CN110475875B - 通过分析甲基化dna检测结肠瘤形成 - Google Patents

通过分析甲基化dna检测结肠瘤形成 Download PDF

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CN110475875B
CN110475875B CN201880012946.6A CN201880012946A CN110475875B CN 110475875 B CN110475875 B CN 110475875B CN 201880012946 A CN201880012946 A CN 201880012946A CN 110475875 B CN110475875 B CN 110475875B
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methylation
dna
nucleic acid
flap
sequence
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CN110475875A (zh
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H.T.阿拉维
M.W.凯瑟
G.P.利加德
W.R.泰勒
T.J.桑德
A.M.瓦卡罗
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Exact Sciences Corp
Exact Sciences Development Co LLC
Mayo Clinic in Florida
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Exact Sciences Development Co LLC
Mayo Clinic in Florida
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CN201880012946.6A 2017-01-27 2018-01-26 通过分析甲基化dna检测结肠瘤形成 Active CN110475875B (zh)

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PCT/US2018/015535 WO2018140781A1 (en) 2017-01-27 2018-01-26 Detection of colon neoplasia by analysis of methylated dna

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Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009102788A2 (en) 2008-02-15 2009-08-20 Mayo Foundation For Medical Education And Research Detecting neoplasm
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
ES2812753T3 (es) 2014-03-31 2021-03-18 Mayo Found Medical Education & Res Detección de neoplasma colorectal
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
AU2016315924B2 (en) * 2015-08-31 2022-08-25 Exact Sciences Corporation Detecting gastric neoplasm
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
US10370726B2 (en) * 2016-04-14 2019-08-06 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasia
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
WO2017192221A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated dna
EP3488003B1 (en) * 2016-07-19 2023-10-25 Exact Sciences Corporation Nucleic acid control molecules from non-human organisms
ES2902174T3 (es) * 2016-07-19 2022-03-25 Exact Sciences Dev Co Llc ADN de control metilado
CN110475875B (zh) 2017-01-27 2024-06-25 精密科学公司 通过分析甲基化dna检测结肠瘤形成
CN111655869B (zh) 2017-11-30 2024-06-28 梅约医学教育与研究基金会 检测乳腺癌
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
US20220162709A1 (en) * 2019-04-03 2022-05-26 Mayo Foundation For Medical Education And Research Detecting pancreatic ductal adenocarcinoma in plasma
CN109811035A (zh) * 2019-04-11 2019-05-28 中国人民解放军第四军医大学 一种肠道脱落细胞目标基因启动子甲基化检测的方法及试剂盒
US11396679B2 (en) * 2019-05-31 2022-07-26 Universal Diagnostics, S.L. Detection of colorectal cancer
US11001898B2 (en) 2019-05-31 2021-05-11 Universal Diagnostics, S.L. Detection of colorectal cancer
CN110317874A (zh) * 2019-07-19 2019-10-11 江苏元丞生物科技有限公司 Vav3基因甲基化检测试剂盒及其应用
KR20220092561A (ko) 2019-10-31 2022-07-01 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 난소암 검출
US11898199B2 (en) 2019-11-11 2024-02-13 Universal Diagnostics, S.A. Detection of colorectal cancer and/or advanced adenomas
CN112662765A (zh) * 2020-03-17 2021-04-16 博尔诚(北京)科技有限公司 一种检测6种中国高发癌症的探针组合物
CA3173044A1 (en) * 2020-03-20 2021-09-23 Singlera Health Technologies (Shanghai) Ltd. Methods and kits for screening colorectal neoplasm
CN111961721B (zh) * 2020-04-15 2022-06-07 南方医科大学 血浆sfmbt2基因甲基化在结直肠癌中转移预测及动态监测中的应用
CN115605138A (zh) * 2020-04-17 2023-01-13 精密科学公司(Us) 可摄取取样装置
US12467096B2 (en) 2020-05-15 2025-11-11 Universal Diagnostics, S.A. Methods and systems for identifying methylation biomarkers
WO2022002424A1 (en) 2020-06-30 2022-01-06 Universal Diagnostics, S.L. Systems and methods for detection of multiple cancer types
CN112430657B (zh) * 2020-10-27 2022-09-09 哈尔滨医科大学 结直肠癌相关的甲基化标志物以及用于检测结直肠癌的试剂盒
US11821045B2 (en) 2021-04-13 2023-11-21 Geninus Inc. Colorectal cancer-specific methylation biomarkers for diagnosing colorectal cancer
CN113454243B (zh) * 2021-04-23 2025-03-21 华大数极生物科技(深圳)有限公司 用于结直肠癌检测的组合物、试剂盒及应用
US20220349009A1 (en) * 2021-04-23 2022-11-03 Mayo Foundation For Medical Education And Research Detecting esophageal disorders
CN115433778B (zh) * 2021-06-02 2023-06-16 武汉艾米森生命科技有限公司 一种用于检测结直肠癌或结直肠腺瘤相关基因甲基化的检测试剂、试剂盒及其应用
WO2023017001A1 (en) * 2021-08-09 2023-02-16 Epigenomics Ag Improved methods for detecting colorectal cancer
IL293201B2 (en) * 2022-05-22 2023-04-01 Nucleix Ltd Preparations contain a reaction buffer for DNA amplification and sequencing
EP4582561A1 (en) * 2022-09-02 2025-07-09 BGI Genomics Co., Limited Combination of differentially methylated regions, kit, and use thereof
WO2024045160A1 (zh) * 2022-09-02 2024-03-07 深圳华大基因股份有限公司 Oplah基因的差异性甲基化区域、试剂盒和用途
WO2024075020A1 (en) * 2022-10-04 2024-04-11 Epigenomics Ag Multi-analyte diagnostic and prognostic assays for colorectal cancer and uses thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008103763A2 (en) * 2007-02-20 2008-08-28 Sequenom, Inc. Methods and compositions for cancer diagnosis and treatment based on nucleic acid methylation
CN103415625A (zh) * 2010-11-15 2013-11-27 精密科学公司 甲基化分析
WO2014062218A1 (en) * 2012-10-16 2014-04-24 University Of Southern California Colorectal cancer dna methylation markers
WO2015153283A1 (en) * 2014-03-31 2015-10-08 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasm
CN105143465A (zh) * 2013-03-14 2015-12-09 梅奥医学教育和研究基金会 检测赘生物

Family Cites Families (154)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4486530A (en) 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US5288609A (en) 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
US4735214A (en) 1986-09-05 1988-04-05 Berman Irwin R Gastrointestinal diagnostic capsule and method of use
US5359100A (en) 1987-10-15 1994-10-25 Chiron Corporation Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers
US5124246A (en) 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
CA2036946C (en) 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5849481A (en) 1990-07-27 1998-12-15 Chiron Corporation Nucleic acid hybridization assays employing large comb-type branched polynucleotides
CA2088258C (en) 1990-07-27 2004-09-14 Phillip Dan Cook Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
CA2218875C (en) 1991-07-23 2000-11-07 The Research Foundation Of State University Of New York Improvements in the in situ pcr
US6872816B1 (en) 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
CA2122365C (en) 1991-11-26 2010-05-11 Brian Froehler Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5976790A (en) 1992-03-04 1999-11-02 The Regents Of The University Of California Comparative Genomic Hybridization (CGH)
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
EP0691980B1 (en) 1993-03-30 1997-06-04 Sanofi 7-deazapurine modified oligonucleotides
AU6632094A (en) 1993-04-19 1994-11-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
SE501439C2 (sv) 1993-06-22 1995-02-13 Pharmacia Lkb Biotech Sätt och anordning för analys av polynukleotidsekvenser
WO1995014106A2 (en) 1993-11-17 1995-05-26 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
DE69417918T2 (de) 1993-11-30 2000-01-05 Mcgill University, Montreal Dna methyltransferase inhibierung
CA2188660C (en) 1994-04-25 2005-01-18 Richard G. H. Cotton Detection of mutation by resolvase cleavage
US5851770A (en) 1994-04-25 1998-12-22 Variagenics, Inc. Detection of mismatches by resolvase cleavage using a magnetic bead support
EP1260593A3 (en) 1994-12-23 2003-12-03 Dade Behring Inc. Detection of nucleic acids by nuclease-catalyzed product formation
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1997023647A1 (en) 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Homogeneous amplification and detection of nucleic acids
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US7256020B2 (en) 1996-11-29 2007-08-14 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
DE69738687D1 (de) 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US6017704A (en) 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
AU7829398A (en) 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
US6013459A (en) 1997-06-12 2000-01-11 Clinical Micro Sensors, Inc. Detection of analytes using reorganization energy
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6063573A (en) 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
DE69902996T2 (de) 1998-02-04 2003-04-30 Variagenics, Inc. Techniken zu detektion von fehlpaarungen
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
MXPA03001834A (es) 2000-09-01 2003-08-01 Epigenomics Ag Metodo para determinar el grado de metilacion de determinadas citocinas en adn genomico en el contexto de secuencia 5'-cpg-3'.
AU2001297524A1 (en) 2000-11-15 2002-09-19 Third Wave Technologies, Inc. Fen endonucleases
US20040014067A1 (en) 2001-10-12 2004-01-22 Third Wave Technologies, Inc. Amplification methods and compositions
US7482118B2 (en) * 2001-11-15 2009-01-27 Third Wave Technologies, Inc. Endonuclease-substrate complexes
EP1497462A4 (en) 2002-03-07 2007-11-07 Univ Johns Hopkins Med GENOMIC SCREEN ON EPIGENETIC MUTED CANCER ASSOCIATED GENES
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
EP2031070B1 (en) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplex amplification of polynucleotides
AU2004270220B2 (en) 2003-09-05 2009-03-05 The Chinese University Of Hong Kong Method for non-invasive prenatal diagnosis
CN102517389A (zh) 2003-10-09 2012-06-27 Epi基因组股份公司 改进的dna亚硫酸氢盐转化
KR100880516B1 (ko) 2003-10-16 2009-01-28 써드 웨이브 테크놀로지스, 아이앤씨. 체액에서 핵산의 직접 검출법
WO2005042713A2 (en) * 2003-10-28 2005-05-12 The Johns Hopkins University Quantitative multiplex methylation-specific pcr
AU2005200670B2 (en) 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
AU2005230936B2 (en) 2004-03-26 2010-08-05 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
JP2008502890A (ja) * 2004-06-18 2008-01-31 エフ.ホフマン−ラ ロシュ アーゲー 結腸直腸癌のマーカーとしてのcbp2の使用
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2006050499A2 (en) 2004-11-03 2006-05-11 Third Wave Technologies, Inc. Single step detection assay
AU2005300688B2 (en) 2004-11-03 2012-02-02 Almac Diagnostics Limited Transcriptome microarray technology and methods of using the same
EP1844162B1 (en) 2005-02-01 2014-10-15 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
US20060171952A1 (en) 2005-02-02 2006-08-03 Mather Jennie P JAM-3 and antibodies that bind thereto
DE602005009324D1 (de) 2005-04-06 2008-10-09 Maurice Stroun Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf
PT1871912E (pt) 2005-04-15 2012-05-25 Epigenomics Ag Método para determinar metilação de adn em amostras de sangue ou urina
KR101515821B1 (ko) 2005-09-01 2015-04-30 오스다이어그나스틱스 피티와이 엘티디. 핵산의 증폭, 정량분석 및 동정 방법
TR201910868T4 (tr) 2006-02-02 2019-08-21 Univ Leland Stanford Junior Dijital analizle invazif olmayan fetal genetik tarama.
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US20080026390A1 (en) 2006-06-14 2008-01-31 Roland Stoughton Diagnosis of Fetal Abnormalities by Comparative Genomic Hybridization Analysis
GB0700374D0 (en) 2007-01-09 2007-02-14 Oncomethylome Sciences S A NDRG family methylation markers
US20090203011A1 (en) 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
CA2676227A1 (en) 2007-02-12 2008-08-21 The Johns Hopkins University Early detection and prognosis of colon cancers
US20100075334A1 (en) 2007-04-16 2010-03-25 Yong Sung Kim Methylation biomarker for early detection of gastric cancer
PL2479289T3 (pl) 2007-06-08 2016-10-31 Sposób analizy metylacji
CA2693098C (fr) * 2007-07-19 2019-06-18 Yasemin Ataman-Onal Procede de dosage de la proteine de liaison hepatique aux acides gras, de l'antigene carcino-embryonnaire, et de l'antigene carbohydrate 19-9 pour le diagnostic in vitro du cancercolorectal
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
WO2009102788A2 (en) 2008-02-15 2009-08-20 Mayo Foundation For Medical Education And Research Detecting neoplasm
US8256177B2 (en) 2008-03-12 2012-09-04 Masonite Corporation Impact resistant door skin, door including the same, and method of manufacturing an impact resistant door skin from a pre-formed door skin
AU2009225835B2 (en) 2008-03-15 2013-02-14 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
US20110318738A1 (en) 2008-12-23 2011-12-29 University Of Utah Research Foundation Identification and regulation of a novel dna demethylase system
EP2233590A1 (en) 2009-01-28 2010-09-29 AIT Austrian Institute of Technology GmbH Methylation assay
US20120164238A1 (en) 2009-02-03 2012-06-28 Mdxhealth Sa Methods of detecting colorectal cancer
US20110287424A1 (en) 2009-03-27 2011-11-24 Life Technologies Corporation Methylation-specific competitive allele-specific taqman polymerase chain reaction (cast-pcr)
GB0920014D0 (en) 2009-11-13 2009-12-30 Medical Res Council Cell sampling device
WO2011063274A2 (en) 2009-11-23 2011-05-26 Genomic Health, Inc. Methods to predict clinical outcome of cancer
WO2011119934A2 (en) 2010-03-26 2011-09-29 Mayo Foundation For Medical Education And Research Methods and materials for detecting colorectal neoplasm
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
WO2012106525A2 (en) 2011-02-02 2012-08-09 Exact Sciences Corporation Digital sequence analysis of dna methylation
US8808990B2 (en) 2011-05-12 2014-08-19 Exact Sciences Corporation Serial isolation of multiple DNA targets from stool
CN103649298A (zh) 2011-05-12 2014-03-19 精密科学公司 核酸的分离
US9127318B2 (en) 2011-10-18 2015-09-08 Exact Sciences Corporation Multiplexed KRAS mutation detection assay
WO2013070950A1 (en) 2011-11-08 2013-05-16 University Of Southern California Identification of a dna methylation marker for blood-based detection of ovarian cancer
US9546403B1 (en) 2011-12-14 2017-01-17 University Of Utah Research Foundation Substrate for methylated DNA testing
JP2015503356A (ja) 2012-01-06 2015-02-02 バイオミクス, インコーポレイテッドViomics, Inc. 末梢血中で、がんによって変化したrnaを検出するシステムおよび方法
AU2013215159B2 (en) 2012-01-30 2018-07-12 Exact Sciences Corporation Modification of DNA on magnetic beads
EP2828405B1 (en) 2012-03-20 2017-06-28 Exact Sciences Corporation Marker panel for detecting cancer
KR102393608B1 (ko) 2012-09-04 2022-05-03 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US9212392B2 (en) 2012-09-25 2015-12-15 Exact Sciences Corporation Normalization of polymerase activity
US10648035B2 (en) 2012-11-26 2020-05-12 The Johns Hopkins University Methods and compositions for diagnosing and treating gastric cancer
WO2014160117A1 (en) 2013-03-14 2014-10-02 Abbott Molecular Inc. Multiplex methylation-specific amplification systems and methods
US10253358B2 (en) 2013-11-04 2019-04-09 Exact Sciences Development Company, Llc Multiple-control calibrators for DNA quantitation
EP3084004A4 (en) 2013-12-19 2017-08-16 Exact Sciences Corporation Synthetic nucleic acid control molecules
US20160010081A1 (en) 2014-07-11 2016-01-14 Exact Sciences Corporation Systems, methods and kits for extracting nucleic acid from digestive fluid
US10292687B2 (en) 2014-08-15 2019-05-21 Covidien Lp Designs and methods to facilitate swallowing of a tethered cell collection device
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
CN113981057A (zh) 2014-12-12 2022-01-28 精密科学公司 用于进行甲基化检测测定的组合物和方法
EP3230476B1 (en) * 2014-12-12 2020-02-05 Exact Sciences Development Company, LLC Zdhhc1 for normalizing methylation detection assays
EP3239302A4 (en) 2014-12-26 2018-05-23 Peking University Method for detecting differentially methylated cpg islands associated with abnormal state of human body
US10030272B2 (en) * 2015-02-27 2018-07-24 Mayo Foundation For Medical Education And Research Detecting gastrointestinal neoplasms
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
AU2016315924B2 (en) 2015-08-31 2022-08-25 Exact Sciences Corporation Detecting gastric neoplasm
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
EP3408407B1 (en) 2016-01-29 2021-01-06 Epigenomics AG Methods for detecting cpg methylation of tumor-derived dna in blood samples
US10370726B2 (en) 2016-04-14 2019-08-06 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasia
EP4488686A3 (en) 2016-04-14 2025-04-30 Guardant Health, Inc. Methods for early detection of cancer
AU2017250663B2 (en) 2016-04-14 2023-06-15 Mayo Foundation For Medical Education And Research Detecting pancreatic high-grade dysplasia
WO2017192221A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by analysis of methylated dna
US20170321286A1 (en) 2016-05-05 2017-11-09 Exact Sciences Corporation Detection of lung neoplasia by amplification of rna sequences
CN109715830A (zh) 2016-06-21 2019-05-03 威斯塔解剖学和生物学研究所 用于使用基因表达谱诊断肺癌的组合物和方法
ES2902174T3 (es) 2016-07-19 2022-03-25 Exact Sciences Dev Co Llc ADN de control metilado
WO2018045322A1 (en) 2016-09-02 2018-03-08 Mayo Foundation For Medical Education And Research Detecting hepatocellular carcinoma
CN110475875B (zh) 2017-01-27 2024-06-25 精密科学公司 通过分析甲基化dna检测结肠瘤形成
CA3054836A1 (en) 2017-02-28 2018-09-07 Mayo Foundation For Medical Education And Research Detecting prostate cancer
CN111655869B (zh) 2017-11-30 2024-06-28 梅约医学教育与研究基金会 检测乳腺癌
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
MX2021005963A (es) 2018-11-27 2021-08-24 Exact Sciences Dev Co Llc Caracterizacion de adn metilado, arn y proteinas en la deteccion de neoplasia pulmonar.
CN113557308B (zh) 2019-01-24 2024-08-16 梅约医学教育与研究基金会 检测子宫内膜癌
US20220162709A1 (en) 2019-04-03 2022-05-26 Mayo Foundation For Medical Education And Research Detecting pancreatic ductal adenocarcinoma in plasma
WO2021041726A1 (en) 2019-08-27 2021-03-04 Exact Sciences Development Company, Llc Characterizing methylated dna, rna, and proteins in subjects suspected of having lung neoplasia
CN114651070A (zh) 2019-09-16 2022-06-21 精密科学公司 结构和温度依赖性瓣状核酸内切酶底物
EP4045913A4 (en) 2019-10-18 2023-11-08 Exact Sciences Corporation Stool ANTIGEN TEST WITH MULTIPLE ANALYTES
KR20220092561A (ko) 2019-10-31 2022-07-01 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 난소암 검출
CN115605138A (zh) 2020-04-17 2023-01-13 精密科学公司(Us) 可摄取取样装置
JP2023524750A (ja) 2020-05-04 2023-06-13 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ メラノーマの検出
US20230167506A1 (en) 2020-05-04 2023-06-01 Mayo Foundation For Medical Education And Research Detecting pancreatic neuroendocrine tumors
EP4200445A4 (en) 2020-08-19 2025-08-27 Mayo Found Medical Education & Res DETECTION OF NON-HODGKIN'S LYMPHOMA
AU2022213409A1 (en) 2021-01-29 2023-08-17 Exact Sciences Corporation Detecting the presence or absence of multiple types of cancer
JP2024509851A (ja) 2021-03-05 2024-03-05 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 子宮頸癌の検出
US20220349009A1 (en) 2021-04-23 2022-11-03 Mayo Foundation For Medical Education And Research Detecting esophageal disorders
CN118574943A (zh) 2021-11-05 2024-08-30 梅奥医疗教育与研究基金会 用于检测口咽癌的组合物和方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008103763A2 (en) * 2007-02-20 2008-08-28 Sequenom, Inc. Methods and compositions for cancer diagnosis and treatment based on nucleic acid methylation
CN103415625A (zh) * 2010-11-15 2013-11-27 精密科学公司 甲基化分析
WO2014062218A1 (en) * 2012-10-16 2014-04-24 University Of Southern California Colorectal cancer dna methylation markers
CN105143465A (zh) * 2013-03-14 2015-12-09 梅奥医学教育和研究基金会 检测赘生物
WO2015153283A1 (en) * 2014-03-31 2015-10-08 Mayo Foundation For Medical Education And Research Detecting colorectal neoplasm

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