CN110709514B - 使用具有串联重复序列的供体dna修复模板的位点特异性dna修饰 - Google Patents

使用具有串联重复序列的供体dna修复模板的位点特异性dna修饰 Download PDF

Info

Publication number
CN110709514B
CN110709514B CN201880025960.XA CN201880025960A CN110709514B CN 110709514 B CN110709514 B CN 110709514B CN 201880025960 A CN201880025960 A CN 201880025960A CN 110709514 B CN110709514 B CN 110709514B
Authority
CN
China
Prior art keywords
dna
donor
sequence
endogenous target
donor dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201880025960.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN110709514A (zh
Inventor
J.R.纳尔逊
R.S.达西
P.M.思普奈尔
J.A.席尔
L.A.罗威利
A.J.史密斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Globegroup Life Technology Consulting America Co ltd
Original Assignee
Globegroup Life Technology Consulting America Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Globegroup Life Technology Consulting America Co ltd filed Critical Globegroup Life Technology Consulting America Co ltd
Publication of CN110709514A publication Critical patent/CN110709514A/zh
Application granted granted Critical
Publication of CN110709514B publication Critical patent/CN110709514B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880025960.XA 2017-04-19 2018-04-19 使用具有串联重复序列的供体dna修复模板的位点特异性dna修饰 Active CN110709514B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15/491125 2017-04-19
US15/491,125 US11834670B2 (en) 2017-04-19 2017-04-19 Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences
PCT/US2018/028366 WO2018195313A1 (en) 2017-04-19 2018-04-19 Site-specific dna modification using a donor dna repair template having tandem repeat sequences

Publications (2)

Publication Number Publication Date
CN110709514A CN110709514A (zh) 2020-01-17
CN110709514B true CN110709514B (zh) 2024-03-08

Family

ID=62196680

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880025960.XA Active CN110709514B (zh) 2017-04-19 2018-04-19 使用具有串联重复序列的供体dna修复模板的位点特异性dna修饰

Country Status (6)

Country Link
US (1) US11834670B2 (enExample)
EP (1) EP3612630B1 (enExample)
JP (1) JP7292258B2 (enExample)
CN (1) CN110709514B (enExample)
CA (1) CA3062154A1 (enExample)
WO (1) WO2018195313A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
CA2999500A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
US20200370070A1 (en) * 2017-11-17 2020-11-26 The Johns Hopkins University Compositions and methods for efficient genome editing
CN112654710A (zh) 2018-05-16 2021-04-13 辛瑟高公司 用于指导rna设计和使用的方法和系统
BR112022001230A2 (pt) * 2019-07-23 2022-03-15 Pioneer Hi Bred Int Estratégia de design de doador para edição de genoma crispr-cas9

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012168307A2 (en) * 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
WO2016057961A1 (en) * 2014-10-10 2016-04-14 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) * 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
EP3176263A1 (en) * 2007-08-10 2017-06-07 Toto Ltd. Method of producing recombinant mammalian cells
US8921072B2 (en) 2008-09-02 2014-12-30 General Electric Compnay Methods to generate DNA mini-circles
CN102575264A (zh) * 2009-09-18 2012-07-11 瑟莱克斯公司 增加的转基因表达和加工的产品和方法
US9273324B2 (en) * 2010-12-05 2016-03-01 Andrew S. Belmont Recombinant gene expression
ES2728782T3 (es) * 2012-05-25 2019-10-28 Univ California Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN
DK3363902T3 (da) 2012-12-06 2020-01-02 Sigma Aldrich Co Llc Crispr-baseret genommodifikation og -regulering
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
KR102955556B1 (ko) 2012-12-12 2026-04-22 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물
US9328382B2 (en) * 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
US20150067922A1 (en) 2013-05-30 2015-03-05 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
WO2016021973A1 (ko) 2014-08-06 2016-02-11 주식회사 툴젠 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정
CA2981077A1 (en) * 2015-04-03 2016-10-06 Dana-Farber Cancer Institute, Inc. Composition and methods of genome editing of b-cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012168307A2 (en) * 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
WO2016057961A1 (en) * 2014-10-10 2016-04-14 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2016073990A2 (en) * 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Development and Applications of CRISPR-Cas9 for Genome Engineering;Patrick D. Hsu;《Cell》;第1262-1278页 *
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila;Gratz, Scott J.;《Genetics》;第961-971页 *
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells;Van Trung;《Nature biotechnology》;第543-548页 *
Self-Assembled DNA Nanoclews for the Efficient Delivery of CRISPR–Cas9 for Genome Editing;Wujin Sun;《Angew.Chem.》;第12197–12201页 *
Stimulation of Homology-Directed Repair at ISceI- Induced DNA Breaks during the Permissive Life Cycle of Human Cytomegalovirus;Kulkarni, A. S.;《Journal of Virology》;第1-2页 *

Also Published As

Publication number Publication date
JP7292258B2 (ja) 2023-06-16
JP2020517299A (ja) 2020-06-18
CN110709514A (zh) 2020-01-17
CA3062154A1 (en) 2018-10-25
US20180305718A1 (en) 2018-10-25
EP3612630B1 (en) 2023-08-30
US11834670B2 (en) 2023-12-05
EP3612630A1 (en) 2020-02-26
WO2018195313A1 (en) 2018-10-25

Similar Documents

Publication Publication Date Title
US11834670B2 (en) Site-specific DNA modification using a donor DNA repair template having tandem repeat sequences
JP7605852B2 (ja) クラスｉｉのｖ型ｃｒｉｓｐｒ系
JP2026065115A (ja) Ｓ．ピオゲネスｃａｓ９変異遺伝子及びこれによってコードされるポリペプチド
CN102317475B (zh) 单链dna的不依赖模板的连接
ES2550237T3 (es) Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
CN110382710B (zh) 构建核酸分子拷贝的方法
KR20220159498A (ko) Crispr 하이브리드 dna/rna 폴리뉴클레오티드 및 사용 방법
US20220333129A1 (en) A nucleic acid delivery vector comprising a circular single stranded polynucleotide
CN111094574A (zh) 使用包括硫代磷酸酯化的核苷酸的双链多联体dna的体内rna或蛋白质表达
US20230122979A1 (en) Methods of sample normalization
WO2023052774A1 (en) Methods for gene editing
US20220372522A1 (en) Compositions and methods for homology-directed recombination
Balke et al. In vitro repair of a defective EGFP transcript and translation into a functional protein
JP5129498B2 (ja) 核酸クローニング法
US20250084484A1 (en) Methods and compositions for transcriptome analysis
US20260085308A1 (en) Methods for generating cdna library from rna
US20250002906A1 (en) ENGINEERED sgRNA DESIGN AND PREPARATION METHOD
WO2026099450A1 (en) Protected dna and methods for the production thereof
CN120173913A (zh) 新型Cas12a核酸酶BEST3及其应用
WO2024138131A1 (en) Expanding applications of zgtc alphabet in protein expression and gene editing
CN118355129A (zh) 捕获crispr核酸内切酶切割产物的方法
CN121950793A (zh) 一种工程化改造的IscB-ωRNA系统及用途

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant