JP2020517299A5 - - Google Patents
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- Publication number
- JP2020517299A5 JP2020517299A5 JP2020507491A JP2020507491A JP2020517299A5 JP 2020517299 A5 JP2020517299 A5 JP 2020517299A5 JP 2020507491 A JP2020507491 A JP 2020507491A JP 2020507491 A JP2020507491 A JP 2020507491A JP 2020517299 A5 JP2020517299 A5 JP 2020517299A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- donor
- sequence
- repair template
- double
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108020004414 DNA Proteins 0.000 claims 20
- 238000000034 method Methods 0.000 claims 17
- 230000033616 DNA repair Effects 0.000 claims 9
- 210000003527 eukaryotic cell Anatomy 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 7
- 238000010362 genome editing Methods 0.000 claims 6
- 230000004048 modification Effects 0.000 claims 6
- 238000012986 modification Methods 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 238000010356 CRISPR-Cas9 genome editing Methods 0.000 claims 3
- 230000005782 double-strand break Effects 0.000 claims 3
- 108091033409 CRISPR Proteins 0.000 claims 2
- 108020005004 Guide RNA Proteins 0.000 claims 2
- 108091028113 Trans-activating crRNA Proteins 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 108020005065 3' Flanking Region Proteins 0.000 claims 1
- 108020005029 5' Flanking Region Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 210000004602 germ cell Anatomy 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 230000002103 transcriptional effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/491,125 | 2017-04-19 | ||
| US15/491,125 US11834670B2 (en) | 2017-04-19 | 2017-04-19 | Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences |
| PCT/US2018/028366 WO2018195313A1 (en) | 2017-04-19 | 2018-04-19 | Site-specific dna modification using a donor dna repair template having tandem repeat sequences |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020517299A JP2020517299A (ja) | 2020-06-18 |
| JP2020517299A5 true JP2020517299A5 (enExample) | 2021-05-27 |
| JP7292258B2 JP7292258B2 (ja) | 2023-06-16 |
Family
ID=62196680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020507491A Active JP7292258B2 (ja) | 2017-04-19 | 2018-04-19 | 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11834670B2 (enExample) |
| EP (1) | EP3612630B1 (enExample) |
| JP (1) | JP7292258B2 (enExample) |
| CN (1) | CN110709514B (enExample) |
| CA (1) | CA3062154A1 (enExample) |
| WO (1) | WO2018195313A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| CA2999500A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| EP3433363A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| EP4047092B1 (en) | 2016-04-13 | 2025-07-30 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| EP3652312A1 (en) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| US20200370070A1 (en) * | 2017-11-17 | 2020-11-26 | The Johns Hopkins University | Compositions and methods for efficient genome editing |
| KR20210045360A (ko) | 2018-05-16 | 2021-04-26 | 신테고 코포레이션 | 가이드 rna 설계 및 사용을 위한 방법 및 시스템 |
| BR112022001230A2 (pt) * | 2019-07-23 | 2022-03-15 | Pioneer Hi Bred Int | Estratégia de design de doador para edição de genoma crispr-cas9 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648245A (en) * | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| WO2009022656A1 (ja) * | 2007-08-10 | 2009-02-19 | Toto Ltd. | 組換え哺乳動物細胞、組換え哺乳動物細胞の製造方法、および目的タンパク質の生産方法 |
| US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
| JP2013505013A (ja) * | 2009-09-18 | 2013-02-14 | セレクシス エス．エー． | 強化導入遺伝子発現およびプロセッシングの産物および方法 |
| US9273324B2 (en) * | 2010-12-05 | 2016-03-01 | Andrew S. Belmont | Recombinant gene expression |
| EP2718446A2 (en) | 2011-06-07 | 2014-04-16 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Improved recombination efficiency by inhibition of nhej dna repair |
| FI3597749T3 (fi) * | 2012-05-25 | 2023-10-09 | Univ California | Menetelmiä ja koostumuksia rna-ohjattua kohde-dna-modifikaatiota varten ja rna-ohjattua transkription modulaatiota varten |
| PT3363902T (pt) | 2012-12-06 | 2019-12-19 | Sigma Aldrich Co Llc | Modificação e regulação de genoma baseadas em crispr |
| EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| US9328382B2 (en) * | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| US20150067922A1 (en) | 2013-05-30 | 2015-03-05 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| WO2016021973A1 (ko) | 2014-08-06 | 2016-02-11 | 주식회사 툴젠 | 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정 |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| EP4335918A3 (en) * | 2015-04-03 | 2024-04-17 | Dana-Farber Cancer Institute, Inc. | Composition and methods of genome editing of b-cells |
-
2017
- 2017-04-19 US US15/491,125 patent/US11834670B2/en active Active
-
2018
- 2018-04-19 CA CA3062154A patent/CA3062154A1/en active Pending
- 2018-04-19 CN CN201880025960.XA patent/CN110709514B/zh active Active
- 2018-04-19 EP EP18725682.1A patent/EP3612630B1/en active Active
- 2018-04-19 JP JP2020507491A patent/JP7292258B2/ja active Active
- 2018-04-19 WO PCT/US2018/028366 patent/WO2018195313A1/en not_active Ceased
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