JP2020517299A5 - - Google Patents
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- Publication number
- JP2020517299A5 JP2020517299A5 JP2020507491A JP2020507491A JP2020517299A5 JP 2020517299 A5 JP2020517299 A5 JP 2020517299A5 JP 2020507491 A JP2020507491 A JP 2020507491A JP 2020507491 A JP2020507491 A JP 2020507491A JP 2020517299 A5 JP2020517299 A5 JP 2020517299A5
- Authority
- JP
- Japan
- Prior art keywords
- dna
- donor
- sequence
- repair template
- double
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 108020004414 DNA Proteins 0.000 claims 20
- 238000000034 method Methods 0.000 claims 17
- 230000033616 DNA repair Effects 0.000 claims 9
- 210000003527 eukaryotic cell Anatomy 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 7
- 238000010362 genome editing Methods 0.000 claims 6
- 230000004048 modification Effects 0.000 claims 6
- 238000012986 modification Methods 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 238000010356 CRISPR-Cas9 genome editing Methods 0.000 claims 3
- 230000005782 double-strand break Effects 0.000 claims 3
- 108091033409 CRISPR Proteins 0.000 claims 2
- 108020005004 Guide RNA Proteins 0.000 claims 2
- 108091028113 Trans-activating crRNA Proteins 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- 230000001105 regulatory effect Effects 0.000 claims 2
- 108020005065 3' Flanking Region Proteins 0.000 claims 1
- 108020005029 5' Flanking Region Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 239000012636 effector Substances 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 210000004602 germ cell Anatomy 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 230000002103 transcriptional effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15/491,125 US11834670B2 (en) | 2017-04-19 | 2017-04-19 | Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences |
| US15/491,125 | 2017-04-19 | ||
| PCT/US2018/028366 WO2018195313A1 (en) | 2017-04-19 | 2018-04-19 | Site-specific dna modification using a donor dna repair template having tandem repeat sequences |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020517299A JP2020517299A (ja) | 2020-06-18 |
| JP2020517299A5 true JP2020517299A5 (enExample) | 2021-05-27 |
| JP7292258B2 JP7292258B2 (ja) | 2023-06-16 |
Family
ID=62196680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020507491A Active JP7292258B2 (ja) | 2017-04-19 | 2018-04-19 | 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11834670B2 (enExample) |
| EP (1) | EP3612630B1 (enExample) |
| JP (1) | JP7292258B2 (enExample) |
| CN (1) | CN110709514B (enExample) |
| CA (1) | CA3062154A1 (enExample) |
| WO (1) | WO2018195313A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| US11680268B2 (en) | 2014-11-07 | 2023-06-20 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| EP3786294A1 (en) | 2015-09-24 | 2021-03-03 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US11597924B2 (en) | 2016-03-25 | 2023-03-07 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| WO2017180694A1 (en) | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Cas9 fusion molecules gene editing systems, and methods of use thereof |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| US10350307B2 (en) * | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| US20200370070A1 (en) * | 2017-11-17 | 2020-11-26 | The Johns Hopkins University | Compositions and methods for efficient genome editing |
| CN112654710A (zh) | 2018-05-16 | 2021-04-13 | 辛瑟高公司 | 用于指导rna设计和使用的方法和系统 |
| BR112022001230A2 (pt) * | 2019-07-23 | 2022-03-15 | Pioneer Hi Bred Int | Estratégia de design de doador para edição de genoma crispr-cas9 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648245A (en) * | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| EP2189529B1 (en) * | 2007-08-10 | 2017-01-11 | Toto Ltd. | Method of producing recombinant mammalian cells and method of producing target protein |
| US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
| EP2478108A2 (en) * | 2009-09-18 | 2012-07-25 | Selexis S.A. | Products and methods for enhanced transgene expression and processing |
| US9273324B2 (en) * | 2010-12-05 | 2016-03-01 | Andrew S. Belmont | Recombinant gene expression |
| EP2718446A2 (en) | 2011-06-07 | 2014-04-16 | Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Improved recombination efficiency by inhibition of nhej dna repair |
| PL4289948T3 (pl) * | 2012-05-25 | 2025-06-02 | The Regents Of The University Of California | Sposoby i kompozycje do modyfikacji kierowanego na rna docelowego dna i do nakierowanej na rna modulacji transkrypcji |
| CN105142669B (zh) | 2012-12-06 | 2018-07-03 | 西格马-奥尔德里奇有限责任公司 | 基于crispr的基因组修饰和调控 |
| CN105658796B (zh) | 2012-12-12 | 2021-10-26 | 布罗德研究所有限公司 | 用于序列操纵的crispr-cas组分系统、方法以及组合物 |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| US9328382B2 (en) * | 2013-03-15 | 2016-05-03 | Complete Genomics, Inc. | Multiple tagging of individual long DNA fragments |
| WO2014194190A1 (en) | 2013-05-30 | 2014-12-04 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| WO2016021973A1 (ko) | 2014-08-06 | 2016-02-11 | 주식회사 툴젠 | 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정 |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| US11680268B2 (en) | 2014-11-07 | 2023-06-20 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
| ES2959608T3 (es) * | 2015-04-03 | 2024-02-27 | Dana Farber Cancer Inst Inc | Composición y métodos de edición del genoma de células B |
-
2017
- 2017-04-19 US US15/491,125 patent/US11834670B2/en active Active
-
2018
- 2018-04-19 CN CN201880025960.XA patent/CN110709514B/zh active Active
- 2018-04-19 WO PCT/US2018/028366 patent/WO2018195313A1/en not_active Ceased
- 2018-04-19 JP JP2020507491A patent/JP7292258B2/ja active Active
- 2018-04-19 EP EP18725682.1A patent/EP3612630B1/en active Active
- 2018-04-19 CA CA3062154A patent/CA3062154A1/en active Pending
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