JP3350442B2 - 顕微鏡システム - Google Patents
顕微鏡システムInfo
- Publication number
- JP3350442B2 JP3350442B2 JP09792498A JP9792498A JP3350442B2 JP 3350442 B2 JP3350442 B2 JP 3350442B2 JP 09792498 A JP09792498 A JP 09792498A JP 9792498 A JP9792498 A JP 9792498A JP 3350442 B2 JP3350442 B2 JP 3350442B2
- Authority
- JP
- Japan
- Prior art keywords
- wavelength
- light
- laser
- microscope system
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000003287 optical effect Effects 0.000 claims description 227
- 230000005281 excited state Effects 0.000 claims description 125
- 238000009826 distribution Methods 0.000 claims description 66
- 230000005283 ground state Effects 0.000 claims description 48
- 238000000034 method Methods 0.000 claims description 47
- 230000008569 process Effects 0.000 claims description 41
- -1 4-Biphenylyl Chemical group 0.000 claims description 40
- 239000007789 gas Substances 0.000 claims description 34
- 239000013078 crystal Substances 0.000 claims description 33
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 claims description 32
- VLTRZXGMWDSKGL-UHFFFAOYSA-M perchlorate Inorganic materials [O-]Cl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-M 0.000 claims description 32
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 28
- 239000004065 semiconductor Substances 0.000 claims description 28
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims description 25
- 230000005284 excitation Effects 0.000 claims description 24
- 230000010355 oscillation Effects 0.000 claims description 24
- 230000005684 electric field Effects 0.000 claims description 21
- 229960000956 coumarin Drugs 0.000 claims description 20
- 235000001671 coumarin Nutrition 0.000 claims description 16
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims description 15
- 238000001069 Raman spectroscopy Methods 0.000 claims description 15
- CVAVMIODJQHEEH-UHFFFAOYSA-O rhodamine B(1+) Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O CVAVMIODJQHEEH-UHFFFAOYSA-O 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 15
- 230000001678 irradiating effect Effects 0.000 claims description 13
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical group C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 11
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 230000001143 conditioned effect Effects 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 7
- 125000000031 ethylamino group Chemical group [H]C([H])([H])C([H])([H])N([H])[*] 0.000 claims description 7
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 claims description 6
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 6
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 6
- 235000010290 biphenyl Nutrition 0.000 claims description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 6
- 239000010453 quartz Substances 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 150000001555 benzenes Chemical class 0.000 claims description 5
- GZTMNDOZYLMFQE-UHFFFAOYSA-N coumarin 500 Chemical compound FC(F)(F)C1=CC(=O)OC2=CC(NCC)=CC=C21 GZTMNDOZYLMFQE-UHFFFAOYSA-N 0.000 claims description 5
- 229940075420 xanthine Drugs 0.000 claims description 5
- NUMOZCKMSNMNGZ-UHFFFAOYSA-M xanthylium;perchlorate Chemical compound [O-]Cl(=O)(=O)=O.C1=CC=CC2=CC3=CC=CC=C3[O+]=C21 NUMOZCKMSNMNGZ-UHFFFAOYSA-M 0.000 claims description 5
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims description 4
- VYXSBFYARXAAKO-UHFFFAOYSA-N ethyl 2-[3-(ethylamino)-6-ethylimino-2,7-dimethylxanthen-9-yl]benzoate;hydron;chloride Chemical compound [Cl-].C1=2C=C(C)C(NCC)=CC=2OC2=CC(=[NH+]CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-UHFFFAOYSA-N 0.000 claims description 4
- 150000003212 purines Chemical class 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- JKXWXYURKUEZHV-UHFFFAOYSA-M (2z)-1,3,3-trimethyl-2-[(2e)-7-(1,3,3-trimethylindol-1-ium-2-yl)hepta-2,4,6-trienylidene]indole;iodide Chemical compound [I-].CC1(C)C2=CC=CC=C2N(C)C1=CC=CC=CC=CC1=[N+](C)C2=CC=CC=C2C1(C)C JKXWXYURKUEZHV-UHFFFAOYSA-M 0.000 claims description 3
- ZFYDGPJXMYCXNV-UHFFFAOYSA-M 1-ethyl-2-[5-(1-ethylquinolin-1-ium-2-yl)penta-2,4-dienylidene]quinoline;iodide Chemical compound [I-].C1=CC2=CC=CC=C2N(CC)C1=CC=CC=CC1=CC=C(C=CC=C2)C2=[N+]1CC ZFYDGPJXMYCXNV-UHFFFAOYSA-M 0.000 claims description 3
- HXWQJYVUJPBQEW-VAWYXSNFSA-N 1-phenyl-4-[(e)-2-(4-phenylphenyl)ethenyl]benzene Chemical compound C=1C=C(C=2C=CC=CC=2)C=CC=1/C=C/C(C=C1)=CC=C1C1=CC=CC=C1 HXWQJYVUJPBQEW-VAWYXSNFSA-N 0.000 claims description 3
- IVVYVRLKDGGUEL-UHFFFAOYSA-N 2,5-bis(4-phenylphenyl)-1,3,4-oxadiazole Chemical compound C1=CC=CC=C1C1=CC=C(C=2OC(=NN=2)C=2C=CC(=CC=2)C=2C=CC=CC=2)C=C1 IVVYVRLKDGGUEL-UHFFFAOYSA-N 0.000 claims description 3
- DDZJGFHXUOWOSL-UHFFFAOYSA-N 2,5-bis(4-phenylphenyl)-1,3-oxazole Chemical compound C=1N=C(C=2C=CC(=CC=2)C=2C=CC=CC=2)OC=1C(C=C1)=CC=C1C1=CC=CC=C1 DDZJGFHXUOWOSL-UHFFFAOYSA-N 0.000 claims description 3
- CNRNYORZJGVOSY-UHFFFAOYSA-N 2,5-diphenyl-1,3-oxazole Chemical compound C=1N=C(C=2C=CC=CC=2)OC=1C1=CC=CC=C1 CNRNYORZJGVOSY-UHFFFAOYSA-N 0.000 claims description 3
- VUPDHIIPAKIKAB-UHFFFAOYSA-N 2,5-diphenylfuran Chemical compound C=1C=C(C=2C=CC=CC=2)OC=1C1=CC=CC=C1 VUPDHIIPAKIKAB-UHFFFAOYSA-N 0.000 claims description 3
- ZTKQHJHANLVEBM-UHFFFAOYSA-N 2-[3-(ethylamino)-6-ethylimino-2,7-dimethylxanthen-9-yl]benzoic acid Chemical compound C1=2C=C(C)C(NCC)=CC=2OC2=CC(=NCC)C(C)=CC2=C1C1=CC=CC=C1C(O)=O ZTKQHJHANLVEBM-UHFFFAOYSA-N 0.000 claims description 3
- OYVFJKVYVDYPFV-UHFFFAOYSA-M 3-ethyl-2-[7-(3-ethyl-1,3-benzothiazol-3-ium-2-yl)hepta-2,4,6-trienylidene]-1,3-benzothiazole;iodide Chemical compound [I-].S1C2=CC=CC=C2[N+](CC)=C1/C=C/C=C/C=C/C=C1/N(CC)C2=CC=CC=C2S1 OYVFJKVYVDYPFV-UHFFFAOYSA-M 0.000 claims description 3
- LJMMZIHDOKOJCM-UHFFFAOYSA-N 4-[2-[4-(2-iodoethyl)-1,3-benzothiazol-2-yl]ethenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C=CC1=NC2=C(CCI)C=CC=C2S1 LJMMZIHDOKOJCM-UHFFFAOYSA-N 0.000 claims description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims description 3
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 claims description 3
- 239000005711 Benzoic acid Substances 0.000 claims description 3
- CLDZYSUDOQXJOU-UHFFFAOYSA-M C5-oxacyanine Chemical compound [I-].O1C2=CC=CC=C2[N+](CC)=C1C=CC=CC=C1N(CC)C2=CC=CC=C2O1 CLDZYSUDOQXJOU-UHFFFAOYSA-M 0.000 claims description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- UPZKDDJKJWYWHQ-UHFFFAOYSA-O [6-amino-9-(2-carboxyphenyl)xanthen-3-ylidene]azanium Chemical compound C=12C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C2C=1C1=CC=CC=C1C(O)=O UPZKDDJKJWYWHQ-UHFFFAOYSA-O 0.000 claims description 3
- 229910052786 argon Inorganic materials 0.000 claims description 3
- 125000005605 benzo group Chemical group 0.000 claims description 3
- 235000010233 benzoic acid Nutrition 0.000 claims description 3
- 239000000298 carbocyanine Substances 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- GLNDAGDHSLMOKX-UHFFFAOYSA-N coumarin 120 Chemical compound C1=C(N)C=CC2=C1OC(=O)C=C2C GLNDAGDHSLMOKX-UHFFFAOYSA-N 0.000 claims description 3
- AFYCEAFSNDLKSX-UHFFFAOYSA-N coumarin 460 Chemical compound CC1=CC(=O)OC2=CC(N(CC)CC)=CC=C21 AFYCEAFSNDLKSX-UHFFFAOYSA-N 0.000 claims description 3
- MZSOXGPKUOAXNY-UHFFFAOYSA-N coumarin 6h Chemical compound C1CCC2=C(OC(=O)C=C3)C3=CC3=C2N1CCC3 MZSOXGPKUOAXNY-UHFFFAOYSA-N 0.000 claims description 3
- CEJANLKHJMMNQB-UHFFFAOYSA-M cryptocyanin Chemical compound [I-].C12=CC=CC=C2N(CC)C=CC1=CC=CC1=CC=[N+](CC)C2=CC=CC=C12 CEJANLKHJMMNQB-UHFFFAOYSA-M 0.000 claims description 3
- 125000004494 ethyl ester group Chemical group 0.000 claims description 3
- QUSNBJAOOMFDIB-UHFFFAOYSA-O ethylaminium Chemical compound CC[NH3+] QUSNBJAOOMFDIB-UHFFFAOYSA-O 0.000 claims description 3
- QDLAGTHXVHQKRE-UHFFFAOYSA-N lichenxanthone Natural products COC1=CC(O)=C2C(=O)C3=C(C)C=C(OC)C=C3OC2=C1 QDLAGTHXVHQKRE-UHFFFAOYSA-N 0.000 claims description 3
- WCPAKWJPBJAGKN-UHFFFAOYSA-N oxadiazole Chemical compound C1=CON=N1 WCPAKWJPBJAGKN-UHFFFAOYSA-N 0.000 claims description 3
- CIYKWVNUXJDNNK-UHFFFAOYSA-N oxazine-750 Chemical compound N1=C2C3=CC=CC=C3C(NCC)=CC2=[O+]C2=C1C=C1CCCN3CCCC2=C13 CIYKWVNUXJDNNK-UHFFFAOYSA-N 0.000 claims description 3
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 3
- 229950004354 phosphorylcholine Drugs 0.000 claims description 3
- MUSLHCJRTRQOSP-UHFFFAOYSA-N rhodamine 101 Chemical compound [O-]C(=O)C1=CC=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MUSLHCJRTRQOSP-UHFFFAOYSA-N 0.000 claims description 3
- 239000010979 ruby Substances 0.000 claims description 3
- 229910001750 ruby Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 159000000000 sodium salts Chemical class 0.000 claims description 3
- COIVODZMVVUETJ-UHFFFAOYSA-N sulforhodamine 101 Chemical compound OS(=O)(=O)C1=CC(S([O-])(=O)=O)=CC=C1C1=C(C=C2C3=C4CCCN3CCC2)C4=[O+]C2=C1C=C1CCCN3CCCC2=C13 COIVODZMVVUETJ-UHFFFAOYSA-N 0.000 claims description 3
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims description 3
- OOBOPFKBJZPFHL-UHFFFAOYSA-M 1',2',4',5'-tetramethyl-6',7-dioxospiro[acridine-9,3'-cyclohexa-1,4-diene]-2-olate Chemical compound CC1=C(C)C(=O)C(C)=C(C)C21C1=CC([O-])=CC=C1N=C1C=CC(=O)C=C12 OOBOPFKBJZPFHL-UHFFFAOYSA-M 0.000 claims description 2
- CYGYCRFYLWJRAO-UHFFFAOYSA-N 1,3-dibutyl-5-[4-[4-[4-(3,5-dibutylphenyl)phenyl]phenyl]phenyl]-2-phenylbenzene Chemical group CCCCc1cc(CCCC)cc(c1)-c1ccc(cc1)-c1ccc(cc1)-c1ccc(cc1)-c1cc(CCCC)c(c(CCCC)c1)-c1ccccc1 CYGYCRFYLWJRAO-UHFFFAOYSA-N 0.000 claims description 2
- JCRAIAVOLCUDBD-UHFFFAOYSA-N 1,3-ditert-butyl-5-[4-[4-[4-(3,5-ditert-butylphenyl)phenyl]phenyl]phenyl]benzene Chemical group CC(C)(C)C1=CC(C(C)(C)C)=CC(C=2C=CC(=CC=2)C=2C=CC(=CC=2)C=2C=CC(=CC=2)C=2C=C(C=C(C=2)C(C)(C)C)C(C)(C)C)=C1 JCRAIAVOLCUDBD-UHFFFAOYSA-N 0.000 claims description 2
- VFNKZQNIXUFLBC-UHFFFAOYSA-N 2',7'-dichlorofluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(Cl)=C(O)C=C1OC1=C2C=C(Cl)C(O)=C1 VFNKZQNIXUFLBC-UHFFFAOYSA-N 0.000 claims description 2
- DXWHZJXKTHGHQF-UHFFFAOYSA-N 2-butyl-6-(butylamino)benzo[de]isoquinoline-1,3-dione Chemical compound O=C1N(CCCC)C(=O)C2=CC=CC3=C2C1=CC=C3NCCCC DXWHZJXKTHGHQF-UHFFFAOYSA-N 0.000 claims description 2
- WWVFJJKBBZXWFV-UHFFFAOYSA-N 2-naphthalen-1-yl-5-phenyl-1,3-oxazole Chemical compound C=1N=C(C=2C3=CC=CC=C3C=CC=2)OC=1C1=CC=CC=C1 WWVFJJKBBZXWFV-UHFFFAOYSA-N 0.000 claims description 2
- REHISTNPTFCXKS-UHFFFAOYSA-N 4-[2-[3-(iodomethyl)pyridin-2-yl]ethenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C=CC1=NC=CC=C1CI REHISTNPTFCXKS-UHFFFAOYSA-N 0.000 claims description 2
- QIJYPXSQECGLIG-UHFFFAOYSA-N 4-buta-1,3-dienyl-n,n-dimethylaniline Chemical compound CN(C)C1=CC=C(C=CC=C)C=C1 QIJYPXSQECGLIG-UHFFFAOYSA-N 0.000 claims description 2
- XDTYUYVIGLIFCW-UHFFFAOYSA-N 4-phenylbenzenesulfonic acid Chemical compound C1=CC(S(=O)(=O)O)=CC=C1C1=CC=CC=C1 XDTYUYVIGLIFCW-UHFFFAOYSA-N 0.000 claims description 2
- FIZZUEJIOKEFFZ-UHFFFAOYSA-M C3-oxacyanine Chemical compound [I-].O1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2O1 FIZZUEJIOKEFFZ-UHFFFAOYSA-M 0.000 claims description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 2
- ZCQWOFVYLHDMMC-UHFFFAOYSA-N Oxazole Chemical compound C1=COC=N1 ZCQWOFVYLHDMMC-UHFFFAOYSA-N 0.000 claims description 2
- RFRXIWQYSOIBDI-UHFFFAOYSA-N benzarone Chemical compound CCC=1OC2=CC=CC=C2C=1C(=O)C1=CC=C(O)C=C1 RFRXIWQYSOIBDI-UHFFFAOYSA-N 0.000 claims description 2
- XZCJVWCMJYNSQO-UHFFFAOYSA-N butyl pbd Chemical compound C1=CC(C(C)(C)C)=CC=C1C1=NN=C(C=2C=CC(=CC=2)C=2C=CC=CC=2)O1 XZCJVWCMJYNSQO-UHFFFAOYSA-N 0.000 claims description 2
- 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 claims description 2
- AMLQYWKJHVIKSB-UHFFFAOYSA-L dipotassium;4-[4-[4-(4-sulfonatophenyl)phenyl]phenyl]benzenesulfonate Chemical compound [K+].[K+].C1=CC(S(=O)(=O)[O-])=CC=C1C1=CC=C(C=2C=CC(=CC=2)C=2C=CC(=CC=2)S([O-])(=O)=O)C=C1 AMLQYWKJHVIKSB-UHFFFAOYSA-L 0.000 claims description 2
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- QKLPIYTUUFFRLV-UHFFFAOYSA-N 1,4-bis[2-(2-methylphenyl)ethenyl]benzene Chemical compound CC1=CC=CC=C1C=CC(C=C1)=CC=C1C=CC1=CC=CC=C1C QKLPIYTUUFFRLV-UHFFFAOYSA-N 0.000 claims 1
- 125000003349 3-pyridyl group Chemical group N1=C([H])C([*])=C([H])C([H])=C1[H] 0.000 claims 1
- NRZJOTSUPLCYDJ-UHFFFAOYSA-N 7-(ethylamino)-6-methyl-4-(trifluoromethyl)chromen-2-one Chemical compound O1C(=O)C=C(C(F)(F)F)C2=C1C=C(NCC)C(C)=C2 NRZJOTSUPLCYDJ-UHFFFAOYSA-N 0.000 claims 1
- 229910018072 Al 2 O 3 Inorganic materials 0.000 claims 1
- JGLWGLKNDHZFAP-UHFFFAOYSA-N C3-thiacarbocyanine cation Chemical compound S1C2=CC=CC=C2[N+](CC)=C1C=CC=C1N(CC)C2=CC=CC=C2S1 JGLWGLKNDHZFAP-UHFFFAOYSA-N 0.000 claims 1
- 229910021193 La 2 O 3 Inorganic materials 0.000 claims 1
- 229910017768 LaF 3 Inorganic materials 0.000 claims 1
- 229910004298 SiO 2 Inorganic materials 0.000 claims 1
- 229910006404 SnO 2 Inorganic materials 0.000 claims 1
- UIMOXRDVWDLOHW-UHFFFAOYSA-N coumarin 481 Chemical compound FC(F)(F)C1=CC(=O)OC2=CC(N(CC)CC)=CC=C21 UIMOXRDVWDLOHW-UHFFFAOYSA-N 0.000 claims 1
- PMPJQLCPEQFEJW-HPKCLRQXSA-L disodium;2-[(e)-2-[4-[4-[(e)-2-(2-sulfonatophenyl)ethenyl]phenyl]phenyl]ethenyl]benzenesulfonate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)C1=CC=CC=C1\C=C\C1=CC=C(C=2C=CC(\C=C\C=3C(=CC=CC=3)S([O-])(=O)=O)=CC=2)C=C1 PMPJQLCPEQFEJW-HPKCLRQXSA-L 0.000 claims 1
- LWBHRFMETSGNFS-UHFFFAOYSA-N phenoxazin-5-ium Chemical compound C1=CC=CC2=NC3=CC=CC=C3[O+]=C21 LWBHRFMETSGNFS-UHFFFAOYSA-N 0.000 claims 1
- 239000000523 sample Substances 0.000 description 108
- 238000010586 diagram Methods 0.000 description 45
- 238000010521 absorption reaction Methods 0.000 description 33
- 230000006870 function Effects 0.000 description 32
- 239000011521 glass Substances 0.000 description 17
- 239000010408 film Substances 0.000 description 14
- 239000002245 particle Substances 0.000 description 14
- 238000005259 measurement Methods 0.000 description 13
- 230000010287 polarization Effects 0.000 description 11
- 239000000975 dye Substances 0.000 description 10
- VYXSBFYARXAAKO-WTKGSRSZSA-N chembl402140 Chemical compound Cl.C1=2C=C(C)C(NCC)=CC=2OC2=C\C(=N/CC)C(C)=CC2=C1C1=CC=CC=C1C(=O)OCC VYXSBFYARXAAKO-WTKGSRSZSA-N 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 239000006059 cover glass Substances 0.000 description 7
- 238000002073 fluorescence micrograph Methods 0.000 description 7
- 230000014509 gene expression Effects 0.000 description 7
- 238000004776 molecular orbital Methods 0.000 description 7
- 210000001747 pupil Anatomy 0.000 description 7
- 230000001629 suppression Effects 0.000 description 7
- 230000005540 biological transmission Effects 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 238000003384 imaging method Methods 0.000 description 6
- 239000011248 coating agent Substances 0.000 description 5
- 238000000576 coating method Methods 0.000 description 5
- 238000007562 laser obscuration time method Methods 0.000 description 5
- ORUIBWPALBXDOA-UHFFFAOYSA-L magnesium fluoride Chemical compound [F-].[F-].[Mg+2] ORUIBWPALBXDOA-UHFFFAOYSA-L 0.000 description 5
- 229910001635 magnesium fluoride Inorganic materials 0.000 description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 5
- 229910052753 mercury Inorganic materials 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- KYQCOXFCLRTKLS-UHFFFAOYSA-N Pyrazine Chemical compound C1=CN=CC=N1 KYQCOXFCLRTKLS-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 4
- 238000002189 fluorescence spectrum Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical compound [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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- G01N21/636—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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JP09792498A JP3350442B2 (ja) | 1998-04-09 | 1998-04-09 | 顕微鏡システム |
PCT/JP1999/001904 WO1999053356A1 (fr) | 1998-04-09 | 1999-04-09 | Systeme de microscope |
DE19980759.0A DE19980759B8 (de) | 1998-04-09 | 1999-04-09 | Mikroskopsystem |
US09/445,389 US6667830B1 (en) | 1998-04-09 | 1999-04-09 | Super-resolution microscope system and method for illumination |
DE19980759T DE19980759T1 (de) | 1998-04-09 | 1999-04-09 | Mikroskopsystem |
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JP09792498A JP3350442B2 (ja) | 1998-04-09 | 1998-04-09 | 顕微鏡システム |
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Cited By (3)
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US8947658B2 (en) | 2011-09-29 | 2015-02-03 | Olympus Corporation | Nonlinear optical microscope and nonlinear optical microscopy |
KR20150024547A (ko) * | 2013-08-27 | 2015-03-09 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
KR20150109854A (ko) * | 2014-03-21 | 2015-10-02 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
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US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
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DE102006021317B3 (de) * | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
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US8017104B2 (en) * | 2007-02-28 | 2011-09-13 | Carestream Health, Inc. | Large stoke shift dye used for optical imaging |
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KR101031779B1 (ko) * | 2008-11-20 | 2011-04-29 | 한국표준과학연구원 | 다중광노출법 기반 초고분해능 리소그래피 장치 및 방법 |
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EP2581775A1 (en) * | 2011-10-14 | 2013-04-17 | Astrium Limited | Resonator Optimisation |
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US11221473B2 (en) * | 2017-04-06 | 2022-01-11 | Clemson University Research Foundation | Pulse-illuminated edge deduction microscopy |
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DE102018132212B4 (de) * | 2018-12-14 | 2020-09-17 | Leica Microsystems Cms Gmbh | Verfahren und Mikroskop zur hochaufgelösten lichtmikroskopischen Abbildung einer Probe |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60131428A (ja) * | 1983-12-21 | 1985-07-13 | Komatsu Ltd | カ−ス分光装置 |
JPS61111530A (ja) * | 1984-11-06 | 1986-05-29 | Canon Inc | 露光装置 |
US4893886A (en) * | 1987-09-17 | 1990-01-16 | American Telephone And Telegraph Company | Non-destructive optical trap for biological particles and method of doing same |
JPH04171415A (ja) * | 1990-11-02 | 1992-06-18 | Nikon Corp | 長焦点深度高分解能照射光学系 |
US5350695A (en) * | 1991-12-05 | 1994-09-27 | Miles Inc. | Methods for the identification and characterization of reticulocytes in whole blood |
US5245619A (en) * | 1992-01-03 | 1993-09-14 | Kronberg James W | Generation of low-divergence laser beams |
JPH05224128A (ja) * | 1992-02-14 | 1993-09-03 | Olympus Optical Co Ltd | 走査型顕微鏡 |
US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
JPH0815156A (ja) * | 1993-06-03 | 1996-01-19 | Hamamatsu Photonics Kk | レーザスキャン光学系及びレーザスキャン光学装置 |
EP0627643B1 (en) * | 1993-06-03 | 1999-05-06 | Hamamatsu Photonics K.K. | Laser scanning optical system using axicon |
DE4331570C2 (de) * | 1993-08-17 | 1996-10-24 | Hell Stefan | Verfahren zum optischen Anregen einer Probe |
US5866911A (en) * | 1994-07-15 | 1999-02-02 | Baer; Stephen C. | Method and apparatus for improving resolution in scanned optical system |
JP3164989B2 (ja) * | 1994-12-28 | 2001-05-14 | 科学技術振興事業団 | 試料観察方法および多波長光光学顕微鏡 |
JP3011633B2 (ja) * | 1995-03-15 | 2000-02-21 | 科学技術振興事業団 | 多波長光光学顕微鏡 |
JP3020453B2 (ja) * | 1996-11-13 | 2000-03-15 | 科学技術振興事業団 | 光学顕微鏡 |
JPH1195120A (ja) * | 1997-09-19 | 1999-04-09 | Olympus Optical Co Ltd | 顕微鏡の観察方法 |
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1998
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1999
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- 1999-04-09 DE DE19980759T patent/DE19980759T1/de active Granted
- 1999-04-09 WO PCT/JP1999/001904 patent/WO1999053356A1/ja active Application Filing
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Cited By (3)
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US8947658B2 (en) | 2011-09-29 | 2015-02-03 | Olympus Corporation | Nonlinear optical microscope and nonlinear optical microscopy |
KR20150024547A (ko) * | 2013-08-27 | 2015-03-09 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
KR20150109854A (ko) * | 2014-03-21 | 2015-10-02 | 동우 화인켐 주식회사 | 광 루미네선스 코팅 조성물 및 이를 이용한 광 루미네선스 필름 |
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JP2001100102A (ja) | 2001-04-13 |
DE19980759T1 (de) | 2001-10-18 |
DE19980759B8 (de) | 2016-12-22 |
US6667830B1 (en) | 2003-12-23 |
WO1999053356A1 (fr) | 1999-10-21 |
DE19980759B3 (de) | 2016-10-06 |
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