AU2016308283B2 - Capture of nucleic acids using a nucleic acid-guided nuclease-based system - Google Patents

Capture of nucleic acids using a nucleic acid-guided nuclease-based system Download PDF

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AU2016308283B2
AU2016308283B2 AU2016308283A AU2016308283A AU2016308283B2 AU 2016308283 B2 AU2016308283 B2 AU 2016308283B2 AU 2016308283 A AU2016308283 A AU 2016308283A AU 2016308283 A AU2016308283 A AU 2016308283A AU 2016308283 B2 AU2016308283 B2 AU 2016308283B2
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nucleic acid
nickase
dna
interest
sample
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AU2016308283A1 (en
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Meredith L. CARPENTER
Stephane B. Gourguechon
Eric HARNESS
David SINCLAIA
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ARC Bio LLC
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ARC Bio LLC
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

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  • Crystallography & Structural Chemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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AU2016308283A 2015-08-19 2016-08-18 Capture of nucleic acids using a nucleic acid-guided nuclease-based system Active AU2016308283B2 (en)

Applications Claiming Priority (3)

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US201562207359P 2015-08-19 2015-08-19
US62/207,359 2015-08-19
PCT/US2016/047631 WO2017031360A1 (en) 2015-08-19 2016-08-18 Capture of nucleic acids using a nucleic acid-guided nuclease-based system

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AU2016308283A1 AU2016308283A1 (en) 2018-03-15
AU2016308283B2 true AU2016308283B2 (en) 2022-04-21

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EP (2) EP3985111A1 (enExample)
JP (2) JP6986509B2 (enExample)
CN (1) CN108138176B (enExample)
AU (1) AU2016308283B2 (enExample)
CA (1) CA2995983A1 (enExample)
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