DE69927598D1 - Verfahren zur affinitätstrennung und ligande zur pertinenten verwendung - Google Patents

Verfahren zur affinitätstrennung und ligande zur pertinenten verwendung

Info

Publication number
DE69927598D1
DE69927598D1 DE69927598T DE69927598T DE69927598D1 DE 69927598 D1 DE69927598 D1 DE 69927598D1 DE 69927598 T DE69927598 T DE 69927598T DE 69927598 T DE69927598 T DE 69927598T DE 69927598 D1 DE69927598 D1 DE 69927598D1
Authority
DE
Germany
Prior art keywords
protein
affinity separation
ligands
asn
residues
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69927598T
Other languages
English (en)
Other versions
DE69927598T2 (de
Inventor
Mathias Uhlen
Sophia Hober
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affibody AB
Original Assignee
Affibody AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affibody AB filed Critical Affibody AB
Publication of DE69927598D1 publication Critical patent/DE69927598D1/de
Application granted granted Critical
Publication of DE69927598T2 publication Critical patent/DE69927598T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69927598T 1998-10-21 1999-10-21 Verfahren zur Affinitätstrennung und Liganden zur pertinenten Verwendung Expired - Lifetime DE69927598T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9823071.7A GB9823071D0 (en) 1998-10-21 1998-10-21 A method
GB9823071 1998-10-21
PCT/GB1999/003484 WO2000023580A1 (en) 1998-10-21 1999-10-21 A method of affinity separation and ligands for use therein

Publications (2)

Publication Number Publication Date
DE69927598D1 true DE69927598D1 (de) 2006-02-16
DE69927598T2 DE69927598T2 (de) 2006-07-13

Family

ID=10841043

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69927598T Expired - Lifetime DE69927598T2 (de) 1998-10-21 1999-10-21 Verfahren zur Affinitätstrennung und Liganden zur pertinenten Verwendung

Country Status (12)

Country Link
US (2) US6831161B1 (de)
EP (2) EP1672068A1 (de)
JP (1) JP4801258B2 (de)
AT (1) ATE305970T1 (de)
AU (1) AU773368B2 (de)
CA (2) CA2347960C (de)
DE (1) DE69927598T2 (de)
DK (1) DK1123389T3 (de)
GB (1) GB9823071D0 (de)
NO (1) NO323994B1 (de)
WO (1) WO2000023580A1 (de)
ZA (1) ZA200103252B (de)

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SE0200943D0 (sv) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
US7709209B2 (en) 2002-03-25 2010-05-04 Ge Healthcare Bio-Sciences Ab Protein ligands
GB0214007D0 (en) * 2002-06-18 2002-07-31 Common Services Agency Removal of prion infectivity
AU2004245429A1 (en) * 2003-06-10 2004-12-16 Farallone Holding Bv Binding peptides: methods for their generation and use
SE0301936D0 (sv) * 2003-06-30 2003-06-30 Affibody Ab New polypeptide
WO2005003156A1 (en) * 2003-07-04 2005-01-13 Affibody Ab Polypeptides having binding affinity for her2
US8642743B2 (en) 2004-04-06 2014-02-04 Affibody Ab Method for reducing the immune response to a biologically active protein
CN101522278B (zh) 2006-09-29 2016-08-10 通用电气健康护理生物科学股份公司 用于抗体分离的包含来自金黄色葡萄球菌a蛋白的结构域c的层析配体
EP2190863B1 (de) * 2007-07-31 2015-09-02 Affibody AB Neue albuminbindende zusammensetzungen, verfahren und anwendungen
GB2452301A (en) 2007-08-30 2009-03-04 Ge Healthcare Bio Sciences Ab Sterilization Method
US8592555B2 (en) * 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
US8846877B2 (en) * 2008-09-25 2014-09-30 Jsr Corporation Packing material for affinity chromatography
JP5229888B2 (ja) * 2008-09-30 2013-07-03 独立行政法人産業技術総合研究所 弱酸性域での易解離性を向上したプロテインａ変異型タンパク質及び抗体捕捉剤
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
EP2412809B1 (de) 2009-03-24 2017-08-09 Kaneka Corporation Protein mit immunglobulinaffinität und ligand mit affinität für immunglobulinbindung
JP5952185B2 (ja) 2010-03-24 2016-07-13 株式会社カネカ 免疫グロブリンに特異的に結合するタンパク質および免疫グロブリン結合性アフィニティーリガンド
CN103270044B (zh) 2010-12-21 2016-03-09 Jsr株式会社 亲和色谱用载体和分离免疫球蛋白的方法
JP5997176B2 (ja) * 2010-12-21 2016-09-28 ザ ユニバーシティ オブ ウエスタン オンタリオＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｗｅｓｔｅｒｎ Ｏｎｔａｒｉｏ プロテインａの新規アルカリ抵抗性変異体及びアフィニティークロマトグラフィーにおけるその使用
JP6038774B2 (ja) 2011-03-24 2016-12-07 株式会社カネカ タンパク性物質結合性低分子化合物
WO2012133342A1 (ja) 2011-03-25 2012-10-04 株式会社カネカ 免疫グロブリン結合性新規ポリペプチド
SG186552A1 (en) 2011-06-08 2013-01-30 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
WO2014076177A1 (en) * 2012-11-14 2014-05-22 Affibody Ab New polypeptide
WO2014076179A1 (en) 2012-11-14 2014-05-22 Affibody Ab New polypeptide
US9255262B2 (en) * 2013-03-06 2016-02-09 Vision Global Holdings Ltd. Albumin-binding arginine deminase and the use thereof
USRE48805E1 (en) 2013-03-06 2021-11-02 Vision Global Holdings Ltd. Method for cancer targeting treatment and detection of arginine using albumin-binding arginine deiminase fusion protein
CA2923133C (en) 2013-09-04 2019-05-28 Emd Millipore Corporation Methods of cleaning a protein a based affinity chromatography column
EP3459631A1 (de) 2013-09-27 2019-03-27 Kaneka Corporation Verfahren zur herstellung poröser celluloseperlen unter verwendung einer wässrigen alkalilösung und durch dieses verfahren hergestellte poröse celluloseperlen
SG10201804200WA (en) 2013-12-12 2018-07-30 Emd Millipore Corp Protein separations using an acrylamide containing filter
JP6369807B2 (ja) * 2014-10-21 2018-08-08 株式会社プロテイン・エクスプレス プロテインｌ変異体
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
EP3252158A4 (de) 2015-01-26 2018-07-18 Kaneka Corporation Mutantes immunglobulin-kappa-ketten-bindendes protein mit variabler region
JP6705806B2 (ja) 2015-03-26 2020-06-03 Jsr株式会社 イムノグロブリン結合タンパク質およびそれを用いたアフィニティー担体
CN107969128A (zh) 2015-04-17 2018-04-27 高山免疫科学股份有限公司 具有可调的亲和力的免疫调节蛋白
CN108291220B (zh) 2015-10-22 2022-10-28 普罗泰纳瓦株式会社 免疫球蛋白结合多肽
WO2017191748A1 (ja) * 2016-05-02 2017-11-09 株式会社カネカ 改変型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
WO2018162501A1 (en) 2017-03-07 2018-09-13 Jmva Biotech Ab Modified adsorptive surfaces
EP3689894A4 (de) 2017-09-25 2021-12-22 JSR Corporation Immunglobulin-bindendes protein und affinitätsträger damit
EP3842536A4 (de) 2018-08-24 2023-01-18 JSR Corporation Immunglobulin-bindendes protein und affinitätsträger damit
KR20230112616A (ko) 2020-10-13 2023-07-27 아비타이드 엘엘씨 3중-나선 번들 단백질들의 친화성 리간드 라이브러리 및 이의 용도
EP4269590A1 (de) 2020-12-22 2023-11-01 Protenova Co., Ltd. Immunglobulinbindendes polypeptid
CN117241866A (zh) 2021-02-19 2023-12-15 艾维泰有限责任公司 Aav2亲和剂
GB2609185A (en) 2021-05-24 2023-02-01 Astrea Uk Services Ltd Scaffold for isolation of a biomolecule

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SE8204810L (sv) * 1982-08-23 1984-02-24 Pharmacia Fine Chemicals Ab Hybrid-dna-molekyl, transformerad mikroorganism och sett att framstella protein a
US5401832A (en) * 1984-12-24 1995-03-28 Merck & Co., Inc. Brain derived and recombinant acidic fibroblast growth factor
US5089473A (en) * 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
EP0863210A3 (de) * 1991-07-25 1999-09-22 Oriental Yeast Co., Ltd. Künstliches Immunglobulinkombinierendes Protein
JP2584697B2 (ja) * 1991-08-23 1997-02-26 オリエンタル酵母工業株式会社 ＩｇＧ精製用組換え型プロテインＡ
EP0565978B1 (de) * 1992-04-16 1997-06-18 MERCK PATENT GmbH Aktivierte Trägermaterialien, ihre Herstellung und Verwendung
SE9400088D0 (sv) * 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
WO1998040488A1 (en) * 1997-03-12 1998-09-17 Smithkline Beecham Corporation Anti-alphabeta3 humanized monoclonal antibodies

Also Published As

Publication number Publication date
EP1123389B1 (de) 2005-10-05
JP2002527107A (ja) 2002-08-27
US6831161B1 (en) 2004-12-14
NO20011969D0 (no) 2001-04-20
ATE305970T1 (de) 2005-10-15
CA2347960A1 (en) 2000-04-27
JP4801258B2 (ja) 2011-10-26
NO323994B1 (no) 2007-07-30
CA2633345C (en) 2013-02-26
GB9823071D0 (en) 1998-12-16
NO20011969L (no) 2001-06-19
CA2347960C (en) 2008-08-05
DE69927598T2 (de) 2006-07-13
AU773368B2 (en) 2004-05-20
EP1672068A1 (de) 2006-06-21
AU6353299A (en) 2000-05-08
CA2633345A1 (en) 2000-04-27
WO2000023580A1 (en) 2000-04-27
US20050100970A1 (en) 2005-05-12
DK1123389T3 (da) 2006-01-16
ZA200103252B (en) 2002-07-22
EP1123389A1 (de) 2001-08-16

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: AFFIBODY AB, BROMMA, SE