IN2013CN00757A - - Google Patents

Info

Publication number
IN2013CN00757A
IN2013CN00757A IN757CHN2013A IN2013CN00757A IN 2013CN00757 A IN2013CN00757 A IN 2013CN00757A IN 757CHN2013 A IN757CHN2013 A IN 757CHN2013A IN 2013CN00757 A IN2013CN00757 A IN 2013CN00757A
Authority
IN
India
Prior art keywords
seq
vinylcyclopropane
sequence represented
protein
amino acid
Prior art date
Application number
Inventor
Hiroshi Kawabata
Ryoma Miyake
Kuniko Asada
Ryouhei Katou
Original Assignee
Api Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Api Corp filed Critical Api Corp
Publication of IN2013CN00757A publication Critical patent/IN2013CN00757A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
    • C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The purpose of the present invention is to provide a novel hydrolase which can be used for efficiently producing a (1S 2S) 1 alkoxycarbonyl 2 vinylcyclopropanecarboxylic acid which is a compound useful as an intermediate for the synthesis of a therapeutic agent for hepatitis C through the hydrolysis of a dialkyl 2 vinylcyclopropane 1 1 dicarboxylic acid with an enzyme. The present invention provides a hydrolase protein which comprises an amino acid sequence represented by any one of SEQ ID NO:2 to SEQ ID NO:5 and which has an activity of catalyzing the reaction of the conversion of diethyl 2 vinylcyclopropane 1 1 dicarboxylic acid to (1S 2S) 1 ethoxycarbonyl 2 vinylcyclopropanecarboxylic acid with higher selectivity than that of a protein comprising the amino acid sequence represented by SEQ ID NO:1.
IN757CHN2013 2010-08-31 2011-08-31 IN2013CN00757A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010194630 2010-08-31
PCT/JP2011/069680 WO2012029819A1 (en) 2010-08-31 2011-08-31 Novel hydrolase protein

Publications (1)

Publication Number Publication Date
IN2013CN00757A true IN2013CN00757A (en) 2015-04-24

Family

ID=45772898

Family Applications (1)

Application Number Title Priority Date Filing Date
IN757CHN2013 IN2013CN00757A (en) 2010-08-31 2011-08-31

Country Status (7)

Country Link
US (2) US9029107B2 (en)
EP (1) EP2612913B1 (en)
JP (1) JP5657560B2 (en)
CN (1) CN102834515B (en)
IN (1) IN2013CN00757A (en)
SG (1) SG187652A1 (en)
WO (1) WO2012029819A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5657560B2 (en) 2010-08-31 2015-01-21 株式会社エーピーアイ コーポレーション Novel hydrolase protein
WO2013137330A1 (en) * 2012-03-14 2013-09-19 積水メディカル株式会社 Method for producing optically active 2-vinylcyclopropane-1,1-dicarboxylic acid ester
JP6370712B2 (en) 2012-12-20 2018-08-08 株式会社エーピーアイ コーポレーション Method for producing cis-5-hydroxy-2-piperidinecarboxylic acid derivative and method for purifying cis-5-hydroxy-2-piperidinecarboxylic acid
EP2946006B1 (en) * 2013-01-18 2019-04-10 Codexis, Inc. Engineered biocatalysts useful for carbapenem synthesis
CN104685061B (en) 2013-02-19 2018-05-01 株式会社Api L-lysine hydroxylase and the autofrettage of hydroxy-L-lysine and the autofrettage of hydroxyl-dl pipecolinic acid that make use of the L-lysine hydroxylase
TWI758313B (en) * 2016-10-12 2022-03-21 美商陶氏農業科學公司 Process for the preparation of (1r,3r)- and (1s,3s)-2,2-dihalo-3-(substituted phenyl)cyclopropanecarboxylic acids
US11479763B2 (en) * 2017-04-07 2022-10-25 Dupont Nutrition Biosciences Aps Bacillus host cells producing β-galactosidases and lactases in the absence of p-nitrobenzylesterase side activity
EP3778898A4 (en) * 2018-03-30 2022-02-09 API Corporation Novel hydrolase and method for producing (1s,2s)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid using same
CN110283804A (en) * 2019-07-18 2019-09-27 江苏省农业科学院 Esterase gene and coding albumen and application
WO2021107133A1 (en) * 2019-11-29 2021-06-03 日本マイクロバイオファーマ株式会社 Method for enzymatically producing maytansinol
CN114480343B (en) * 2020-10-27 2023-07-04 湖北大学 PET hydrolase with enhanced activity
CN115109765B (en) * 2022-06-24 2023-10-13 浙江工业大学 Esterase pnbA-BS mutant and application thereof in preparation of l-menthol

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57134500A (en) 1981-02-12 1982-08-19 Kyowa Hakko Kogyo Co Ltd Plasmid pcg1
JPS57183799A (en) 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
US5750380A (en) 1981-06-30 1998-05-12 City Of Hope Research Institute DNA polymerase mediated synthesis of double stranded nucleic acids
JPS5835197A (en) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd Plamid pcg 2
JPS5867679A (en) 1981-09-30 1983-04-22 アメリカン・サイアナミド・カンパニ− Manufacture of cyanuric acid by trimerizing isocyanic acid
JPS5877895A (en) 1981-11-02 1983-05-11 Ajinomoto Co Inc Plasmid phm1519
JPS58192900A (en) 1982-05-04 1983-11-10 Ajinomoto Co Inc Complex plasmid
JPH0679557B2 (en) 1986-04-16 1994-10-12 住友化学工業株式会社 Process for producing optically active cis-cyclopropanecarboxylic acid
US5180671A (en) 1986-04-16 1993-01-19 Sumitomo Chemical Company, Limited Method for producing optically active cyclopropane carboxylic acid
DE3752086T2 (en) * 1986-04-16 1998-02-26 Sumitomo Chemical Co Process for the production of optically active cyclopropanecarboxylic acid
JPH01191686A (en) 1988-01-26 1989-08-01 Mitsubishi Petrochem Co Ltd Composite plasmid
JP2522538B2 (en) 1989-01-31 1996-08-07 株式会社紀文食品 A method for producing a highly heat-resistant nucleasease fraction
JP2973446B2 (en) 1990-01-11 1999-11-08 三菱化学株式会社 New plasmid vector
US5468632A (en) * 1991-12-20 1995-11-21 Eli Lilly And Company Recombinant DNA compounds and expression vectors encoding para-nitrobenzyl esterase activity from bacillus
US5741691A (en) 1996-01-23 1998-04-21 California Institute Of Technology Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media
DE19736591A1 (en) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Preparing long nucleic acid polymers from linkable oligonucleotides
US5945325A (en) * 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
JP2005034025A (en) 2003-07-18 2005-02-10 Mitsubishi Pharma Corp Plasmid vector for promoting expression of foreign gene in escherichia coli, recombinant escherichia coli, method for producing chemical substance with recombinant escherichia coli, and method for producing recombinant protein
EP2270178A3 (en) 2004-02-04 2011-02-23 API Corporation Method for producing alcohol and carboxylic acid having optical activity
DE102005037659A1 (en) 2005-08-05 2007-02-22 Henkel Kgaa Use of esterases for splitting plastics
US9061991B2 (en) * 2010-02-16 2015-06-23 Api Corporation Method for producing 1-amino-1-alkoxycarbonyl-2-vinylcyclopropane
JP5657560B2 (en) 2010-08-31 2015-01-21 株式会社エーピーアイ コーポレーション Novel hydrolase protein

Also Published As

Publication number Publication date
CN102834515A (en) 2012-12-19
US9029107B2 (en) 2015-05-12
EP2612913B1 (en) 2016-07-27
JP5657560B2 (en) 2015-01-21
US9334509B2 (en) 2016-05-10
SG187652A1 (en) 2013-03-28
US20130130338A1 (en) 2013-05-23
EP2612913A4 (en) 2014-03-26
EP2612913A1 (en) 2013-07-10
CN102834515B (en) 2016-08-03
US20150252393A1 (en) 2015-09-10
JPWO2012029819A1 (en) 2013-10-31
WO2012029819A1 (en) 2012-03-08

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