JP6986509B2 - 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 - Google Patents

核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 Download PDF

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JP6986509B2
JP6986509B2 JP2018508166A JP2018508166A JP6986509B2 JP 6986509 B2 JP6986509 B2 JP 6986509B2 JP 2018508166 A JP2018508166 A JP 2018508166A JP 2018508166 A JP2018508166 A JP 2018508166A JP 6986509 B2 JP6986509 B2 JP 6986509B2
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JP2018529326A (ja
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ステファン ビー． グーギュッチョン，
エリック ハーネス，
デイビッド シンクレア，
メレディス エル． カーペンター，
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アーク バイオ， エルエルシー
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Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
US10150985B2 (en) 2014-02-13 2018-12-11 Takara Bio Usa, Inc. Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3957745A1 (en) * 2014-12-20 2022-02-23 Arc Bio, LLC Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
US10538758B2 (en) 2015-08-19 2020-01-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
WO2017100343A1 (en) 2015-12-07 2017-06-15 Arc Bio, Llc Methods and compositions for the making and using of guide nucleic acids
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3885448B1 (en) * 2016-09-02 2023-03-22 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
US20200190508A1 (en) * 2017-06-07 2020-06-18 Arc Bio, Llc Creation and use of guide nucleic acids
US10947599B2 (en) 2017-06-13 2021-03-16 Genetics Research, Llc Tumor mutation burden
US10527608B2 (en) 2017-06-13 2020-01-07 Genetics Research, Llc Methods for rare event detection
US20180355437A1 (en) * 2017-06-13 2018-12-13 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Plasma/serum target enrichment
CA3069938A1 (en) * 2017-06-13 2018-12-20 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Isolation of target nucleic acids
US20180355417A1 (en) * 2017-06-13 2018-12-13 Genetics Research, Llc, D/B/A Zs Genetics, Inc. Rare nucleic acid detection
EP3638809A4 (en) * 2017-06-13 2021-03-10 Genetics Research, LLC, D/B/A ZS Genetics, Inc. NEGATIVE-POSITIVE ENRICHMENT FOR THE DETECTION OF NUCLEIC ACIDS
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
MX2019015143A (es) * 2017-06-14 2020-07-27 Wisconsin Alumni Res Found Rna guias modificados, complejos crispr-ribonucleoproteina y metodos de uso.
CN107190008A (zh) * 2017-07-19 2017-09-22 苏州吉赛基因测序科技有限公司 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CN111727247A (zh) 2017-10-04 2020-09-29 博德研究所 用于靶向核酸编辑的系统、方法和组合物
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN110157727A (zh) * 2017-12-21 2019-08-23 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
CA3101648A1 (en) 2018-06-07 2019-12-12 Arc Bio, Llc Compositions and methods for making guide nucleic acids
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
CN109182454A (zh) * 2018-08-22 2019-01-11 杭州恺思医疗器械有限公司 一种捕获基因组特定dna片段的方法
CN109837273B (zh) * 2018-09-17 2021-06-29 东南大学 一种crispr辅助dna靶向富集方法及其应用
IL281982B2 (en) 2018-10-04 2025-12-01 Arc Bio Llc Normalization controls for managing low sample inputs in next generation sequencing
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US20220186290A1 (en) 2019-04-09 2022-06-16 Arc Bio, Llc Compositions and methods for nucleotide modification-based depletion
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110205318A (zh) * 2019-05-15 2019-09-06 杭州杰毅生物技术有限公司 基于CRISPR-Cas去除宿主基因组DNA的宏基因组提取方法
GB201913997D0 (en) * 2019-09-27 2019-11-13 Oxford Nanopore Tech Ltd Method
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
AU2021206639A1 (en) * 2020-01-06 2022-07-14 Pairwise Plants Services, Inc. Recruitment of DNA polymerase for templated editing
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
WO2021252867A2 (en) * 2020-06-12 2021-12-16 Qiagen Sciences, Llc Methods of enriching for target nucelic acid molecules and uses thereof
CN112159822A (zh) * 2020-09-30 2021-01-01 扬州大学 一种PS转座酶与CRISPR/dCpf1融合蛋白表达载体及其介导的定点整合方法
US12522865B2 (en) * 2020-10-06 2026-01-13 Harbinger Health, Inc. Nucleic acid enrichment method
CN115927539A (zh) * 2022-09-14 2023-04-07 首都体育学院 靶标核酸富集方法及其试剂盒和应用
WO2025132779A2 (en) * 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US177444A (en) * 1876-05-16 Improvement in transplanters
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
US7166432B2 (en) 1999-03-12 2007-01-23 Integragen Compositions and methods for genetic analysis
EP1606417A2 (en) * 2003-03-07 2005-12-21 Rubicon Genomics Inc. In vitro dna immortalization and whole genome amplification using libraries generated from randomly fragmented dna
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US20090318305A1 (en) 2008-06-18 2009-12-24 Xi Erick Lin Methods for selectively capturing and amplifying exons or targeted genomic regions from biological samples
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
EP2635679B1 (en) * 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
EP2753715A4 (en) 2011-09-09 2015-05-20 Univ Leland Stanford Junior METHOD FOR OBTAINING A SEQUENCE
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US10847248B2 (en) 2012-08-10 2020-11-24 The Board Of Trustees Of The Leland Stanford Junior University Techniques for determining haplotype by population genotype and sequence data
WO2014071070A1 (en) 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
EP2896697B1 (en) * 2012-12-12 2015-09-02 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions for sequence manipulation
WO2014182574A1 (en) 2013-05-04 2014-11-13 The Board Of Trustees Of The Leland Stanford Junior University Enrichment of dna sequencing libraries from samples containing small amounts of target dna
EP4617376A3 (en) 2013-05-23 2025-12-10 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
US11414695B2 (en) * 2013-05-29 2022-08-16 Agilent Technologies, Inc. Nucleic acid enrichment using Cas9
US10421957B2 (en) 2013-07-29 2019-09-24 Agilent Technologies, Inc. DNA assembly using an RNA-programmable nickase
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
CA2929557C (en) 2013-11-07 2023-09-26 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
WO2015075056A1 (en) 2013-11-19 2015-05-28 Thermo Fisher Scientific Baltics Uab Programmable enzymes for isolation of specific dna fragments
US9850525B2 (en) * 2014-01-29 2017-12-26 Agilent Technologies, Inc. CAS9-based isothermal method of detection of specific DNA sequence
EP4467654A3 (en) 2014-02-04 2025-02-19 Jumpcode Genomics, Inc. Genome fractioning
US10150985B2 (en) 2014-02-13 2018-12-11 Takara Bio Usa, Inc. Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same
US9670485B2 (en) 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
WO2015134552A1 (en) 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
US20160053304A1 (en) 2014-07-18 2016-02-25 Whitehead Institute For Biomedical Research Methods Of Depleting Target Sequences Using CRISPR
CN112941065A (zh) 2014-07-21 2021-06-11 亿明达股份有限公司 使用crispr-cas系统的多核苷酸富集
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US20180320226A1 (en) 2014-08-19 2018-11-08 President And Fellows Of Harvard College RNA-Guided Systems For Probing And Mapping Of Nucleic Acids
EP3957745A1 (en) * 2014-12-20 2022-02-23 Arc Bio, LLC Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
CA2986036C (en) 2015-05-18 2022-07-26 Karius, Inc. Compositions and methods for enriching populations of nucleic acids
WO2016196805A1 (en) 2015-06-05 2016-12-08 The Regents Of The University Of California Methods and compositions for generating crispr/cas guide rnas
US10538758B2 (en) 2015-08-19 2020-01-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
US10747899B2 (en) 2015-10-07 2020-08-18 The Board Of Trustees Of The Leland Stanford Junior University Techniques for determining whether an individual is included in ensemble genomic data
CN108778343A (zh) 2015-10-16 2018-11-09 天普大学-联邦高等教育系统 利用cpf1进行rna向导的基因编辑的方法和组合物
EP3433382B1 (en) 2016-03-25 2021-09-01 Karius, Inc. Synthetic nucleic acid spike-ins
US11046995B2 (en) 2016-08-16 2021-06-29 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (FLASH)
US20180051320A1 (en) 2016-08-22 2018-02-22 The Regents Of The University Of California Depletion of abundant sequences by hybridization (dash)
JP7068287B2 (ja) 2016-10-13 2022-05-16 ビオメリュー メタゲノム試料中の病原体の同定と抗生物質の特徴づけ

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