A (en) * 1991-12-20 1995-11-21 Eli Lilly And Company Recombinant DNA compounds and expression vectors encoding para-nitrobenzyl esterase activity from bacillus
US5741691A (en) 1996-01-23 1998-04-21 California Institute Of Technology Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US5945325A (en) * 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
JP2005034025A (ja) 2003-07-18 2005-02-10 Mitsubishi Pharma Corp 大腸菌での異種遺伝子発現を促進させるプラスミドベクター、組換え大腸菌、該組換え大腸菌を用いた化学物質製造法及び組換えタンパク質の製造法
JP4763462B2 (ja) 2004-02-04 2011-08-31 株式会社エーピーアイ コーポレーション 光学活性を有するアルコール及びカルボン酸の製造方法
CN102834370B (zh) * 2010-02-16 2016-11-23 株式会社Api 1-氨基-1-烷氧基羰基-2-乙烯基环丙烷的制备方法
JP5657560B2 (ja) 2010-08-31 2015-01-21 株式会社エーピーアイ コーポレーション 新規加水分解酵素タンパク質

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987006269A1 (fr) * 1986-04-16 1987-10-22 Sumitomo Chemical Company, Limited Procede pour preparer des acides cyclopropanecarboxyliques optiquement actifs
JP2009502184A (ja) * 2005-08-05 2009-01-29 ヘンケル・アクチェンゲゼルシャフト・ウント・コムパニー・コマンディットゲゼルシャフト・アウフ・アクチェン プラスチック分離へのエステラーゼの利用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JPN6011062139; Ｇｅｎｅ ｖｏｌ．１５１，　ｎｏ．１-２, 1994, ｐｐ．３７-４３ *
JPN6011062141; Ｓｙｎｔｈ．　Ｃｏｍｍｕｎ． ｖｏｌ．２４，　ｎｏ．２０, 1994, ｐｐ．２８７３-２８７６ *
JPN6011062143; Ｍａｃｒｏｍｏｌ．　Ｒａｐｉｄ　Ｃｏｍｍｕｎ． ｖｏｌ．２２，　ｎｏ．１６, 2001, ｐｐ．１３５０-１３５３ *
JPN6011062145; Ｊ．　Ｏｒｇ．　Ｃｈｅｍ． ｖｏｌ．７０，　ｎｏ．１５, 2005, ｐｐ．５８６９-５８７９ *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190138651A (ko) * 2017-04-07 2019-12-13 듀폰 뉴트리션 바이오사이언시즈 에이피에스 p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포
KR102533803B1 (ko) * 2017-04-07 2023-05-18 듀폰 뉴트리션 바이오사이언시즈 에이피에스 p-니트로벤질에스터라제 부활성의 부재 하에 β-갈락토시다제 및 락타제를 생산하는 바실러스 숙주 세포
WO2019189724A1 (ja) 2018-03-30 2019-10-03 株式会社エーピーアイ コーポレーション 新規加水分解酵素及びそれを利用した（１ｓ，２ｓ）－１－アルコキシカルボニル－２－ビニルシクロプロパンカルボン酸の製造方法
US11572551B2 (en) 2018-03-30 2023-02-07 Api Corporation Hydrolase and method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid using same
US12258593B2 (en) 2018-03-30 2025-03-25 Ube Corporation Hydrolase and method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid using same

Also Published As

Publication number Publication date
EP2612913B1 (en) 2016-07-27
US9334509B2 (en) 2016-05-10
SG187652A1 (en) 2013-03-28
US9029107B2 (en) 2015-05-12
JPWO2012029819A1 (ja) 2013-10-31
US20130130338A1 (en) 2013-05-23
CN102834515B (zh) 2016-08-03
EP2612913A4 (en) 2014-03-26
CN102834515A (zh) 2012-12-19
IN2013CN00757A (OSRAM) 2015-04-24
US20150252393A1 (en) 2015-09-10
WO2012029819A1 (ja) 2012-03-08
EP2612913A1 (en) 2013-07-10

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