JP2019523407A5 - - Google Patents

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JP2019523407A5
JP2019523407A5 JP2019502253A JP2019502253A JP2019523407A5 JP 2019523407 A5 JP2019523407 A5 JP 2019523407A5 JP 2019502253 A JP2019502253 A JP 2019502253A JP 2019502253 A JP2019502253 A JP 2019502253A JP 2019523407 A5 JP2019523407 A5 JP 2019523407A5
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composition according
fluorescence
binding polypeptide
flow cytometry
detection
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JP2019502253A 2016-07-21 2017-07-21 Ｆｃ結合能を有する融合タンパク質を含む細胞外小胞の使用 Active JP7140743B2 (ja)

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JP2022084613A JP2022117998A (ja) 2016-07-21 2022-05-24 Ｆｃ結合能を有する融合タンパク質を含む細胞外小胞の使用

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GB1612643.5A GB2552473A (en) 2016-07-21 2016-07-21 Surface decoration of extracellular vesicles
GB1612643.5 2016-07-21
PCT/EP2017/068486 WO2018015539A1 (en) 2016-07-21 2017-07-21 Uses of extracellular vesicle comprising a fusion protein having fc binding capacity

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JP2019523407A JP2019523407A (ja) 2019-08-22
JP2019523407A5 true JP2019523407A5 (https=) 2022-06-10
JP7140743B2 JP7140743B2 (ja) 2022-09-21

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JP2019502253A Active JP7140743B2 (ja) 2016-07-21 2017-07-21 Ｆｃ結合能を有する融合タンパク質を含む細胞外小胞の使用
JP2019502697A Active JP7254016B2 (ja) 2016-07-21 2017-07-21 Ｆｃ結合能力を有する融合タンパク質を含む細胞外小胞
JP2022084613A Pending JP2022117998A (ja) 2016-07-21 2022-05-24 Ｆｃ結合能を有する融合タンパク質を含む細胞外小胞の使用
JP2022192884A Withdrawn JP2023026440A (ja) 2016-07-21 2022-12-01 Ｆｃ結合能力を有する融合タンパク質を含む細胞外小胞

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JP2022084613A Pending JP2022117998A (ja) 2016-07-21 2022-05-24 Ｆｃ結合能を有する融合タンパク質を含む細胞外小胞の使用
JP2022192884A Withdrawn JP2023026440A (ja) 2016-07-21 2022-12-01 Ｆｃ結合能力を有する融合タンパク質を含む細胞外小胞

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US (3) US11236143B2 (https=)
EP (3) EP3487528B1 (https=)
JP (4) JP7140743B2 (https=)
KR (2) KR20190032479A (https=)
CN (2) CN109689098A (https=)
AU (2) AU2017299214A1 (https=)
BR (2) BR112019001099A2 (https=)
CA (2) CA3031183A1 (https=)
GB (1) GB2552473A (https=)
MX (2) MX2019000863A (https=)
SG (4) SG11201811152YA (https=)
WO (2) WO2018015539A1 (https=)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112013008697A2 (pt) 2010-09-24 2016-06-21 Massachusetts Inst Technology géis nanoestruturados capazes de liberação controlada de agentes encapsulados
GB2552774A (en) * 2016-07-12 2018-02-14 Evox Therapeutics Ltd EV-Mediated delivery of binding protein-small molecule conjugates
GB2552473A (en) * 2016-07-21 2018-01-31 Evox Therapeutics Ltd Surface decoration of extracellular vesicles
US11020410B2 (en) 2017-02-03 2021-06-01 The Brigham And Women's Hospital, Inc. Self-assembled gels formed with anti-retroviral drugs, prodrugs thereof, and pharmaceutical uses thereof
GB201702863D0 (en) * 2017-02-22 2017-04-05 Evox Therapeutics Ltd Improved loading of EVs with therapeutic proteins
WO2019032628A1 (en) 2017-08-07 2019-02-14 The Regents Of The University Of California GENERATING PLATFORM FOR SAFE CELLULAR THERAPEUTIC AGENTS
GB201717446D0 (en) * 2017-10-24 2017-12-06 Evox Therapeutics Ltd Affinity purification of engineering extracellular vesicles
GB201804291D0 (en) * 2018-03-16 2018-05-02 Evox Therapeutics Ltd Cell-mediated exosome delivery
JP2021520352A (ja) 2018-04-04 2021-08-19 アリヴィオ セラピューティクス， インコーポレイテッド 生物製剤の制御送達用の自己組織化ゲルおよびその製造方法
TWI770396B (zh) * 2018-06-28 2022-07-11 中央研究院 合成多肽及其用途
KR102917499B1 (ko) 2018-07-20 2026-01-26 더 보드 오브 트러스티스 오브 더 리랜드 스탠포드 쥬니어 유니버시티 백혈병 억제 인자 활성을 억제하기 위한 가용성 폴리펩타이드 및 이의 사용 방법
GB201812992D0 (en) 2018-08-09 2018-09-26 Univ Greenwich Liposomes
SG11202100864VA (en) 2018-08-14 2021-02-25 Evercyte Gmbh Target-specific extracellular vesicles
CN108998402B (zh) * 2018-08-28 2020-05-29 江南大学 一种重组枯草芽孢杆菌及其构建方法与应用
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
BR112021007286A2 (pt) 2018-10-19 2021-07-27 Ohio State Innovation Foundation células furtivas terapêuticas nanoprojetadas
CN113286826A (zh) * 2018-10-19 2021-08-20 俄亥俄州国家创新基金会 用于针对髓源性抑制细胞的靶向治疗的细胞外囊泡
BR112021007285A2 (pt) 2018-10-19 2021-07-27 Ohio State Innovation Foundation nanocarreadores para terapia de inflamação pulmonar
JP7659499B2 (ja) * 2018-10-25 2025-04-09 ネクセル カンパニー，リミテッド 線維症を治療又は予防するための組成物及び方法
CN113272426A (zh) * 2018-11-16 2021-08-17 医福斯治疗有限公司 用于尿素循环蛋白和核酸的替代的细胞外囊泡
BR112021012647A2 (pt) * 2018-12-27 2021-09-14 Akeso Biopharma, Inc Anticorpo, polipeptídeo isolado, polinucleotídeo isolado, vetor, célula hospedeira, métodos para preparar o anticorpo e para a prevenção e/ou tratamento e/ou tratamento adjuvante e/ou diagnóstico de uma doença, conjugado de anticorpo, anticorpo multiespecífico, proteína de fusão, composição farmacêutica, kit, usos do anticorpo, método in vivo ou in vitro, célula de hibridoma e anticorpo monoclonal
CA3128386A1 (en) * 2019-02-04 2020-08-13 Codiak Biosciences, Inc. Membrane protein scaffolds for exosome engineering
EP3965829A4 (en) * 2019-05-06 2023-05-24 Malcolm, Thomas ADAPTED HYPOIMMUNE NANOVESICULAR DELIVERY SYSTEM FOR CANCER TUMORS
WO2020252455A1 (en) * 2019-06-13 2020-12-17 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
WO2020249757A1 (en) * 2019-06-14 2020-12-17 Philogen S.P.A Immunoconjugates comprising a single chain diabody and interleukin-15 or interleukin-15 and a sushi domain of interleukin-15 receptor alpha
EP3986429A4 (en) * 2019-06-21 2023-07-19 Entelexo Biotherapeutics Inc. PLATFORMS, COMPOSITIONS, AND METHODS OF ADMINISTRATION OF THERAPEUTICS
US20210093567A1 (en) * 2019-08-06 2021-04-01 Ohio State Innovation Foundation Therapeutic extracellular vesicles
WO2021045501A1 (ko) * 2019-09-02 2021-03-11 경북대학교 산학협력단 Il-2 표면 발현 세포외 소포체를 유효성분으로 포함하는 암 질환 예방 또는 치료용 조성물
CN112941028A (zh) * 2019-12-09 2021-06-11 上海细胞治疗集团有限公司 纳米抗体基因修饰的间充质干细胞及其制备方法与应用
JP2023512562A (ja) 2020-02-05 2023-03-27 ダイアデム バイオセラピューティクス インコーポレイテッド 人工シナプス
WO2021211460A1 (en) * 2020-04-13 2021-10-21 City Of Hope Cell-receptor targeted exosomes
CN111544453B (zh) * 2020-04-27 2021-01-22 高连如 一种包含三种血管再生属性的混合干细胞注射液及其制备方法
CN111560352B (zh) * 2020-06-02 2023-03-24 新疆农业大学 一种病毒样囊泡及其制备方法和应用
CN111905105B (zh) * 2020-07-02 2023-05-05 华南师范大学 一种用于癌症靶向治疗的蛋白类纳米药物及其制备方法
CN112111513A (zh) * 2020-07-17 2020-12-22 深圳市第二人民医院 基于外泌体平台的新冠病毒疫苗制备方法
CA3189601A1 (en) 2020-07-24 2022-01-27 The General Hospital Corporation Enhanced virus-like particles and methods of use thereof for delivery to cells
CN113967254B (zh) * 2020-08-04 2022-08-12 华南理工大学 用于多特异性抗体递送的细胞膜包覆型纳米适配子及应用
JP2023536665A (ja) * 2020-08-05 2023-08-28 オハイオ・ステイト・イノベーション・ファウンデーション アダプターポリペプチドおよびその使用方法
CN112285195B (zh) * 2020-10-27 2021-08-10 江南大学 一种牛奶外泌体的特征性糖蛋白标志物和牛奶外泌体的特征性标志物分离方法
WO2022149779A1 (ko) * 2021-01-11 2022-07-14 주식회사 엑소코바이오 과발현된 Fc 수용체 또는 이의 일부를 포함하는 엑소좀 및 이의 제조방법
KR102252325B1 (ko) * 2021-01-14 2021-05-14 이장호 태아 유래 태초 면역글로불린을 포함하는 세포외소포의 제조방법
WO2022158836A1 (ko) * 2021-01-21 2022-07-28 주식회사 엑소코바이오 목적 단백질 또는 펩타이드가 융합가능한 Fc 수용체 또는 이의 일부를 포함하는 재조합 엑소좀 및 이의 용도
US20240218046A1 (en) * 2021-04-14 2024-07-04 Anjarium Biosciences Ag Fc-derived polypeptides
JP2024514157A (ja) * 2021-04-14 2024-03-28 アンジャリウム バイオサイエンシズ エージー 薬物送達のためのペプチド、ナノ小胞、及びその使用
CA3217654A1 (en) * 2021-04-23 2022-10-27 Spot Biosystems Ltd. Extracellular vesicles loaded with biomolecules
GB202107182D0 (en) * 2021-05-19 2021-06-30 Evox Therapeutics Ltd Nanoparticle delivery system for production of engineered extracellular vesicles
EP4355304A4 (en) * 2021-06-17 2025-04-30 Malcolm, Thomas PERSONALIZED HYPOIMMUNE NANOVESICULAR DELIVERY SYSTEMS FOR CANCERS, INHERITED DISEASES AND INFECTIOUS DISEASES
CN115770302A (zh) * 2021-08-13 2023-03-10 南京大学 一种rna递送系统及其应用
CN113599516B (zh) * 2021-08-16 2022-02-18 上海蒙彼利生物技术有限公司 制备外泌体方法及其药物组合物在组织修复中的应用
KR102907010B1 (ko) * 2021-09-08 2026-02-02 주식회사 꿈랩 핵산분자의 안정적인 전달을 위한 플라스미드 플랫폼
CN113897387B (zh) * 2021-10-09 2023-09-05 深圳市汉科生命工程有限公司 一种具有促进毛发再生功能的基因重组msc及其外泌体样纳米材料的制备方法和应用
DK4190349T3 (da) * 2021-12-03 2025-04-14 Univ China Medical Produktion af exosomer og anvendelse heraf
CN114107253B (zh) * 2021-12-17 2024-03-15 复旦大学附属华山医院 一种利用工程细胞进行基因编辑的系统及方法
CN114236113B (zh) * 2021-12-21 2023-07-21 南京农业大学 一种2,4-滴的即用型免疫传感器
WO2023133425A1 (en) * 2022-01-04 2023-07-13 The Broad Institute, Inc. Compositions and methods for delivering cargo to a target cell
EP4505178A4 (en) * 2022-04-07 2026-04-29 Univ Michigan Regents METHODS AND DEVICES FOR SCREENING EXTRACELLULAR VESICLES
WO2024064748A2 (en) * 2022-09-20 2024-03-28 North Carolina State University Compositions and methods related to exosomal delivery of therapeutic agents
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用
CN117802045A (zh) * 2022-09-30 2024-04-02 北京恩康医药有限公司 一种工程化细胞外囊泡的构建及其应用
CN115873905A (zh) * 2022-11-15 2023-03-31 重庆医科大学附属儿童医院 一种gd2纳米抗体靶向修饰的类外泌体的新型药物载体的制备方法
KR102929157B1 (ko) 2023-03-08 2026-02-23 차이나 메디칼 유니버시티 엑소좀의 생산 및 이의 용도
KR20240158824A (ko) * 2023-04-26 2024-11-05 (주)인엑소플랫 면역글로불린 Fc 영역 또는 이의 변이체를 포함하는 재조합 세포외 소포체 및 이를 이용한 세포외 소포체의 분리 방법
WO2024225847A1 (ko) * 2023-04-28 2024-10-31 연세대학교 산학협력단 유방암 유래 엑소좀 또는 세포외 소포체 분리 방법
GB202307239D0 (en) 2023-05-15 2023-06-28 Evox Therapeutics Ltd Gene therapies for Phenylketonuria
CN119120376A (zh) * 2023-07-10 2024-12-13 浙江大学医学院附属第一医院 一种用于清除致病抗体的嵌合基因工程化纳米囊泡及其制备方法、应用
WO2025068572A2 (en) 2023-09-29 2025-04-03 Evox Therapeutics Limited Polypeptides comprising inteins for engineered evs
WO2025102257A1 (zh) * 2023-11-15 2025-05-22 深圳先进技术研究院 一种靶向子宫工程化细胞外囊泡的制备方法及其应用
CN121100135A (zh) * 2024-04-18 2025-12-09 苏州鲲石一号生物科技有限公司 Gas6融合蛋白及其制备方法与应用
CN118725135B (zh) * 2024-09-02 2025-02-07 中国科学院苏州纳米技术与纳米仿生研究所 外泌体的改造方法
CN119019494B (zh) * 2024-10-28 2025-02-07 广州飞来爱生命科技有限公司 一种治疗心梗的脐带间充质干细胞及其制备方法和应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999059643A2 (en) * 1998-05-20 1999-11-25 Sdg, Inc. Liposomal delivery complex
US20040241176A1 (en) * 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
US20070128117A1 (en) * 2003-02-04 2007-06-07 Bracco International B.V. Ultrasound contrast agents and process for the preparation thereof
JP2007295929A (ja) * 2006-04-05 2007-11-15 Geno Membrane:Kk イヌＢｓｅｐ遺伝子
CA2747020A1 (en) * 2007-08-30 2009-03-05 Virexx Medical Corp. Antigenic compositions and use of same in the targeted delivery of nucleic acids
JP2010540466A (ja) 2007-09-24 2010-12-24 ザ ユニバーシティ オブ クイーンズランド 分子送達ベシクル
US20130053426A1 (en) 2009-04-17 2013-02-28 Yiqi Seow Composition For Delivery Of Genetic Material
ES2623912T3 (es) * 2010-12-23 2017-07-12 Janssen Biotech, Inc. Mutantes FC de anticuerpo activo resistente a proteasa
CA2827443C (en) 2011-02-15 2021-07-06 Vaxiion Therapeutics, Inc. Therapeutic compositions and methods for antibody and fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
GB201121069D0 (en) 2011-12-07 2012-01-18 Isis Innovation Delivery system
GB201121070D0 (en) 2011-12-07 2012-01-18 Isis Innovation composition for delivery of biotherapeutics
CA2910802A1 (en) 2013-04-12 2014-10-16 Samir El Andaloussi Therapeutic delivery vesicles
WO2015002956A1 (en) 2013-07-01 2015-01-08 Ohio State Innovation Foundation Exosome delivery system
US10538570B2 (en) * 2013-09-30 2020-01-21 Northwestern University Targeted and modular exosome loading system
EP3057662A4 (en) 2013-10-17 2017-03-29 Children's Hospital Los Angeles Antibody dependent exosome therapy
WO2015143113A1 (en) * 2014-03-20 2015-09-24 Barb Ariel Cohen Preparations of derived extracellular vesicles, assays, and methods to modify therapeutic outcomes using such preparations
GB2552473A (en) * 2016-07-21 2018-01-31 Evox Therapeutics Ltd Surface decoration of extracellular vesicles

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