JP2022046466A5 - - Google Patents
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- JP2022046466A5 JP2022046466A5 JP2021193224A JP2021193224A JP2022046466A5 JP 2022046466 A5 JP2022046466 A5 JP 2022046466A5 JP 2021193224 A JP2021193224 A JP 2021193224A JP 2021193224 A JP2021193224 A JP 2021193224A JP 2022046466 A5 JP2022046466 A5 JP 2022046466A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- adapter
- sample
- ligated
- guided nuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 17
- 150000007523 nucleic acids Chemical group 0.000 claims 14
- 108020004707 nucleic acids Proteins 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 10
- 239000000523 sample Substances 0.000 claims 7
- 101710163270 Nuclease Proteins 0.000 claims 3
- 230000008685 targeting Effects 0.000 claims 3
- 108091033409 CRISPR Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 108091092878 Microsatellite Proteins 0.000 claims 2
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 101150069031 CSN2 gene Proteins 0.000 claims 1
- 101150074775 Csf1 gene Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 206010064571 Gene mutation Diseases 0.000 claims 1
- 108020005004 Guide RNA Proteins 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108700020796 Oncogene Proteins 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 101150055601 cops2 gene Proteins 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000012217 deletion Methods 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 238000003205 genotyping method Methods 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562207359P | 2015-08-19 | 2015-08-19 | |
| US62/207,359 | 2015-08-19 | ||
| JP2018508166A JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| PCT/US2016/047631 WO2017031360A1 (en) | 2015-08-19 | 2016-08-18 | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Division JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2022046466A JP2022046466A (ja) | 2022-03-23 |
| JP2022046466A5 true JP2022046466A5 (enExample) | 2022-06-09 |
Family
ID=58050956
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| JP2021193224A Pending JP2022046466A (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10538758B2 (enExample) |
| EP (2) | EP3985111A1 (enExample) |
| JP (2) | JP6986509B2 (enExample) |
| CN (1) | CN108138176B (enExample) |
| AU (1) | AU2016308283B2 (enExample) |
| CA (1) | CA2995983A1 (enExample) |
| DK (1) | DK3337898T3 (enExample) |
| WO (1) | WO2017031360A1 (enExample) |
Families Citing this family (59)
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| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| WO2015122967A1 (en) | 2014-02-13 | 2015-08-20 | Clontech Laboratories, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CA2971444A1 (en) * | 2014-12-20 | 2016-06-23 | Arc Bio, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
| CA2995983A1 (en) | 2015-08-19 | 2017-02-23 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| EP3386550B1 (en) | 2015-12-07 | 2021-01-20 | Arc Bio, LLC | Methods for the making and using of guide nucleic acids |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
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| EP3885448B1 (en) * | 2016-09-02 | 2023-03-22 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
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| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
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| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| US20200190508A1 (en) * | 2017-06-07 | 2020-06-18 | Arc Bio, Llc | Creation and use of guide nucleic acids |
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| WO2018231952A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Plasma/serum target enrichment |
| US10081829B1 (en) * | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| CA3069938A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Isolation of target nucleic acids |
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| US10947599B2 (en) | 2017-06-13 | 2021-03-16 | Genetics Research, Llc | Tumor mutation burden |
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| KR102634727B1 (ko) * | 2017-06-14 | 2024-02-07 | 위스콘신 얼럼나이 리서어치 화운데이션 | 변형된 가이드 rna, crispr-리보뉴클레오프로테인 복합체 및 사용 방법 |
| CN107190008A (zh) * | 2017-07-19 | 2017-09-22 | 苏州吉赛基因测序科技有限公司 | 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| KR20200074104A (ko) * | 2017-10-04 | 2020-06-24 | 더 브로드 인스티튜트, 인코퍼레이티드 | 표적화된 핵산 편집을 위한 시스템, 방법, 및 조성물 |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2019237032A1 (en) | 2018-06-07 | 2019-12-12 | Arc Bio, Llc | Compositions and methods for making guide nucleic acids |
| CN109182454A (zh) * | 2018-08-22 | 2019-01-11 | 杭州恺思医疗器械有限公司 | 一种捕获基因组特定dna片段的方法 |
| CN109837273B (zh) * | 2018-09-17 | 2021-06-29 | 东南大学 | 一种crispr辅助dna靶向富集方法及其应用 |
| AU2019356016A1 (en) | 2018-10-04 | 2021-05-13 | Arc Bio, Llc | Normalization controls for managing low sample inputs in next generation sequencing |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN110205318A (zh) * | 2019-05-15 | 2019-09-06 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas去除宿主基因组DNA的宏基因组提取方法 |
| GB201913997D0 (en) * | 2019-09-27 | 2019-11-13 | Oxford Nanopore Tech Ltd | Method |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021141970A1 (en) * | 2020-01-06 | 2021-07-15 | Pairwise Plants Services, Inc. | Recruitment of dna polymerase for templated editing |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| WO2021252867A2 (en) * | 2020-06-12 | 2021-12-16 | Qiagen Sciences, Llc | Methods of enriching for target nucelic acid molecules and uses thereof |
| CN112159822A (zh) * | 2020-09-30 | 2021-01-01 | 扬州大学 | 一种PS转座酶与CRISPR/dCpf1融合蛋白表达载体及其介导的定点整合方法 |
| WO2022076313A1 (en) * | 2020-10-06 | 2022-04-14 | Genetics Research, Llc | Nucleic acid enrichment method |
| CN115927539A (zh) * | 2022-09-14 | 2023-04-07 | 首都体育学院 | 靶标核酸富集方法及其试剂盒和应用 |
| WO2025132779A2 (en) * | 2023-12-22 | 2025-06-26 | F. Hoffmann-La Roche Ag | Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion |
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| US177444A (en) * | 1876-05-16 | Improvement in transplanters | ||
| US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
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| EP1606417A2 (en) * | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna |
| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| US20090318305A1 (en) | 2008-06-18 | 2009-12-24 | Xi Erick Lin | Methods for selectively capturing and amplifying exons or targeted genomic regions from biological samples |
| WO2012051327A2 (en) | 2010-10-12 | 2012-04-19 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements |
| CA2821299C (en) * | 2010-11-05 | 2019-02-12 | Frank J. Steemers | Linking sequence reads using paired code tags |
| US9249460B2 (en) | 2011-09-09 | 2016-02-02 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
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| WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| FI3597749T3 (fi) | 2012-05-25 | 2023-10-09 | Univ California | Menetelmiä ja koostumuksia rna-ohjattua kohde-dna-modifikaatiota varten ja rna-ohjattua transkription modulaatiota varten |
| WO2013191775A2 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US10847248B2 (en) | 2012-08-10 | 2020-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Techniques for determining haplotype by population genotype and sequence data |
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| WO2014189957A2 (en) | 2013-05-23 | 2014-11-27 | The Board Of Trustees Of The Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| US9873907B2 (en) * | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
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| CN108778343A (zh) | 2015-10-16 | 2018-11-09 | 天普大学-联邦高等教育系统 | 利用cpf1进行rna向导的基因编辑的方法和组合物 |
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| AU2017312953A1 (en) | 2016-08-16 | 2019-01-24 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (flash) |
| US20180051320A1 (en) | 2016-08-22 | 2018-02-22 | The Regents Of The University Of California | Depletion of abundant sequences by hybridization (dash) |
| CN109923217B (zh) | 2016-10-13 | 2023-06-16 | 生物梅里埃公司 | 宏基因组样品中病原体的鉴定和抗生素表征 |
-
2016
- 2016-08-18 CA CA2995983A patent/CA2995983A1/en active Pending
- 2016-08-18 WO PCT/US2016/047631 patent/WO2017031360A1/en not_active Ceased
- 2016-08-18 JP JP2018508166A patent/JP6986509B2/ja active Active
- 2016-08-18 US US15/520,052 patent/US10538758B2/en active Active
- 2016-08-18 EP EP21188035.6A patent/EP3985111A1/en active Pending
- 2016-08-18 DK DK16837863.6T patent/DK3337898T3/da active
- 2016-08-18 CN CN201680057464.3A patent/CN108138176B/zh active Active
- 2016-08-18 AU AU2016308283A patent/AU2016308283B2/en active Active
- 2016-08-18 EP EP16837863.6A patent/EP3337898B1/en active Active
-
2019
- 2019-12-02 US US16/700,493 patent/US20200087652A1/en not_active Abandoned
-
2021
- 2021-11-29 JP JP2021193224A patent/JP2022046466A/ja active Pending
-
2023
- 2023-02-01 US US18/163,196 patent/US20240132872A1/en active Pending
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