IN2013CN00757A - - Google Patents
Info
- Publication number
- IN2013CN00757A IN2013CN00757A IN757CHN2013A IN2013CN00757A IN 2013CN00757 A IN2013CN00757 A IN 2013CN00757A IN 757CHN2013 A IN757CHN2013 A IN 757CHN2013A IN 2013CN00757 A IN2013CN00757 A IN 2013CN00757A
- Authority
- IN
- India
- Prior art keywords
- seq
- vinylcyclopropane
- sequence represented
- protein
- amino acid
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
- C12P41/003—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions
- C12P41/005—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by ester formation, lactone formation or the inverse reactions by esterification of carboxylic acid groups in the enantiomers or the inverse reaction
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Abstract
The purpose of the present invention is to provide a novel hydrolase which can be used for efficiently producing a (1S 2S) 1 alkoxycarbonyl 2 vinylcyclopropanecarboxylic acid which is a compound useful as an intermediate for the synthesis of a therapeutic agent for hepatitis C through the hydrolysis of a dialkyl 2 vinylcyclopropane 1 1 dicarboxylic acid with an enzyme. The present invention provides a hydrolase protein which comprises an amino acid sequence represented by any one of SEQ ID NO:2 to SEQ ID NO:5 and which has an activity of catalyzing the reaction of the conversion of diethyl 2 vinylcyclopropane 1 1 dicarboxylic acid to (1S 2S) 1 ethoxycarbonyl 2 vinylcyclopropanecarboxylic acid with higher selectivity than that of a protein comprising the amino acid sequence represented by SEQ ID NO:1.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010194630 | 2010-08-31 | ||
PCT/JP2011/069680 WO2012029819A1 (en) | 2010-08-31 | 2011-08-31 | Novel hydrolase protein |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2013CN00757A true IN2013CN00757A (en) | 2015-04-24 |
Family
ID=45772898
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN757CHN2013 IN2013CN00757A (en) | 2010-08-31 | 2011-08-31 |
Country Status (7)
Country | Link |
---|---|
US (2) | US9029107B2 (en) |
EP (1) | EP2612913B1 (en) |
JP (1) | JP5657560B2 (en) |
CN (1) | CN102834515B (en) |
IN (1) | IN2013CN00757A (en) |
SG (1) | SG187652A1 (en) |
WO (1) | WO2012029819A1 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2612913B1 (en) | 2010-08-31 | 2016-07-27 | API Corporation | Novel hydrolase protein |
JP6158168B2 (en) * | 2012-03-14 | 2017-07-05 | 積水メディカル株式会社 | Process for producing optically active 2-vinylcyclopropane-1,1-dicarboxylic acid ester |
SG10201705091YA (en) | 2012-12-20 | 2017-07-28 | Api Corp | METHOD FOR PRODUCING cis-5-HYDROXY-2-PIPERIDINECARBOXYLIC ACID DERIVATIVE, AND METHOD FOR PURIFYING cis-5-HYDROXY-2-PIPERIDINECARBOXYLIC ACID |
JP6486835B2 (en) * | 2013-01-18 | 2019-03-20 | コデクシス, インコーポレイテッド | Useful biocatalysts for carbapenem synthesis |
WO2014129459A1 (en) | 2013-02-19 | 2014-08-28 | 株式会社エーピーアイ コーポレーション | Production method for l-lysine hydroxylase and hydroxy-l-lysine using same, and production method for hydroxy-l-pipecolic acid |
TWI758313B (en) * | 2016-10-12 | 2022-03-21 | 美商陶氏農業科學公司 | Process for the preparation of (1r,3r)- and (1s,3s)-2,2-dihalo-3-(substituted phenyl)cyclopropanecarboxylic acids |
JP7065114B2 (en) * | 2017-04-07 | 2022-05-11 | デュポン ニュートリション バイオサイエンシス エーピーエス | Bacillus host cell producing β-galactosidase and lactase in the absence of p-nitrobenzyl esterase side activity |
EP3778898A4 (en) * | 2018-03-30 | 2022-02-09 | API Corporation | Novel hydrolase and method for producing (1s,2s)-1-alkoxycarbonyl-2-vinylcyclopropane carboxylic acid using same |
CN110283804A (en) * | 2019-07-18 | 2019-09-27 | 江苏省农业科学院 | Esterase gene and coding albumen and application |
EP4067497A4 (en) * | 2019-11-29 | 2024-03-13 | Microbiopharm Japan Co Ltd | Method for enzymatically producing maytansinol |
CN114480343B (en) * | 2020-10-27 | 2023-07-04 | 湖北大学 | PET hydrolase with enhanced activity |
CN115109765B (en) * | 2022-06-24 | 2023-10-13 | 浙江工业大学 | Esterase pnbA-BS mutant and application thereof in preparation of l-menthol |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS57134500A (en) | 1981-02-12 | 1982-08-19 | Kyowa Hakko Kogyo Co Ltd | Plasmid pcg1 |
JPS57183799A (en) | 1981-04-17 | 1982-11-12 | Kyowa Hakko Kogyo Co Ltd | Novel plasmid |
US5750380A (en) | 1981-06-30 | 1998-05-12 | City Of Hope Research Institute | DNA polymerase mediated synthesis of double stranded nucleic acids |
JPS5835197A (en) | 1981-08-26 | 1983-03-01 | Kyowa Hakko Kogyo Co Ltd | Plamid pcg 2 |
JPS5867679A (en) | 1981-09-30 | 1983-04-22 | アメリカン・サイアナミド・カンパニ− | Manufacture of cyanuric acid by trimerizing isocyanic acid |
JPS5877895A (en) | 1981-11-02 | 1983-05-11 | Ajinomoto Co Inc | Plasmid phm1519 |
JPS58192900A (en) | 1982-05-04 | 1983-11-10 | Ajinomoto Co Inc | Complex plasmid |
US5180671A (en) | 1986-04-16 | 1993-01-19 | Sumitomo Chemical Company, Limited | Method for producing optically active cyclopropane carboxylic acid |
JPH0679557B2 (en) | 1986-04-16 | 1994-10-12 | 住友化学工業株式会社 | Process for producing optically active cis-cyclopropanecarboxylic acid |
DE3752086T2 (en) | 1986-04-16 | 1998-02-26 | Sumitomo Chemical Co | Process for the production of optically active cyclopropanecarboxylic acid |
JPH01191686A (en) | 1988-01-26 | 1989-08-01 | Mitsubishi Petrochem Co Ltd | Composite plasmid |
JP2522538B2 (en) | 1989-01-31 | 1996-08-07 | 株式会社紀文食品 | A method for producing a highly heat-resistant nucleasease fraction |
JP2973446B2 (en) | 1990-01-11 | 1999-11-08 | 三菱化学株式会社 | New plasmid vector |
US5468632A (en) * | 1991-12-20 | 1995-11-21 | Eli Lilly And Company | Recombinant DNA compounds and expression vectors encoding para-nitrobenzyl esterase activity from bacillus |
US5741691A (en) * | 1996-01-23 | 1998-04-21 | California Institute Of Technology | Para-nitrobenzyl esterases with enhanced activity in aqueous and nonaqueous media |
DE19736591A1 (en) | 1997-08-22 | 1999-02-25 | Peter Prof Dr Hegemann | Preparing long nucleic acid polymers from linkable oligonucleotides |
US5945325A (en) * | 1998-04-20 | 1999-08-31 | California Institute Of Technology | Thermally stable para-nitrobenzyl esterases |
JP2005034025A (en) | 2003-07-18 | 2005-02-10 | Mitsubishi Pharma Corp | Plasmid vector for promoting expression of foreign gene in escherichia coli, recombinant escherichia coli, method for producing chemical substance with recombinant escherichia coli, and method for producing recombinant protein |
WO2005075651A1 (en) | 2004-02-04 | 2005-08-18 | Api Corporation | Process for producing optically active alcohol and carboxylic acid |
DE102005037659A1 (en) * | 2005-08-05 | 2007-02-22 | Henkel Kgaa | Use of esterases for splitting plastics |
US9061991B2 (en) * | 2010-02-16 | 2015-06-23 | Api Corporation | Method for producing 1-amino-1-alkoxycarbonyl-2-vinylcyclopropane |
EP2612913B1 (en) | 2010-08-31 | 2016-07-27 | API Corporation | Novel hydrolase protein |
-
2011
- 2011-08-31 EP EP11821834.6A patent/EP2612913B1/en active Active
- 2011-08-31 IN IN757CHN2013 patent/IN2013CN00757A/en unknown
- 2011-08-31 JP JP2011539573A patent/JP5657560B2/en active Active
- 2011-08-31 WO PCT/JP2011/069680 patent/WO2012029819A1/en active Application Filing
- 2011-08-31 CN CN201180009868.2A patent/CN102834515B/en active Active
- 2011-08-31 SG SG2013007422A patent/SG187652A1/en unknown
- 2011-08-31 US US13/813,047 patent/US9029107B2/en active Active
-
2015
- 2015-03-04 US US14/637,429 patent/US9334509B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP2612913B1 (en) | 2016-07-27 |
CN102834515A (en) | 2012-12-19 |
JP5657560B2 (en) | 2015-01-21 |
US20150252393A1 (en) | 2015-09-10 |
WO2012029819A1 (en) | 2012-03-08 |
US20130130338A1 (en) | 2013-05-23 |
EP2612913A1 (en) | 2013-07-10 |
US9334509B2 (en) | 2016-05-10 |
JPWO2012029819A1 (en) | 2013-10-31 |
SG187652A1 (en) | 2013-03-28 |
CN102834515B (en) | 2016-08-03 |
EP2612913A4 (en) | 2014-03-26 |
US9029107B2 (en) | 2015-05-12 |
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