JP2019516378A5 - - Google Patents
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- Publication number
- JP2019516378A5 JP2019516378A5 JP2018560571A JP2018560571A JP2019516378A5 JP 2019516378 A5 JP2019516378 A5 JP 2019516378A5 JP 2018560571 A JP2018560571 A JP 2018560571A JP 2018560571 A JP2018560571 A JP 2018560571A JP 2019516378 A5 JP2019516378 A5 JP 2019516378A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- host cell
- component
- exogenous
- landing pad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091033319 polynucleotide Proteins 0.000 claims 36
- 102000040430 polynucleotide Human genes 0.000 claims 36
- 239000002157 polynucleotide Substances 0.000 claims 36
- 210000004027 cell Anatomy 0.000 claims 22
- 238000000034 method Methods 0.000 claims 15
- 239000002773 nucleotide Substances 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 238000011144 upstream manufacturing Methods 0.000 claims 9
- 230000006801 homologous recombination Effects 0.000 claims 7
- 238000002744 homologous recombination Methods 0.000 claims 7
- 101710163270 Nuclease Proteins 0.000 claims 6
- 108020004414 DNA Proteins 0.000 claims 4
- 239000012212 insulator Substances 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 230000002538 fungal effect Effects 0.000 claims 2
- 238000001727 in vivo Methods 0.000 claims 2
- 230000004568 DNA-binding Effects 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 210000004102 animal cell Anatomy 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000007935 neutral effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662338412P | 2016-05-18 | 2016-05-18 | |
| US62/338,412 | 2016-05-18 | ||
| PCT/US2017/033369 WO2017201311A2 (en) | 2016-05-18 | 2017-05-18 | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019516378A JP2019516378A (ja) | 2019-06-20 |
| JP2019516378A5 true JP2019516378A5 (enExample) | 2020-06-18 |
| JP7093310B2 JP7093310B2 (ja) | 2022-06-29 |
Family
ID=59014740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018560571A Active JP7093310B2 (ja) | 2016-05-18 | 2017-05-18 | 外因性ランディングパッドへの核酸のゲノム組込みのための組成物および方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US11293033B2 (enExample) |
| EP (2) | EP4219731A3 (enExample) |
| JP (1) | JP7093310B2 (enExample) |
| WO (1) | WO2017201311A2 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| WO2016073990A2 (en) | 2014-11-07 | 2016-05-12 | Editas Medicine, Inc. | Methods for improving crispr/cas-mediated genome-editing |
| AU2016326711B2 (en) | 2015-09-24 | 2022-11-03 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/Cas-mediated genome editing |
| EP3433363A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| KR101839109B1 (ko) * | 2016-09-22 | 2018-03-15 | ㈜프론트바이오 | 퍼플콘 추출물을 함유하는 피부 질환의 예방 또는 치료용 약제학적 조성물 |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| EP4613765A1 (en) | 2019-07-11 | 2025-09-10 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| AU2021206008A1 (en) * | 2020-01-07 | 2022-07-28 | Limmatech Biologics Ag | Engineered Leishmania cells |
| US11739359B2 (en) * | 2020-05-01 | 2023-08-29 | Microsoft Technology Licensing, Llc | Universal template strands for enzymatic polynucleotide synthesis |
| WO2021253372A1 (zh) * | 2020-06-19 | 2021-12-23 | 深圳华大智造科技有限公司 | 一种高兼容性的PCR-free建库和测序方法 |
| EP4217500A4 (en) * | 2020-09-25 | 2024-10-16 | Bondi Bio Pty Ltd | STANDARDIZED CYANOBACTERIA STRAIN CONSTRUCTION |
| CA3229003A1 (en) * | 2021-08-25 | 2023-03-02 | Kothai Nachiar Devi PARTHIBAN | Preparation of libraries of protein variants expressed in eukaryotic cells |
| CN114134091B (zh) * | 2021-11-08 | 2024-04-12 | 中国科学院武汉病毒研究所 | 细菌基因组绝缘位点的鉴定方法及应用 |
| WO2024050054A1 (en) * | 2022-08-31 | 2024-03-07 | The Regents Of The University Of California | High throughput characterization of bacterial promoters from their host environments |
| WO2024076688A2 (en) * | 2022-10-05 | 2024-04-11 | The Regents Of The University Of California | Synthetic genomic safe harbors and methods thereof |
| CN120731271A (zh) * | 2023-04-21 | 2025-09-30 | 诺维信公司 | 多拷贝宿主细胞的生成 |
| WO2025119959A1 (en) * | 2023-12-05 | 2025-06-12 | F. Hoffmann-La Roche Ag | Monitoring populations of cells producing biomolecules |
Family Cites Families (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5272065A (en) | 1983-10-20 | 1993-12-21 | Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
| US4873192A (en) | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
| US6140466A (en) | 1994-01-18 | 2000-10-31 | The Scripps Research Institute | Zinc finger protein derivatives and methods therefor |
| DE69623057T2 (de) | 1995-06-07 | 2003-03-27 | Invitrogen Corp., Carlsbad | Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte |
| JP4206154B2 (ja) | 1997-11-13 | 2009-01-07 | 大日本住友製薬株式会社 | 変異型loxP配列とその応用 |
| US5929301A (en) | 1997-11-18 | 1999-07-27 | Pioneer Hi-Bred International | Nucleic acid sequence encoding FLP recombinase |
| PT1032680E (pt) | 1997-11-18 | 2006-05-31 | Pioneer Hi Bred Int | Um novo metodo para a integracao de adn estranho em genomas eucarioticos |
| EP1034262B1 (en) | 1997-11-18 | 2005-08-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
| US6453242B1 (en) | 1999-01-12 | 2002-09-17 | Sangamo Biosciences, Inc. | Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites |
| US6794136B1 (en) | 2000-11-20 | 2004-09-21 | Sangamo Biosciences, Inc. | Iterative optimization in the design of binding proteins |
| JP4808880B2 (ja) | 1999-07-23 | 2011-11-02 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | バクテリオファージphic31組み換え系による真核細胞におけるdna組み換え |
| WO2001011058A1 (en) | 1999-08-09 | 2001-02-15 | Monsanto Technology Llc | Novel cloning methods and vectors |
| US7060499B1 (en) | 1999-09-30 | 2006-06-13 | Izumu Saito | DNA containing variant FRT sequences |
| US6511808B2 (en) | 2000-04-28 | 2003-01-28 | Sangamo Biosciences, Inc. | Methods for designing exogenous regulatory molecules |
| US20030082561A1 (en) | 2000-07-21 | 2003-05-01 | Takashi Sera | Zinc finger domain recognition code and uses thereof |
| WO2002057293A2 (en) | 2001-01-22 | 2002-07-25 | Sangamo Biosciences, Inc. | Modified zinc finger binding proteins |
| GB0108491D0 (en) | 2001-04-04 | 2001-05-23 | Gendaq Ltd | Engineering zinc fingers |
| AT5356U1 (de) | 2001-07-16 | 2002-06-25 | Uv & P Umweltman Verfahrenstec | Verfahren zur immobilisierung von verbrennungsrückständen |
| EP1421187B1 (en) * | 2001-07-27 | 2007-10-10 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| WO2003078619A1 (en) | 2002-03-15 | 2003-09-25 | Cellectis | Hybrid and single chain meganucleases and use thereof |
| WO2009095742A1 (en) | 2008-01-31 | 2009-08-06 | Cellectis | New i-crei derived single-chain meganuclease and uses thereof |
| AU2003218382B2 (en) | 2002-03-21 | 2007-12-13 | Sangamo Therapeutics, Inc. | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
| AU2003290518A1 (en) | 2002-09-06 | 2004-04-23 | Fred Hutchinson Cancer Research Center | Methods and compositions concerning designed highly-specific nucleic acid binding proteins |
| JP2006518372A (ja) | 2003-01-28 | 2006-08-10 | セレクティス | 脊椎動物の体組織においてエクスビボかつイントトで相同的組換えを誘発するためのメガヌクレアーゼの使用およびその応用 |
| EP2927318B1 (en) | 2003-08-08 | 2020-05-20 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
| US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
| EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
| DK1863909T3 (da) | 2005-03-15 | 2010-08-30 | Cellectis | I-CreI-meganukleasevarianter med modificeret specificitet, fremgangsmåde til fremstilling og anvendelser deraf |
| WO2006097784A1 (en) | 2005-03-15 | 2006-09-21 | Cellectis | I-crei meganuclease variants with modified specificity, method of preparation and uses thereof |
| WO2007034262A1 (en) | 2005-09-19 | 2007-03-29 | Cellectis | Heterodimeric meganucleases and use thereof |
| WO2007060495A1 (en) | 2005-10-25 | 2007-05-31 | Cellectis | I-crei homing endonuclease variants having novel cleavage specificity and use thereof |
| WO2007049095A1 (en) | 2005-10-25 | 2007-05-03 | Cellectis | Laglidadg homing endonuclease variants having mutations in two functional subdomains and use thereof |
| KR101439568B1 (ko) | 2006-08-11 | 2014-09-12 | 다우 아그로사이언시즈 엘엘씨 | 아연 손가락 뉴클레아제-매개 상동 재조합 |
| WO2009001159A1 (en) | 2007-06-25 | 2008-12-31 | Cellectis | Method for enhancing the cleavage activity of i-crei derived meganucleases |
| WO2008152524A2 (en) | 2007-06-06 | 2008-12-18 | Cellectis | Method for enhancing the cleavage activity of i-crei derived meganucleases |
| CA2700170A1 (en) | 2007-09-27 | 2009-04-02 | Sangamo Biosciences, Inc. | Genomic editing in zebrafish using zinc finger nucleases |
| CA2703045C (en) | 2007-10-25 | 2017-02-14 | Sangamo Biosciences, Inc. | Methods and compositions for targeted integration |
| WO2010001189A1 (en) | 2008-07-03 | 2010-01-07 | Cellectis | The crystal structure of i-dmoi in complex with its dna target, improved chimeric meganucleases and uses thereof |
| EP2329017A2 (en) | 2008-08-04 | 2011-06-08 | Cellectis | Novel method to generate meganucleases with altered characteristics |
| EP2180058A1 (en) | 2008-10-23 | 2010-04-28 | Cellectis | Meganuclease recombination system |
| WO2010059763A1 (en) | 2008-11-19 | 2010-05-27 | Amyris Biotechnologies, Inc. | Compositions and methods for the assembly of polynucleotides |
| AU2009322964B2 (en) | 2008-12-04 | 2014-10-09 | Sangamo Therapeutics, Inc. | Genome editing in rats using zinc-finger nucleases |
| EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
| US8586363B2 (en) | 2009-12-10 | 2013-11-19 | Regents Of The University Of Minnesota | TAL effector-mediated DNA modification |
| CA2787594C (en) * | 2010-01-22 | 2019-02-26 | Dow Agrosciences Llc | Engineered landing pads for gene targeting in plants |
| US7919605B1 (en) | 2010-08-30 | 2011-04-05 | Amyris, Inc. | Nucleic acids, compositions and methods for the excision of target nucleic acids |
| WO2012138927A2 (en) | 2011-04-05 | 2012-10-11 | Philippe Duchateau | Method for the generation of compact tale-nucleases and uses thereof |
| PT2702160T (pt) | 2011-04-27 | 2020-07-30 | Amyris Inc | Métodos para modificação genómica |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| US9315806B2 (en) * | 2012-06-29 | 2016-04-19 | Massachusetts Institute Of Technology | Massively parallel combinatorial genetics |
| CA2915467A1 (en) | 2013-06-19 | 2014-12-24 | Sigma Aldrich Co. Llc | Targeted integration |
| US9476065B2 (en) * | 2013-12-19 | 2016-10-25 | Amyris, Inc. | Methods for genomic integration |
| WO2017075529A1 (en) * | 2015-10-29 | 2017-05-04 | The Research Foundation For The State University Of New York | Genomic combinatorial screening platform |
-
2017
- 2017-05-18 EP EP23150787.2A patent/EP4219731A3/en active Pending
- 2017-05-18 JP JP2018560571A patent/JP7093310B2/ja active Active
- 2017-05-18 US US16/302,079 patent/US11293033B2/en active Active
- 2017-05-18 EP EP17728315.7A patent/EP3458595A2/en not_active Withdrawn
- 2017-05-18 WO PCT/US2017/033369 patent/WO2017201311A2/en not_active Ceased
-
2022
- 2022-03-09 US US17/690,429 patent/US12110498B2/en active Active
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