JP7292258B2 - 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 - Google Patents

縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 Download PDF

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JP7292258B2
JP7292258B2 JP2020507491A JP2020507491A JP7292258B2 JP 7292258 B2 JP7292258 B2 JP 7292258B2 JP 2020507491 A JP2020507491 A JP 2020507491A JP 2020507491 A JP2020507491 A JP 2020507491A JP 7292258 B2 JP7292258 B2 JP 7292258B2
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dna
donor
repair template
sequence
rca
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JP2020517299A (ja
JP2020517299A5 (enExample
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ジョン・リチャード・ネルソン
ロバート・スコット・ダシー
パトリック・マッコイ・スプーナー
ジョン・アンソニー・シール
リサ・アン・ロウリー
アンジャ・ジョシファ・スミス
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Global Life Sciences Solutions USA LLC
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Amersham Biosciences Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020507491A 2017-04-19 2018-04-19 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変 Active JP7292258B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/491,125 2017-04-19
US15/491,125 US11834670B2 (en) 2017-04-19 2017-04-19 Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences
PCT/US2018/028366 WO2018195313A1 (en) 2017-04-19 2018-04-19 Site-specific dna modification using a donor dna repair template having tandem repeat sequences

Publications (3)

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JP2020517299A JP2020517299A (ja) 2020-06-18
JP2020517299A5 JP2020517299A5 (enExample) 2021-05-27
JP7292258B2 true JP7292258B2 (ja) 2023-06-16

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JP2020507491A Active JP7292258B2 (ja) 2017-04-19 2018-04-19 縦列反復配列を有するドナーｄｎａ修復鋳型を使用する部位特異的なｄｎａ改変

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US (1) US11834670B2 (enExample)
EP (1) EP3612630B1 (enExample)
JP (1) JP7292258B2 (enExample)
CN (1) CN110709514B (enExample)
CA (1) CA3062154A1 (enExample)
WO (1) WO2018195313A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
US20200370070A1 (en) * 2017-11-17 2020-11-26 The Johns Hopkins University Compositions and methods for efficient genome editing
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
BR112022001230A2 (pt) * 2019-07-23 2022-03-15 Pioneer Hi Bred Int Estratégia de design de doador para edição de genoma crispr-cas9

Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2016500262A (ja) 2012-12-12 2016-01-12 ザ・ブロード・インスティテュート・インコーポレイテッ 遺伝子産物の発現を変更するためのＣＲＩＳＰＲ−Ｃａｓ系および方法
US20160046985A1 (en) 2013-03-15 2016-02-18 Complete Genomics, Inc. Multiple tagging of long dna fragments

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US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
EP3176263A1 (en) * 2007-08-10 2017-06-07 Toto Ltd. Method of producing recombinant mammalian cells
US8921072B2 (en) 2008-09-02 2014-12-30 General Electric Compnay Methods to generate DNA mini-circles
CN102575264A (zh) * 2009-09-18 2012-07-11 瑟莱克斯公司 增加的转基因表达和加工的产品和方法
US9273324B2 (en) * 2010-12-05 2016-03-01 Andrew S. Belmont Recombinant gene expression
WO2012168307A2 (en) 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
ES2728782T3 (es) * 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
DK3363902T3 (da) 2012-12-06 2020-01-02 Sigma Aldrich Co Llc Crispr-baseret genommodifikation og -regulering
KR102955556B1 (ko) 2012-12-12 2026-04-22 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물
US20150067922A1 (en) 2013-05-30 2015-03-05 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
WO2016021973A1 (ko) 2014-08-06 2016-02-11 주식회사 툴젠 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2981077A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b-cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016500262A (ja) 2012-12-12 2016-01-12 ザ・ブロード・インスティテュート・インコーポレイテッ 遺伝子産物の発現を変更するためのＣＲＩＳＰＲ−Ｃａｓ系および方法
US20160046985A1 (en) 2013-03-15 2016-02-18 Complete Genomics, Inc. Multiple tagging of long dna fragments

Non-Patent Citations (2)

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Title
Cell Reports，2016年，Vol.14，p.2263-2272
Nucleosides, Nucleotides, and Nucleic Acids，2008年，Vol.27，p.224-243

Also Published As

Publication number Publication date
JP2020517299A (ja) 2020-06-18
CN110709514A (zh) 2020-01-17
CA3062154A1 (en) 2018-10-25
US20180305718A1 (en) 2018-10-25
EP3612630B1 (en) 2023-08-30
US11834670B2 (en) 2023-12-05
CN110709514B (zh) 2024-03-08
EP3612630A1 (en) 2020-02-26
WO2018195313A1 (en) 2018-10-25

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