IN2014KN00866A - - Google Patents

Info

Publication number
IN2014KN00866A
IN2014KN00866A IN866KON2014A IN2014KN00866A IN 2014KN00866 A IN2014KN00866 A IN 2014KN00866A IN 866KON2014 A IN866KON2014 A IN 866KON2014A IN 2014KN00866 A IN2014KN00866 A IN 2014KN00866A
Authority
IN
India
Prior art keywords
amino acid
seq
acid sequence
hydantoinase
catalytic activity
Prior art date
Application number
Inventor
Steffen Osswald
Heiko Schuster
Jürgen Roos
Andreas Karau
Ulrich Schwaneberg
Ronny Martinez
Hemanshu Mundhada
Ursula Holter
Original Assignee
Evonik Industries Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Industries Ag filed Critical Evonik Industries Ag
Publication of IN2014KN00866A publication Critical patent/IN2014KN00866A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/227—Tryptophan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C12P41/009—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures by reactions involving hydantoins or carbamoylamino compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02002—Dihydropyrimidinase (3.5.2.2), i.e. hydantoinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a hydantoinase having an amino acid sequence selected from (i) or (ii) with (i) amino acid sequence selected from SEQ ID NO: 6 20 and SEQ ID NO: 73 119 (ii) amino acid sequence wherein in the amino acid sequence of SEQ ID NO: 6 20 and SEQ ID NO: 73 119 1 to 75 amino acid residues have been substituted deleted inserted and/or added and wherein further the catalytic activity of the hydantoinase is higher by a factor of at least 1.2 than the catalytic activity of the hydantoinase having amino acid sequence SEQ ID NO: 1 The present invention further relates to a process for preparing amino acids wherein said hydantoinase is used.
IN866KON2014 2011-11-16 2012-11-16 IN2014KN00866A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11189395 2011-11-16
PCT/EP2012/072891 WO2013072486A1 (en) 2011-11-16 2012-11-16 Mutants of hydantoinase

Publications (1)

Publication Number Publication Date
IN2014KN00866A true IN2014KN00866A (en) 2015-10-02

Family

ID=47189946

Family Applications (1)

Application Number Title Priority Date Filing Date
IN866KON2014 IN2014KN00866A (en) 2011-11-16 2012-11-16

Country Status (12)

Country Link
US (2) US9957536B2 (en)
EP (1) EP2780451B1 (en)
JP (1) JP6279478B2 (en)
KR (1) KR102027201B1 (en)
CN (2) CN104053772A (en)
BR (1) BR112014011664A2 (en)
CA (1) CA2856127C (en)
DK (1) DK2780451T3 (en)
ES (1) ES2652387T3 (en)
IN (1) IN2014KN00866A (en)
PL (1) PL2780451T3 (en)
WO (1) WO2013072486A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175929A (en) * 2020-10-10 2021-01-05 山西大学 Recombinant hydantoin racemase engineering bacteria and construction method and application thereof
EP4105335A1 (en) 2021-06-16 2022-12-21 Evonik Operations GmbH Enzymatic method for the production of l-glufosinate p-alkyl esters
CN114686465B (en) * 2021-11-21 2024-03-22 宁波酶赛生物工程有限公司 Synthesis method of hydrolase and (R) - (-) -3- (carbamoylmethyl) -5-methylhexanoic acid
CA3245643A1 (en) 2022-03-14 2023-09-21 Evonik Operations Gmbh Enzymatic method for the production of l-glufosinate p-esters
WO2023222227A1 (en) 2022-05-19 2023-11-23 Evonik Operations Gmbh Enzymatic method for producing l-glufosinate
WO2023222226A1 (en) 2022-05-19 2023-11-23 Evonik Operations Gmbh Enzymatic method for producing l-glufosinate
WO2023232225A1 (en) 2022-05-31 2023-12-07 Evonik Operations Gmbh Enzymatic method for the diastereoselective production of l-glufosinate p-esters
CN120699948A (en) * 2025-07-15 2025-09-26 上海奥萝拉医药科技有限公司 Hydantoinase mutant and its application

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5391189A (en) * 1976-12-30 1978-08-10 Kanegafuchi Chem Ind Co Ltd Preparation of d-n-carbamoyl-alpha-amino acids
JPS6225990A (en) * 1986-07-19 1987-02-03 Kanegafuchi Chem Ind Co Ltd Production of d-alpha-amino acid
DE4316928C2 (en) 1993-05-19 1995-03-16 Degussa New microorganisms, their use and process for the production of L-alpha-amino acids
US6524837B1 (en) * 1999-03-29 2003-02-25 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
DE10114999A1 (en) 2001-03-26 2002-10-10 Degussa D-carbamoylase from Arthrobacter crystallopoietes DSM 20117
DE10155928A1 (en) 2001-11-15 2003-06-12 Degussa recA negative and RhaB negative microorganism
CN1260347C (en) * 2002-05-13 2006-06-21 中国人民解放军军事医学科学院生物工程研究所 Hydantoin enzyme series and encoded gene and production bacteria thereof
DE10251184A1 (en) * 2002-11-04 2004-05-13 Degussa Ag Recombinant microorganism for D-amino acid production, useful as intermediates for pharmaceuticals, has genes involved in D-amino acid degradation inactivated, for improved yield
JP2007189949A (en) * 2006-01-19 2007-08-02 Kaneka Corp New hydantoinase and method for producing optically active amino acid derivative

Also Published As

Publication number Publication date
CA2856127A1 (en) 2013-05-23
US9957536B2 (en) 2018-05-01
US20180237814A1 (en) 2018-08-23
WO2013072486A1 (en) 2013-05-23
PL2780451T3 (en) 2018-03-30
US20150056667A1 (en) 2015-02-26
DK2780451T3 (en) 2018-01-02
KR102027201B1 (en) 2019-10-01
EP2780451A1 (en) 2014-09-24
CN109468304A (en) 2019-03-15
JP2014533497A (en) 2014-12-15
BR112014011664A2 (en) 2017-05-09
CN104053772A (en) 2014-09-17
CN109468304B (en) 2022-04-19
JP6279478B2 (en) 2018-02-14
KR20140092835A (en) 2014-07-24
EP2780451B1 (en) 2017-09-20
US10822627B2 (en) 2020-11-03
CA2856127C (en) 2021-04-06
ES2652387T3 (en) 2018-02-01

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