JP4801258B2 - アフィニティ分離の方法及びそれに用いるリガンド - Google Patents

アフィニティ分離の方法及びそれに用いるリガンド Download PDF

Info

Publication number
JP4801258B2
JP4801258B2 JP2000577291A JP2000577291A JP4801258B2 JP 4801258 B2 JP4801258 B2 JP 4801258B2 JP 2000577291 A JP2000577291 A JP 2000577291A JP 2000577291 A JP2000577291 A JP 2000577291A JP 4801258 B2 JP4801258 B2 JP 4801258B2
Authority
JP
Japan
Prior art keywords
protein
asn
affinity
binding
residues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2000577291A
Other languages
English (en)
Japanese (ja)
Other versions
JP2002527107A (ja
JP2002527107A5 (enExample
Inventor
ユーレン、マーチアス
ホバー、ソフィア
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affibody AB
Original Assignee
Affibody AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affibody AB filed Critical Affibody AB
Publication of JP2002527107A publication Critical patent/JP2002527107A/ja
Publication of JP2002527107A5 publication Critical patent/JP2002527107A5/ja
Application granted granted Critical
Publication of JP4801258B2 publication Critical patent/JP4801258B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2000577291A 1998-10-21 1999-10-21 アフィニティ分離の方法及びそれに用いるリガンド Expired - Lifetime JP4801258B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9823071.7 1998-10-21
GBGB9823071.7A GB9823071D0 (en) 1998-10-21 1998-10-21 A method
PCT/GB1999/003484 WO2000023580A1 (en) 1998-10-21 1999-10-21 A method of affinity separation and ligands for use therein

Publications (3)

Publication Number Publication Date
JP2002527107A JP2002527107A (ja) 2002-08-27
JP2002527107A5 JP2002527107A5 (enExample) 2006-12-07
JP4801258B2 true JP4801258B2 (ja) 2011-10-26

Family

ID=10841043

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000577291A Expired - Lifetime JP4801258B2 (ja) 1998-10-21 1999-10-21 アフィニティ分離の方法及びそれに用いるリガンド

Country Status (12)

Country Link
US (2) US6831161B1 (enExample)
EP (2) EP1672068A1 (enExample)
JP (1) JP4801258B2 (enExample)
AT (1) ATE305970T1 (enExample)
AU (1) AU773368B2 (enExample)
CA (2) CA2347960C (enExample)
DE (1) DE69927598T2 (enExample)
DK (1) DK1123389T3 (enExample)
GB (1) GB9823071D0 (enExample)
NO (1) NO323994B1 (enExample)
WO (1) WO2000023580A1 (enExample)
ZA (1) ZA200103252B (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0200943D0 (sv) 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US7709209B2 (en) 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
GB0214007D0 (en) * 2002-06-18 2002-07-31 Common Services Agency Removal of prion infectivity
EP1634076A2 (en) * 2003-06-10 2006-03-15 CatchMabs B.V. Binding peptides: methods for their generation and use
SE0301936D0 (sv) * 2003-06-30 2003-06-30 Affibody Ab New polypeptide
WO2005003156A1 (en) * 2003-07-04 2005-01-13 Affibody Ab Polypeptides having binding affinity for her2
WO2005097202A2 (en) 2004-04-06 2005-10-20 Affibody Ab Use of serum albumin binding peptides conjugates for the preparation of a medicament
AU2007300751B2 (en) 2006-09-29 2012-01-19 Cytiva Bioprocess R&D Ab Chromatography ligand comprising Domain C from Staphyloccocus aureus protein A for antibody isolation
EP2190863B1 (en) * 2007-07-31 2015-09-02 Affibody AB New albumin binding compositions, methods and uses
GB2452301A (en) * 2007-08-30 2009-03-04 Ge Healthcare Bio Sciences Ab Sterilization Method
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
WO2010035757A1 (ja) * 2008-09-25 2010-04-01 Jsr株式会社 アフィニティークロマトグラフィー用充填剤
JP5229888B2 (ja) * 2008-09-30 2013-07-03 独立行政法人産業技術総合研究所 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
EP2412809B1 (en) 2009-03-24 2017-08-09 Kaneka Corporation Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand
WO2011118699A1 (ja) 2010-03-24 2011-09-29 株式会社カネカ 免疫グロブリンに特異的に結合するタンパク質および免疫グロブリン結合性アフィニティーリガンド
JP5997176B2 (ja) * 2010-12-21 2016-09-28 ザ ユニバーシティ オブ ウエスタン オンタリオＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｗｅｓｔｅｒｎ Ｏｎｔａｒｉｏ プロテインａの新規アルカリ抵抗性変異体及びアフィニティークロマトグラフィーにおけるその使用
CN103270044B (zh) 2010-12-21 2016-03-09 Jsr株式会社 亲和色谱用载体和分离免疫球蛋白的方法
JP6038774B2 (ja) 2011-03-24 2016-12-07 株式会社カネカ タンパク性物質結合性低分子化合物
SG193950A1 (en) 2011-03-25 2013-11-29 Kaneka Corp Novel immunoglobulin-binding polypeptide
SG186552A1 (en) 2011-06-08 2013-01-30 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
WO2014076179A1 (en) 2012-11-14 2014-05-22 Affibody Ab New polypeptide
WO2014076177A1 (en) * 2012-11-14 2014-05-22 Affibody Ab New polypeptide
US9255262B2 (en) * 2013-03-06 2016-02-09 Vision Global Holdings Ltd. Albumin-binding arginine deminase and the use thereof
USRE48805E1 (en) 2013-03-06 2021-11-02 Vision Global Holdings Ltd. Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein
US11014129B2 (en) 2013-09-04 2021-05-25 Emd Millipore Corporation Methods of cleaning a protein A based affinity chromatography column
JP6491600B2 (ja) 2013-09-27 2019-03-27 株式会社カネカ アルカリ水溶液を用いた多孔質セルロースビーズの製造方法
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
JP6369807B2 (ja) * 2014-10-21 2018-08-08 株式会社プロテイン・エクスプレス プロテインｌ変異体
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
EP3276002B1 (en) 2015-03-26 2020-04-29 JSR Corporation Immunoglobulin-binding protein and affinity carrier using same
EP3875477A1 (en) 2015-04-17 2021-09-08 Alpine Immune Sciences, Inc. Immunomodulatory proteins with tunable affinities
EP3375873B1 (en) 2015-10-22 2025-07-02 Protenova Co., Ltd. Immunoglobulin-binding polypeptide
WO2017191748A1 (ja) * 2016-05-02 2017-11-09 株式会社カネカ 改変型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
EP3592759A1 (en) 2017-03-07 2020-01-15 JMVA Biotech AB Modified adsorptive surfaces
US11753449B2 (en) 2017-09-25 2023-09-12 Jsr Corporation Immunoglobulin binding protein, and affinity support using same
JP7724061B2 (ja) 2018-04-11 2025-08-15 インヒブルクス バイオサイエンシズ インコーポレイテッド 制約されたｃｄ３結合を有する多重特異性ポリペプチド構築物ならびに関連する方法および使用
US12428451B2 (en) 2018-08-24 2025-09-30 Jsr Corporation Immunoglobulin-binding protein, and affinity carrier using same
JP2023551353A (ja) 2020-10-13 2023-12-08 アヴィタイド エルエルシー ３へリックスバンドルタンパク質の親和性リガンドライブラリー及びその使用
CN116670150A (zh) 2020-12-22 2023-08-29 普罗泰纳瓦株式会社 免疫球蛋白结合多肽
WO2022178396A1 (en) 2021-02-19 2022-08-25 Avitide LLC Aav2 affinity agents
GB2609185A (en) 2021-05-24 2023-02-01 Astrea Uk Services Ltd Scaffold for isolation of a biomolecule
EP4541814A1 (en) * 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Stabilized polypeptides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462046C (sv) * 1982-08-23 1998-04-27 Pharmacia Biotech Ab Hybrid-DNA-vektor innehållande DNA-sekvens från Staphylococcus aureus, förfarande för dess framställning och bakterie innehållande densamma
US5401832A (en) * 1984-12-24 1995-03-28 Merck & Co., Inc. Brain derived and recombinant acidic fibroblast growth factor
US5089473A (en) * 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
EP0863210A3 (en) * 1991-07-25 1999-09-22 Oriental Yeast Co., Ltd. Immunoglobulin-binding artificial protein
JP2584697B2 (ja) * 1991-08-23 1997-02-26 オリエンタル酵母工業株式会社 ＩｇＧ精製用組換え型プロテインＡ
DE59306762D1 (de) * 1992-04-16 1997-07-24 Merck Patent Gmbh Aktivierte Trägermaterialien, ihre Herstellung und Verwendung
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
EP0968290B1 (en) * 1997-03-12 2004-12-15 SmithKline Beecham Corporation Anti-alphavbeta3 humanized monoclonal antibodies

Also Published As

Publication number Publication date
ZA200103252B (en) 2002-07-22
NO20011969D0 (no) 2001-04-20
CA2633345A1 (en) 2000-04-27
JP2002527107A (ja) 2002-08-27
DK1123389T3 (da) 2006-01-16
CA2347960A1 (en) 2000-04-27
CA2347960C (en) 2008-08-05
CA2633345C (en) 2013-02-26
AU6353299A (en) 2000-05-08
EP1123389A1 (en) 2001-08-16
GB9823071D0 (en) 1998-12-16
AU773368B2 (en) 2004-05-20
ATE305970T1 (de) 2005-10-15
DE69927598D1 (de) 2006-02-16
DE69927598T2 (de) 2006-07-13
NO323994B1 (no) 2007-07-30
US20050100970A1 (en) 2005-05-12
NO20011969L (no) 2001-06-19
EP1672068A1 (en) 2006-06-21
WO2000023580A1 (en) 2000-04-27
US6831161B1 (en) 2004-12-14
EP1123389B1 (en) 2005-10-05

Similar Documents

Publication Publication Date Title
JP4801258B2 (ja) アフィニティ分離の方法及びそれに用いるリガンド
KR101067181B1 (ko) 돌연변이된 면역글로불린-결합 단백질
EP2690173B1 (en) Protein for affinity-separation matrix
JP4089920B2 (ja) 細菌レセプター構造体
Bratkovič Progress in phage display: evolution of the technique and its applications
EP1992692B1 (en) Immunoglobulin affinity ligand
JP4578690B2 (ja) ヒト第ｖｉｉｉ因子の少なくとも１ドメインと相互作用するスタフィロコッカスタンパク質ａ（ｓｐａ）由来のｂまたはｚドメインの誘導体
KR20190044059A (ko) 신규한 알칼리 안정성 면역글로불린 결합 단백질
JP7744026B2 (ja) スプリットインテインシステムを使用したタンパク質精製
Minakuchi et al. Remarkable alkaline stability of an engineered protein A as immunoglobulin affinity ligand: C domain having only one amino acid substitution
US20180215785A1 (en) Method for purifying antibody-like protein
EP1252319A2 (en) Combinatorial protein domains
KR20110003547A (ko) 인위적인 단백질 스캐폴드
EP3327029A1 (en) ANTIBODY-BINDING PROTEIN HAVING REDUCED ANTIBODY-BINDING CAPACITY IN ACIDIC pH REGIONS
WO2018019537A1 (en) Method for obtaining aptamers
WO2002012277A2 (en) Hybrid combinatorial proteins made from reshuffling of differently folded domains

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20061019

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20061019

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20061020

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20061020

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20061018

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20070326

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20070326

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090908

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20091204

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100629

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20100902

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110712

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110805

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140812

Year of fee payment: 3

R150 Certificate of patent or registration of utility model

Ref document number: 4801258

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term