JP5376478B2 - 効率的な人工多能性幹細胞の樹立方法 - Google Patents

効率的な人工多能性幹細胞の樹立方法 Download PDF

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JP5376478B2
JP5376478B2 JP2012523493A JP2012523493A JP5376478B2 JP 5376478 B2 JP5376478 B2 JP 5376478B2 JP 2012523493 A JP2012523493 A JP 2012523493A JP 2012523493 A JP2012523493 A JP 2012523493A JP 5376478 B2 JP5376478 B2 JP 5376478B2
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圭介 沖田
誠人 中川
伸弥 山中
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Kyoto University NUC
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JP2012523493A 2009-08-07 2010-08-06 効率的な人工多能性幹細胞の樹立方法 Active JP5376478B2 (ja)

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US23240209P 2009-08-07 2009-08-07
US61/232,402 2009-08-07
US30730610P 2010-02-23 2010-02-23
US61/307,306 2010-02-23
PCT/JP2010/063733 WO2011016588A1 (en) 2009-08-07 2010-08-06 Method of efficiently establishing induced pluripotent stem cells

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JP2013501505A5 JP2013501505A5 (OSRAM) 2013-03-21
JP5376478B2 true JP5376478B2 (ja) 2013-12-25

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US (2) US8900871B2 (OSRAM)
EP (1) EP2462225B1 (OSRAM)
JP (1) JP5376478B2 (OSRAM)
KR (1) KR101774206B1 (OSRAM)
CN (1) CN102625837B (OSRAM)
AU (1) AU2010279913B2 (OSRAM)
CA (1) CA2770412C (OSRAM)
ES (1) ES2726766T3 (OSRAM)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12195764B2 (en) 2020-12-29 2025-01-14 I Peace, Inc. Method for culturing cells into which reprogramming factor is introduced

Families Citing this family (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2836764T3 (es) 2005-08-03 2021-06-28 Astellas Inst For Regenerative Medicine Métodos mejorados de reprogramación de células somáticas animales
US9534205B2 (en) 2008-03-17 2017-01-03 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
WO2009115295A1 (en) * 2008-03-17 2009-09-24 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
JP5340265B2 (ja) 2008-06-27 2013-11-13 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2010013845A1 (en) * 2008-07-30 2010-02-04 Kyoto University Method of efficiently establishing induced pluripotent stem cells
JP6093110B2 (ja) 2008-12-17 2017-03-08 ザ スクリプス リサーチ インスティテュート 幹細胞の作製と維持
CA2753845C (en) 2009-02-27 2019-10-29 Kyoto University Nuclear reprogramming substance comprising glis1
SG10201608797WA (en) 2009-08-07 2016-12-29 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
JP5773393B2 (ja) 2009-09-24 2015-09-02 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
BR112012008848A2 (pt) 2009-10-16 2019-09-24 Scripps Research Inst composição, e, método in vitro ou ex vivo para induzir células de mamífero não-pluripotente em células tronco pluripotentes induzidas
JP5898086B2 (ja) 2009-11-04 2016-04-06 セルラー ダイナミクス インターナショナル， インコーポレイテッド 化学物質を用いるエピソームリプログラミング
KR101857302B1 (ko) 2010-02-16 2018-05-11 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적 확립 방법
WO2011119942A1 (en) * 2010-03-25 2011-09-29 Vistagen Therapeutics, Inc. Induction of ips cells using transient episomal vectors
ES2893699T3 (es) 2010-03-31 2022-02-09 Scripps Research Inst Reprogramación de células
EP2580320B1 (en) 2010-06-14 2018-08-01 The Scripps Research Institute Reprogramming of cells to a new fate
CN103003416B (zh) * 2010-06-15 2016-11-16 细胞动力学国际有限公司 从小体积的外周血产生诱导性多潜能干细胞
JP5794588B2 (ja) 2010-09-14 2015-10-14 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
EP2627342B1 (en) * 2010-10-14 2016-08-24 University Of Central Florida Research Foundation, Inc. Cardiac induced pluripotent stem cells and methods of use in repair and regeneration of myocardium
KR102482184B1 (ko) 2010-12-22 2022-12-28 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
CN103562376B (zh) * 2011-04-08 2017-12-29 国家医疗保健研究所 复壮细胞的方法
WO2012137970A1 (ja) 2011-04-08 2012-10-11 国立大学法人大阪大学 改変ラミニンおよびその利用
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
WO2013022022A1 (ja) * 2011-08-08 2013-02-14 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2013074916A1 (en) 2011-11-18 2013-05-23 Board Of Regents, The University Of Texas System Car+ t cells genetically modified to eliminate expression of t- cell receptor and/or hla
US20130157368A1 (en) * 2011-12-20 2013-06-20 Advanced Technologies And Regenerative Medicine, Llc Induced pluripotent stem cells prepared from human kidney-derived cells
US20130157365A1 (en) * 2011-12-20 2013-06-20 Advanced Technologies And Regenerative Medicine, Llc Induced pluripotent stem cells from human umbilical cord tissue-derived cells
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10119150B2 (en) * 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
CA2874259C (en) * 2012-05-23 2021-02-09 Kyoto University Highly efficient method for establishing induced pluripotent stem cell
CN102965393B (zh) * 2012-11-06 2015-08-26 上海交通大学 人诱导多能干细胞的制备方法及其用途
US20150368713A1 (en) 2013-02-01 2015-12-24 THE UNITED STATES OF AMERICAN, as represented by the Secretary, Department of Health and Human Serv METHOD FOR GENERATING RETINAL PIGMENT EPITHELIUM (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCs)
WO2014192312A1 (ja) * 2013-05-31 2014-12-04 国立大学法人 東京医科歯科大学 正常な内向きのカリウム電流特性を有するｉＰＳ細胞由来心筋モデル細胞
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
CN103571794B (zh) * 2013-07-19 2016-01-20 中国医学科学院血液病医院（血液学研究所） 一种抑制PUMA功能改善iPS细胞建立功效的方法
CN103725710B (zh) * 2013-12-25 2015-10-28 北京济福霖生物技术有限公司 一种可自我删除游离载体及其应用
CN103740756B (zh) * 2013-12-25 2015-08-05 中国农业大学 一种可调控删除的非病毒游离载体及其构建方法
CN103740757B (zh) * 2014-01-21 2016-04-20 中国科学院生物物理研究所 一种利用重编程制备猪神经干细胞的方法
CN104830906B (zh) * 2014-02-12 2018-09-04 北京维通达生物技术有限公司 一种重编程获得功能性人肝脏实质细胞的方法
KR20240091064A (ko) 2014-03-04 2024-06-21 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
US10927384B2 (en) 2014-04-09 2021-02-23 Dna Twopointo Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
CN106459918A (zh) * 2014-04-24 2017-02-22 得克萨斯州大学系统董事会 施加诱导多能干细胞产生过继细胞疗法产品
EP3152295B1 (en) * 2014-06-05 2020-07-29 Cedars-Sinai Medical Center A novel and efficient method for reprogramming immortalized lymphoblastoid cell lines to induced pluripotent stem cells
WO2016117139A1 (ja) * 2015-01-20 2016-07-28 タカラバイオ株式会社 神経系細胞の製造方法
WO2016120493A1 (en) * 2015-01-30 2016-08-04 Centre National De La Recherche Scientifique (Cnrs) Reprogramming method for producing induced pluripotent stem cells (ipsc)
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
EP3332020B1 (en) 2015-08-07 2023-12-20 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Method of determining cellular protein homeostasis
EP4001402A1 (en) 2015-09-08 2022-05-25 FUJIFILM Cellular Dynamics, Inc. Macs-based purification of stem cell-derived retinal pigment epithelium
AU2016321170B2 (en) 2015-09-08 2022-09-01 Fujifilm Cellular Dynamics Method for reproducible differentiation of clinical-grade retinal pigment epithelium cells
ES2891087T3 (es) 2015-10-08 2022-01-26 Dna Twopointo Inc Vectores de ADN, trasposones y transposasas para la modificación del genoma eucariota
US11441126B2 (en) 2015-10-16 2022-09-13 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
ES2862676T3 (es) 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
WO2017183736A1 (ja) 2016-04-22 2017-10-26 国立大学法人京都大学 ドーパミン産生神経前駆細胞の製造方法
CN115960966A (zh) * 2016-04-29 2023-04-14 复诺健生物科技加拿大有限公司 在癌细胞中具有增强复制的hsv载体
GB201608944D0 (en) 2016-05-20 2016-07-06 Ospedale San Raffaele And Fond Telethon Gene Tharapy
US10221395B2 (en) 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
AU2017290805B2 (en) 2016-07-01 2023-11-16 Research Development Foundation Elimination of proliferating cells from stem cell-derived grafts
US11866733B2 (en) 2016-08-01 2024-01-09 University of Pittsburgh—of the Commonwealth System of Higher Education Human induced pluripotent stem cells for high efficiency genetic engineering
ES2985812T3 (es) 2016-09-09 2024-11-07 The Board Of Trustees Of The Leland Stanfordjunior Univ Edición genómica de precisión de alto rendimiento
JP7248571B2 (ja) 2016-10-05 2023-03-29 フジフィルム セルラー ダイナミクス，インコーポレイテッド ＭｅＣＰ２が破壊された誘導多能性幹細胞からの成熟系列の生成
JP7212615B2 (ja) * 2016-10-05 2023-01-25 フジフィルム セルラー ダイナミクス，インコーポレイテッド 多能性幹細胞からｈｌａホモ接合免疫細胞への指向分化方法
EP3538168B1 (en) 2016-11-09 2025-01-01 The United States of America, as represented by The Secretary, Department of Health and Human Services 3d vascularized human ocular tissue for cell therapy and drug discovery
CN110546267B (zh) * 2017-02-01 2024-09-13 哈佛学院董事及会员团体 提高细胞基因编辑效率的方法
CN106939299B (zh) * 2017-02-04 2022-11-15 滨州医学院 microRNA重编程体细胞为神经干细胞的制备方法及应用
AU2018220843B2 (en) 2017-02-14 2023-09-21 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
AU2018254442B2 (en) 2017-04-18 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
EP3621630A4 (en) 2017-06-14 2021-03-10 The Children's Medical Center PROGENITOR CELLS AND HEMATOPOIETIC STRAINS DERIVED FROM ENDOTHELIAL HEMOGENIC CELLS BY EPISOMIC PLASMID GENE TRANSFER
CA3097428A1 (en) 2018-04-20 2019-10-24 FUJIFILM Cellular Dynamics, Inc. Method for differentiation of ocular cells and use thereof
CN113226387B (zh) 2018-11-19 2024-02-23 美国政府(由卫生和人类服务部的部长所代表) 可生物降解的组织置换植入物及其用途
MX2021006208A (es) 2018-11-28 2021-10-01 Univ Texas Edición por multiplexación del genoma de células inmunitarias para mejorar la funcionalidad y resistencia al entorno supresor.
WO2020112493A1 (en) 2018-11-29 2020-06-04 Board Of Regents, The University Of Texas System Methods for ex vivo expansion of natural killer cells and use thereof
CN109679918A (zh) * 2018-12-25 2019-04-26 合肥中科干细胞再生医学有限公司 一种便捷的人诱导性多能干细胞的制备方法
CN109666650A (zh) * 2019-01-04 2019-04-23 北京航空航天大学 一种细胞重编程方法
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法
JP2022535192A (ja) 2019-05-14 2022-08-05 アレフ ファームス リミテッド 多能性細胞凝集体およびそれらの使用
EP4093857A4 (en) 2020-01-23 2024-02-21 The Children's Medical Center Corporation DIFFERENTIATION OF STROMA-FREE T CELLS FROM HUMAN PLURIPOTENT STEM CELLS
WO2021149824A1 (ja) * 2020-01-24 2021-07-29 アイ ピース， インコーポレイテッド 人工多能性幹細胞の作製方法
BR112022024031A2 (pt) 2020-05-29 2023-01-31 Fujifilm Cellular Dynamics Inc Agregados de células duplas de epitélio pigmentado da retina e fotorreceptor, e métodos de uso dos mesmos
US20230212509A1 (en) 2020-05-29 2023-07-06 FUJIFILM Cellular Dynamics, Inc. Bilayer of retinal pigmented epithelium and photoreceptors and use thereof
EP4182443A4 (en) * 2020-07-20 2025-02-12 CellResearch Corporation Pte Ltd METHOD FOR PRODUCING AN INDUCED PLURIPOTENT STEM CELL, INDUCED PLURIPOTENT STEM CELL AND METHOD FOR USING THE INDUCED PLURIPOTENT STEM CELL
CN114149974A (zh) * 2020-09-08 2022-03-08 是光隽恒(北京)生物科技有限公司 诱导间充质干细胞成为多能干细胞的方法
AU2021377699A1 (en) 2020-11-13 2023-06-15 Catamaran Bio, Inc. Genetically modified natural killer cells and methods of use thereof
US20220162288A1 (en) 2020-11-25 2022-05-26 Catamaran Bio, Inc. Cellular therapeutics engineered with signal modulators and methods of use thereof
GB202101107D0 (en) * 2021-01-27 2021-03-10 Oxford Genetics Ltd Method of genome editing
KR20240011831A (ko) 2021-05-26 2024-01-26 후지필름 셀룰러 다이내믹스, 인코포레이티드 만능 줄기 세포에서 유전자의 신속한 사일런싱을 방지하기 위한 방법
JP2024520423A (ja) 2021-05-28 2024-05-24 ザ ユナイテッド ステイツ オブ アメリカ， アズ リプレゼンテッド バイ ザ セクレタリー， デパートメント オブ ヘルス アンド ヒューマン サービシーズ 弱接着細胞を収容するための二次マトリックスを有する生分解性組織スキャフォールド
US20240271089A1 (en) 2021-05-28 2024-08-15 The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Methods to generate macular, central and peripheral retinal pigment epithelial cells
KR20240055811A (ko) 2021-09-10 2024-04-29 애질런트 테크놀로지스, 인크. 프라임 편집을 위한 화학적 변형을 갖는 가이드 rna
KR20240056604A (ko) 2021-09-13 2024-04-30 후지필름 셀룰러 다이내믹스, 인코포레이티드 수임 심장 전구세포의 제조 방법
CN113862221B (zh) * 2021-10-27 2024-04-30 上海爱萨尔生物科技有限公司 外周血单个核细胞的培养液及培养方法
CN119452078A (zh) 2022-06-29 2025-02-14 富士胶片控股美国公司 Ipsc来源的星形胶质细胞及其使用方法
CN115044590B (zh) * 2022-06-30 2023-08-15 昆明理工大学 p53基因突变体及其表达的蛋白在制备诊断和治疗肥厚型心肌病的药物中的应用
EP4594471A1 (en) 2022-09-30 2025-08-06 Fujifilm Cellular Dynamics, Inc. Methods for the production of cardiac fibroblasts
WO2024192329A1 (en) 2023-03-16 2024-09-19 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Methods for producing stable human chondroctyes and their use for promoting cartillage growth and repair
US20250362218A1 (en) 2024-05-21 2025-11-27 FUJIFILM Cellular Dynamics, Inc. Particle counting and biomass measurements of aggregated cell compositions

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
CA2360671A1 (en) 1999-01-29 2000-08-03 The Board Of Trustees Of The University Of Illinois P53 inhibitors and therapeutic use of the same
WO2000070070A1 (en) 1999-05-18 2000-11-23 Dnavec Research Inc. Paramyxoviridae virus vector defective in envelope gene
JP2008528038A (ja) 2005-01-31 2008-07-31 エス セル インターナショナル ピーティーイー リミテッド 胚性幹細胞の指示された分化及びその利用
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP4223769A3 (en) * 2005-12-13 2023-11-01 Kyoto University Nuclear reprogramming factor
WO2007080591A2 (en) 2006-01-11 2007-07-19 Technion Research & Development Foundation Ltd. Adult stem cell-derived connective tissue progenitors for tissue engineering
CN101743306A (zh) 2007-03-23 2010-06-16 威斯康星校友研究基金会 体细胞重编程
US7972849B2 (en) 2007-05-17 2011-07-05 Oregon Health & Science University Primate pluripotent stem cells produced by somatic cell nuclear transfer
AU2008297024B2 (en) * 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
AU2008286249B2 (en) 2007-12-10 2013-10-10 Kyoto University Efficient method for nuclear reprogramming
WO2009115295A1 (en) * 2008-03-17 2009-09-24 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
CN101550406B (zh) 2008-04-03 2016-02-10 北京大学 制备多潜能干细胞的方法,试剂盒及用途
KR101871192B1 (ko) 2008-06-04 2018-06-27 후지필름 셀룰러 다이내믹스, 인코포레이티드 비-바이러스 접근법을 사용한 ｉＰＳ 세포의 생산 방법
EP3231869A1 (en) * 2008-06-13 2017-10-18 Whitehead Institute for Biomedical Research Programming and reprogramming of cells
JP5340265B2 (ja) 2008-06-27 2013-11-13 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
WO2010013845A1 (en) 2008-07-30 2010-02-04 Kyoto University Method of efficiently establishing induced pluripotent stem cells
AU2008360135A1 (en) 2008-07-31 2010-02-04 Gifu University Efficient method for establishing induced pluripotent stem cells
JP2010273680A (ja) 2009-05-29 2010-12-09 Kyoto Univ 初期化因子が除去された人工多能性幹細胞の作製方法
SG10201608797WA (en) 2009-08-07 2016-12-29 Univ Kyoto Method of efficiently establishing induced pluripotent stem cells
CN102803476A (zh) 2009-09-14 2012-11-28 程临钊 血细胞重编程产生多潜能干细胞和多功能干细胞
KR101857302B1 (ko) 2010-02-16 2018-05-11 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도된 다능성 줄기 세포의 효율적 확립 방법
WO2011119942A1 (en) 2010-03-25 2011-09-29 Vistagen Therapeutics, Inc. Induction of ips cells using transient episomal vectors
EP2601289B1 (en) 2010-08-04 2017-07-12 Cellular Dynamics International, Inc. Reprogramming immortalized b cells
WO2013022022A1 (ja) 2011-08-08 2013-02-14 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
CA2874259C (en) 2012-05-23 2021-02-09 Kyoto University Highly efficient method for establishing induced pluripotent stem cell

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12195764B2 (en) 2020-12-29 2025-01-14 I Peace, Inc. Method for culturing cells into which reprogramming factor is introduced

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