JP2020506680A5 - - Google Patents

Info

Publication number

JP2020506680A5

JP2020506680A5 JP2019537378A JP2019537378A JP2020506680A5 JP 2020506680 A5 JP2020506680 A5 JP 2020506680A5 JP 2019537378 A JP2019537378 A JP 2019537378A JP 2019537378 A JP2019537378 A JP 2019537378A JP 2020506680 A5 JP2020506680 A5 JP 2020506680A5

Authority

JP

Japan

Prior art keywords

methylation

dna

sample

nucleic acid

amount

Prior art date

2018-01-26

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

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• 238000007069 methylation reaction Methods 0.000 claims 28
• 230000011987 methylation Effects 0.000 claims 27
• 239000003550 marker Substances 0.000 claims 23
• 108020004414 DNA Proteins 0.000 claims 19
• 239000000523 sample Substances 0.000 claims 17
• 238000000034 method Methods 0.000 claims 16
• 108020004707 nucleic acids Proteins 0.000 claims 13
• 102000039446 nucleic acids Human genes 0.000 claims 13
• 150000007523 nucleic acids Chemical class 0.000 claims 13
• 108090000623 proteins and genes Proteins 0.000 claims 11
• 108091034117 Oligonucleotide Proteins 0.000 claims 10
• 102100033991 E3 ubiquitin-protein ligase DTX1 Human genes 0.000 claims 7
• 101001017463 Homo sapiens E3 ubiquitin-protein ligase DTX1 Proteins 0.000 claims 7
• CLBIEZBAENPDFY-HNXGFDTJSA-N dinophysistoxin 1 Chemical compound C([C@H](O1)[C@H](C)/C=C/[C@H]2CC[C@@]3(CC[C@H]4O[C@@H](C([C@@H](O)[C@@H]4O3)=C)[C@@H](O)C[C@H](C)[C@@H]3[C@@H](CC[C@@]4(O3)[C@@H](CCCO4)C)C)O2)C(C)=C[C@]21O[C@H](C[C@@](C)(O)C(O)=O)CC[C@H]2O CLBIEZBAENPDFY-HNXGFDTJSA-N 0.000 claims 7
• 238000003556 assay Methods 0.000 claims 5
• 239000003153 chemical reaction reagent Substances 0.000 claims 5
• 102100038781 Carbohydrate sulfotransferase 2 Human genes 0.000 claims 4
• 102100022626 Glutamate receptor ionotropic, NMDA 2D Human genes 0.000 claims 4
• 102100032191 Guanine nucleotide exchange factor VAV3 Human genes 0.000 claims 4
• 101000883009 Homo sapiens Carbohydrate sulfotransferase 2 Proteins 0.000 claims 4
• 101000972840 Homo sapiens Glutamate receptor ionotropic, NMDA 2D Proteins 0.000 claims 4
• 101000775742 Homo sapiens Guanine nucleotide exchange factor VAV3 Proteins 0.000 claims 4
• 101001050321 Homo sapiens Junctional adhesion molecule C Proteins 0.000 claims 4
• 101000665140 Homo sapiens Scm-like with four MBT domains protein 2 Proteins 0.000 claims 4
• 101000964764 Homo sapiens Zinc finger protein 568 Proteins 0.000 claims 4
• 101000915607 Homo sapiens Zinc finger protein 671 Proteins 0.000 claims 4
• 102100023429 Junctional adhesion molecule C Human genes 0.000 claims 4
• 102100038691 Scm-like with four MBT domains protein 2 Human genes 0.000 claims 4
• 102100040655 Zinc finger protein 568 Human genes 0.000 claims 4
• 102100028943 Zinc finger protein 671 Human genes 0.000 claims 4
• 102100034269 Ankyrin repeat domain-containing protein 13B Human genes 0.000 claims 3
• 102100023994 Beta-1,3-galactosyltransferase 6 Human genes 0.000 claims 3
• 102100031597 Dedicator of cytokinesis protein 2 Human genes 0.000 claims 3
• 102100023378 Fer-1-like protein 4 Human genes 0.000 claims 3
• 102100040612 Fermitin family homolog 3 Human genes 0.000 claims 3
• 102100030334 Friend leukemia integration 1 transcription factor Human genes 0.000 claims 3
• 101000780147 Homo sapiens Ankyrin repeat domain-containing protein 13B Proteins 0.000 claims 3
• 101000904594 Homo sapiens Beta-1,3-galactosyltransferase 6 Proteins 0.000 claims 3
• 101000866237 Homo sapiens Dedicator of cytokinesis protein 2 Proteins 0.000 claims 3
• 101000907567 Homo sapiens Fer-1-like protein 4 Proteins 0.000 claims 3
• 101000749644 Homo sapiens Fermitin family homolog 3 Proteins 0.000 claims 3
• 101001062996 Homo sapiens Friend leukemia integration 1 transcription factor Proteins 0.000 claims 3
• 101000945411 Homo sapiens Metal transporter CNNM1 Proteins 0.000 claims 3
• 101000782074 Homo sapiens Palmitoyltransferase ZDHHC1 Proteins 0.000 claims 3
• 101000893493 Homo sapiens Protein flightless-1 homolog Proteins 0.000 claims 3
• 101000658628 Homo sapiens Testis-specific Y-encoded-like protein 5 Proteins 0.000 claims 3
• 101000723909 Homo sapiens Zinc finger protein 304 Proteins 0.000 claims 3
• 102100033593 Metal transporter CNNM1 Human genes 0.000 claims 3
• 101710163270 Nuclease Proteins 0.000 claims 3
• 102100036609 Palmitoyltransferase ZDHHC1 Human genes 0.000 claims 3
• 102100034914 Testis-specific Y-encoded-like protein 5 Human genes 0.000 claims 3
• 102100028422 Zinc finger protein 304 Human genes 0.000 claims 3
• 239000000203 mixture Substances 0.000 claims 3
• LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 2
• 108010022366 Carcinoembryonic Antigen Proteins 0.000 claims 2
• 102100025475 Carcinoembryonic antigen-related cell adhesion molecule 5 Human genes 0.000 claims 2
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
• 101001017833 Homo sapiens Leucine-rich repeat-containing protein 4 Proteins 0.000 claims 2
• 101000653634 Homo sapiens T-box transcription factor TBX15 Proteins 0.000 claims 2
• 102100033304 Leucine-rich repeat-containing protein 4 Human genes 0.000 claims 2
• 108091006628 SLC12A8 Proteins 0.000 claims 2
• 102100036751 Solute carrier family 12 member 8 Human genes 0.000 claims 2
• LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 claims 2
• 102100029853 T-box transcription factor TBX15 Human genes 0.000 claims 2
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
• 102100025908 5-oxoprolinase Human genes 0.000 claims 1
• 101150104383 ALOX5AP gene Proteins 0.000 claims 1
• 108020000946 Bacterial DNA Proteins 0.000 claims 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
• 108090000652 Flap endonucleases Proteins 0.000 claims 1
• 101000720962 Homo sapiens 5-oxoprolinase Proteins 0.000 claims 1
• 101000611892 Homo sapiens Platelet-derived growth factor D Proteins 0.000 claims 1
• 101100236114 Mus musculus Lrrfip1 gene Proteins 0.000 claims 1
• 108020004711 Nucleic Acid Probes Proteins 0.000 claims 1
• 102100040682 Platelet-derived growth factor D Human genes 0.000 claims 1
• 101100478275 Schizosaccharomyces pombe (strain 972 / ATCC 24843) spt8 gene Proteins 0.000 claims 1
• QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
• 230000003321 amplification Effects 0.000 claims 1
• 239000011324 bead Substances 0.000 claims 1
• 239000008280 blood Substances 0.000 claims 1
• 210000004369 blood Anatomy 0.000 claims 1
• 210000001072 colon Anatomy 0.000 claims 1
• 208000029742 colonic neoplasm Diseases 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 1
• 230000003247 decreasing effect Effects 0.000 claims 1
• 238000009585 enzyme analysis Methods 0.000 claims 1
• 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 1
• 238000012268 genome sequencing Methods 0.000 claims 1
• 238000004949 mass spectrometry Methods 0.000 claims 1
• 238000007855 methylation-specific PCR Methods 0.000 claims 1
• 238000003199 nucleic acid amplification method Methods 0.000 claims 1
• 239000002853 nucleic acid probe Substances 0.000 claims 1
• 238000003752 polymerase chain reaction Methods 0.000 claims 1
• 238000002331 protein detection Methods 0.000 claims 1
• 102000004169 proteins and genes Human genes 0.000 claims 1
• 238000012175 pyrosequencing Methods 0.000 claims 1
• 239000011541 reaction mixture Substances 0.000 claims 1
• 238000012216 screening Methods 0.000 claims 1
• 238000000926 separation method Methods 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 239000007787 solid Substances 0.000 claims 1